1.SELECTION OF THE ACID AND HEAT-RESISTANT PULLULANASE-PRODUCING STRAIN AND ITS FERMENTATION CONDITIONS
Baoying TANG ; Xiaohui ZHU ; Jia LIU
Microbiology 2001;(1):39-43
A strain producing pullulanase was isolated from soil and mutated by UV and EMS with the increase of pullulanase activity from 1.6 to 5.4 u/mL. The fermentation conditions and medium were studied and optimized. The enzyme activity can reach 8.8 u/mL under the optimal fermentation conditions and medium. The optimal pH and temperature for enzyme are 4.6 and 75℃ respectively. It shows a high stabiliby during pH4.0～8.0 at 55℃.
2.SELECTION OF THE ACID AND HEAT-RESISTANT PULLULANASE-PRODUCING STRAIN AND ITS FERMENTATION CONDITIONS
Baoying TANG ; Xiaohui ZHU ; Jia LIU ;
Microbiology 1992;0(01):-
A strain producing pullulanase was isolated from soil and mutated by UV and EMS with the increase of pullulanase activity from 1.6 to 5.4 u/mL. The fermentation conditions and medium were studied and optimized. The enzyme activity can reach 8.8 u/mL under the optimal fermentation conditions and medium. The optimal pH and temperature for enzyme are 4.6 and 75℃ respectively. It shows a high stabiliby during pH4.0～8.0 at 55℃.
4.Study on chemical constituents of Scutelliaria regeliana.
Jinlan WANG ; Baoying ZHAO ; Hongmei XU ; Ming ZHAO ; Wanxia TANG ; Shujun ZHANG
China Journal of Chinese Materia Medica 2011;36(23):3270-3275
OBJECTIVETo study the chemical constituents in ethyl acetate fraction from the root of Scutelliaria regeliana.
METHODThe compounds were isolated by silica gel column chromatography and HPLC, and their structures were elucidated by means of spectral analyses.
RESULT23 compounds were isolated and identified.
CONCLUSIONCompound 1 is new, named as scutellariae flavonol, and the others were isolated from S. regeliana for the first time.
Chromatography, Gel ; Chromatography, High Pressure Liquid ; Plant Extracts ; chemistry ; isolation & purification ; Scutellaria ; chemistry
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