Objective To report surgical outcomes of managing comminuted fractures of the inferior patellar pole with separate vertical wiring plus anchor suture fixation.Methods From January 2010 to January 2015,37 patients with comminuted fractures of the inferior patellar pole were treated with separate vertical wiring and anchor suture fixation.They were 15 men and 22 women,from 32 to 76 years of age (average,55.1 years).The intervals from fracture to surgery ranged from 2 to 3 days (average,2.1 days).According to the AO classification,all the fractures were type 34-A1.The visual analogue scale (VAS) scores,ranges of motion (ROM) of the knee,Bostman scores and postoperative complications were recorded at 6 weeks,3,6 and 12 months and the final follow-up postoperatively.Results The follow-up time for the 37 patients averaged 16 months (range,from 12 to 23 months).The fracture union time averaged 10 weeks after surgery (range,from 8 to 13 weeks).At postoperative 6 weeks,3,6 and 12 months and the final follow-up,respectively,the average VAS scores were 2.5,1.1,0.3,0.2 and 0.2 points,the average ranges of motion 109.5°,123.7°,128.6°,129.1° and 132.5°,and the average Bostman scores 23.7,26.9,29.1,29.4 and 29.6 points.No patient reported delayed union,nonunion,loss of reduction,wire breakage,wound problem or irritation from the implant.Conclusion Separate vertical wiring combined with anchor suture fixation is a useful technique for comminuted fractures of the inferior patellar pole,for it is easy to perform,allows early functional exercise and leads to fine curative outcomes.

Objective To investigate the predictive value of National Institutes of Health Stroke Scale(NIHSS)score,serum brain-derived neurotrophic factor(BDNF)and interleukin-6(IL-6)in post-stroke depression(PSD).Methods A total of 180 patients with stroke were selected and divided into the PSD group(n=80,HAMD≥8 points)and the non-PSD(NPSD)group(n=100,HAMD＜8 points),according to HAMD score at 3 months after stroke.The basic information,NIHSS score,serum BDNF and IL-6 were compared between the two groups.The influencing factors of PSD were analyzed by Logistic regression method.Receiver operating characteristic(ROC)curves were plotted to evaluate the predictive value of NIHSS score,serum BDNF and IL-6 for PSD.Results Compared with the NPSD grope,low density lipoprotein cholesterol(LDL-C)and serum BDNF level were significantly lower,NIHSS score and serum IL-6 level were significantly increased in the PSD group(P＜0.05).Results of Logistic regression analysis showed that increased NIHSS score and serum IL-6 were risk factors for PSD,and increased serum BDNF was the protective factor in patents with PSD(P＜0.05).The area under curve(AUC)of NIHSS score,serum BDNF and IL-6 and their combination prediction of PSD were 0.762,0.746,0.796 and 0.839,respectively.The sensitivity of the combined prediction was 86.0%and a specificity was 95.0%.Conclusion Compared with NPSD patients,the NIHSS score and serum IL-6 level are increased,and the serum BDNF level is decreased in patients with PSD.The combination of all three has a high predictive value for patients of PSD.

Danshen, the dried roots and rhizomes of Salvia miltiorrhiza Bunge (S. miltiorrhiza), is widely used in the treatment of cardiovascular and cerebrovascular diseases. Tanshinones, the bioactive compounds from Danshen, exhibit a wide spectrum of pharmacological properties, suggesting their potential for future therapeutic applications. Tanshinone biosynthesis is a complex process involving at least six P450 enzymes that have been identified and characterized, most of which belong to the CYP76 and CYP71 families. In this study, CYP81C16, a member of the CYP71 clan, was identified in S. miltiorrhiza. An in vitro assay revealed that it could catalyze the hydroxylation of four para-quinone-type tanshinones, namely neocryptotanshinone, deoxyneocryptotanshinone, and danshenxinkuns A and B. SmCYP81C16 emerged as a potential broad-spectrum oxidase targeting the C-18 position of para-quinone-type tanshinones with an impressive relative conversion rate exceeding 90%. Kinetic evaluations andin vivo assays underscored its highest affinity towards neocryptotanshinone among the tested substrates. The overexpression of SmCYP81C16 promoted the accumulation of (iso)tanshinone in hairy root lines. The characterization of SmCYP81C16 in this study accentuates its potential as a pivotal tool in the biotechnological production of tanshinones, either through microbial or plant metabolic engineering.
Humans ; Salvia miltiorrhiza/metabolism* ; Biosynthetic Pathways ; Quinones/metabolism* ; Plant Roots/metabolism* ; Gene Expression Regulation, Plant