Objective: To investigate the influence of polysaccharide of Spirulina platensis(PSP) on inhibition activity of immobilized phycocyanin (PC) against proliferation of pancreatic cancer cells SW1990. Methods: PSP was added to the wells of cell culture polystyrene which was immobilized with PC, and SW1990 cells were cultured on this kind of biomaterial in serum free medium. Results: When the concentration of PSP was 15 mg/L, the inhibition activity was mainly caused by PSP, and when the concentrations were 10, 17.5 and 25 mg/L, PSP was cooperative with immobilized PC. The best immobilized amount of PC was 0.02 mg/well, which could inhibit 86% SW1990 cells. Conclusion: PSP can enhance the inhibition activity against pancreatic cancer cells SW1990 from immobilized PC.

Polysaccharide is used for adjuvant immunotherapy of malignant diseases.We studied the potential applicability of Selenium polysaccharide (SePS) from Spirulina platensis for improving immune function using an experimental model of murine lymphoma,compared with polysaccharide (PS) in Spirulina platensis. SePS and PS have no effect on thymus index;spleen cell proliferation was heightened by high concentrations of SePS and PS;synergism of SePS,PS with ConA is significant.SePS treatment enhance the ability of natural killer cell in mice spleen to kill target cell of YAC 1;conclusively,SePS treatment is more significant in the improvement of immuofunction than that of PS.

Objective: To study and compare the views on cancer associated information and communication needs of cancer patients among cancer patients, their relatives, doctors and nurses, and other groups of people. Methods: A total of 501 cancer patients, 169 relatives,86 doctors and nurses, 176 healthy elderly people and 505 first grade college stu- dents were asked to complete the Information Preference Questionnaire of Cancer Patients and 2 questions regarding gen- eral information amount and content (IPQCP+2), and the Information Need Questionnaire (INQ). The elderly and the stu- dents were asked to complete two sets of IPQCP+2 and INQ in two roles: one as themselves and the other as patients. All data were analyzed in detail. Results: The views of different groups are significantly different. The differences include the viewpoints on whether to disclose the diagnosis of cancer and to inform the life expectancy to cancer patients, and whether the patients should participate in decisions about their medical care and treatments. The mean score of IPQCP on the over- all items and the 4 dimensions of all groups, and their opinions on general information amount and content for patients are significantly different. Conclusion: The views of different groups of people on cancer associated information and commu- nication needs of cancer patients are different. The views and requests of cancer patients themselves are the basis of doc- tor- patient communication. The prevention of cancer should be based on the characteristics of different groups of people and the patients’views should be considered.

Objective and Methods:The scavenging effects of the extracts, phycocyanin and polysaccharose from Spirulina platensis (S.P) or from selenium enriched S.P (Se-S.P), and sodium _ selenite on superoxide anions( O2? )and hydroxyl radicals (?OH) were investigated respectively with chemoluminescence technique. Results:The extract, Se-phycocyanin and Se-polysaccharose from Se-S.P had higher scavenging effect on both kinds of active oxygen radicals than those from S.P and the scavenging _ rates of extract on O2 ? and ?OH were enhanced about 30%and 20% at a concentration of 400?g/ml.Those effects of Se-phycocyanin were characterized dose-dependently and the concentration of 50% scavenging rate on superoxide anions was above 500 ?g/ml. With increase of the concentration of Se-phycocyanin, the scavenging rate on ?OH was elevated steadily and 50% scavenging rate was found at protein concentration above 125?g/ml. The scavenging effect of the Se-polysaccharides on oxygen free radicals was more _ noticeable and the concentration for 50% scavenging rate on ?OH and O2 ? were 60 ?g/ml and 12 ?g/ml _ respectively. However, there was no evidenced scavenging effect of sodium selenite on both ?OH and O2 ? . Conclusion : The active organic Se-containing molecules such as Se-containing proteins and Se polysaccharides, which were produced by selenium metabolism in microalgae, might contribute to the scavenging effects of Se enriched S.P. on oxygen free radicals.

Histamine is one of the important mediators of many diseases such as hypersensitivity, gastric ulcer, and inflammation. It regulates the local immune response in tumor tissues by multiple mechanisms with ambivalent and double-faced effects, which have been uncovered partially by thorough molecular immunological analyses. Histamine has differential effects on the growth of tumor cell depending on selective reaction with its receptors, and on the secretion of variety of cytokines from local activated immune cells in a reciprocal manner by shifting TH1/TH2 polarization towards predominance of TH2. In this review, we summarized recent data suggesting that endogenous histamine should be an important correlated factor involved in bi-directional regulation both to tumor tissue and to infiltrating immune cells.

The bioactive protein-phycocyanin and all the proteins of Spirulina Platensis were isolated and purified. Photo-reactive proteins were synthesized by coupling the proteins with (N-(4-azidobenzoyloxy)succinimide) and were spread onto the 24-well cell culture polystyrene plate. Then the coated surface was exposed to ultraviolet irradiation for chemical fixation of proteins via the conversion of the phenylazido group to the highly reactive phenyl-nitrene which spontaneously formed covalent bonds with neighboring hydrocarbons. On these proteins-immobilized polystyrene plates, the liver cancer cells 7402 were cultured under the serum-free conditions, and the inhibition activity on proliferation of liver cancer cells was investigated and analyzed.
Animals ; Bacterial Proteins ; chemistry ; pharmacology ; Biocompatible Materials ; pharmacology ; Cell Division ; drug effects ; Cyanobacteria ; chemistry ; Liver Neoplasms ; pathology ; Photochemistry ; Polystyrenes ; chemistry ; Spirulina ; Succinimides ; chemistry ; Tumor Cells, Cultured

ObjectiveTo explore the effect of precocious puberty on glucose metabolism and lipid metabolism in female rats. MethodsSixty two-day-old female rats were randomly divided into 2 groups. When aged 5 days， the precocious puberty group and normal group were given a single subcutaneous injection of danazol and solvent soybean oil respectively. The vaginal opening of rats was monitored from their 21 days of age. After 12 hours of fasting， all successful modeling rats were randomly executed within 3 days after vaginal opening， when aged 7 and 12 weeks. Then we measured the rats’ body weight and length， determined the concentrations of glucose， insulin， blood lipids， estradiol， leptin and adiponectin with enzyme-linked immunosorbent assay and observed the pathological changes of perirenal fat， uterus and ovary. ResultsFor body weight and length， rats in the precocious puberty group were smaller than those in the normal group within 3 days after vaginal opening， but which did not affect their subsequent growth and development， and there was no significant difference between the two groups at 7 and 12 weeks of age. Within 3 days after vaginal opening， insulin levels had significant difference between the two groups （P = 0.001）， the precocious group showed hyperinsulinemia and increased number of perirenal adipocytes. At three execution times， no significant difference was noted in estradiol， leptin and adiponectin levels between the two groups. The same was true in the ratios of ovary or uterus to body weight between the two groups. ConclusionsPrecocious puberty makes earlier onset of pubertal development and allows body maladaptation to the sudden changes of the internal environment. However， the changes due to precocious puberty are temporary and reversible， and they may become normal in adulthood.