ObjectiveTo study the method of testing abrasion resistance of axillary crutch tips. MethodsA test method based on pressure analysis in normal use of axillary crutches was suggested, which characterized: put 450 N on axis of the tip; the tip or text surface sway 60° for 500000 times in frequency of 40 /min; maximal abrasion thickness after test should be less than 3.3 mm. Experimental facilities were established to carry out fatigue test of wearability. ResultsThe test method suggested can reflect tips abrasion in real context. ConclusionThe text method can be used for tips abrasion and fatigue resistance adjustment.

The author presented an overview to the development of standardization of assistive devices for persons with disability in China and an analysis to the characteristics and existing problems in the field of standard of assistive devices. Thus the countermeasures on development of standardization of assistive devices in China were suggested.

The article introduced the concept and principles of universal design and discusses the strategies of research and development of universal design products based on the principles. The universal design benefits not only elder people and persons with disabilities, but also benefits other people without disabilities and the manufacturers of such products. The principles of universal design should be integrated in related policies and blueprints of future development in order to provide "reasonable accommodation" with universal design products to the widest possible range of people.

Objective:To study influence study of near and long term survival rate on different resection regions for patients with gastric cancer in M region.Methods:110 patients with M region gastric cancer received treatment in our hospital were divided into observation group(with total gastrectomy) 57 cases and control group (with subtotal gastrectomy) 53 cases,compared with clinical pathology data and radical degree,two groups of patients' operation index,and local recurrence rate and near and long term survival rate in the two groups.Results:Two groups of patients were compared with clinical pathology data and radical degree,the difference was not statistically significant (P＞0.05).The observation group's nearly cutting edge outside distance and lymph nodes were significantly higher than the control group,the difference was statistically significant (P＜0.05).There was no significant difference between the two groups(P＞0.05).The recurrence rate(3.51％) and recurrence rate (15.79％) of the observation group were significantly less than the control group of 16.98％ and 33.96％,and the difference was statistically significant (P＜0.05).The 1 and 3 year survival rates of the observation group were compared with the control group,the difference was not statistically significant (P＞0.05).But the 5 year survival rate of the observation group was 57.89％,which was significantly higher than that in the control group of 35.85％,the difference was statistically significant (P＜0.05).Conclusions There is no significant difference in the survival rate of total gastrectomy in patients with gastric cancer in M region and total gastrectomy,but the long-term survival rate is higher,it is worthy of clinical attention.

Objective To explore the effects of tamoxifen on the proliferation of SHG-44 glioma cells and the currents of sodium channel. Methods The cell activity was detected by MTT. The alteration of cellular proliferation and apoptosis were dectected by flow cytometer. Whole-cell patch clamp technique was used to record the Na currents.Results After treatment with tamoxifen,the cells began aging and shedding and cell counting decreased.The cells in G2/M cell cycle were more than that in control and the apoptosis ration increased. Tamoxifen significantly decreased the amplitude of Na currents of SHG-44 cell line.This blocking effect was dose-dependent and voltage-dependent.When the holding potential was 0 mV, 8 ?mol/L tamoxifen could block this currents by 69%.The half inhibition concentration(IC50) was 5.54 ?mol/L. Conclusion Tamoxifen can inhibit SHG-44 glioma cells proliferation.The inhibion of sodium channel may be one of its mechanisms.

Objective To explore the application of adopting the solid phase adsorption method to extract serum hepatitis C virus (HCV) RNA in the real-time fluorescence quantification PCR( RT-qPCR) by the improvement of solid phase adsorption method . Methods Firstly ,the surface of silica nano-particles was modified with Si-OH for increasing their adsorption ability with HCV RNA .Then the concentration of guanidinium thiocyanate (GuSCN) in the viral lysate was optimized .Thirdly ,the HCV RNA of standard serum samples was extracted using the modified solid-phase adsorption method and then performed in the RT-qPCR .Its standard curve was drawn and the linear equation was calculated .At the same time ,the lowest detection limit of RT-qPCR for de-tecting HCVRNA in serum sample was measured and the detection repeatability was verified .Results The adsorption ability of sil-ica nano-particles for HCV RNA in serum samples was remarkably improved after surface modification with Si-OH .The optimal GuSCN concentration in viral lysates was 4 .23 mol/L .Using the modified method to extract HCV RNA in serum samples was ap-plied in RT-qPCR ,the standard curve and the linear equation were obtained ,the linear correlation coefficient(R2) reached to 0 .999 and the detected linear range was (2 .52 -5 .52) IU/mL or 102 .52 -105 .52 pathogen number/mL .The detection limit of HCV RNA in serum samples was (2 .52 ± 0 .50)IU/mL with good detection repeatability .Conclusion The modified solid-phase adsorp-tion method can greatly decrease the lowest detection limit of RT-qPCR and increase the positive detection rate of HCV .This meth-od is simple to operate ,has low cost and can be widely applied in the HCV detection and the nucleic acid detection of other viruses in clinic .

Objective To evaluate the value of 18F-FDG PET in predicting the prognosis of pancreatic cancer. Methods Fifty-four cases of pancreatic cancer were divided into two groups. Taking standard uptake value(SUV) at 4 as the cut off point, patients (22 cases) with that not more than four were classified into group A, and those (32 cases) with SUV greater than four were into group B. The prognosis of patients by SUV was analyzed statistically. Results There was a statistically significant difference in survival between the two groups ( P =0. 01 ). The 1-,3-year survival rate was 68. 18% 、34. 91% in group A in those the SUV≤4, and 33.61% 、11.95% in group B( SUV ＞4), respectively. Cox proportional hazard model showed that tumor staging and SUV were the significantly independent prognostic factors in patients with pancreatic cancer. Conclusions 18F-FDG PET is of value in predicting the prognosis of patients with pancreatic cancer.

OBJECTIVE:To prepare oxymatrine pellets and study the pellets' properties.METHODS:Oxymatrine pellets were prepared using an experimental low-temperature extrusion-spheronization granulator.L9(34)orthogonal design was used to obtain optimal formulation.The micromeritic properties and in vitro dissolution of the pellets at different dosage were determined.RESULTS:Oxymatrine pellets prepared by extrusion-spheronization were round and smooth and well-distributed.The optimal technical conditions were as follows:water∶MCC=0.90∶1;spheronization velocity=35 Hz;spheroniza-tion time=5 min;extrusion velocity=40 Hz.The in vitro dissolution was more than 75% within 30 minutes.CONCLUSION:The process of preparing pellets by extrusion-spheronization was simple and feasible and the quality of pellets was excellent.

Objective:To compare the effects of distal movement of canines between elastic traction and canine lace back in the aligning stage of preadjusted appliance. Methods:50 cases with Angle I malocclusion were divided into two groups after first premolar extraction. Patients in both groups were treated by the same kind of preadjusted appliance in the aligning stage. Canine lace back was used in one group and elastic traction in another.Treatment was countinued for 12 weeks.Measurements of the dental models and the cephalograms were conducted before and after treatment.Results:Compared to the canine lace back, the decrease of the width between the cuspids of upper premolars and that between that of lower molars, the increase of the angle of the distally tipped canines and the mesially tipped lower molars and the lingually tipped incisors, the higher speed and larger distance of distal moving canines were achieved more easy with elastic traction. On the contrary, the larger distance of mesial moving molars were attained with canine lace back. The differences of all the measurements above between the two methods were significant(P

Aim To establish a resistant human osteosarcoma cell line(Saos-2/ADM1and Saos-2/ADM4)from the Saos-2 cell line and study its resistant mechanisms.Methods Saos-2 cells were pulse exposed in gradually increased dose of ADM culturemedium.The sensitivity of the resistance drug from the Saos-2、Saos-2/ADM1 and Saos-2/ADM4 cell lines to ADM、DDP、EPI、MTX、THP and PTX was measured by MTT assay.The morphology and ultramicro structure of the cell lines were observed by optical microscopy and transmission electronic microscopy.The expression level of MDR1 mRNA、MRP mRNA and their proteins P-gp、MRP was examined by reverse transcription polymerase chain reaction and immunohistochemistry.Results Resistance cell lines were established after 167 days.The resistance index to methotrexate of the Saos-2/ADM1and Saos-2/ADM4 cell lines to ADM was 49.8 and 74.6 times than that of Saos-2 respectively.The two cell lines had resistance to MTX、IFO、EPI、THP and PTX(P0.05).Disordered structure of the Saos-2/ADM1and Saos-2/ADM4 cells was observed through microscopy.The cells appeared in coenocytic.The decrease of cell villus and the increase of nucleoli were observed through transmission electronic microscopy.The proliferation ability of Saos-2/ADM1and Saos-2/ADM4 cells decreased significantly.MDR1 mRNA、MRP mRNA、P-gp and MRP showed positive staining in resistance cell lines.Conclusion The genes of MDR1 mRNA、MRP mRNA and their corresponding proteins participated in the formation of multidrug resistance in resistant adriamycin cell line.These newly-described resistant osteosarcoma cell lines were useful models for further characterization of drug resistance in osteosarcoma and for the development of treatment protocol.