Objective: To select the optimum prepartion procedure for Sanhuangweixueping, Capsules. Methods: The optimum conditions were selected with the contents of active components: emodin, berberine, and baicalin as markers. Results: The optimum preparation procedure was: berberine was extracted with 95% alcohol. Rhizoma Bletillae was immersed with water, then precipitated with 70% alcohol. Radix et Rhizoma Rhei, Radix Scutellariae and Radix Glycyrrhizae were decocted, then precipitaed with 60% alcohol. Sanhuangweixueping Capsules prepared with the optimum preparation procedure possessed stronger pharmacological effect. Conclusion: The optimum prepartion procedure is reliable and the extraction ratio of active compoents is high.

Objective To discuss the relationship between FokI gene polymorphism and colorectal canc-er.Method The references that met the inclusive criteria were assessed .The extracted data were summarized in the form of tables .Data were analyzed by heterogeneity test .Meta-analysis was conducted by using fixed effects model or random effects model .Results Fifteen experimental studies were included in this study ,including 7, 859 colorectal cancer patients and 9,933 controls.The association between VDR gene FokI and colorectal cancer was expressed as the odds ratio(OR)and 95% confidence interval(CI).FokI recessive genetic model,ff versus FF+Ff,ORrecessive=1.01(95% CI=0.87~1.17,I2 =54%,P=0.91),ORdominant =0.94(95% CI=0.83~1.06,I2 =64%,P=0.30);allele model,fversus F,OR=0.98(95% CI=0.89~1.08,I2 =71%,P=0.72).In subgroup analysis,recessive genetic model,ORCaucasian race =0.95(95% CI=0.86~1.05,I2 =0%,P=0.33);ORyellow race=1.12(95%CI=0.77~1.63,I2 =72%,P=0.54).In subgroup analysis,dom-inant gene model,ORCaucasian race =0.95(95% CI=0.86~1.04,I2 =27%,P=0.29);ORyellow race =0.89(95%CI=0.65~1.22,I2 =78%,P=0.47).In subgroup analysis,allele model,ORCaucasian race=0.97 (95%CI=0.90~1.04,I2 =31%,P=0.38);ORyellow race =1(95% CI =0.79 ~1.27,I2 =82%,P=1.00).Conclusion Our results suggested that in VDR gene FokI ,F and f alleles were not obviously directly correlated with colorectal cancer .No difference was found between Asian and Caucasian .The conclusions in other populations should be further identified by extensive experimental studies .

Objective: To study the effect of zinc supplement on bone-contacting implant surface ratio (BCSR).Methods: 40 rabbits with titanium implants placed into the proximal tibial heads were divided randomly into zinc supplement group(n=20) and control group(n=20). 1% zinc sulfate in 0.9% saline was administered intramuscularly at the dose of 4 mg/(kg?d) for each tested animal, and the same amount of 0.9% saline was used for the controls. The animals were sacrificed 1, 2, 4, 8 and 12 weeks after treatment respectively.BCSR,bone zinc content and serum Cu/Zn values were measured with morphometry, atomic absorption photometry and atomic nebulizntion absorption method respectively.Results: Zinc supplement could significantly increase the BCSR value (P0.05). Conclusion: Moderate zinc supplement may increase the bone-contacting implant surface ratio, but not alter the zinc metabolism.

There was almost one hundred years since surgical stapler invention to now. Using stapler (stitching instrument) in surgery has many advantages, such as stitching fast, easy to operating, few side effects and complications. It makes tumor excision surgery can’t be cut focus in past possible and improving the curative effect of the surgery. Surgical staplers also give difficult surgery and new technology strong supports. In the last ten years, for cheap price and rapid development of the technology level, domestic staplers’ market share is increasing year by year and breaking the foreign monopoly product. In open surgery, domestic stapler has progressively more than foreign brands. In endoscopy surgery, domestic staplers also have a place. In current, surgical staplers are used more and more popular in the domestic tertiary medical institutions and becoming indispensable tools in many surgeries. Surgical staplers (stitching instrument) are broadly used in esophageal reconstruction, gastrointestinal tract reconstruction, endoscopic surgery in pulmonary bulla resection, uterus resection, pancreas resection, duodenum resection

Objective To investigate the effects of malnutrition, nephrosis itself and glucocorticoid therapy on serum growth hormone binding protein(GHBP) and liver growth hormone receptor(GHR), and elucidate the relationship between growth failure in nephrotic rats and serum GHBP or liver GHR. Methods Twenty-four male Sprague-Dawley(SD) rats were randomly divided into control, pair-fed, doxoruhincin-induced nephrotic (nephrotic) and dexamethasone-treated nephrotic (des-treated ) rats. Serum GHB P, GHB P- 1 and liver GHR were measured by dextran-coated charcoal technique, gel filtration and radioreceptor assay respectively. Results (1) Serum GHBP and liver GHR were reduced in nephrotic and des-treated rats compared with control and pair-fed rats, but no significant difference was found between control and pair-fed rats or between nephrotic and des-treated rats. (2)Serum GHBP-1 was lower in pair-fed rats, even lower in nephrotic rats and lowest in des-treated rats than in control. (3) There was some significantly positive correlations between nose-tail length or weight and serum GHBP or liver GHR. Conclusion GH resistance, due to decreasd liver GHR, is an important cause of growth failure induced by secondary malnutrition and nephrosis itself Glucocorticoid therapy deteriorates growth failure by further decreased hepatic GHR.

Objective:To study the effect of bone marrow stroma cell( BM SC) transplantation combined with multi-pore mineralization ossein(Bio-Oss) in the plerosis of mandibular defect.Methods:A round whole-layer defect in the diameter of 1.5 cm was created on each side of mandible in 16 whit e New Zealand rabbits.The rabbits were randomly divided into two groups.In group 1 BMSCs were collected and cultured with mineralization induce medium for 2 wee ks. Then cell suspension at the density of 5?10 7 /ml and gelatum were mixed a nd dripped into Bio-Oss bones, incubated in DMEM in 50 ml/L CO 2 incuba tor at 37 ℃ for 4-6 h.The BMSC-Bio-Oss was applied to make plerosis of the m andibular defects.In group 2 the defects were repaired with the Bio-Oss ossein only.Samples were collected after 8 and 12 weeks after opreation respectively an d observed with radiology and histology.Results:12 weeks after o peration, (1)in group 1 previous defects were basically consisted of new bone tissue;(2)in group 2 the previous defects were mainly consisted of fibrous tis sue. Conculsions:BMSC-Bio-Oss transplantation may effectively repair bone defect.

Objective:To study the safety and practicability of probiotics intestinal preparation combined with early enteral nutrition in perioperative period of laparoscopic rectal cancer radical proctectomy.Methods:The clinical data of 131 patients who underwent selective laparoscopic rectal cancer radical proctectomy in Shengjing Hospital of China Medical University from January to June 2018 were retrospectively analyzed. Among them, 80 patients received probiotics intestinal preparation combined with early enteral nutrition (experimental group), and 51 patients received traditional intestinal preparation and postoperative treatment (control group). The bleeding volume during operation, hospitalization expenses, first exhaust time, postoperative hospitalization time, indwelling catheter time and complications were compared between 2 groups.Results:The bleeding volume during operation, hospitalization expenses, postoperative hospitalization time in experimental group were significantly less than those in control group: (166.33 ± 15.18) ml vs. (226.47 ± 16.46) ml, (42 365 ± 3 921) yuan vs. (51 048 ± 4 772) yuan and (13.32 ± 1.03) d vs. (13.98 ± 1.29) d, and there were statistical differences ( P<0.01). There were no statistical difference in the first exhaust time, indwelling catheter time and incidence of complications between 2 groups ( P>0.05). Conclusions:The probiotics intestinal preparation combined with early enteral nutrition in perioperative period can reduce the hospitalization expenses and shorten the hospitalization time in patients undergoing laparoscopic rectal cancer radical proctectomy, which is safe and feasible.

Objective To investigate effect of laparoscope anesthesia inducted by sevoflurane combined with different doses of propofol on vascular endothelial growth factor-C (VEGF-C) and prostaglandin E2(PGE2)in patients with hysteromyoma.Methods 77 cases of patients with uterine fibroids were selected and divided into two groups.40 cases in group A were anesthetized inducted by 2.0 mg/kg propofol combined with sevoflurane, 37cases in group A were anesthesia inducted by 1.0 mg/kg propofol combined with sevoflurane.VEGF-C and PGE2 in serum were compared before and 48h after the operation.Results Compared with preoperation, VEGF-C and PGE2in serum were lower (P<0.05), VAS score was lower (P<0.05);Compared with group B, VEGF-C and PGE2 in serum of the group A were lower (P<0.05), VAS score was higher (P<0.05).Conclusion Different doses of propofol on serum VEGF-C and PGE2 in patients with laparoscopic uterine fibroids have different effects, 1.0 mg/kg has a sedative effect, 2 0 mg/kg with anesthesia induction.

Objective: To study the expressien and heterogeneity of epidenmoid growth factor receptor(EGFR) in human mucoepidermoid carcinoma MEC 1 cells. Methods: EGFR1 McAb (MaH) immunofluorescent stain and flowcytometer (FCM) were used to detect the positive percentage and heterogeneity of EGFR expression in MEC 1 cells. Results: Positive expression of EGFR was found in 100% of MEC 1 cells. Average peak channel of relative fluorescent intensity was 8.006?4.410. Double peaks of MEC 1 cells from single parameter histogram was observed with FCM. Conclusion: All MEC 1 cells express EGFR and there are heterogeneous cell populations of EGFR expression in MEC 1 cell line.

Objective: To observe the interface bonding between the coral and new bone. Methods: The block coral was implanted on rabbit calvaria subperiosteally. The specimens were observed by the scanning electron microscopy 2, 4, 8, 12 weeks after operation respectively. Results: The coral was progressively absorbed and replaced by new bone. The coral bond to bone directly. No intervening layer was found between coral and bone. Conclusion: The coral bond to bone by direct mechanical way. [