Objective To investigate the clinical feature of Acinetobacter baumannii and to provide the basis for clinical rational use of anti-microbial agents.Methods ATB system was used to identify Acinetobacter baumanii, and antimicrobial resistance was determined by Kirby-Bauer method.The results were analyzed by Whonet 5.4 soft-ware.Results A total of the 358 strains Acinetobacter baumannii were isolated,91.62% were from sputum and throat swab.The main departments was ICU(52.23%);In 358 strains Acinetobacter baumannii,217 strains were multi-drug resistant strains(60.61%).The drug resistance to polymyxin B was the lowest 0% followed by minocy-cline 19.8% and cefoperazone/sulbactam 9.8%, the next was netilmicin 21.1% and meropenem 41.5%. Conclusion Acinetobacter baumannii shows multi-drug resistance, especially in ICU.Anti-microbial agents should be the rational use according to the results of drug susceptibility in order to reduce and control the incidence of noso-comial infections.

OBJECTIVETo establish a method for determining 2, 4-D butylate in serum by gas chromatography (GC)and to provide a basis for the diagnosis and treatment of clinical poisoning.

METHODSSerum 2, 4-D butylate level was determined by the following steps: mixing serum (0.5 ml)with trichloromethane (2.0 ml), adequately shaking for extraction, standing for 5 min, centrifuging at 4 000 rpm for 10 min, blow-drying the trichloromethane layer with nitrogen, adding ethanol (50 µl)to a certain volume, adding the sample (1.0 µl), and performing GC with a hydrogen flame ionization detector.

RESULTSSerum 2, 4-D butylate level showed a linear relationship within 5∼40 µg/ml, with a regression equation of y = 1 831.6.4x-899.4 (r = 0.999 2); the minimum detectable concentration was 1.0 µg/ml. The recovery rate was 88.7%∼103.0% (relative standard deviation (RSD) 3.8%∼5.0%). The intra-day RSD and inter-day RSD were 3.87-4.92% and 3.33%∼5.34%, respectively.

CONCLUSIONThis determination method is simple, efficient, and accurate and provides a good means for rapid diagnosis and treatment of 2, 4-D butylate poisoning.

Chromatography, Gas ; methods ; Humans ; Serum ; chemistry ; Thiocarbamates ; blood