Acupuncture Therapy ; psychology ; standards ; Biomedical Research ; Humans ; Knowledge ; Moxibustion ; psychology ; standards ; Thinking ; Treatment Outcome

Carboxysomes are extremely efficient microcompartments committed to CO2 fixation due to tailored CO2-concentrating mechanism (CCM). In cyanobacteria and some chemoautotrophs, carboxysomes as organelle-like microbodies encapsulate ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and carbonic anhydrase (CA). Together with active inorganic carbon uptake transporters, carboxysomes accumulate HCO3(-) in the cytoplasm, leading to high efficiency of carbon fixation. Based on the elucidation of structures and functionalities, heterologous production of carboxysomes has been achieved so far. In fact, the genes encoding either vacant carboxysome shell or only interior components have been characterized. This review summarizes the discovery along with types, showcases molecular structures and roles of carboxysomes in CCM, and presents their broad applications in metabolic engineering.
Biological Transport ; Carbon ; metabolism ; Carbon Cycle ; Carbon Dioxide ; metabolism ; Cyanobacteria ; metabolism ; Metabolic Engineering

Objective:To investigate the effects of PHT on the differentiation of rat bone marrow mesenchymal stem cells(BMSCs) into vascular endothelial cells(VECs).Methods:rBMSCs were cultured in indirect coculture system with VECs and independent culture system in the presence of phenytoin at 0,20 and 40 μg/ml respectively.After 1 4 d culture the mRNA expression levels of the intercellular adhesion molecule-1 (ICAM-1 ),vascular cell adhesion molecules-1 (VCAM-1 ),vascular endothelial growth factor re-ceptor -2(KDR)and Runt-related transcription factor 2 (RUNX2)were detected by real-time PCR.Results:The expression levels of ICAM-1 ,KDR and RUNX2 were upregulated in rBMSCs by PHT treatment.The expression levels of ICAM-1 ,KDR and RUNX2 in the co-culture groups were higher than those in the independent culture groups of rBMSCs treated by PHT at the same concentra-tion.The expression level of VCAM-1 in co-culture groups was lower than that in the independent culture groups.Conclusion:PHT may promote rBMSCs differentiation into rVECs in the co-culture system.

Objective To investigate the mechanisms of plasma exchange(PE) in the treatment of chronic severe hepatitis. Methods Twenty five patients with chronic severe hepatitis were divided into two groups: treatment group and control group. The concentration of ammonia, endotoxin, TNF ?, TGF ? 1, HGF and amino acid spectrum were detected before and after therapy, respectively .Moreover, ammonia and endotoxin levels were detected before and after every treatment with ALSS. Results Compared with the control group ,there were significant decreases in the concentration of ammonia, endotoxin, TNF ?,TGF ? 1 in the treatment group( P

Objective Study the common symptoms of sub-healthy people. Methods Study the 59 symptoms of the 1 828 cases of sub-healthy people that the study of DME Research on TCM Syndrome of Sub-health State had collected. Select the symptoms by analysis of percentage, mean, correlation and discriminance. Finally, find out the symptomatic characters of the sub-healthy people by the data mining method of decision tree. Result There were 26 variables selected from the 56 symptoms. The result of decision tree showed that low quality of sleep, fatigue, dry in throat, acid and distend in eyes, ache, emptiness in heart had greater relations with sub-health. Conclusion Low quality of sleep, fatigue, dry in throat, acid and distend in eyes, ache, emptiness in heart are the symptoms that have greater relations with sub-health.

Acupuncture Therapy ; Aged ; Drugs, Chinese Herbal ; administration & dosage ; adverse effects ; Eye ; innervation ; Female ; Herpes Zoster ; drug therapy ; physiopathology ; therapy ; Humans ; Panax ; adverse effects ; chemistry ; Trigeminal Nerve ; drug effects ; physiopathology

Angiosteosis is associated with increased vascular stiffness and leads to clinical manifestations such as high blood pressure and heart failure,which increases the risk of cardiovascular morbidity and mortality.Angiosteosis is considered to be ectopic deposits of calcium phosphate crystallization in the form of hydroxyapatite in cardiovascular tissue.However,the mechanism of angiosteosis is not yet clear.The pathophysiologic process of angiosteosis is similar to normal bone mineralization.Matrix vesicles (MV) play an important role in bone mineralization.Researches confirmed the existence of MV in the calcified arteries under the microscope.Angiosteosis is widely found in clinical diseases,so the studies of the mechanism of angiosteosis and the role of MV in angiosteosis are of great significance.

Successful implementation of TCM scientific research projects is the main motive force of the development of traditional Chinese medicine. With the establishment of public welfare industry research in 2006, TCM scientific research projects have obtained increasing investment funds from the central budget. Therefore, how to organize TCM research projects becomes a new task for department of scientific research management. Through years of work experience in scientific research management, the authors made an analysis of the special project management paradigm in Chinese medicine industry from the basic situation of TCM industry, investment, organization and management, and appraisal mechanism.

IgG functionalized Mn doped ZnS quantum dots ( IgG-QDs ) were fabricated via the amide condensation between the carboxy groups on the surface of Mn doped ZnS quantum dots and the amino groups on the surface of IgG. The IgG antibody functionalized Au nanoparticals were synthesized via the electrostatic interaction between the Au nanoparticals and IgG antibody. Based on the fluorescence resonance energy transfer effect between the IgG-QDs and the IgG antibody functionalized Au nanoparticals, the IgG-QDs could be applied for low background phosphorescent sensing of IgG with the linear range of 1-10 μg/mL and the limit of detection of 0. 45 μg/mL. The proposed method was successfully applied to detect IgG in human serum without the disturbance of the background fluorescence and scattered light.

Objective To study the expression of human telomerase reverse transcriptase (hTERT) and P16 protein and their relation with telomerase activity in the carcinogenesis of acute leukemia. Methods Telomerase activity and hTERTmRNA were detected in 40 patients with acute leukemia and 20 normal controls by TRAP and RT-PCR, respectively; P16 protein was examined by immunocytochemical method. Results The positive rates of telomerase activity and hTERTmRNA were 75% and 80% in 40 cases of acute leukemia, respectively, whereas neither hTERTmRNA nor telomerase activity was detected in normal controls. There was a significant correlation between the expression of hTERTmRNA and telomerase activity (r= 0.65, P