Objective:To investigate the effects of PHT on the differentiation of rat bone marrow mesenchymal stem cells(BMSCs) into vascular endothelial cells(VECs).Methods:rBMSCs were cultured in indirect coculture system with VECs and independent culture system in the presence of phenytoin at 0,20 and 40 μg/ml respectively.After 1 4 d culture the mRNA expression levels of the intercellular adhesion molecule-1 (ICAM-1 ),vascular cell adhesion molecules-1 (VCAM-1 ),vascular endothelial growth factor re-ceptor -2(KDR)and Runt-related transcription factor 2 (RUNX2)were detected by real-time PCR.Results:The expression levels of ICAM-1 ,KDR and RUNX2 were upregulated in rBMSCs by PHT treatment.The expression levels of ICAM-1 ,KDR and RUNX2 in the co-culture groups were higher than those in the independent culture groups of rBMSCs treated by PHT at the same concentra-tion.The expression level of VCAM-1 in co-culture groups was lower than that in the independent culture groups.Conclusion:PHT may promote rBMSCs differentiation into rVECs in the co-culture system.

砄bjecat:To study the correlatiom between stress and structure of one piece spiral implant.Methods: The morphology of alveolar bone was selected by CT around the first molar of mandible and around the incisor of maxilla of a healthy man in the position of normal occlusion. Cortical bone with the thickness of 1～2 mm was used to make the alveolar bone model. The one piece spiral implants with root, in the shape of cylinder, length of 10,12, 13 and 14 mm, corona height of 4 and 9 mm,diameter of 3.6,3.7, 3.8,3.9 and 4.0 mm were prepared respectively. The implants were put into the alveolar bone models and a load of 200 N was applied vertically, or 45 ℃ obliquely or 45℃ non center obliquely. The stress on the implants was measured and studied with 3D FEM. Results:The stress on the implants was inversely correlated to the length and diameter in the place of front teeth and molars except non centeraly obliquely loading to molar. The greatest stress was found around the neck of the implants. The distribution and intensity of the stress were related to the way of loading and the elastic modulus of bone. Conclusion:The length and diameter of one piece implant are the factors correlated to the stress on the implant and surrounding bone.

Objective To study the expression of human telomerase reverse transcriptase (hTERT) and P16 protein and their relation with telomerase activity in the carcinogenesis of acute leukemia. Methods Telomerase activity and hTERTmRNA were detected in 40 patients with acute leukemia and 20 normal controls by TRAP and RT-PCR, respectively; P16 protein was examined by immunocytochemical method. Results The positive rates of telomerase activity and hTERTmRNA were 75% and 80% in 40 cases of acute leukemia, respectively, whereas neither hTERTmRNA nor telomerase activity was detected in normal controls. There was a significant correlation between the expression of hTERTmRNA and telomerase activity (r= 0.65, P

Objective To conduct the global assessment of methylated DNA status of CpG islands. Methods The modified AIMS (amplification of inter-methylated sites) technology was adopted, which mainly included 3 groups of isoschizomers (methylation-sensitive and methylation-insensitive restricted enzymes). The comparison analysis of methylome among 3 concentrations (6%, 8%, 10%) of polyacrylamide with 8M urea denaturing sequencing gel and the digestion effect among 3 groups of isoschizomers were carried out. The results of personal molecular imager FX system and autoradiography were also compared. Results Genome-wide detection of methylome of CpG islands might be the technical basis for the subsequent analysis. The resolving power and scope bands in 6% polyacrylamide denaturing sequencing gel were superior to those in gels with the other two concentrations. 3 groups of isoschizomers could meet the need of genome-wide detection of methylome of CpG islands. Personal molecular imager FX system and autoradiography could be used together, and they compensated each other. Conclusions The optimized AIMS technology is a kind of high-throughput method to analyze methylome, which is simple, specific, and easy to handle. The 3 groups of isoschizomers can cover most of CpG islands for genome-wide detection and get the ideal results. Only a small quantity of genome DNA is enough to meet the need of clinical detection.

?Objective: To study the stress distribution of one segment type mono dowel implant with various length and diameter in alveolar bone. Methods: The thickness of cortical bone is 1～2 mm, implant was in close contact with alveolar bone directly. Implants with root length of 10,12,13 and 14 mm, cast diameter of 3.6, 3.7, 3.8, 3.9 and 4.0 mm, corona tall 4 and 9 mm were put into a bone model with alveolar bone. 200 N force was loaded in vertical, 45? oblique and 45? oblique of non center. CT and 3D FEM were used to study the stress distribution and selected the morphology of alveolar bone from first molar of lower jaw and an incisor of maxillary. Results: There was an area of stress in the boundary of the cortical and cancellous bony tissue,horizontal hole. The main region of stress was at the cancellous. Thickness and shape of alveolar bone could affect the distribution of stress. The maximal Von Mises Stress was always in the boundary of cortical and bone that contacted with implant. Higher elastic modulus of bone around the implant had higher stress in cortical stress . Conclusion: The stress of cortical and cancellous bone can be depressed to rise elastic modulus of implant.

Objective To develop a capacity-controlled ventriculoperitoneal shunt device to relieve the symptoms of hydrocephalus patient.Methods The device was composed of head-end shunt tube,middle reservoir and celiac shunt tube.The ends of the reservoir were equipped with one-way valve to drive cerebrospinal fluid to celiac shunt tube in case of the pressure on the reservoir.The capacity-controlled drainage of cerebrospinal fluid was implemented through the times of the pressure.Results The device drained cerebrospinal fluid to relieve the symptoms of hydrocephalus patient,and the complications due to over-or under-drainage were eliminated.Conclusion The device gains advantages in design and easy operation,and is an effective and safe shunt choice for types of hydrocephalus patients.

This paper briefly introduces the research background, combined with the practical of project, analysis the feature of the construction of information collection system of TCM clinics, and puts forward how to use the method of project management in the construction of information collection system of TCM clinics. By exploring the Project Phasing, Work Breakdown Structure, Project Responsibility Matrix, and Project management standard system, it hopes to strengthen the awareness of project management in the construction of information collection system of TCM clinics, and to guide the project management and the system construction.

To develop a patient-reported outcome instrument for chronic gastrointestinal diseases and to select items by analyzing the data from clinical investigation.

ABSTRACT:Objective To investigate the effects of phenytoin (PHT)on the secretion of vascular endothelial growth factor (VEGF)and stem cell factor (SCF)based on the establishment of indirect co-culture system of rat bone marrow mesenchymal stem cells (BMSCs)and vascular endothelial cells (VECs).Methods Indirect co-culture model of rat BMSCs and VECs was established.Experimental groups:indirect co-culture groups (PHT concentrations were 0,20 and 40 μg/mL);the control group:BMSCs culture group and VECs culture group (PHT concentrations were 0,20 and 40μg/mL).The contents of VEGF and SCF in the culture supernatant were measured using double antibody sandwich ABC-ELISA method on cultivation days 2,4,6.Results ELISA assay of the rBMSCs and rVECs in indirect co-culture supernatants,collected on culture days 2,4 and 6 showed that:① VEGF:On culture day 2,VEGF level in the co-culture groups was significantly higher than those in BMSCs group (P <0.05)and rVECs group (P <0.001).As culture time prolonged and PHT concentration increased to 20 μg/mL and 40 μg/mL,VEGF level increased too (P <0.001,P <0.05 ).② SCF testing results showed that the secretion of SCF in co-culture groups was higher than that in the control groups.When PHT was 20 μg/mL,the secretion of
SCF increased as the incubation time increased;but as the incubation time increased, PHT concentration of 40 μg/mL made SCF content decrease.Each group did not significantly differ (P > 0.05 ).Conclusion PHT promotes the secretion of VEGF and may reduce the secretion of SCF.

In bone marrow transplantation (BMT), cytomegalovirus (CMV) interstitial pneumonitis (IP) is one of the most dangerous complications, which has been the first important cause to lead the failure of BMT. At present, there is no effective and specific therapy for CMV-IP, therefore how to prevent CMV infection effectively is a top task. From 1991 to 1996, we used comprehensive steps to prevent CMV-IP in BMT, and none of 14 patients developed CMV-IP. The preventing results that we achieved by using the steps were quite satisfied.