1.Argon plasma coagulation in the management of polyp remnants in colorectum
Shuguang ZHAO ; Xuxia WANG ; Baomin ZHAO
Chinese Journal of Tissue Engineering Research 2007;0(09):-
AIM: Remnant polyp tissues are often found after polypectomy. In this study, we assessed the outcome and safety of argon plasma coagulation (APC) in the management of colorectal sessile or semipedunculated polyp remnants after polypectomy. METHODS: Sixty-one colorectal polyps of 33 patients underwent polypectomy in Department of Digestion Medicine, Tangdu Hosptial, the Fourth Military Medical University of Chinese PLA from 2004 to 2007. Colorectal polyps type was identified by pathological examination. All polyps were removed under colonoscope with the diathermic snare, and the polyp remnants were destroyed with APC (Erbe, Germany). The power output was initially set at 40-50 W. In case of visibly insufficient coagulation, the output power was increased gradually by 5 W up until the remnants were completely ablated. Follow-up endoscopies were performed 1 and 3 months after the treatment. Pathological examination and APC treatment were performed when the recurrence of polyps was found. RESULTS: ①All 61 colorectal polyps were removed with the diathermic snare and the polyp remnants were destroyed with APC successfully only once. ②During the follow-up averaged 2.4 months, effective destruction of remnant polyp tissue was obtained in 59 polyps (96.7%) in 31 patients (93.9%). Colorectal polyp of two cases relapsed one month after treatment and identified as villous adenoma. ③Final power output was positively correlated with lesion size (r =0.97, P
2.Infulence of regulating liver-qi and tonifying kidney recipes on hypothalamus-pituitary-ovary axis in rats under psychologic stress
Baomin HUANG ; Xihao WANG ; Guanting ZHANG
Chinese Traditional Patent Medicine 1992;0(02):-
AIM: To investigate the effect of relieving liver-qi recipes(Radix Bupleuri,Radix Paeoniae Alba,Fructus Aurantii,etc.) on the neuro reproductive endocrine in female rats under psychologic stress. METHODS: The psychologic stress induced syndrome of liver-qi stagnation in female rats was established by tail pinch.The hypothalamus pituitary and serum levels of NE,DA,5 HT,5-HIAA,DOPAC,HVA and the serum FSH,LH,E2,T,P,PRL in rats of any groups were measured. RESULTS: The levels of NE in hypothalamus pituitary and FSH,LH,E2,P in serum of model rats were lower and the contents of DA,5-HT,5-HIAA in hypothalamus pituitary and in serum and NE,T in serum were significantly higher than those of the normal control.The serum P and LH in the complex group were elevated.The descending NE in hypothalamus pituitary and FSH,E2 in serum increased and the ascended DA,5-HT,5-HIAA in hypothalamus pituitary and NE,5-HT,T in serum significantly descresed in the shugan group and the complex group.The enhance of NE in hypothalamus pituitary and FSH,P in serum and the reduction of 5-HT in hypothalamus pituitary and 5-HT,NE,T in serum were also obversed in the bushen group. CONCLUSION: Regulating liver-qi and tonifying the kidney recipes could improve the function of neuro reproductive endocrine system which could be attenuated or disturbed by psychologic stress.
3.Diagnosis and treatment of testicular torsion (report of 17 cases)
Baomin QIAO ; Guang SUN ; Wencheng WANG
Chinese Journal of Urology 2001;0(09):-
Objective To study the diagnosis and treatment of acute testicular torsion. Methods 17 cases of testicular torsion treated from January 1995 to December 2000 were reviewed.The diagnosis and treatment were analysed. Results The diagnosis of testicular torsion in all the 17 cases was assessed on color Doppler ultrasonics.1 case was cured by manual detorsion,2 by surgical detorsion and orchidopexy,14 by orchiectomy and contralateral orchidopexy.The fertile ability of 4 cases over 18 years old was observed,3 being normal and 1 slightly below normal. Conclusions Color Doppler ultrasonics examination can serve as a chief adjuvant diagnostic method.All cases with a torsion more than 360? and longer than 24 h will have the ipsilateral testicular loss,whereas in case the torsion is less than 360? and less than 24 h have a chance to preserve the testis by surgical detorsion and orchidopexy.
4.CT Diagnosis of Granulomatous Diseases in Chest
Fazhan ZHOU ; Baomin WANG ; Duoling CHEN
Journal of Practical Radiology 2001;0(01):-
Objective To evaluate CT diagnosis and differential diagnosis of thoracic granulomatous diseases.Methods 13 cases of thoracic granulomatous diseases(tuberculoma 5,mycosis 3,sarcoidosis 2,Wegener's granuloma 2,eosinophilic granuloma 1) proved surgically-pathologically together with clinical and imaging data undergone analysis,especially put emphasis on CT features.Results ①Pulmonary granulomatous lesions commonly presented as nodules or masses,single or multiple,all the lesions had cavity,as well known pulmonary tuberculoma,pulmonary mycosis and Wegener's granuloma.②Cavity associated with mobile mass inside presented as air meniscus or air sing sign were the imaging characteristics of pulmonary aspergilloma.③Hilar and mediastinal lymphadenectasis always presented as bilaterally and symmetrically that was the typical sign of stage I sarcoidosis.④The multiple,multiplicity together with necrosis and cavity were the characteristics of wegener's granuloma.⑤Nodules and pneumtoceles were the major specific signs of pulmonary eosinophilic granuloma.Conclusion To analyse CT specific features and in combination with clinical data,it can improve the diagnostic accuracy of pulmonary granulomatous diseases.
5.Synthesis of seven sinapine analogs and their effects on the tension of smooth muscle of intestines isolated from rabbit.
Yue AN ; Ying ZHANG ; Huiguo WANG ; Baomin FENG
Acta Pharmaceutica Sinica 2011;46(12):1466-70
Seven sinapine analogs (6a-6g) were synthesized using cinnamon acid or benzoic acid and their derivatives as starting materials, which obtained from substituted benzaldehyde and malonate. The structures of target compounds were characterized by IR, 1H NMR and elemental analysis. The effects of compounds 6a-6g on the smooth muscle of intestine isolated from rabbit were studied, and the experimental results showed that compounds 6a, 6d and 6g had diastolic action, while 6f had contractile action.
6.Antibody preparation and enzyme linked immunosorbent assay of Lonicera japonica phenylalnine ammonia lyase.
Zhouyong WANG ; Luqi HUANG ; Yuan YUAN ; Baomin WANG
Acta Pharmaceutica Sinica 2013;48(9):1498-502
The expression of phenylalnine ammonia lyase (LJPAL1) is closely related to the content of active compounds in Lonicera japonica. In this paper, a prokaryotic expression vector is constructed and LJPAL1 protein is expressed in E. coli. Three antigen sites were synthesized to peptide antigen and prepared polyclonal antibody of Anti-LJT-1, Anti-LJT-2 and Anti-LJT-3, separately. Antibody Anti-LJT-2 was screened using Western blotting. And indirect ELISA was built using Anti-LJT-2. The results of this study will be a base for honeysuckle chemical quality and evaluation kits.
7.Expression of CyclinD1 protein in nephroblastoma
Hongfang WU ; Baomin FU ; Xiaoming CHEN ; Zhongmin WANG
Journal of Clinical Pediatrics 2010;(1):73-75
Objective To investigate the expression of CyelinD1 protein in nephroblastoma.Methods The tissue microarray were made in 28 cases of nephroblastoma and 19 cases of renal tissue adjacent to tumor.The expression of CyclinD1 and p53 proteins were detected by immunohistochemistry.Results The positive rate of the expression of CyclinD1 and p53 protein was significantly different between nephroblastoma and renal tissue adjacent to tumor (P < 0.05).The positive rate of the expression of CyclinD1 and p53 protein was significantly different in different degree of differentiation of nephroblastoma (P < 0.05).The expression of CyclinD1 and p53 protein was positively correlated (P < 0.05).Conclusions The CyclinD1 and p53 might be used as the marks for estimating the degree of differentiation of nephroblastoma.The over-expression of CyclinD1 and the mutation of p53 might play the vital role in nephroblastoma.
8.Chemical constituents from roots of Stellera chamaejasme
Baomin FENG ; Yuehu PEI ; Hailong ZHANG ; Huiming HUA ; Yongq WANG
Chinese Traditional and Herbal Drugs 1994;0(01):-
Object To study the chemical constituents from the roots of Stellera chamaejasme L. Methods Chemical constituents were isolated by chromatographic methods and identified by physiochemical and spectral analysis. Results One lignan and three biflavonoids were purified and determined as bursehernin (Ⅰ), chamaejasmenin B (Ⅱ), isoneochamaejasmin A (Ⅲ),and (+)-chamaejasmin (Ⅳ). Conclusion Compound Ⅲ and Ⅳ are determined as new optical compounds. Compound I is first isolated from Stellera L. species.
9.Purification Technology of Camellia Saponin Using D101 Macroporous Adsorption Resin
Hongbing LI ; Ling TANG ; Baomin FENG ; Yongqi WANG
China Pharmacy 2001;0(12):-
OBJECTIVE:To study thc purification technology of camellia saponin using D101 macroporous adsorption resin.METHODS:The loading volume of sample and the adsorption quantity of D101 macroporous adsorption resin and the concentration and volume of alcohol in eluting water -soluble foreign substances and total cemallia saponins were investigated taking the content of total cemallia saponins as index.RESULTS:The loading volume of sample of D101 macroporous adsorption resin was 3 fold that of resin D101(or 3 BV,resin bed volume) and its adsorption quantity was 566 mg?g~(-1)(crude drug);3 BV distilled water and 1 BV 20%ethanol were used for the elution of the water-soluble foreign substances,and 3BV of 85%ethanol was used for the elution of total cemallia saponins.CONCLUSION:D101 macroporous resin has good purification efficacy for camellia saponins.The purification process is simple with low cost,and it is applicable for large - scale industrialized production.
10.Soluble Proteins SDS-PAGE of C.tubulosa(Schenk) R.Wight
Pei CHEN ; Yuhai GUO ; Baomin WANG ; Zhixi ZHAI ;
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(07):-
Objective: To identify 5 specimens of C.tubulosa(Schenk) R.Wight on different parasitifers,and explore the relationship between electrophoretic fingerprint and identification on soluble-protein,meanwhile providing data for establishing electrophoresis pattern library of Chinese crude drugs.Methods: The electrophoretic characteristics of soluble-proteins from 5 specimens of C.tubulosa(Schenk) R.Wight on different parasitifers were studied with SDS-PAGE.Results: It indicated that reproducible and high resolvable electrophoresis patterns of soluble proteins of C.tubulosa(Schenk) R.Wight on different parasitifers by SDS-PAGE.Their degrees of polymorphism were 55.56%.Conclusion: Each specimen had its own unique band pattern distinguish from the others.The electrophoresis pattern of SDS-PAGE of soluble proteins can be regarded as protein fingerprints for identification of C.tubulosa(Schenk) R.Wight on different parasitifers.