Objective To investigate the effects and mechanisms of LINC00657 on oxidative glu-cose deprivation(OGD)-induced injury in mouse hippocampal neurons.Methods Mouse hippocam-pal neuron cell line HT22 was given OGD treatment to establish an injury model,with normally cul-tured HT22 cells as controls.The si-NC,si-LINC00657,microRNA(miR)-NC,and miR-224-3p mimics were transfected into HT22 cells,followed by OGD treatment.Co-transfection of si-LINC00657 and anti-miR-NC,or co-transfection of si-LINC00657 and anti-miR-224-3p,was performed in HT22 cells before OGD treatment.Real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR)was used to detect the relative expression levels of LINC00657 and miR-224-3p.CCK-8 assay and flow cytometry were used to detect cell viability and apoptosis rate,respectively.Kits were used to detect the activities of lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and the level of malondialdehyde(MDA).Dual-luciferase reporter gene assay was used to detect the effect of miR-224-3p overexpression on the luciferase activity of wild-type LINC00657 vector(WT-LINC00657)and mutant LINC00657 vector(MUT-LINC00657).Results Compared with controls,the expression of LINC00657 was upregulated and the expression of miR-224-3p was downregulated in OGD-induced HT22 cells(P＜0.05).Compared with trans-fection of si-NC or miR-NC,transfection of si-LINC00657 or miR-224-3p mimics resulted in in-creased cell viability,SOD activity,and GSH-Px activity,as well as decreased apoptosis rate,LDH activity,and MDA level(P＜0.05).Overexpression of miR-224-3p reduced the luciferase activity of WT-LINC00657(P＜0.05).Compared with cells co-transfected with si-LINC00657 and anti-miR-NC,cells co-transfected with si-LINC00657 and anti-miR-224-3p showed decreased cell viabili-ty,increased apoptosis rate,increased LDH activity and MDA level,and decreased SOD and GSH-Px activities(P＜0.05).Conclusion Interference with LINC00657 can promote cell proliferation,inhibit apoptosis and oxidative stress response by upregulating miR-224-3p,thereby alleviating OGD-induced injury in mouse hippocampal neurons.

Objective To investigate the effects and mechanisms of LINC00657 on oxidative glu-cose deprivation(OGD)-induced injury in mouse hippocampal neurons.Methods Mouse hippocam-pal neuron cell line HT22 was given OGD treatment to establish an injury model,with normally cul-tured HT22 cells as controls.The si-NC,si-LINC00657,microRNA(miR)-NC,and miR-224-3p mimics were transfected into HT22 cells,followed by OGD treatment.Co-transfection of si-LINC00657 and anti-miR-NC,or co-transfection of si-LINC00657 and anti-miR-224-3p,was performed in HT22 cells before OGD treatment.Real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR)was used to detect the relative expression levels of LINC00657 and miR-224-3p.CCK-8 assay and flow cytometry were used to detect cell viability and apoptosis rate,respectively.Kits were used to detect the activities of lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and the level of malondialdehyde(MDA).Dual-luciferase reporter gene assay was used to detect the effect of miR-224-3p overexpression on the luciferase activity of wild-type LINC00657 vector(WT-LINC00657)and mutant LINC00657 vector(MUT-LINC00657).Results Compared with controls,the expression of LINC00657 was upregulated and the expression of miR-224-3p was downregulated in OGD-induced HT22 cells(P＜0.05).Compared with trans-fection of si-NC or miR-NC,transfection of si-LINC00657 or miR-224-3p mimics resulted in in-creased cell viability,SOD activity,and GSH-Px activity,as well as decreased apoptosis rate,LDH activity,and MDA level(P＜0.05).Overexpression of miR-224-3p reduced the luciferase activity of WT-LINC00657(P＜0.05).Compared with cells co-transfected with si-LINC00657 and anti-miR-NC,cells co-transfected with si-LINC00657 and anti-miR-224-3p showed decreased cell viabili-ty,increased apoptosis rate,increased LDH activity and MDA level,and decreased SOD and GSH-Px activities(P＜0.05).Conclusion Interference with LINC00657 can promote cell proliferation,inhibit apoptosis and oxidative stress response by upregulating miR-224-3p,thereby alleviating OGD-induced injury in mouse hippocampal neurons.

Objective:To investigate the efficacy and safety of combined catheter-directed thrombolysis and anticoagulation for acute pulmonary embolism combined with lower limb deep vein thrombosis.Methods:A retrospective analysis was conducted using a case-control study approach on the clinical data of 58 patients with acute pulmonary embolism who received treatment at the Inner Mongolia Autonomous Region People's Hospital from August 2021 to August 2023. These patients were categorized into two groups based on whether they had lower-limb deep vein thrombosis or not. The group with pulmonary embolism alone ( n = 14) underwent targeted catheter thrombolysis in combination with anticoagulation treatment using low molecular-weight heparin. The group with both pulmonary embolism and deep vein thrombosis in the lower limbs (combined group, n = 44) received inferior vena cava filter placement, along with targeted catheter thrombolysis and anticoagulation treatment with low-molecular weight heparin. The improvement in pulmonary circulation was observed before and after the surgical interventions in both groups. A comparison was made regarding the improvement in indicators including arterial oxygen pressure, fingertip oxygen saturation, the proportion of pulmonary artery trunk thrombosis area to pulmonary artery trunk area, and respiratory rate, both before and after pulmonary artery catheterization and thrombolysis in both groups. Results:The surgery was successful for all patients. The symptom of shortness of breath was obviously relieved after surgery. After surgery, in the pulmonary embolism alone group, the respiratory rate was (22.75 ± 4.38) beats/min, the mean pulmonary artery pressure was (32.53 ± 3.63) mmHg (1 mmHg = 0.133 kPa), the arterial oxygen partial pressure was (81.46 ± 7.24) mmHg, and the fingertip oxygen saturation was (90.53 ± 4.57)%, respectively. In the combined group, the respiratory rate, mean pulmonary artery pressure, arterial oxygen partial pressure, and fingertip oxygen saturation were (23.35 ± 5.8) beats/min, (31.34 ± 4.53) mmHg, (82.34 ± 4.62) mmHg, and (92.57 ± 3.45)%, respectively. After surgery, the respiratory rate, pulmonary artery pressure, arterial oxygen partial pressure, and fingertip oxygen saturation in each group were significantly higher compared with the corresponding levels before surgery ( t = 6.33, 12.12, 17.32, 8.13, 6.86, 13.02, 15.52, 7.20, all P < 0.001). The proportion of pulmonary artery trunk thrombosis area to pulmonary artery trunk area in the group with pulmonary embolism alone was (19.56 ± 7.53)%, while in the combined group, it was (20.63 ± 4.83)%. After surgery, the proportion of pulmonary artery trunk thrombosis area to pulmonary artery trunk area in each group decreased compared with the corresponding value before surgery ( t = 19.36, 18.23, both P < 0.001). Conclusion:The surgical method combining catheter-directed thrombolysis with inferior vena cava filter placement is effective and safe for the treatment of acute pulmonary embolism combined with deep vein thrombosis in the lower limbs.