Objective:To discuss the clinical value of CT-guided interstitial implantation of125I radioactive particle through the percutaneous puncture in the treatment of central-type lung cancer.Methods: 63 patients with the central-type lung cancer treated in Renmin Hospital of Wuhan University from November 2013 to July 2016 were randomly grouped (31 cases in the control group and 32 cases in the observation group). Patients in the control group were given the treatment of bronchial arterial perfusion with the chemotherapeutic drugs; while patients in the observation group were given the CT-guided interstitial implantation of125I radioactive particle through the percutaneous puncture. The clinical therapeutic effect and complications were observed of the two groups and the quality of life scores before and after the treatment were summarized between two groups as well.Results: The overall effective rate of observation group was 84.38% (27/32) that was higher than 61.29%(19/31) of control group, with statistical difference (x2=4.259,P<0.05); the complication rate of observation group was 9.38% (3/32) that was lower than 12.90% (4/31) of control group, with no statistical difference (x2=0.002,P>0.05); the quality of life score of observation group after the treatment was 84.57±3.27 that was higher than 70.38±3.20 of control group, with statistical difference (t=17.402,P<0.05).Conclusion: CT-guided interstitial implantation of125I radioactive particle through the percutaneous puncture in the treatment of patients with central-type lung cancer has the significant effect, and it can effectively improve the quality of life for patients and have the high degree of safety.

RNA interference(RNAi)is a post-transcriptional gene-silencing phenomenon which is triggered by double-stranded RNA(dsRNA).Attempts age being made to exploit RNAi in a clinical setting.However,before RNAi can be exploited as therepeutically,several obstacles must be overcome such as the efficient and safe delivery of siRNA into target tissues and cells.Any usefull delivery method should be desinged to target siRNA.

SNP may play an important role in the mechanism of sepsis. Detection SNP of the patients with sepsis may be the key point to the individualization treatment in near future.

Coal workers' pneumoconiosis（CWP）is one of the most common occupational diseases. The pathogenesis of CWP remains unclear and effective therapeutic drug is not exist. Therefore，looking for CWP-related biomarkers have become a focus of research. In recent years，intensive studies have been carried out on the pathogenesis of CWP，such as inflammatory response，alveolar epithelial cell injury，extracellular matrix remodeling，epigenetics，oxidative stress and immune dysfunction. It has been found that some biomarkers were related to CWP，such as high mobility group protein 1，nucleotide-binding oligomerization domain-like receptor family protein 3，surfactant protein，mucoprotein 5B，osteopontin，aminin，DNA methylation，microRNA，long noncoding RNA，cytochrome b-245-alpha polypeptide and cluster of differentiation，and others. These biomarkers are helpful for early screening，monitoring efficacy and pathogenesis research of CWP. However，it is necessary to further explore biomarkers with high sensitivity and specificity and conduct prospective clinical value evaluation to better guide the prevention and treatment of CWP .

Objective: Using MB-80 Microbiology Kinetic Rapid Reader, the methods were established to quantitatively measure the body fluid content of bacterial endotoxin and fungus in infectious patients. Methods: The blood samples were collected from Gram negative bacterial infection and deep mycosis patients and healthy volunteers. After pre-treatment, the plasma samples were measured using MB-80 system through kinetic turbidimetric limulus test method. The system computer could calculate the content of endotoxin and fungus during 1 hour detection. Results: The detection methods of endotoxin and fungus using MB-80 were simple and rapid. The process of pre-treatment was standardized and the results had good repeatability. The normal ranges of plasma endotoxin and fungus of human healthy volunteers were (2.03?2.32)ng/L and (2.46?2.15)ng/L respectively. The plasma endotoxin level of abdominal infection patients was increased significantly to 50-3 958 ng/L. The fungus level of deep mycosis patients was enhanced to 20-2 381 ng/L. Conclusion: The methods of endotoxin and fungus quantitatively measurement by MB-80 are simple and convenient and have results with good repeatability. The new detection methods of endotoxin and fungus can be used for diagnosis of endotoxemia and deep mycosis in early stage of infection.

So far, the method of detection of patent foramen ovale (PFO) by transcranial Doppler (TCD) has had two decades of history. Standardization in contrast-enhanced transcranial Doppler US procedures for cardiac right-to-left shunt detection was proposed in the Venice Consensus Paper in 1999, and some questions were raised at the same time. Over the past 10 years, the researchers have conducted a more in depth study on the method. This article reviews the related research progress.

According to the traditional point of view,CT and lumabar puncture are the main means in the diagnosis of subarachnoid hemorrhage(SAH).The values of conventional MRI T1-weighted imaging(T1WI)and T2 weighted imaging(T2WI)are very little in the diagnosis of SAH.With the development of MRI technique and the research and application of novel MRI sequences,the value of MRI are gradually recognized and affirmed in the diagnosis of SAH.and has shown its superiority.MRI isn't inferior to CT in the diagnosis of acute SAH.evidently superior to CT in the diagnosis of subacute,chronic and atypical SAHs and superior to CT in the diagnosis of the complications of SAH.MRI is valuable in screening for intraeranial aneurysms and postoperative follow-up.

The expression of c-erbB-1 and c-erbB-2 oncoproteins were investigated by immunohistochemical technique using monoclonal antibodies to c-erbB-1 and c-erbB-2 oncoproteins in 43 cases of hasal cell carcinoma (BCC) and 26 cases of squamous cell carcinoma (SCC). The results showed that: ① The expression of c-erbB-1 oncoprotein in BCC increased; the expression of c-erbB-2 oncoprotein in BCC reduced significantly, and lost in 16 cases (16/43). ② The intensities of c-erbB-1 and c-erbB-2 oncoprorein expression had negative and positive correlations to the degrees of SCC differentiation respectively. It is suggested that the abnormal expression of c-erbB-1 and c-erbB-2 oncoprotein in BCC and SCC may play a role in the development of skin tumors.

Objective To evaluate the clinical results and the value of usefulness with the operation methods of posterolateral transpedicular approach in treating thoracic disc herniation. Methods Selecting the patient who treated in our hospital from November 1999 to November 2003. In all the 45 patients who suffered from thoracic disc herniation, we had got 43 patients followed up, the following-up times were from 1.2 to 3.5 years. 38 patients were treated with posterolateral unilateral transpedicular approach operation method. There were 26 males and other 19 females. The patients were from 30.5 years old to 67.5 years old, and the mean age was 46.5 years old. The interval between onset of symptoms and operation ranged from 5 to 12 months, and 9 months for average. In this group, 45% patients were involved in T9-10 and T10-11 disc herniation, 4 cases(11%) in T8-9, 4 cases(11%) in T11-12, and 4 cases(11%) in T12L1. All patients underwent X-ray and MR examination. 22 patients underwent myelography, 25 patients underwent CT or CTM examination. Using Otanni scored system to evaluate the clinical results. Selecting the SPSS 11.5 to do the statistics works. Results A perfect result was found in 16 patients, a good result was found in a further 18 patients, and a fair result was found in 2 patients the outcome was unchanged with no effect in 2 cases. No clinical or radiographic features of instability, nervous symptoms and wound infection were found. The clinical satisfaction rate was 89.74%, the results showed that it was a good result of posterolateral entrance operation. Conclusion The posterolateral transpedicular entrance operation shows a good result in treating patients who suffered from thoracic disc herniation.

Objectives:To study the alterations of NF-?B activation in PBMC, in order to clarify the signal transcription of NF-?B concerned in the mechanism of inflammatory reaction in severe burns. To evaluate the alterations of expression of NF-?B dependent proinflammatory cytokines mRNA. Thereby, to observe the regulating effects of NAC on NF-?B, and verify the medial effects of upstream signal molecules on cytokines expression. Methods:Sprague Dawley rats were randomly assigned to each group. A 30% TBSA full-thickness scald was performed by immersing in 100℃ water for 12 seconds. PBMC were isolated at different time point after scalding. From isolated PBMC, the total RNA was isolated and the nuclear protein purified by Trizol reagents. The NF-?B proteins were measured through EMSA. Message RNA expression of proinflammatory cytokines and Th2 cytokines were assayed by RT-PCR. Results:Lots of NF-?B proteins were sequester in nucleus in all burn groups. mRNA expression of proinflammatory cytokines were also enhanced and related to the activity of NF-?B protein in nucleus. NAC could decrease the activity of NF-?B protein in nucleus. NAC had also significantly inhibitory effects on the production of proinflammatory cytokines. However, all of Th2 cytokines measured in this study were enhanced too. Conclusions:NF-?B might be the second signal molecule from cytosol to nucleus and mediate the proinflammatory cytokines transcription in PBMC after burns. In signal transduction levels, enhancing scavengers of oxygen free radicals in PBMC may modulate the activity of NF-?B which mediate the expression of proinflammtory cytokines.