BACKGROUND: Biomedical materials contact internal environment of human body, and sometimes are implanted into organism. Therefore, they should have biocompatibility, chemical stability, suitable physical mechanical function and simple processing and molding, but no toxicity.OBJECTIVE: To investigate the preparation of biomedical polymer anticoagulant materials in the aspects of bioinert material, biological active surface, albumin structure and application in anticoagulation.METHODS: A computer-based online search of PubMed and Wanfang database was performed for articles related to preparation of biomedical polymer anticoagulant materials published between 1969 and 2010.RESULTS AND CONCLUSION: Currently preparation of anticoagulant materials commonly utilizes bioinert surface or bioactive surface alone, which has obtained good effects, but the biocompatibility, such as blood compatibility, cannot be retained for a long period of time. The combination of bioinert surface and bioactive surface plus albumin, natural constitutions in human blood may be the trend of anticoagulant materials development. Polyethylene glycol with high bioinert property in combination with albumin recognition factor cibacron blue with high bioactivity can be used to prepare active modifier, which is used to modify polyurethane.

BACKGROUND:Cardiovascular biomaterials applied under the blood-contact conditions must have anti-thrombotic, anti-biodegradable and anti-infective properties. OBJECTIVE:To develop novel polymer materials for implantation and intervention in cardiovascular tissue engineering and then to explore the biological, blood and cellcompatibilities of corresponding surface-modified polymer biomaterials based on surface construction and biological response.
METHODS:We retrieved PubMed and WanFang databases for relevant articles publishing from 1984 to 2013. The key words were“biocompatibility, blood compatibility, biomedical materials, biomedical polymer materials”in English and Chinese, respectively.
RESULTS AND CONCLUSION:Here, we analyze the fol owing four aspects:protein adsorption, biometric identification in celladhesion, and the“waterfal model”for enzyme catalysis during blood coagulation and fibrinolysis. Consequently, it is concluded that the functional surface construction of polymer biomaterials and research on corresponding biocompatibility and endothelial cellcompatibility are crucial for developing novel polymer materials for implantation and intervention in cardiovascular tissue engineering. Through in-depth studies of the types and applications of polymer biomaterials, cardiovascular medical devices and implantable soft tissue substitutes, the differences between the surface and the body wil be reflected in the many layers of molecules extending from the surface to the body. Two major factors, surface energy and molecular mobility, determine the body/surface behaviors that include body/surface differences and phase separation. Considering the difference between body/surface composition, an additional determinant is indispensable, that is the crystal ization behavior of each component.

BACKGROUND:As the cardiovascular device, biomaterials applied under the blood-contact conditions should have anti-thrombotic, anti-biodegradable and anti-infective properties. OBJECTIVE:To review the research progression in polymer materials for implantation and intervention in cardiovascular tissue engineering and to explore the biocompatibility, blood compatibility and cytocompatibility of the surface-modified polymer biomaterials based on the surface endothelialization using tissue engineering techniques. METHODS:We retrieved PubMed and Wanfang databases for relevant articles publishing from 1963 to 2015. The key words were“Biocompatibility, Blood compatibility, Biomedical Materials, Biomedical polymer materials”in English and Chinese, respectively. Those unrelated, outdated and repetitive papers were excluded. Literatures addressing the blood compatibility, biocompatibility, and cytocompatibility of the surface-modified polymer biomaterials based on the surface endothelialization using tissue engineering techniques were investigated by summarizing function of vascular endothelial cel s, tissue-engineered endothelial cel s on the implant surface, fixation of cel growth-promoting factor on the surface of polymeric biomaterials, and endothelialization of the material surface. RESULTS AND CONCLUSION:Total y 71 relevant articles were included. The tissue-engineered modification of endothelial cel s on the surface of polymer biomaterials and their biocompatibility and cel compatibility are crucial for developing novel polymer materials for implantation and intervention in cardiovascular tissue engineering. Through in-depth studies of the types and applications of polymer biomaterials, cardiovascular medical devices and implantable soft tissue substitutes, the differences between the surface and the body wil be reflected in the many layers of molecules extending from the surface to the body. Two major factors, surface energy and molecular mobility, determine the body/surface behaviors that include body/surface differences and phase separation. Considering the difference between the body/surface composition, an additional determinant is indispensable, that is, the crystal ization behavior of each component.

Icariin and total flavonoids in "Xianlinggubao Capsule", a Chinese herbal preparationcontaining Epimedium L. as the main ingredient for the treatment of osteoporosis, were determined quan-titatively by HPLC and UV spectrophotometry respectively. It was found that the contents of icariin were4.16～27.67 mg/g and that of flavonoids were 62. 56～109.78 mg/g. The recoveries were 96.17% and103.58% respectively. The method could be used for the quality control of the drug.

Surface physical chemical properties of tissue-engineered materials are greatly important for histocompatibility of the materials. Therefore, surface modification based on original physical mechanical performance could promote cell attachment and growth or bioactive molecule, and significantly improve material cell compatibility. To date, plasma and grafting has become main methods of surface modification of polymers. This paper introduced plasma and grafting methods of surface modification of materials and the application in tissue engineering.

To purify the protein encoding the small capsid protein (SCP) of KSHV and analyze its immunogenicity, the carboxyl terminus of orf65 of Kaposi's sarcoma associated-herpesvirus (KSHV) was expressed in a prokaryotic expression system. The expression of recombinant E. coli containing pQE-80L-orf65 was induced by isopropyl-β-D-thiogalactopyranoside (IPTG) and the fusion protein was purified by chromatography. The expressed protein and its purified product were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and showed that 9 kDa was the expected size of the purified orf65 protein. The antiserum was produced in rabbit which was immunized by purified orf65 protein. An ELISA assay was established to analyze the immunogenicity of the purified orf65 protein. The ELISA analysis demonstrated that orf65 protein has strong immune activity, and the immune activity of polyclonal antibody against orf65 was more than 4 fold higher than that in the serum of the non-immunized rabbit. These results demonstrate that purified orf65 protein has very strong immunogenicity and can be used in screening KSHV infection in the general population using ELISA.

BACKGROUND: The development of tissue engineering has provided a possibility for repairing and reconstructing tissues or organs. However, studies on biomedical tissue-engineered and polymer tissue-engineered materials need to be investigated. OBJECTIVE: To clarify the content of tissue engineering and the application of polymer material in tissue engineering from the point of biocompatibility. RETRIEVAL STRATEGY: Using the terms "tissue engineering, tissue engineering materials, Polymers materials, bio-compatibility, bio-compatibility materials, cell-compatibility, cell-compatibility materials", we retrieved PubMed database to identify studies published between January 1990 and December 2007 in the English language. At the same time, we searched Wanfang database with the same terms in the Chinese language. After primarily selected, 81literatures were kept. Inclusive criteria: studies, whose contents are related to biocompatibility of tissue-engineered materials. Exclusive criteria: repetitive studies or Meta analysis. Thirty literatures corresponded to the inclusive criteria, and fifty-one were rejected due to obsolete or repetitive contents. Among the 30 included literatures, 19 were about biocompatibility, and the remaining 11 about cellular compatibility materials. LITERATURE EVALUATION: The included studies were mainly from Pubmed database and Wanfang database. A total of 25 treatises and 5 reviews were kept. DATA SYNTHESIS: The content of tissue engineering consisted of seeded cell inoculation, biomaterial implanting and cell transplantation. Allogenic, autogenous, and xenogenous tissues were in vitro broken into cells, and then reconstructed through inoculation and proliferation by gene reconstruction technique. Much attention should be focused on how to reconstruct tissue-engineered materials with materials and living cells, I.e. To reconstruct active materials with biological functions. Tissue-engineered materials should have the best interface reaction effect between material surface and cells. Therefore, the core of studying tissue-engineered materials is to design a device, which has chemical molecular level and three-dimensional molecular level cell/material mixed surface, and also has a three-dimensional molecular level appearance corresponding to biomechanical requirement. Polymer materials have good physical mechanical functions, and their molecular structures are closer to living body. Therefore, polymer materials are widely used as biomaterials and exert an important role in the field of tissue engineering. CONCLUSION:To study biomaterials with good tissue compatibility is the basis for tissue engineering development. Polymer materials are widely used in the tissue engineering due to their good property and molecular structure closer to living body.

BACKGROUND:To clarify the effects of topology of tissue engineering material surface on cell compatibility from random roughness.porosity,groove/ridge, fiber,texture and protein tracks 6 aspects. STUDY SOURCES: Using the terms"biocompatibility,biocompatibility materials,tissue engineering,tissue engineering materials.cell-compatibility and cell-compatibility materials",we searched the PUBMED database to identify studies published in the English language from January 1987 to January 2007. STUDY SELECTION:The data were selected primarily.The quotations in each paper ware looked for.Inclusive criteria: The contents stated in papers were related with biocompatibility of tissue engineering material. Exclusive criteria: repetitive study or Meta analysis papers.DATA EXTRACTION:Totally 74 related papers were collected.Thirty-two met inclusive criteria,among which,24 related with biocompatibility and 8 related with cell-compatibility materials.The other 42 papers were excluded due to obsolete or repetitive contents. DATA SYNTHESIS:①The interaction of tissue engineering material and living body:Various Interactions producing when high polymer tissue engineering material contacts with tissue of living body are reviewed. It js pointed out that the interaction of material and living body depends on the biocompatibility degree of material;The influences of materials on histocompatibility include microscopic molecular level and macroscopic scale level,moreover,the effect of macroscopic scale level (include the topology of material surface) is more important than chemical effect of microscopic molecular level.②Effects of physical and chemical properties on cell-compatibility of materials:The effects of topology of surface of random roughness,porosity,groove/ridge,fiber, texture and protein tracks 6 kinds of materials on cell-compatibility are reviewed. It is also pointed out that the influences are very important in studying the biocompatibility of tissue engineering material and designing tissue compatibility materials.CONCLUSION:Topology of material surface has great influences on cell-compatibility of material.The interaction of cells and polymer is an index to evaluate cell-compatibility of material.Short-term interaction degree of cell and polymeric material can be assessed by detecting the adhesion degree of cells and polymeric material surface,while long-term interaction by detecting the growth of cells cultured in vitro or polymeric material implanted in vivo.

OBJECTIVE: To discuss the influence of physical and chemical properties of tissue engineered material surface on the cell compatibility, involving the surface energy, hydrophilicity/hydrophobicity, chemical structure and active factors loaded on the material surface, point out that the physical and chemical properties of material surface have great influence on the cell compatibility of the material, i.e., explain the cell compatibility of tissue engineered materials.DATA SOURCES: An online search of Pubmed database was undertaken to identify relevant articles published in English from December 1997 to December 2006 using the keywords of "bio-compatibility, bio-compatibility materials, tissue engineering, tissue engineering materials, cell-compatibility". Meanwhile, Chinese relevant articles published from December 1997 to December 2006 were searched in Wanfang database with the same keywords in Chinese.STUDY SELECTION: The data were primarily checked. Inclusive criteria: articles about tissue-engineered materials of biocompatibility. The repetitive studies or Meta analysis were excluded.DATA EXTRACTION: Totally 71 relevant literatures were collected, 33 of which were accorded with the inclusive criteria, and the 38 repetitive ones or with old contents were excluded. Of the 33 involved literatures, 22 dealt with biocompatibility, and 11 with the cell-material compatibility.DATA SYNTHESIS: ① Interaction of tissue-engineered materials with organism: The various interactions of tissue-engineered polymer materials with organism are summarized. It is pointed out that the interactions of materials with organism decide the degree of material-tissue compatibility. The effects of material on the tissue compatibility result from the micromolecular and macroscopic levels, and the chemical effect of the macroscopic level is more important than that of the micromolecular one. ② Influence of the physicochemical properties of the material surface on the cell-material com coatibility:The influences on the cell-material compatibility by the chemical nature and structure, composition, energy, hydrophilicity/hydrophobicity, charges and active factorsloaded on the surface are summarized. The obtained information is the important contents for understanding the biocompatibility of the tissue engineered materials and designing biocompatible materials. CONCLUSION: The physical and chemical properties of the material surface greatly affect the cell-material compatibility. The interaction of cells with the polymer matrix is an index to evaluate the cell compatibility. The degree of the short-term interaction of cells with polymer materials can be evaluated by the degree of adhesion on the surface of the polymer materials, while the long-term interaction can be evaluated by detecting the growth of cells cultured in vitro or implanting the polymer material.

OBJECTIVE: Based on the mechanism of cellular adhesive growth and the developing course of cell compatibility materials, the cytological effects of tissue engineering materials of various polymers and surface topographies are reviewed. And presume theoretically that the materials of porous structure are better than smooth materials in surface, and biodegradation materials can explain the biocompatibility.DATA SOURCES: An online search was conducted in PUBMEDdatabase to identify the related articles published from December 1997 to December 2003 with the key words of "tissue engineering, tissue engineering materials, cell-compatibility, cell compatibility materials, procession anchorage", and the language was limited to English. Meanwhile, the related Chinese articles were retrieved in Wanfang database published at the same period by inputting the key words of "tissue engineering, tissue engineering materials, cell-compatibility, cell compatibility materials,mechanism of adhesive growth" into computer.STUDY SELECTION: All the data were checked primarily, and the quotations of each article were looked up. Inclusive criterion: content related to the cell-compatibility of tissue engineering materials. Exclusion criterion:repeated study or Meta-analysis.DATA EXTRACTION: Totally 41 articles were collected, and 28 ones were deleted due to the repeated or dated contents. Among the 13 articles met the inclusive criterion, 2 ones were about the mechanism of cellular adhesive growth and 11 ones were referred to the cell compatibility materials.DATA SYNTHESIS: ①The mechanism of cellular adhesive growth: To summarize the characteristics of adhesive growth in various in vitro cultured cells, and briefly describe the growth procedure of those cells cultured on substrate materials. Suggesting that compensate for the adverse effects caused by pore space structure, more effective methods should be adopted to form the transition layer of cell compatibility when the cells are required to grow.②Cell compatibility materials: To summarize the cytological effect produced by the materials of various polymers and surface topographies, and point out that the interaction mechanism between cells and materials of different surface topographies is still a difficult but valuable topic in the related fields. The physical and chemical properties as well as topological structure of the materials surface influence on the cell compati bility of materials. The interaction between cells andpolymers is the evalu ation indicator of cell compatibility of materials. The temporary interaction can be evaluated by the adhesion of cells to polymers surface, while the long-term interaction may be estimated by the growth of in vitro cultured cells or in vivo implanted polymers.CONCLUSION: Theoretically, porous materials show great superiority compared with smooth surface materials; Biodegradation materials can tackle the biocompatibility completely.