Objective To investigate the clinical feature of Acinetobacter baumannii and to provide the basis for clinical rational use of anti-microbial agents.Methods ATB system was used to identify Acinetobacter baumanii, and antimicrobial resistance was determined by Kirby-Bauer method.The results were analyzed by Whonet 5.4 soft-ware.Results A total of the 358 strains Acinetobacter baumannii were isolated,91.62% were from sputum and throat swab.The main departments was ICU(52.23%);In 358 strains Acinetobacter baumannii,217 strains were multi-drug resistant strains(60.61%).The drug resistance to polymyxin B was the lowest 0% followed by minocy-cline 19.8% and cefoperazone/sulbactam 9.8%, the next was netilmicin 21.1% and meropenem 41.5%. Conclusion Acinetobacter baumannii shows multi-drug resistance, especially in ICU.Anti-microbial agents should be the rational use according to the results of drug susceptibility in order to reduce and control the incidence of noso-comial infections.

By analyzing "Diagnose and treatment of cholera in Shanghan Lun (Treatise on Febrile Diseases)", we found that such symptoms as serious vomiting and dysentery appeared in febrile diseases, which is similar to cholera. Therefore, Shanghan Lun named it "cholera illness". This name not only clearly manifested the characteristics of such gastrointestinal-febrile disease, but also emphasized the significance of differential diagnosis between cholera with "cholera illness" in febrile diseases.

Objective To investigate the effect of Xinhuang Pian on pancreatic apoptosis and the protein expression of Bcl-2 and c-Myc in rat with acute pancreatitis (AP). Methods 90 Wister rats were randomly divided into 3 groups, including control group, AP group and Xinhuang Pian treatment (XP) group.AP model was induced by pancreaticobiliary injection of 5% sodium taurocholate (1ml/kg weight). Rats in XP group were given Xinhuang Pian solution (480 mg/kg weight) lavage 30min after AP induction, The rats were sacrificed at 6, 12, 24 h after AP induction, and serum amylase levels were determed, pancreatic pathological change was observed; apoptosis was determined by TUNEL method. The determination of Bcl-2and c-Myc protein expression was performed by Western blot. Results 12 h after AP induction, serum amylase, pancreatic pathological scores, apoptotic indexes and the protein expression of Bcl-2 and c-Myc were (1927 ± 186) U/L, 0, 0.59 ± 0. 12, 0.406 ± 0. 112, 0. 185 ± 0. 046 in control group; (5611 ± 473) U/L,2.65 ± 0. 56, 3.80 ± 0. 91, 0. 282 ± 0.082, 0. 339 ± 0. 076 in AP group; and (4572 ± 400) U/L, 2.40 ± 0. 75,7.15 ± 0. 86, 0. 220 ± 0.068, 0. 302 ± 0. 090 in XP group. The levels of serum amylase, pancreatic pathological scores, apoptotic indexes and the protein expression of c-myc in AP and XP group were all significantly higher than those in control group (P < 0. 05), while the protein expression of Bcl-2 was significanfiy lower than that in control group ( P < 0.05 ). Compared with AP group, the levels of serum amylase, pancreatic pathological scores, protein expression of Bcl-2 were all significanfiy lower in XP group (P < 0. 05 ), but the apoptotic indexe was significantly higher (P < 0. 05 ), and there was no significat change of expression of c-Myc protein.Conclusions Xinhuang Pian may induce the pancreatic apoptosis through down-regulating Bcl-2 protein expression, and result in the alleviation of pancreatic inflammatory reaction.

Objective To investigate the influence of astragalus polysaccharide administration on the the expression of pancreatic Kir6.2 in diabetes rats.Methods Wistar rats were induced diabetes mellitus,then divided into model group,control group,low dose group,and high dose group randomly.After OGTT of them were observed,determination of the Kir6.2 mRNA and protein expression levels in pancreas was performed by RT-PCR and Western blotting.Results The expression of Kir6.2 in model group decreased significantly,and the expressions of Kir6.2 in low dose and hish dose groups were higher than the model group (P<0.05).Conclusion Astragalus polysaccharide displayed up-regulation to the expression of pancreatic Kir6.2.which may be a mechanism on reducing blood glucose.

Objective To compare the diagnostic efficacies of narrow-band imaging (NBI) in distinguishing neoplastic from non-neoplastic colorectal lesions with routine endoscopy and with magnifying endoscopy. Methods Patients with colorectal lesions detected by NBI from September 2008 to February 2010 were enrolled in the study. These lesions were classified by pit pattern and capillary pattern, which was then assessed by reference to histopathology. Results A total of 100 patients with colorectal lesions were enrolled, and the lesions were observed by NBI with ordinary endoscopy (n =64) and NBI with magnifying endoscopy (n =36), respectively, and 7 cases (5 in NBI with ordinary endoscopy and 2 in NBI with magnifying endoscopy) which did not meet the diagnostic criteria were excluded. The overall diagnostic accuracy of NBI endoscopy in distinguishing neoplastic from non-neoplastic colorectal lesions was 91.4% ( 85/93 ), in which NBI with ordinary endoscopy and magnifying endoscopy was 89. 8% (53/59) and 94. 1% (32/34),respectively, with both significantly higher than that of conventional colonoscopy reported in the literature (79. 1% ) (P ＜ 0. 05 ). However, no significant difference was detected between 2 methods ( P ＞ 0. 05 ).Conclusion Similar with NBI magnifying endoscopy, NBI endoscopy without high magnification may also be useful to distinguish neoplastic from non-neoplastic colorectal lesions.

Objective To investigate whether and what staining techniques are applied to the ultrathin sheet plastination slice and whether the stained specimen is of autofluorescences .Methods A cadaveric hand block was plastinated and then sectioned as a series of 300-400μm thick transverse sections .A total of 56 slices in total .Alternative sections were stained with hematoxylin -eosin staining ( HE) , Verhoeff -Van Gieson staining ( VVG) or methylene blue and azureⅡstaining(MA).The stained slices were examined under a light microscope and a confocal microscope .Results The plastinated slices were stained with the three staining methods .HE staining revealed the muscle and connective tissues were red or violet , bone was violet or blue;VVG staining showed the elastic fibers was black , the collagen was red , and other tissues were yellow .MA staining showed the tendon was violet , the bone was pink , cartilage was violet , and other tissues were purple.However, the intracellular structures appeared not very well stained .The collagen, elastin and muscular structures in the stained slices were observed under a confocal microscope .Conclusion The commonly used histology staining methods can be used to stain the ultrathin sheet plastination slices .The staining provides a better observation of various tissues in the slice than the unstained slice .After staining, those autofluorescent structures in the plastinated slice are detectable under a confocal microscope .

Objective To determine the feasibility and accuracy of velocity propagation within main pulmonary artery(VP)from color M-mode Doppler imaging using custom software on a personal computer for noninvasive estimation of PVR.Methods Color M-mode imaging of pulmonary flow was obtained and then transferred to computer,the velocity propagation of pulmonary flow was automatically obtained.Comparative studies among Doppler echocardiography,personal computer and cardiac catheterization for predicting PVR had been done in 20 children with congenital heart disease and 20 normal children.Results Velocity propagations of children with congenital heart disease were significant lower than those of normal children obtained by color Mmode echocardiography[(38.38±18.89)cm/s VS(80.34±15.65)cms,P＜0.01),and correlated well with invasive PVR measurements(r=-0.69,P＜0.01).The correlation and repeatability of VP obtained by the custom software were better than VP obtained by Doppler echocardiography(r=-0.78,P＜0.01).A VP cutoff value obtained by the custom software of 35.910 had a sensitivity of 92.9% and a specificity of 100% to within pulmonary artery obtained by color M-mode echocardiography using custom software on a personal computer.

Objective To investigate the effect of interfering Hiwi gene on the apoptosis of MDA-MB-231 cells. Methods The mRNA and protein expression of Hiwi mRNA and its target protein were analyzed by qRT-PCR and Western Blot after transfection. MDA-MB-231 cells were divided into 6 groups according to the experimental design. Interference effects were screened as siRNA interference group (Hiwi10330 group), and then divided into 3 groups according to the experimental design: interference group, negative control group/NC, blank control group/Blank. The cell apoptosis rate was detected by flow cytometry after transfection. Results The expression of mRNA in the interference group was significantly lower than that in the siRNA group (P < 0.05), the expression of target protein of Hiwi gene was also significantly inhibited (P < 0.05). The apoptosis rate of MDA-MB-231 cells was significantly higher than that of NC and Blank groups (P<0.05). Conclusion The apoptosis rate of breast cancer cells MDA-MB-231 was significantly increased after siRNA targeting hiwi gene silencing.

Objective To analyze the cancer mortality and epidemiological characteristics of digestive system cancer in Xianyou County, Fujian Province, and to provide a scientific evidence for formulating preventive measures for digestive system cancer. Methods Excel and SPSS software were used to collect the monitoring data of digestive system cancer death in Xianyou County from 2014 to 2018, and then analyzed the mortality, the standardized rate (according to the Chinese 2000 population census) and the cause of death. Results From 2014 to 2018, the deaths of malignant tumors in Xianyou County accounted for 35.00% of all deaths. Among them, the four common digestive system malignant tumors (gastric cancer, esophageal cancer, liver cancer and colorectal cancer) accounted for 62.44% of malignant tumors. Gastric cancer ranked first and accounted for 27.36%. Average annual rate of crude mortality and standardized mortality of malignant tumor in the digestive system were 108.93/10⁵ and 89.34/10⁵, respectively, and the ratio of male to female was 2.27 to 1. Significant differences were found between town and village, as the terrain gets lower, the death rate goes down gradually. Compared with the data of 2011-2012, the standardized death rate of gastric cancer, esophageal cancer and liver cancer were reduced by 29.71%, 23.92% and 12.06%, respectively, while the rate of colorectal cancer was increased by 28.80% and the average annual growth rate was 7.83%. Conclusion Although the death rates of gastric cancer, esophageal cancer and liver cancer have decreased significantly, the malignant tumor of the digestive system is still the main disease that causes the death of residents in Xianyou County, Putian City. Therefore, it is necessary to strengthen the education of health in order to achieve the early discovery, diagnosis and treatment.