Objective To investigate the clinical significance the clinical significance of the changes of ser-um tumor necrosis factor-α(TNF-α),nitric oxide(NO),interleukin-1β(IL-1β),and interleukin-6(IL-6)in pa-tients with colorectal cancer during perioperative period.Methods A total of 73 patients with colorectal cancer who underwent radical surgery in our hospital during December 2014 to December 2016 were selected as the observation group.At the same time,42 cases of healthy persons in our hospital from December 2014 to De-cember 2016 were selected as control group.The 5 mL of peripheral venous blood was collected 2 days after admission and 3 days after admission,and serum was separated.The levels of TNF-,IL-1 and IL-6 were meas-ured by enzyme-linked immunosorbent assay(ELISA).The level of NO was measured by nitric acid reduction method(mL).Results The serum levels of TNF-α,IL-1β,IL-6 content in the observation group were higher than those in the control group,and the content of NO was lower than that of control group,and the difference was statistically significant(P<0.05);there were no significant differences between the serum TNF-α,NO, IL-1β,IL-6 levels of colon and rectal cancer patients(P>0.05);TNF-α,IL-1β,IL-6 levels of patients at I and II phase were lower than those of patients at III and IV phase,while the content of NO was higher than that of patients at III and IV phase,and the difference was statistically significant(P<0.05);serum TNF-α,IL-1β, IL-6 levels after the operation were lower than those before operation,and the content of NO was higher than that before operation,and the difference was statistically significant(P<0.05).Conclusion The levels of ser-um TNF-a,IL-1 beta and IL-6 in the patients with colorectal cancer were increased with the severity of the disease,w hile the NO decreased with the severity of the disease.