Objective To investigate the role of vascular endothelial growth factor D(VEGF-D) and vascular endothelial growth factor receptor 3(VEGFR-3) in lymphangiogenesis and lymphatic metastasis of colon carcinoma by observing the expression of VEGF-D and VEGFR-3,and calculated lymphatic vessel density(LMVD) in colon carcinoma. Methods The expressions of VEGF-D and VEGFR-3 were observed by SP immunohistochemistry in 55 colon carcinoma samples of different stages and differentiation degrees.LMVD in colon carcinoma was calculated by using Podoplanin as the specific marker of lymphatic endothelium. Results The positive expression of VEGF-D in colon carcinoma(54.5%) was higher than that in peritumoral tissues(P

Objective To study the levels of interleukin-6 (IL-6) and C-reactive protein(CRP) in umbilical cord serum of the newborns with premature rupture of membrane(PROM)and to explore the value of IL-6 and CRP in the diagnosis of early onset neonatal sepsis (EONS).Method A total of 187 term newborns with PROM ＞ 12 h who were born normal vaginally in our Hospital from April 2015 to December 2015 were enrolled in this study as the PROM group and another 50 term infants without PROM and infection as the control group.The levels of IL-6 and CRP in umbilical cord serum were quantified by ELISA,the results of which were compared between groups.Receiver operating characteristics (ROC) curves were drawn to find out the cut-off value of IL-6 and CRP for the diagnosis of EONS.Result The levels of IL-6 and CRP in umbilical cord serum in the PROM group were significantly higher than those in the control group [IL-6 20.3 (9.5,35.8) pg/ml vs.9.3 (6.9,27.5) pg/ml,CRP 0.42 (0.25,0.78) mg/L vs.0.33 (0.18,0.45) mg/L,P ＜ 0.05].The levels of IL-6 and CRP in the newborns whose mother had chorioamnionitis were significantly higher than those in the newborns whose mother was without chorioamnionitis [IL-6 62.5 (35.2,92.7) pg/ml vs.10.8 (9.3,33.4) pg/ml,CRP 0.86 (0.44,1.95) mg/L vs.0.35 (0.20,0.62) mg/L,P ＜0.05].The levels of IL-6 and CRP in the infants with PROM≥18 h was significantly higher than those in the infants with PROM ＜ 18 h [IL-6 32.1 (9.9,42.2) pg/ml vs.10.7 (9.2,32.6) pg/ml,CRP 0.44(0.29,0.86) mg/L vs.0.35 (0.23,0.61) mg/L,P ＜ 0.05].The levels of IL-6 and CRP in the neonates with EONS was significantly higher than those in the neonates without EONS [IL-6 92.0 (58.3,161.0) pg/ml vs.20.0(9.4,35.2)pg/ml,CRP 1.94(0.47,2.73) mg/L vs.0.38(0.24,0.67) mg/L,P ＜ 0.05].ROC curve analysis showed that the cut-off value of IL-6 and CRP for the diagnosis of EONS were 81.lpg/ml (sensitivity 76.5％,specificity 90.6％) and 1.88mg/L (sensitivity 64.7％,specificity 89.4％).With the combination of IL-6 and CRP levels,the sensitivity was 88.2％ and the specificity was 84.1％ for the diagnosis of EONS.Conclusion To measure the IL-6 and CRP levels in umbilical cord serum is helpful for the early diagnosis of EONS,and the combined detection of the 2 items may improve the sensitivity of diagnosis.

Objective To know the lavatory improvement in the rural areas in Shandong Province in 2005 and to present the data for the decision of the lavatory improvement in countryside in the future. Methods In 2005, 164 villages in 45 counties were chosen and questionaire was used to conduct the investigation. Results 15 338 different type lavatories were constructed, of which the indoor toilets occupied 71.70%, the outdoor toilets occupied 28.30%. About 11.19 million yuan RMB was spent on the program, the average cost of each lavatory was 729.60 yuan RMB. Conclusion The program of lavatory improvement in the rural areas has been finished in Shandong Province in 2005, the data are satisfactory.

Objective To provide the morphology data for the study on the molecular mechanism of tumor metastasis,we observed the expression of podoplanin on the blood vessels and lymphatics in the human colonic carcinoma.Methods We observed the expression of podoplanin on the blood vessels and lymphatics in the colonic carcinoma from the patients of the operation in the defferent stages with the methods of HE staining and podoplanin immunohistochemistry staining. Results Podoplanin has not been stained,but podoplanin was positively expressed on the colonic carcinoma lymphatics.And, The podoplanin expression positive rate of the colonic carcinoma were increased along with the increase of tumor stages and occurence of lymph nodes metastasis. Conclusion Podoplanin choosed to express in lymphatics in the colonic carcinoma and could correctly distinguish the blood vessels and lymphatic vessels,could be regarded as the specific marker to mark lymphatic vessel. And, The podoplanin expression positive rate of the colonic carcinoma were increased along with the increase of tumor stages and occurence of lymph nodes metastasis.It indicates,It was correlated with colonic carcinoma lymphatical metastasis that the count of lymphatic vessels were increased in the colonic carcinoma along with the tumor advancing.

Anesthesia modalities for endovascular treatment of acute ischemic stroke include general anesthesia and conscious sedation.Several recent studies have shown that the general anesthesia is associated with the poor outcome of endovascular treatment in patients with acute ischemic stroke.The underlying reason of causing worse outcome is unknown.It may be associated with hemodynamic instability and hypotension.Currently,it is considered that both general anesthesia and conscious sedation have their advantages and disadvantages.We must choose individualize anesthesia modalities for each patient before we obtain highquality evidence.

Objective To study the expression of CD105 in laryngeal carcinoma and to evaluate the clinical significance of microvessel density(MVD).Methods Pathologic paraffin-embedded sections and clinical data from 40 patients with primary squmamous cell carcinoma of larynx were studied through immunohistochemistry.Microvessel density(MVD) highlighted by CD105 and CD34 were counted.Results CD105 expression in tumour tissue was significantly higher than normal health mucosa.The mean CD105-MVD value of T3 and T4 tumours showed a significantly deeper staining than T1 and T2;The mean CD105-MVD value of tumours with metastasis was also markedly higher than tumours with no metastasis;The diffenerce of mean CD105-MVD value between in 16 patients with recurred laryngeal carcinoma and in 20 patients with unrecurred laryngeal carcinoma for two years of follow-up was markedly significant(P

Objective: To evaluate the influence of fusing conditions on the surface structure and morphology of the SP1 SiO2. Methods:The SP1 SiO2 samples were sintered under 500, 650, 800, 950 and 1 100 ℃ at a rate of 250 ℃/h and maintained for 10, 30 min, 3 h respectively. The surface structure was characterized by FT-IR. Results:The characteristic peak of Si-OH bond at 958 cm-1 took off gradually with the rising temperature and prolonged time. Under the conditions of 950 ℃ maintained for 10 min and 30 min, and 650 ℃ maintained for 3 h, it disappeared completely. The particles of the SP1 SiO2 became larger while the size distribution became narrow. Conclusion: Fusing conditions can affect the Si-OH content and the size of the SP1 SiO2, as well as the size of distribution.[

Objective To understand the situation of rebuilding sanitary latrine in the rural areas in Qingdao and to evaluate the effect of sanitary latrine improvement, explore the new measure of sanitary latrine improvement in the rural areas. Methods In 2005, the typical method of investigation was used to choose 365 farmers from 8 rebuilding sanitary latrine villages among three counties in Qingdao, and to conduct on-site survey and health questionnaires on the construction of household latrines and excreta treatment, sanitation and related knowledge. Hygienic evaluation was made in accordance with hygienic standard for rural household latrine. Results 100% of the houses had the household latrines, 80.0% were the sanitary latrines, dejecta treatment rate was 78.36%. Conclusion All evaluation indexes reach the high level, the effect of household latrine improvement in the investigated rural areas is satisfactory.

OBJECTIVE To provide evidence to establish properly protocol for medical cosmetic institutions.METHODS Through investigation to analyze the current situation and problems of disinfection and isolation in the medical cosmetic institutions.RESULTS There were 86.7% medical cosmetic institutions located in commercial buildings.And there were 33.3% institutions with improper layout of therapeutic room,sterilizing room and dressing room resulting from insufficient space or less rooms.The illumination intensity of the ultraviolet lamp and the disinfection effect of air were not coincident with standards.CONCLUSIONS There are many problems of disinfection and isolation in the medical cosmetic institutions,the disinfection is insufficient,the ultraviolet light is in wild-using,people only focus on using,but ignore irradiation dose and distance.It is very important for strictly observing Hospital Infection Management Methods,Disinfection Technology Standards and Disinfection Management Methods,and strengthening the management of disinfection and isolation in the medical cosmetic institutions.

Objective:To explore the factors which lead to pharyngo-cutaneous fistula after total laryngectomy.Method:A retrospective study of 125 patients who underwent total laryngectomy during a 16-year period was carried out.Result:The rate of post-laryngectomy pharyngo-cutaneous fistula is 16.0% (20/125),it appears reduced tendency.With single-factor analysis,the factors effect on the rate of post-laryngectomy pharyngo-cutaneous fistula include clinical tumor stage,tumor types,pre-operative radiotherapy dose,intra-operative blood transfusion,operative duration.With all varible logistic model analysis,the factors effect on the rate of pharyngo-cutaneous fistula are clinical tumor stage and pre-operative radiotherapy dose,but pre-operative radiotherapy is very possible to be a risk factor (P=0.0566).With logistic model stepwise regression analysis,the factors effect on the rate of pharyngo-cutaneous fistula are clinical tumor stage and operative duration.Conclusion:Pharyngo-cutaneous fistula appears easily in patients with T3、T4 tumor,and have pre-operative radiotherapy, especially have large dose preradiotherapy.For these high risk patients,the duration of operation should be as short as possible to reduce the rate of post-laryngectomy pharyngo-cutaneous fistula.