Objective: To study the effect of palate repair operation on middle ear function. Methods: Cleft palate was repaired with double-opposing Z-plasty(DOZ) in 20 cases and with routin method (RM ) in 23 cases. Secretory otitismedia (SOM), high negative pressure (HNP), stapedial reflex(SR) and low acoustic compliance (LAC) were determined 3d before and 6 months after operation respectively. Results: Of the total 86 ears, before operation the persentages of SOM, HNP, SR and LAC were 61. 63, 75. 58, 29. 07 and 65. 15; after operation those were 31.39, 43.02, 66.28 and 33.72, respectively. Of the 40 ears of the cases treated with DOZ the persentages of those were 20.00, 27.50, 72.50 and 25.00) while of the 46 ears of the cases treated with RM those were 41.30, 56.52, 60.87 and 41.30 respectivellly. Conclusion: Middle ear function can be improved by palate repairs surgery, better results may be achieved with double opposing Z-plasty technique.

Objective :To develop an agent that is more active against receptor-bearing target cells without increasing the toxic effect on non-target cells. Methods :By the use of molecular biology techniques,we designed and constructed a fusion protein 5＇IL6-TNF△ by connecting the human interleukin-6 (hIL-6) gene and a human tumor necrosis factor α derivative (TNF△) gene througha synthetic linker sequence followed by subsequent expression in E. Coli. Results: In cytotoxicity assay with myeloma cell line U266, the normal type of 5＇ IL6-TNF△ showed an antitumor activity 3 times higher than that of TNF△;and the antitumor activity of 5＇IL6-TNF△ blocked by IL-6Rwas only 1/30 of that of normal type of 5＇ IL6-TNF△. Meanwhile,the 5＇IL6-TNF△ blocked by an ti-TNF antibody did not show any cytotoxicity to U266 cells. In activity assay with L929 cells ,the toxic effect of the fusion protein was found 1/22 of that of TNF△. Conclusion: The 5＇IL6-TNF△fusion protein might be a useful cytotoxic agent in cancer treatment.

Objective To investigate the chemical constituents of marine sponge A gelas mauritiana collected from the South China Sea .Methods The n-butanol extract of marine sponge A gelas mauritiana was separated and purified by repeated column chromatography on silica gel ,Sephadex LH-20 ,and reversed-phase high-performance liquid chromatography (RP-HPLC) .The chemical structures of those obtained compounds were determined on the basis of spectroscopic analysis and com-parison with reported data .The tumor cell growth inhibitory activity of these compounds towards human lung carcinoma cells A549 was tested .Results Eight compounds were isolated ,including agelasine A (1) ,agelasine B (2) ,epi-agelasine C (3) , (-)agelasine D (4) ,agelasine E (5) ,agelasine F (6) ,(-)ageloxime D (7) and aurantiamide acetate (8) .These compounds dis-played different level of tumor cell growth inhibitory activity towards cell A 549 in vitro .Compounds 1-3 showed significant ac-tivity towards cell A549 ,being similar to that of the positive control of adriamycin .Conclusion Compounds 1 ,2 ,4 ,5 ,6 ,8 were isolated for the first time from the sponge A gelas mauritiana .Cell A549 was selected for the first time for the activity evalua-tion of compounds 1~7 .Significant inhibition activity of compounds 2、3 may hold as a basis for further research .

Linear ubiquitination is an important post-translational modification that has been discovered in recent years. The linear ubiquitin chain is formed by the linkage of glycine residue of one ubiquitin protein to the methionine residue of another ubiquitin. This process is regulated by the linear ubiquitin chain assembly complex (LUBAC) and the OTU deubiquitinase with linear linkage specificity (OTULIN). Linear ubiquitination is involved in various biological processes, including immune response, inflammation, and cell apoptosis. Recent studies have shown that linear ubiquitination is closely related to the occurrence, development, and drug resistance of tumors by affecting signaling pathways such as NF-κB and Wnt/β-catenin. The research progress on the function of LUBAC and OTULIN in tumors was reviewed in this paper.

Liver cancer is the second leading cause of cancer-related deaths worldwide, with hepatocellular carcinoma (HCC) being the most common form of primary liver cancer. Glypican-3 (GPC3) is a cell membrane proteoglycan which is rarely expressed in normal adult tissues but is specifically upregulated in HCC, which makes GPC3 a reliable target for the diagnosis and treatment of HCC. The role of GPC3 in the regulation of cancer development through Wnt, YAP, hedgehog and other signaling pathways were reviewed in this article. GPC3-targeted therapies, such as monoclonal antibodies, bispecific antibodies, tumor vaccines, immunotoxins, CAR-T cells, and photosensitizer therapy were also summarized. These treatment methods offered promising approaches for HCC treatment and future treatment strategies for HCC based on GPC3 were prospected in this paper.