Objective:To compare the differences of cranial base morphology between high-angle and low-angle malocclusion patients with geometric morphometrics.Methods:Pretreatment lateral cephalograps of 80 skeletal Class I patients(40 hypodivergent,40 hyperdivergent)were selected.All cephalographs were scanned,and 13 homologous landmarks of cranial base were identified and digitized.Average geometries of cranial bas e were created by means of Procrustes analysis.Thin-plate spline analysis was applied to cranial base configurations to determine local morphology differences between the two vertical facial types.Results:1.high-angle patients had the cranial base characteristics as follow:(1)Horizontal and vertical expansion occured in the sphenoida region,(2)The anterial and posterior cranial bases circled S point opposite direction revolving,which caused cranial base angle to reduce significantly.2.The low-angle the cranial base characteristics was just opposite with high-angle′s.Conclusion:The shape of cranial base was different between high-angle and low-angle malocclusion patients.

OBJECTIVE To investigate the early events of norcantharidin (NCTD) induced cell apoptosis and cell cycle arrest, the variation of reactive oxygen species (ROS) and the NF-E2-relate? dactor 2/antioxidant response element(Nrf2/ARE) pathway in human HepG2 cells. METHODS The cyto?toxicity was measured by MTT assay. Apoptosis and cell cycle was analyzed by flow cytometry. The intra toxicity ROS production was evaluated by flow cytometry analysis with DCFH-DA probe and the effect of NCTD on Nrf2/ARE pathway was detected by luciferase assay in HepG2C8 cells under the same condition. The mRNA expression of heme oxygenase-1(HO-1) and NAD(P)H: quinone oxidoreductase 1(NQO1) antioxidase gene in Nrf2/ARE pathway downstream was evaluated by quantitative real-time PCR. RESULTS No significant cytotoxicity was detected after HepG2 cells were treated with NCTD 30, 60 and 120 μmol · L- 1 for 3 and 6 h, but cellular viability was inhibited significantly by NCTD 30, 60 and 120μmol·L-1 for 24, 48 and 72 h(P<0.01). Cell apoptosis and G2/M phase arrest occurred after HepG2 cells were treated with NCTD 60μmol · L-1 for 12, 24 and 48 h. The percentage of apoptosis increased from (4.00 ± 1.98)%to (12.10 ± 1.70)%for 12 h, from (4.05 ± 0.21)%to (31.8 ± 6.50)%for 24 h, and from (3.90 ± 0.85)% to (33.30 ± 1.41)% for 48 h, respectively. The percentage of G2/M phase increased from (16.51 ± 1.58)% to (40.89 ± 0.18)% for 12 h, from (16.99 ± 1.32)% to (55.29 ± 3.99)% for 24 h, and from (14.45 ± 0.59)% to (50.66 ± 5.88)% for 48 h, respectively. Compared with cell control group, the percentage of G1 phase had a significant decrease in the group with NCTD treated at different time points(P<0.01). No significant change in ROS in HepG2 cells was detected after the treatment with NCTD 30, 60 and 120μmol · L-1 for 3, 6 and 12 h. Nrf2/ARE pathway in HepG2C8 cells was activated by NCTD 30, 60 and 120μmol·L-1 for 6 and 12 h. mRNA expression of HO-1 and NQO1 had a signifi?cant activation in HepG2 cells after treatment with NCTD 30, 60 and 120 μmol · L-1 for 6 and 12 h (P<0.05). CONCLUSION NCTD can activate Nrf2/ARE pathway in the early stage in HepG2 cells, which may inhibit the intracellular ROS production in the early stage. Activation of ROS may not be the main event in NCTD induced HepG2 cell apoptosis and G2/M phase arrest.

Adult ; Humans ; Humeral Fractures ; diagnosis ; surgery ; Male ; Shoulder Dislocation ; diagnosis ; surgery

Functional dyspepsia (FD) is a common and frequently occurring disease in clinic. With the influence of environmental factors, social factors and dietary factors, the incidence rate of FD in the general population is yearly increasing. Traditional Chinese medicine has a long history and far-reaching influence in the treatment of FD. It can prevent and treat FD in the form of multiple-components, targets and channels, with obvious effect and prominent advantages. This article starts with the common syndrome types of FD, and discusses the research progress of single Chinese medicine, effective ingredients and the mechanism of traditional Chinese medicines in treating FD, in order to provide a theoretical basis for the treatment of FD with traditional Chinese medicines.

Objective To observe the effect of Gui Zhi Fu Ling Jiao Nang (GZFLJN) on the expressions of alpha smooth muscle actin (α-SMA) and proliferating cell nuclear antigen (PCNA) in uterine vascular smooth muscle cells (VSMC) of rat models with an intrauterine device (IUD) and to determine the thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) levels in peripheral blood. Methods Female Wistar rats were randomly divided into four groups:normal group (n=16,with normal breed without treatment),model group (n=18,drenching 0.9% normal saline after modeling of IUD),GZFLJN group (n=18),and aminocaproic acid tablets group (n=17). Immunohistochemical SP method was used to detect the expressions of α-SMA and PCNA in uterine VSMC.ELISA was served to detect the levels of TXB2 and 6-keto-PGF1α in peripheral blood. Results The positive rate of α-SMA were (50.89±9.41)%,(26.93±6.80)%,(48.92±6.80)%,and (34.63±7.26)%,respectively,in normal group,model group,GZFLJN group,and aminocaproic acid tablets group;obviously,it was significantly higher in normal group (t=14.43,P=0.00) and GZFLJN group (t=11.37,P=0.00) than that in model group and it was significantly lower in aminocaproic acid tablets group than in normal group (t=9.96,P=0.00) and GZFLJN group (t=8.23,P=0.00). The positive rate of PCNA were (25.66±7.24)%,(61.26±9.98)%,(28.36±9.17)%,and (50.23±8.71)%,respectively,in these four groups;obviously,it was significantly lower in the normal group (t=20.86,P=0.00) and GZFLJN group (t=19.12,P=0.00) than in model group and it was significantly higher in aminocaproic acid tablets group than in normal group (t=17.82,P=0.00) and GZFLJN group (t=16.05,P=0.00). Serum TXB2 level in these four groups were (445.86±24.43),(508.78±12.42),(448.11±9.63),and (498.11±13.63)ng/L;obviously,it was significantly higher in model group than in normal group (t=16.55,P=0.00) and aminocaproic acid tablets group (t=-4.12,P=0.00) and it was significantly lower in GZFLJN group than in model group (t=-15.23,P=0.00) and aminocaproic acid tablets group (t=-12.08,P=0.00). Serum 6-keto-PGF1α level in these four groups were (23.17±1.93),(18.09±0.93),(22.70±1.61),and (20.70±1.41)ng/L,respectively;obviously,it was significantly lower in model group than in normal group (t=-13.98,P=0.00) and aminocaproic acid tablets group (t=5.26,P=0.00) and it was significantly higher in GZFLJN group than in model group (t=11.43,P=0.00) and aminocaproic acid tablets group (t=8.76,P=0.00). Conclusion GZFLJN can regulate the expressions of α-SMA and PCNA of VSMC in the endometrium of IUD rats and the concentrations of TXB2 and 6-keto-PGF1α in the serum.

Objective Though paraquat (PQ) is highly toxic, there is still no effective treatment for PQ poisoning .The aim of the article was to study the protective effect and mechanism of the p 38 mitogen-activated protein kinase ( MAPK) inhibitor SB203580 on PQ-induced acute lung injury in rats . Methods 72 SD rats were randomly divided into three groups ( n=24 ): normal saline (NS) group, PQ poisoning group and p38 inhibitor SB203580 intervention (PQ+SB) group.The arterial blood gas analysis, lung wet and dry ratio (W/D),the expression of tumor necrosis factor-α(TNF-α), the superoxide dismutase (SOD) level and the pathological changes of lung tissues were recorded at different time points after drug intervention . Results On the 1st , 3rd, 5th days after drug intervention in PQ group, the alveolo-arterial oxygen partial pressure difference (PA-aO2) [(45.67 ±4.17), (68.78 ±6.63), (80.23 ±7.12 ) mmHg ], the lung tissue TNF-αexpression (14.63 ±3.10], [18.24 ±2.98], [16.22 ±2.79] pg/mg) and W/D ([4.931 ±0.034], [5.020 ±0.064], [5.079 ±0.016]) in-creased gradually to a peak on the 3rd day, while the SOD level de-creased respectively on the 1st , 3rd, 5th days after drug intervention ([175.26 ±7.98], [167.57 ±8.05], [160.24 ±6.78] U/ug) (P<0.05).Compared with PQ group, PQ+SB group got a decrease in the PA-aO2([80.23 ±7.12] vs [44.17 ±4.16]), the lung tissue TNF-αexpression ([16.22 ±2.79] vs [9.48 ±2.72]) and W/D ([4.805 ±0.070] vs [5.079 ±0.016]) (P<0.05), while the pulmonary SOD level increased in comparison with PQ group ([125.89 ±6.65] vs [160.24 ±6.78]) (P<0.05). Conclusion The p38MAPK inhibitor SB203580 plays a certain protective role in PQ-induced acute lung injury by reducing inflammation and improving antioxidant capacity .

Objective To investigate the relationships between the expression levels of tumor necrosis factor receptor associated factor 4 (TRAF4) and ribosomal S6 protein kinase 4 (RSK4) protein in gastric cancer tissues and the recurrence after laparoscopic radical gastrectomy. Methods In total, 176 patients were divided into the recurrence and non-recurrence group, and the expression levels of TRAF4 and RSK4 protein in cancer and adjacent tissues and in gastric cancer tissues in the recurrence and non-recurrence group were compared. The influencing factor of recurrence and the efficacy of TRAF4 and RSK4 protein expression in predicting recurrence were analyzed. Results The positive expression rate of TRAF4 protein in gastric cancer tissues was higher than that in adjacent tissues (P < 0.05) and that in the recurrence group was higher than that in the non-recurrence group (P < 0.05). The positive expression rate of RSK4 protein in gastric cancer tissues was lower than that in adjacent tissues (P < 0.05) and that in the recurrence group was lower than that in non-recurrence group (P < 0.05). The largest tumor diameter 5 cm, poor differentiation, TNM Ⅲ stage, depth of invasion T3-T4, lymph node metastasis, absence of adjuvant chemotherapy after operation, positive expression of TRAF4 and RSK4 protein, and regular diet w influenced the post-operative recurrence (all P < 0.05). The accuracy of TRAF4 and RSK4 protein in gastric cancer tissues in combined predicting the recurrence was 83.52%. Conclusion The expression of TRAF4 protein is high, and the RSK4 protein is low in gastric cancer tissue, which are related to recurrence.

Objective:To analyze the clinical characteristics and risk factors of impaired liver and renal function in hospitalized patients with gout.Methods:A total of 494 hospitalized patients with confirmed gout were selected and divided into four groups according to liver and renal function, control(Con), impaired liver function (ILF), impaired renal function (IRF), and both function impaired (ILRF) group. Multivariate logistic regression was used to analyze the risk factors related with impaired liver and renal function.Results:Compared to Con group, ILF group were younger with shorter gout duration, higher body mass index, waist circumference, homeostasis model assessment for insulin resistance (HOMA-IR), serum uric acid, low density lipoprotein-cholesterol (LDL-C), total cholesterol, triglycerides, C reactive protein, higher prevalence of dyslipidemia, obesity, fatty liver, and monosodium urate crystal (MSU) deposition (all P<0.05). IRF group were older and with higher serum uric acid, serum creatinine, C reactive protein, and hypertension, MSU deposition prevalence, with lower prevalence of fatty liver (all P<0.05). Compared to ILF group, IRF group were older, with longer gout duration, lower level of body mass index, waist circumference, HOMA-IR, LDL-C, total cholesterol, triglycerides, lower prevalence of obesity, fatty liver, and higher prevalence of hypertension and type 2 diabetes (all P<0.05). The univariate logistic regression analysis showed that age( OR=0.941, 95% CI 0.906-0.977, P<0.001), serum uric acid ( OR=1.002, 95% CI 1.000-1.005, P=0.043), HOMA-IR ( OR=1.147, 95% CI 1.024-1.285, P=0.018), and MSU deposition ( OR=1.959, 95% CI 1.154-3.326, P=0.013) were the independent risk factors of impaired liver function, while the independent risk factors of impaired renal function were age ( OR=1.104, 95% CI 1.048-1.162, P<0.001), serum uric acid ( OR=1.007, 95% CI 1.004-1.010, P<0.001), and MSU deposition ( OR=2.393, 95% CI 1.191-4.805, P=0.014). Conclusions:Serum uric acid and MSU deposition are the common independent risk factors for impaired liver and renal function in patients with gout. Younger patients with insulin resistance are susceptible to impaired liver function, older patients with hypertension and diabetes are susceptible to impaired renal function.

Objective:To investigate the effects of mobile intelligent cognitive training(MICT)on cognitive function,ac-tivities of daily living,and quality of life of patients with mild to moderate cognitive impairment after stroke in the community. Method:Ninty-two patients with mild to moderate cognitive impairment after stroke in the community were randomly divided into experimental group(n=46)and control group(n=46).Both groups received comprehen-sive management,and the experimental group received additional MICT for 12 weeks.The Mini-Mental State Examination(MMSE),Montreal Cognitive Assessment(MoCA),Prospective and Retrospective Memory Ques-tionnaire(PRMQ),Modified Barthel Index(MBI),Functional Activities Questionnaire(FAQ),and Quality of Life Index(QLI)were assessed before,4 weeks and 8 weeks after treatment,and 1-month follow-up after the end of treatment. Result:Before treatment,there was no significant difference in all scores between the two groups(P＞0.05).After 6 weeks of treatment,the MMSE,PRMQ,MBI,FAQ,and QLI scores of the two groups were im-proved(P＜0.05),and the MoCA,MBI,and QLI scores of the experimental group were higher than those of the control group(P＜0.05);After 12 weeks of treatment and 1 month of follow-up,the scores of MMSE,MoCA,PRMQ,MBI,FAQ,and QLI were significantly improved in two groups(P＜0.05),and all scores of the experimental group were better than those of the control group(P＜0.05). Conclusion:MICT can improve cognitive function,activities of daily living,and quality of life in patients with mild to moderate cognitive impairment after stroke in the community,with efficacy lasting at least 1 month.

Objective:To disclose the genome characteristics and mutations of 2019 novel coronavirus (2019-nCoV) strains from the imported cases of Coronavirus Disease 2019 (COVID-19) in Gansu province, thereby to provide scientific reference for the prevention and control of COVID-19 in Gansu province. Methods:The respiratory tract specimens of imported COVID-19 cases from seven countries in Gansu province in 2020 were collected. The virus genome was sequenced by the second-generation sequencing technology, the whole genome sequences were compared and analyzed, and the MEGA software was used to construct a phylogenetic tree based on the neighbor-joining method.Results:A total of 46 2019-nCoV genome sequences with a length of 29 605~29 903 bp were obtained. Compared with the Wuhan reference strain (GenBank ID: NC_045512.2), it was found that the median (minimum to maximum) number of the nucleotide mutations of the 2019-nCoV genome sequence of the imported cases was 10 (7-24). A total of 134 nucleotide mutation sites were found in all 2019-nCoV genome sequences from 7 entry countries in Gansu province, distributed in 11 open reading frames (ORFs). The top three nucleotide mutations in different proteins: ORF1ab (78), S(20), N(12). Among the 134 nucleotide mutations, 82 caused amino acid mutations, and all of them were missense mutations. No insertions or deletions were seen. Types of deletion mutations, the top three amino acid mutations in different proteins: ORF1ab (46), S(11), N(10); the key sites of the receptor binding domain (RBD) of the S protein have not been mutated.Conclusions:No imported cases in Gansu province have been found to carry the reported mutations that can clearly lead to changes in the spread and pathogenicity of 2019-nCoV.