MicroRNAs are a class of small(about 20-24 nueleotides),endogenous,noneoding RNA molecules that regulate a wide array of physiologic processes,such as growth and developmenL In recent years,some researches show that the tissues from tumors have aberrant mieroRNA profiling compared with normal tis-sues.The abnormal miemRNA profiling can affect the genesis,proliferation,differentiation,metastasis and recur-renee of tumors.The different expressions of microRNA Can not only help for the diagnosis of tumors,but also evaluate the prognosis of tumors.Disturbing several miemRNA expressions can decrease the volumes of tumor and inhibit the peritoneal metastasis 80 as to treat the tumor by using anti-mieroRNA drugs in animal expedments.

Delta-like ligand 4 (D114), one ligand of Notch receptors, was highly expressed in turnout vessels and was a negative regulator in tumour angiogenesis. Blocking D114/Notch signaling could promote tnmour angiogen-esis, whereas due to poor peffnsion of newly forming vessels, tumour growth was inhibited, which was effective even to the tumour patients unresponsive to blocking VEGF pathway. So, D114 is becoming a new promising target in anti-tumour therapies.

Delta-like ligand 4(Dll4), one ligand of Notch receptors, is highly expressed in tumour vessels and is negative regulator in tumour angiogenesis. Blocking Dll4/Notch sinaling could promote tumour angiogenesis, whereas due to poor perrusion of newly forming vessels, tumour growth is inhibited, which is effective even to the tumour patients unresponsive to blocking vascular endothelial growth factor pathway. So, Dll4 is becoming a new promising target in anti-tumour theraties.

The metabolic syndrome is a complex disorder of several metabolic abnormalities,which represents a risk factor of type 2 diabetes mellitus and cardiovascular diseases.Insulin resistance seems to play a significant role in pathoyenesis of metabolic syndrome.Many issues concerning definition,pathophysiology and optimal treatment of metabolic syndrome are reviewed in this article.

Objective To investigate the effect of blood pressure when measuring with clothing. Methods Measure the blood pressure of the right upper limb by using two different methods among the same group, and then analyze the data. Results Both SBp and DBp values which have measured with clothing are significant higher than those which have measured without clothing. The average difference of SBp and DBp are 1.54 mm Hg and 1.01 mm Hg respectively. Conclusion The patients who have no abnormal Bp history can be measured Bp with clothing, while this method should be forbidden to the patients who have hypertension or hypotension history.

Objective To investigate healthcare-associated infection(HAI)prevalence and antimicrobial use in a hospital.Methods HAI prevalence rate,antimicrobial use and pathogen detection in all inpatients on August 21 , 2013 were investigated with cross-sectional survey method.Results A total of 2 238 inpatients were investigated, 104 patients developed 126 times of HAI,HAI prevalence rate and case rate was 4.65% and 5.63% respectively;the top four sites for HAI were lower respiratory tract (28.57%),upper respiratory tract(18.25%),urinary tract (7.94%)and gastrointestinal tract(4.76%).Rate of specimens delivered for detection was 91 .35%(95/104),the main specimen was sputum (26.32%),followed by blood (25.26%)and urine (10.53%).Antimicrobial usage rate was 24.58%,therapeutic,prophylactic plus therapeutic,and prophylactic use accounted for 36.55%,45.09%, and 18.36% respectively;the usage rate of single,combination of 2,and 3 antimicrobial agents accounted for 75.82%,20.91 % and 3.27% respectively.Risk factors of HAI were age (<15 or >60 years),all kinds of inva-sive procedures (tracheotomy,mechanical ventilation,urinary catheterization,arteriovenous intubation,hemodialy-sis),and anti-tumor chemotherapy.Conclusion Survey on HIA prevalence helps to know the occurrence of HAI and usage of antimicrobial agents,as well as risk factors and high-risk departments of the occurrence of HAI,it also provides basis for subsequent targeted monitor on HAI.

0.05 ).Nitrogenous retentive rate,net nitrogenous utilizing rate and cumulative nitrogen balance were higher in Glamin group compared with those in Vamin group and Novamin group( P

Objective To detect bacterial content on surface of mobile phones of health care workers (HCWs)by adenosine triphosphate (ATP )bioluminescence assay.Methods HCWs in departments of internal medicine,surgery, medical laboratory,and administration were randomly selected,50 in each department,field detection on bacterial content on surface of mobile phones of HCWs was conducted,the relevant data were recorded.Results A total of 200 mobile phones were detected,33 mobile phone surface were qualified,the qualified rate was 16.50%.Qualified rate of mobile phone surface of HCWs in different departments as well as mobile phone disinfected by different modes were different(χ2 =13.46,10.24,respectively,both P <0.01);difference in qualified rate of mobile phones of different types,different service life,and different protective case were all not significant (χ2 =4.37,1 .87,0.25 respectively,all P >0.05).Conclusion The qualified rate of bacterial content on surface of HCWs’mobile phone is low,the awareness of hand hygiene of HCWs should be strengthened,regular cleaning and disinfection on the mo-bile phone can effectively reduce bacteria on the mobile phone surface.

BACKGROUND:Acelular matrix which is decelularized but retains the matrix components in the nature materials can reduce the immunogenicity of natural materials effectively and keep the material mechanical strength. OBJECTIVE:To prepare the natural biological scaffold material by using elution method to remove the cels in the rabbit costicartilage matrix. METHODS:After removal of the surrounding tissues, the costicartilage samples from New Zealand white rabbits were randomly divided them into three groups: costicartilages untreated as normal control group, detergent-enzyme digestion for 48 hours as 48-hour treatment group, detergent-enzyme digestion for 96 hours as 96-hour treatment group. Hematoxylin-eosin staining and electron microscope were used to observe decelularized effects. At 7 days of induction, bone marrow mesenchymal stem cels, 3×109/L, were colected and co-cultured with alogeneic acelular costicartilage matrixin vitro. At 3 and 7 days of co-culture, the composite was taken for observation of cel growth on the cel-free substrate surface under electron microscope. RESULTS AND CONCLUSION:Two or three cartilage cels were closely packed in each cartilage pit, began to depigment using the detergent-nuclease digestion method, and then completely depigmented at 96 hours after treatment. At 3 days of co-culture, there were a large amount of polygon-shaped adherent cels with pseudopodiasobserved on the acelular matrix surfacein vitro, and cels could proliferate and divide in partial regions. At 7 days of co-culture, adherent cels spread mostly throughout the acelular matrix surface, these cels were flat-shaped and extended with multiple interconnected processes. Mass of secreted excelular matrixes were deposited on the matrix surface and showed frost-like changes, indicating the prepared acelular matrix has favorable cytocompatibility.

Objective:To clarify the mechanism of immunoregulation of bone marrow stromal cells(BMSCs) transfected interleukin 18(IL-18) after their transplantation into glioma bearing rats.Methods:Cultured BMSCs from SD rats were transfected with rmIL-18(BMSCs/IL-18).Untransfected BMSCs were used as control.Culture supernatant medium was collected for IL-18 examination at different time point by ELISA kit.After establishment of glioma bearing rats followed by BMSCs/IL-18 transplantation,serum concentration of IFN-?,IL-2 and IL-10 were examined by means of ELISA kit.And their splenocytes were cultured with C6 cells and BMSCs/IL-18 for in vitro cytotoxicity assay,and subsets of splenocyte were detected by flow cytometry.TUNEL was used to clarify apoptosis cells inside glioma and anti-CD34 staining was performed to observe microvessel density(MVD).Results:BMSCs/IL-18 could secret IL-18 long term and stably.After being transplanted with BMSCs/IL-18,serum concentration of IL-2,IFN-? in glioma bearing rats' increased obviously and serum concentration of IL-10 decreased.Flow cytometry results showed that CD4+ and CD8+ T lymphocytes increased in the splenocytes.And rechallenge with C6 cells induced a rapid immuno-reaction.In vitro cytotoxicity assays.It was showed that BMSCs/IL-18 could stimulate splenocytes to kill C6 cells obviously.TUNEL assay showed that there were 15.74?6.23 apoptosis cells inside glioma in each view in Group 2,which was much more when compared with other groups.Microvessel density inside glioma in group 2(6.51?2.71) was lower than in group 1(13.52?3.06),group 3(12.67?2.61) and control group(14.84?1.47).Conclusion:By means of inducing Th1 cytokine and suppressing Th2 cytokine and activating cytotoxic T lymphocyte,BMSCs/IL-18 induces obviously anti-tumor activity.