Objective To investigate the clinical value of CT scanning combined with ultrasound technique in diagnosis of malignant bone tumor.Methods 100 patients with malignant bone tumor were randomly divided into control group(50 cases with CT scan),and observation group (50 cases received CT scanning combined with ultrasound technology).The diagnostic accuracy rate,tumor bone formation and destruction of bone,periosteal reaction and soft tissue mass were compared between the two groups.All results were judged by the results of MR.Results Diagnostic accuracy rate of the observation group was 80.0％ (40/50),which was significantly higher than 64.0％(32/50) in the control group (x2 =4.65,P ＜ 0.05).In observation group,diagnosis of bone destruction in 60 cases,the tumor bone formation 10cases,83 cases of periosteal reaction and soft tissue mass in 40 cases,those in the control group were 40 cases,5 cases,62 cases,30 cases,the differences between the two groups were statistically significant(x2 =4.86,5.71,6.21,5.22,all P ＜ 0.05).Conclusion The combination of CT and ultrasound in the diagnosis of malignant bone tumors had high diagnostic accuracy rate,and could guide clinical treatment.

Objective:To detect the expression of chemokine receptor CCR5 in human invasive ductal breast carcinoma and explore the role of CCR5 in breast cancer.Methods: Thirty samples of invasive ductal breast carcinoma and 10 samples of breast fibroadenoma were analyzed separately by immunohistochemical staining for CCR5 expression. MIP1?,MIP1? and RANTES concentrations were measured by ELISA in the sera of patients. Results: The expression of CCR5 was detected in invasive ductal breast carcinoma (the positive rate was 53.3%) and was not detected in breast fibroadenoma. RANTES concentrations in the serum of breast carcinoma patients increased significantly (P

Objective To investigate the effect of CCAT1 on migration,invasion and epithelial-mesenchymal transition (EMT) in endometrial carcinoma (EC) cell.Methods Using quantitative PCR assay,level of CCAT1 was detected in EC tissues to find its association with the initiation and malignancy degree of EC.Wound heal assay and transwell invasion assay were performed to study the effect of CCAT1 on migration and invasion ability of EC cell,while qPCR and western blot assay were utilized to detect the levels of related genes.Results In EC tissues,level of CCAT1 was significantly upregulated (P ＜ 0.001) and was positively associated with the malignancy degree of EC.After CCAT1 knockout,shorter migration distance was found,fewer cell passed through the chambers,levels of metastasis-related genes (MMP2 and MMP9) and mesenchymal markers (Snail,Zeb1 and Twist1) were up-regulated,and epithelial markers (E-cadherin and ZO-1) were down-regulated.Conclusion CCAT1 was up-regulated in EC tissue and its expression level was positively associated with the malignancy degree of EC.CCAT1 knockdown inhibited the metastasis,invasion and epithelial-mesenchymal transition of EC cell.

Two highly conserved and with abundant quantity of lipoproteins in outer membrane of pathogenic species,but not in saprophytic species of leptospira ,LipL32 and LipL21,were selected to construct the fusion gene DNA vaccine pVAX1/LipL21-LipL32,and its ability to induce immune responses in BALB/c mice inoculated with this recombinant DNA vaccine was investigated in the present study.Expression of the fusion-protein LiPL21-LiPL32 was demonstrated in HEK293 cells following transfection with the fusion gene DNA vaccine and the immune responses induced after intramuscular inoculation with this DNA vaccine in BALB/c mice was then evaluated by microscopic agglutination test (MAT),meanwhile the ELISA assay was used to detect the cytokines induced.It was demonstrated that significant level of specific antibodies agglutinating antigens of Leptospira interrogans could be detected by MAT after DNA vaccine inoculation.The production of cytokines IL-10 and TNF-β in mice inoculated with DNA vaccine pVAX1/LipL21-LipL32 was significantly increased in comparison with that of the group inoculated with pVAX1 alone.These results indicate that the recombinant DNA vaccine pVAX1/LipL21-LipL32 may be of potential value to design and develop new generation of vaccines against leptospirosis.

Objective To evaluate the value of neurophysiological index ( NI) in evaluating the rate of progression of amyotrophic lateral sclerosis ( ALS ) . Methods Forty-eight patients with ALS were enrolled in Zhongda Hospital of Southeast University from January 2010 to August 2016, of whom 36 patients met the ALS definite diagnostic criteria , 12 patients met the ALS probable diagnostic criteria ( following study confirmed those patients met the ALS definite diagnostic criteria ) , including 8 bulbar-onset and 40 upper extremity onset forms of the disease .Fifty-four age-and sex-matched healthy subjects served as controls.After evaluated by the ALS Functional Rating Scale-revised ( ALSFRS-R) , all subjects underwent electrophysiological examination in the Electromyography Lab of the hospital .The rate of disease progression (ΔFS) =48-total ALSFRS-R score at initial visit/symptom duration (months).The relevance between the complex muscle action potential (CMAP), F frequency, distal motor latency (DML), NI and the ΔFS was investigated respectively.Results In ALS group, the ALSFRS-R score was 14.56 ±10.10, the duration from onset to diagnosis was (14.56 ±10.10) months, and theΔFS was 1.54 ±1.18 per month.The median nerve NI in ALS group was 0.60 ±0.76, in control group was 2.56 ±0.78, with statistically significant difference between two groups (t=-12.5, P<0.01).The ulnar nerve NI in ALS group was 0.70 ±0.55, in control group was 0.96 ±0.10, also with statistically significant difference between two groups (t=-0.31, P=0.003).The median nerve NI and ulnar nerve NI both were negatively correlated withΔFS (r=-0.63, P=0.000; r=-0.506, P=0.007).The ΔFS was exponentially based on median nerve NI (R2 =0.668, P<0.01).Conclusion NI is an objective electrophysiological index , which could be used to evaluate the rate of disease progression in ALS , and to evaluate the prognosis of the disease .

Objective:To investigate the value of growth differentiation factor-15 (GDF-15) and extravascular lung water index (EVLWI) in severity grading and prognosis prediction of patients with acute respiratory distress syndrome (ARDS).Methods:Patients with ARDS aged 18-75 years admitted to the department of respiratory intensive care unit (RICU) of Zhengzhou Central Hospital Affiliated to Zhengzhou University from January 2019 to February 2020 were enrolled. All patients were treated with conventional therapies such as mechanical ventilation, anti-infection, stabilization of water, electrolytes and acid-base environment, blood purification and nutritional support according to their conditions. Besides, the pulse-indicated continuous cardiac output (PiCCO) was performed after admission to the department, and EVLWI before treatment and at 24, 48 and 72 hours of treatment were recorded. Serum GDF-15 level was measured by enzyme linked immunosorbent assay (ELISA) during the same period. Patients were classified as mild, moderate, and severe degree according to the 2012 Berlin Definition of ARDS, and EVLWI and GDF-15 levels in patients with different disease levels before and after treatment were compared. In addition, the length of intensive care unit (ICU) stay, ICU mortality, and 28-day mortality of patients with different GDF-15 or EVLWI levels were analyzed comparatively, with the GDF-15 3 458 ng/L and EVLWI 15 mL/kg as the cut point.Results:A total of 82 patients with ARDS were enrolled, including 22 patients with mild ARDS, 28 patients with moderate ARDS, and 32 patients with severe ARDS. The GDF-15 and EVLWI levels in patients with moderate and severe ARDS at each time point before and after treatment were higher than those in patients with mild ARDS. Both GDF-15 and EVLWI levels in patients with severe ARDS were higher than those in the patients with moderate ARDS. The differences were statistically significant at all the time points except for the difference of GDF-15 levels at 24 hours after treatment (ng/L: 3 900.41±546.43 vs. 3 695.66±604.73, P > 0.05). [GDF-15 (ng/L): 3 786.11±441.45 vs. 3 106.83±605.09 before treatment, 3 895.48±558.96 vs. 3 333.29±559.66 at 48 hours, 3 397.33±539.56 vs. 3 047.53±499.57 at 72 hours; EVLWI (mL/kg): 19.06±1.91 vs. 14.31±1.50 before treatment, 18.56±2.23 vs. 13.26±1.69 at 24 hours, 17.23±1.76 vs. 12.45±1.36 at 48 hours, 15.47±1.81 vs. 11.13±2.19 at 72 hours, all P < 0.05]. According to the cut-off value, there were 23 patients with GDF-15 ≥ 3 458 ng/L and GDF-15 < 3 458 ng/L respectively and there were 23 patients with EVLWI ≥ 15 mL/kg and EVLWI < 15 mL/kg respectively. The length of ICU stay and 28-day mortality in patients with high GDF-15 were significantly higher than those in patients with low GDF-15 [length of ICU stay (days): 21.22±2.69 vs. 15.37±3.14, 28-day mortality: 56.5% vs. 21.7%, both P < 0.05]. The length of ICU stay and 28-day mortality in patients with high EVLWI were also significantly higher than those in patients with low EVLWI [length of ICU stay (days): 18.45±2.61 vs. 14.98±2.75, 28-day mortality: 47.8% vs. 17.4%, both P < 0.05]. Conclusion:To some extent, GDF-15 and EVLWI levels reflect the severity of patients with ARDS, and high GDF-15 and EVLWI levels are significantly associated with poor prognosis in patients with ARDS.

ObjectiveTo explore the correlations between the medicinal parts， habitats， tissue， components， and medicinal properties （natures， tastes， and effects） of 100 aquatic Chinese medicinal plants， thus providing evidence for the relationship between image and analogy and laying a theoretical foundation for the clinical use and development of aquatic Chinese medicinal plant resources. MethodThe Aquatic Plants of China， Flora of China， and related literature were searched， and a total of 100 aquatic Chinese medicinal plants included in the Chinese Pharmacopoeia （2020 edition）， Chinese Materia Medica， National Compilation of Chinese Herbal Medicines， Dictionary of Chinese Materia Medica， and local standards were screened out. The medicine-taxonomic status-life habit associations and nature-component-traditional Chinese medicine classification-pharmacological effect associations were analyzed respectively. ResultThe aquatic Chinese medicinal plants mostly had a cold nature， bitter， pungent， and sweet tastes， and tropism to the liver meridian. The whole plant or aerial part was mainly used for medicinal purposes， and these plants generally had well developed aerenchyma. These plants mainly contained flavonoids， alkaloids， and volatile oils and had the effects of clearing heat， detoxifying， and promoting urination. ConclusionThere are correlations between the medicinal parts， habitats， tissue， components， medicinal properties， and effects of aquatic Chinese medicinal plants， which provide reference for the development and utilization of aquatic Chinese medicinal plant resources.

Objective: To investigate the effect of PM2.5 exposure on the thyroid function in male SD rats. Methods: Adult Sprague-Dawley rats were divided into " filtered air" (FA) and " PM2.5" groups. These rats exposed to the 2 kinds of air for 12 weeks were killed and their plasma and thyroid tissues were taken for investigation. The morphological status and pathological changes of thyroid tissue were observed after H&E staining. The levels of triiodothyronine (T₃), free triiodothyronine (FT₃), thyroxine(T₄), free thyroxine (FT₄), anti-thyroglobulin antibody (TGAb), anti-thyroid peroxidase antibody (TPOAb), thyrotropin (TSH), tumor necrosis factor α (TNF-α), and interleukin-6 (IL-6) in rats were measured by ELISA. Results: The results showed that there was no significant difference in body weight between two groups after 12 weeks of exposure to either filtered air or PM2.5. No pathological changes and significant differences were observed in the thyroid tissues of two groups after HE staining. There were no significant differences in plasma levels of T₄, T₃, FT₃, TNF-α, and IL-6 between two groups. However, compared with rats exposed to filtered air, rats exposed to PM2.5 had significantly lower plasma FT₄[(3.81±1.23) vs (6.02±2.40)pmol/L, P<0.01] and higher TSH [(5.63±1.74) vs (4.29±1.37) mU/L, P<0.05] levels. Conclusion The exposure to PM2.5 for a period of 12 weeks may lead to the decrease of FT₄ and increase of TSH in rats.

ObjectiveTo investigate the correlation among the botanical characteristics， biological characteristics， chemical composition， and medicinal properties and efficacy of traditional Chinese medicines （TCM） from latex-containing plants， so as to strengthen the theory of "identifying symptoms for qualities" and provide a reference for the development and utilization of the latex-containing plant resources. MethodStatistics on the meridians for properties and tastes， efficacy， medicinal parts， family and genus， and chemical components of TCM from latex-containing plants were carried out. A total of 53 TCM from latex-containing plants included in the 2020 edition of the Chinese Pharmacopoeia were screened by mining the Chinese Botanical Journal， Chinese Materia Medica， Dictionary of Traditional Chinese Medicines， and related literature. In addition， their meridians for properties and tastes， medicinal parts， chemical components， and TCM classifications were summarized and statistically analyzed by using Excel 2013 and ChiPlot 2023.3.31 software. ResultIt was found that latex-containing plants were mainly distributed in one kingdom， one phylum， two classes， and 20 families， and most of the TCM from latex-containing plants belonged to Dicotyledonaceae under Angiosperms. In terms of properties and tastes， plain>cold>warm>cool>hot and bitter>pungent>sweet>sour>salty. In terms of meridians， liver>lung>kidney>spleen=large intestine=stomach>heart>bladder=gallbladder=small intestines. In terms of medicinal parts， roots （root， rhizomes， tuberous root， and root bark）>resin>seed>whole herb （whole herb and above-ground part）>stem （stem and branch）>fruit>leaf>flower=skin. In terms of research on chemical components， they were mostly glycosides. In terms of TCM classification， they were mostly medicines for activating blood circulation and removing blood stasis. ConclusionThe TCM from latex-containing plants is mainly plain， with a uniform warm and cold distribution. The tastes are mainly bitter and pungent， and the major meridians are the liver and lung. The roots and resins are mainly used as medicines. The components mostly contain glycosides， alkaloids， and volatile oils， and most of them are medicines for activating blood circulation and removing blood stasis， as well as for removing heat and toxins. There is a certain degree of correlation among the growth habits， medicinal parts， chemical components， and the properties， tastes， and efficacy of the TCM from latex-containing plants. It may provide a reference for resource development and utilization of TCM from latex-containing plants.

ObjectiveThe relevant laws among the biological characteristics， medicinal parts， growth environment， and medicinal properties and efficacy of traditional Chinese medicines （TCM） from fungi were excavated， so as to strengthen the theory of distinguishing symptoms for quality and provide a reference for the development and utilization of TCM from fungi. MethodThe medicinal parts， meridians for properties and tastes， heterotrophic mode， and efficacy of commonly used TCM from fungi were summarized. By consulting the Compendium of Materia Medica， Shennong Materia Medica， Flora of China， and literature， the TCM from fungi indexed in the 2020 edition of the Chinese Pharmacopoeia and some local pharmacopeias were checked. ResultA total of 28 common TCM from fungi were selected. Different TCMs from fungi have different meridians for properties and tastes， medicinal parts， habits， and growth environments. The relevant information was counted. Among the four properties， plain>cold>warm. Among the five tastes， sweet>bitter>light>pungent=salty. In terms of medicinal parts， fruiting body>sclerotia>complex>spermia=outer skin=other. In terms of meridians， lung>liver=heart>spleen=kidney>stomach. In terms of habits， parasitism>saprophysis>symbiosis=facultative parasitism=facultative saprophysis. ConclusionTCM from fungi are mainly parasitic and saprophytic， and the plain property and sweet taste the most. The meridians are mostly lung， heart， and liver. Nourishment and diuresis are the main efficacy. There is a certain correlation between the color， habit， medicinal parts， and growth environment of TCM from fungi and their properties， tastes， and efficacy， providing comprehensive literature reference and theoretical basis for their in-depth research， clinical use， and resource development.