At present, the administration about putin to output of intrinsic economical running in legionary hospitals is being improved constantly. Therefore, we should firmly grasp "the three key strongpoints" of financial administration of basic construction: First make final check on the demonstrating about item feasibility; The second, make final check on the planning about protocol of item inviting bids; The third, make final check on the auditing about item outlay plan.

Objective To explore the efficacy of case analysis and problem discussion in teaching the course of introduction to clinical medicine for law majors and to discuss their influence on cultivating students' ability and quality.Methods Totally 58 students were randomly divided into three groups:trial group A(n =20),trial group B (n =16) and control group (n =22).Trial group A was further randomly divided into six groups.Teaching contents included iron deficiency anemia and acute leukemia.Trial group A and trial group B adpoted case analysis and problem discussion while control group practiced traditional classroom teaching.Results of theoretical test were processed through one-way analysis of variance and the least significant difference,using SPSS 11.5 software.Self-evaluation of teaching effects on students'specialized learning,logic thinking and knowledge of diseases were analyzed by Chi-square test and Fisher's exact test.Influences of different teaching methods on self learning ability,summarizing ability,team cooperation consciousness and lateral thinking were evaluated after lecture.Results Exam results showed no difference between trial group A and control group (P =0.564),the students in trial group A had a significant higher score than those in trial group B (P =0.004).100％ and 75.0％ students in trial group A considered the course of introduction to clinical medicine helpful concerning knowledge of diseases and students' specialized learning,higher than those of 77.3％ and 40.9％ in control group,with statistical differences(P =0.049,P =0.026).Positive evaluation rates of self learning ability,summarizing ability,team cooperation consciousness and lateral thinking were 100％,70.0％,80.0％,60.0％ in trial group A ;75.0％,68.8％,62.5％,81.3％ in trial group B;22.7％,36.4％,4.5％,27.3％ in control group.Except for summarizing ability,students in trial group A and B were significantly better than those in control group(P＜0.05).Conclusions The teaching method of case analysis and problem discussion meets the needs of versatile legal personnel training and it can stimulate students' study interest as well as improve their professional quality and ability.

Objective To examine the effects of fixed appliance and micro-implant on the improvement of facial profile in patients with protruding.Methods 30 protruding cases were treated by fixed appliance and micro-implant.Cephalometric data before and after treatment were measured and analyzed to assess the effects.Results The upper incisors were retracted 6.5 mm.The upper lip was retracted 3.8 mm.The lower incisors were retracted 5.8 mm.The lower lip was retracted 5.6 mm.UL-E was retracted 3.21 mm.LL-E was retracted 3.67 mm.NLA was changed from 86.7°to 103.7°.The U1-SN,U1-Y,L1-MP,L1-Y,UL-Y,LL-Y,UL-E,LL-E,and NLA demonstrated significant differences between pre-and post-treatment.Conclusions Fixed appliance and micro-implant might retract the anterior teeth and lead to an improvement of facial profile.

OBJECTIVE:To prepare oxymatrine pellets and study the pellets' properties.METHODS:Oxymatrine pellets were prepared using an experimental low-temperature extrusion-spheronization granulator.L9(34)orthogonal design was used to obtain optimal formulation.The micromeritic properties and in vitro dissolution of the pellets at different dosage were determined.RESULTS:Oxymatrine pellets prepared by extrusion-spheronization were round and smooth and well-distributed.The optimal technical conditions were as follows:water∶MCC=0.90∶1;spheronization velocity=35 Hz;spheroniza-tion time=5 min;extrusion velocity=40 Hz.The in vitro dissolution was more than 75% within 30 minutes.CONCLUSION:The process of preparing pellets by extrusion-spheronization was simple and feasible and the quality of pellets was excellent.

The paper described the necessity of the doctor-patient information exchange platform,and defects found in existing platforms. Such a platform is conducive to higher hospital informationization, better patient satisfaction, and better social and economic benefits of the hospital.The hospital may improve its quality of care and patient satisfaction by means of the platform

Objective To discuss the pathogeny,treatment and prophylactic measures of postcholecystectomy syndrome (PCS).Methods The clinical data of 150 patients with laparoscopic PCS in our department from October 2000 to March 2009 were analyzed.Results Etiological factors were found in 131 patients:one hundred and twelve cases were due to the reasons of biliary system,including bile duct residual stones after cystic resection,the injury bile duct stenosis,a long residual cystic canal,nipple benign stricture,bile duct tumor etc;Nineteen examples were due to other reasons,including gallbladder stone merger reflux gastritis,gastroduodenal ulcer,diverticulum beside duodenal nipple,and so on,which resulted in the symptoms un-release after cystic resection.Nineteen cases were not found organic lesion.In ones whose etiological factors were definite,117 cases were treated with different surgeries according to different etiological factors;another 33 cases were treated with conservative treatment.Total 145 cases were followed up,and 139 cases in them were cured or relieved at different degrees.Conclusion Careful preoperative examination,normalized operation avoiding damaging bile duct and leaving behind bile duct stones can effectively prevent laparoscopic PCS.

OBJECTIVE: To prepare Oxymatrine enteric-coated pellets.METHODS: The coating conditions such as the rotation speed,the coating temperature as well as the pressure of spray gun were optimized through single factor experiment;the properties of different coating solutions of resins were compared to optimize the coating material.The enteric-coated pellets were prepared by bed coating technology.The coating effect was evaluated by the dissolution in vitro. RESULTS: The optimal coating conditions were as follows: the rotation speed of the coating pan was 50 r?min-1,the coating temperature was 30 ℃,the pressure of spray gun was 0.10 MPa;the coating material was polyacrylic resin(Huzhou Ⅲ).The in vitro dissolution of the enteric-coated pellets prepared by the bed coating technology met the requirements specified in the Chinese Pharmacopeia(2005 edition).CONCLUSION: By using the bed coating technology,the enteric-coated pellets with good dissolution in vitro can be obtained.

AIM: To prepare flexible nanoliposomes made from active ingredients,phillyrin and volatile oil,from forsythia suspense and study their transdermal delivery system.METHODS: Flexible nanolipsomes of phill-yrin(WN group) and phillyrin in combination with forsythia volatile oil(OWN group) were prepared by the meth-od of membrane-dispersion.Its appearance and particle sizes were measured.Transdermal experiments were carried out on the modified Franz diffusion pool through in vitro mouse skin.HPLC was applied to determining the phillyrin content to compare transdermal rate and cumulative permeation amount of various flexible nanoliposomes.RESULTS: The particle size of the WN group was(180.7 ? 13.69)nm,the Zata potential was-48.8 mV,the average encapsulation percentage was(82.53 ? 2.68)%;the particle size of the OWN group was(212.3 ? 15.31)nm,Zata potential was-51.2 mV,the average encapsulation percentage was(70.49 ? 1.06)%.The accu-mulated permeation amount of the OWN group in 8 hours was(291.92 ? 23.22) ?g/cm2,its transdermal permea-bility in 8 hours was 36.49 ?g/(cm2.h),which was 6.10 folds that of the WS group and 1.92 folds that of the WN group.This difference had statistical significance(P

Objective Using the effect of microneedle array to investigate the feature and rule of transdermal drug delivery of sinomenine hydrogel patch.Methods The microneedles with different length and sinomenine hydrogel patch were prepared;The isolated rat skin was pretreated by different needle-shapes of microneedle array and different timepoints with the same needle-shape,and then sticked the sinomenine patch.The permeation rates of sinomenine were studied using a Franz diffusion cell and compared with passive diffusion of the rat skin untreated.Sinomenine content was measured by HPLC.Results The permeation rates of sinomenine via the skin pretreated with 100 and 200 ?m microneedles were 40.7 and 52.4 times to those of the untreated.The permeation rates of sinomenine via the skin pretreated 7 min with 200 ?m microneedles is 142.0 times to those of the untreated.When skins were pretreated using 200 ?m microneedles with different force, the drug permeation rates were increased with the force increasing.When the force exceeded 5 N,the drug permeation rates were equilibrium.Conclusion When transdermal drug delivery by using microneedle array-hydrogel patch,the permeation rates increase significantly.The needle-shape,skin pretreating time,and force of the microneedles play the important roles in the transdermal drug delivery.

Objective To study the ultrastructure of human oocytes cultured in vitro.Methods Ultrastructure of oocytes at different stages and of different quality were observed under transmission electron microscope(TEM).Immature oocytes at germinal vesicle(GV) stage and at MI stage after germinal vesicle break-down before and after being cultured in vitro,as well as mature oocytes at MⅡ stage after being cultured were evaluated according to their morphology under light microscope,and then studied by TEM. Results At GV stage the microvilli on oocyte surface were rare and shorter.Nucleus with a dense nucleolus was located in the periphery of oocyte.After culture the oocyte microvilli became denser and increased in number.There were rich ooplasm with organelles,such as large numbers of secretion vesicles,mitochondria and lipid droplets in the GV oocyte.The end of cumulus cell expanded and penetrated into the oocyte zone,forming gap junctions with oocyte microvilli.Functional connection of the adjacent cumulus cells was by desmosomes. In mature oocytes the rough endoplasmic reticulum and Golgi apparatus decreased while the smooth endoplasmic reticulum increased in number.Mitochondria degenerated and lipid droplets fused to form bigger ones.Cortical granules were distributed beneath the cytoplasm membrane in a line manner.The cell junctions between oocytes and cumulus cells were interrupted.There were various organelles in the first polar body.The mature oocytes with lower quality appeared to have lots of swollen mitochondria with blurred cristae and membrane.Some of these oocytes had fractured first polar bodies and deformed zona pellucida.Their surrounding cumulus cells showed higher frequency of cell apoptosis.Conclusion The ultrastructure of human oocytes at different stages and of different quality cultured in vitro was revealed.