Objective To explore the regulating mechanism of inducible nitric oxide synthase(iNOS) in hepatic injury of obstructive jaundice (OJ) in vivo and in vitro experiments. Methods (1) Rat hepatocytes were isolated by in situ collagenase perfusion and primary culture. Hepatocytes were pretreated with various concentrations of iNOS inhibitor SMT for 20 min. After pretreatment, 50?M GCDC was added for an additional 24hr. Cells were next detected by FCM and TUNEL.(2) Experimental obstructive jaundice (BDL) was induced by double ligation of the bile duct in rats. After BDL for 3d、7d、14d、and 21d, the apoptotic status in liver of all rats were determined with TUNEL, and iNOS protein in liver of OJ was ditermined with immunohistochemistry method. Results (1) SMT decreased GCDC-induced apoptosis in a concentration-dependent manner. (2) The apoptotic rate of liver was related to length of time of OJ. Apoptosis index (AI) was highest from rats with 14d bile duct ligation. The stronger the iNOS expression, the higher was the number of apoptotic cells that was found in OJ. Conclusions iNOS is involved in the regulation and the occurrence and progression of hepatic injury of obstructive jaundice.

This study was aimed to establish a HPLC fingerprint of saponins in Sanjie-zhentong Capsule in order to make a quantitative analysis of the quality of Sanjie-zhentong Capsule. The Waters Symmetry ShieldTM RP18 (4.6 mmí 250 mm, 5 μm) column was used with a mobile phase of acetonitrile-water gradient elution. The flow rate was 1.2 mL/min. The column temperature was 30℃. The detection wavelength was 203 nm. The results showed that the fingerprint chromatography included 9 mutual peaks. The similarity among batches was more than 0.95. Compared with reference substance, five characteristic components were recognized. The five components are notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ginsenoside Rd. It was concluded that this method was rapid, simple and accurate and can be used as one of the effective methods for the quality control of Sanjie-zhen-tong Capsule.

Objective:To develop a method for the quantitative determination of ambrisentan. Methods: 1 H NMR spectra were obtalned with a Bruker AscendTM 400 superconducting NMR spectrometer. For each sample, DMSO-D6 was used as the solvent, the pulse width was 10. 0 μs, the delay time was 5 s and the scanning time was 16. Results: The proton peaks of ambrisentan at δ6. 16 ppm and maleic acid atδ6. 28 ppm were used as the quantitative peaks. The linear regression equation of peak area and quality ratio was Y=0. 140 7X+0. 034 8 with the correlation coefficient of 0. 999 4. RSD was 0. 2%(n=6)in the repeated experiments. The absolute content of ambrisentan reference substance was 99. 9%. Conclusion: The results showed that 1 H NMR can be used in the quantitative determination of ambrisentan without reference substance. The method is reliable, rapid, accurate and simple.

Objective To study the effect of magnetic nanoparticles on human cholangiocarcinoma xenograft in nude mice, and compared with otherchemotherapy drugs Methods We established human cholangiocarcinoma xenograft in nude mice with QBC939 cell line.The nude mice were devided into 4 groups randomly.Saline,5-FU, Gemcitabine and magnetic nanoparticles were given to nude mice through tail vein on 20d after implanting QBC939 cell line. Calculations were done at different time after treatment in order to compare tumor volume,inhibition ratio of tumor and tumor growth curve of each group. The nude mice were killed on 35d after treatment to harvest tissue for electron microscopic examination to observe ultra-structural changes. Results The tumor volume of control, 5-FU, magnetic nanoparticles and Gemcitabine groups was (2256.1?267.1) mm3, (2096.5?237.9)mm3,(1392.2?189)mm3, and (1534.9?115 )mm3 respectively.The last two groups have significant difference compared to the first two groups(P

Objective:To discuss the changes in the levels of chemokine ligand 19(CCL19)and soluble CD163(sCD163)in serum of the patients with systemic lupus erythematosus(SLE)during pregnancy,and to clarify their effects on the maternal and infant outcomes.Methods:A total of 180 pregnant SLE patients were selected as SLE group and then divided into successful pregnancy group(n=132)and pregnancy failure group(n=48)based on the maternal and infant outcomes.A total of 180 healthy pregnant women underwent prenatal checks during the same period were randomly selected as control group.The general data of the patients in two groups were collected,and the serum levels of CCL19 and sCD163,along with related serum factors,were detected by kits.Multivariate Logistic regression analysis was used to detect the risk factors for pregnancy failure in the SLE patients,and receiver operating characteristic(ROC)curve was used to evaluate the effectiveness of serum CCL19 and sCD163 levels in predicting the pregnancy outcomes of the patients in SLE group.Results:Compared with control group,the levels of complements C3 and C4 in the serum of the patients in SLE group were significantly decreased(P<0.05),and the levels of erythrocyte sedimentation rate(ESR),creatinine(CR),anti-cardiolipin antibody(ACA)-IgG,anti-β2 glycoprotein Ⅰ(anti-β2GPⅠ),CCL19,and sCD163 of the patients were significantly increased(P<0.01).Compared with successful pregnancy group,the levels of complement C3 and C4 pregnancy of the patients in failure group were significantly decreased(P<0.01),and the levels of ESR,CR,ACA-IgG,anti-β2GPⅠ,CCL19,and sCD163 were significantly increased(P<0.01).The serum levels of CCL19,sCD163,ESR,CR,ACA-IgG,and anti-β2GPⅠ were the risk factors for pregnancy failure of the SLE patients(P<0.05 or P<0.01),while the levels of complement C3 and C4 were the protective factors(P<0.01).The area under the ROC curve(AUC)of the serum CCL19 level for predicting the pregnancy failure of the SLE patients was 0.726,and the AUC of serum SCD163 level for predicting the pregnancy failure of the SLE patients was 0.789;the AUC of combination of both markers for predicting the pregnancy failure of the SLE patients was 0.835.The predictive performance of CCL19 and sCD163 for pregnancy outcomes of the SLE patients was superior to either marker alone(Zcombined-CCL19=3.066,P=0.002;Zcombined-sCD163=2.087,P=0.037).Conclusion:The serum levels of CCL19 and sCD163 in the SLE patients during pregnancy are significantly increased,which may cause the poor outcomes in the patients.