Purpose:To explore the relationship between genetic polymorphisms of p53 codon72 and the risk of gastric cancer.Methods:A population based case control study was conducted in Taixing, Jiangsu Province, China, and 204 gastric cancer cases and 415 controls were collected. Results:Distribution of Pro/Pro genotype in cancer cases was significantly higher than controls. The OR and 95%CI of Pro/Pro or Pro/Arg genotype was 1.5 (1.01～2.23) compared with Arg/Arg genotype. Pro/Pro or Pro/Arg genotype may interact with cigarette smoking, alcohol drinking, river water drinking and high salt food intake. Conclusions:Pro genotype may be related with the risk of gastric cancer and may also interact with environmental risk factors to increase the risk of gastric cancer.

To perform modal analysis of CT shelter by applying computer simulation technology so as to pro-vide theoretical guidance for CT shelter structure optimization. Based on CAD model, the finite element model of a CT shelter was established with ANSYS simulation platform. Through modal analysis, different-order modal frequency and modal shape of the shelter were computed and the kinetic characteristics were evaluated. Low order modal frequency was kept away from the natural frequency range of chassis system resonance to avoid the overall structure reso-nance; the 3rd and the 4th modal frequency and engine idle speed frequency were very close so that local resonance might occur; road roughness excitation frequency covered the first 6 order modal frequencies and the further vibration-re-ducing measures of CT equipment were suggested. Based on the theories of finite element method and current software platform, modal analysis of shelter structure can be simulated and the results can provide valuable data for the improvement of kinetic characteristics and structure design.

Objective To investigate the safety and efficacy of mechanical anastomosis with Chinese staplers in the surgical treatment of esophageal carcinoma and carcinoma of gastric cardia in order to find out an alternative effective anastomotic technique to hand-sewn method. Results Compared with the reported postoperative leakage rate from 2%-4% in China and 4.2%-12.3% abroad, our techniques reduced the total leakage rate to 0.8%, that is, cervical leakage rate was 11.4% (5/44), and intrathoracic leakage rate 0.57% (11/1921). The intrathoracic leakage rate was 0.99% (8/808) for the first 8 years, and 0.27% for the following 8 years. There was no leakage (0/350) in the last 3 years. The anastomotic stricture occurred in 98 patients (4.99%) which could be relieved by Bougie or balloon dilatation. The total operative mortality was reduced to 0.76%.Conclusions Mechanical anastomosis with Chinese staplers combined with telescope suturing should be an effective method to prevent the occurrence of anastomotic leakage after esophageal resections.

Objective. To study the performance of free nerve graft with vascular implantation.Material and method. Three groups of rats were used. A 10 mm nerve defect was made in the sciatic nerve. It was repaired respectively with vascularised nerve graft(VNG), free nerve graft with vascular implantation(NGV) or free nerve graft(NG). Before 30 days the revascularisation of the nerve graft was assessed;at 2 to 3 month regeneration of nerve was evaluated. Histochemical staining, vascular morphology and electrophysiological examination were undertaken.Result and conclusion. The number of regenerated blood vessels in VNG and NGV was significantly increased than that in NG before 30 days. The ratio of regenerated nerve fiber area and conduction velocity in the first two groups were much better than those in NG at 2 to 3 months.

AIM:To explore the therapeutic effect of a novel Rho kinase inhibitor WAR 5 on the experimental autoimmune encephalomyelitis (EAE) and its possible mechanism.METHODS: Female C57BL/6 mice were randomly divided into EAE group and WAR5 group.EAE model was induced by the application of MOG 35-55 peptide.WAR5 was in-jected intraperitoneally every other day from post-immunization (PI) day 3 to PI day 27.The clinical score and body weight were recorded every other day .On PI day 28, the animals were sacrificed and spinal cords were obtained for HE and mye-lin staining .The splenocytes were isolated and the expression of CD 16/32 and CD206 were analyzed by flow cytometry . The protein extracts from the brains and spinal cords were collected for the measurement of inducible nitric oxide synthase ( iNOS) by Western blotting .RESULTS:The administration of WAR 5 delayed the onset of EAE and attenuated the clini-cal symptoms .The results of the pathological examination revealed that WAR 5 inhibited the infiltration of inflammatory cells and improved myelination in spinal cords , accompanied with the poralization of M 1 macrophages to M2 phenotype in the spleen.WAR5 inhibited the expression of iNOS in the central nervous system , especially in the spinal cords .CON-CLUSION:The therapeutic effect of WAR5 on EAE may be related to the shift of M1 macrophages to M2 phenotype and inhibition of inflammation in the central nerve system .