The strain of TB1 from the original stock which harboring a recombinant plasmid pMal-2X including apc gene from cyanophyceae had been subcultured serialyto 100~(th) generation in Amp~(+) plate. The growth characteristics and the morphology of the cultures did not show differences among the generations. The chromatogram of the plasmids in different generations digested by EcoRⅠ restriction enzymes were same, as well as DNA sequences showed no difference among generations. In addition to that, origin strain and 10~(th), 20~(th), 50~(th) and 100~(th) generation strain had a similar rAPC expression level. The immunology activity of the rAPC and SDS-PAGE of the proteins expressed in different generations were also same. From the above results, it is indicated that recombinant stain P1 has a high hereditable stability.

Objective:To find out the coefficients of garlic oil by Supercritical Fluid Extraction (SFE). Methods: Cutting, squeezing fermentation and fermentation and ethanol extraction of garlic were respectively as pretreatments and then the use of supercritical carbon dioxide as an extraction and given garlic oil was analysed by means of IR. Result: Garlic oil can be directly extracted by SFE and its best operation condition was under the pressure of 15 MPa and at 40 ?C extractability of garlic oil was up to 44.57% . Conclusion: Fermentation and ethanol extraction method is a best one for garlic oil obtained.

This article is mainly about the application status and current problems of multimedia technology in experiment teaching of human parasitology. It also discusses how to improve the application of multimedia technology in experiment teaching of human parasitology. Several aspects were discussed, such as discussion about cases, construction and application of multimedia resource database and abundant living teaching.