1.Detection of Bartonella henselae DNA by Polymerase Chain Reaction in a Patient with Cat Scratch Disease: A Case Report.
Ju Young CHUNG ; Tae Hee HAN ; Baek Nam KIM ; Young Sam YOO ; Seong Jig LIM
Journal of Korean Medical Science 2005;20(5):888-891
We report a case of cat scratch disease caused by Bartonella henselae in Korea. A 25-yr-old woman developed left cervical lymphadenopathy with history of contact with a dog. The cervical lymphadenopathy persisted for 1 month and resolved gradually and spontaneously. Serologic test was not done during the acute stage of the disease. Immunofluorescent antibody test performed during the convalescent stage was positive for B. henselae. To confirm B. henselae infection, polymerase chain reaction (PCR) analysis using aspirates of cervical lymph node was performed and the presence of B. henselae DNA was demonstrated. This is the first reported case of cat scratch disease in Korea confirmed by PCR for B. henselae DNA.
Adult
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Bartonella henselae/*genetics/*isolation and purification
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Cat-Scratch Disease/*diagnosis/*microbiology
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DNA, Bacterial/*analysis/*genetics
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Female
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Humans
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Polymerase Chain Reaction/*methods
2.A Report of Cat Scratch Disease in Korea Confirmed by PCR Amplification of the 16S-23S rRNA Intergenic Region of Bartonella henselae.
Borum SUH ; Jin Kyoung CHUN ; Dongeun YONG ; Yang Soon LEE ; Seok Hoon JEONG ; Woo Ick YANG ; Dong Soo KIM
The Korean Journal of Laboratory Medicine 2010;30(1):34-37
We report a case of cat scratch disease in an 8-yr-old girl who presented with fever and enlargement of both axillary lymph nodes. Both aerobic and anaerobic cultures of the lymph node aspirate were negative for microbial growth. Gram staining and Warthin-Starry silver staining did not reveal any organism. Purified DNA from the PCR-amplicon of the 16S-23S rRNA intergenic region was sequenced and showed 99.7% identity with the corresponding sequence of Bartonella henselae strain Houston-1. Our findings suggest that the internal transcribed spacer is a reliable region for PCR identification of Bartonella species. In patients with lymphadenitis, a history of contact with cats or dogs necessitates the use of diagnostic approaches that employ not only the conventional staining and culture but also molecular methods to detect B. henselae.
Animals
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Bartonella henselae/genetics/*isolation & purification
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Cat-Scratch Disease/complications/*diagnosis
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Cats
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Child
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Dogs
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Female
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Humans
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Lymphadenitis/complications
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*Polymerase Chain Reaction
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RNA, Ribosomal, 16S/*genetics
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RNA, Ribosomal, 23S/*genetics
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Republic of Korea
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Sequence Analysis, DNA