Objective To investigate the associations between the single nucleotide polymorphisms of TERT rs2736098, CLK3 rs11543198 and bladder cancer. Methods 201 bladder cancer cases and 200 healthy controls were included in the research, and the genotypes of TERT rs2736098 and CLK3 rs11543198 were determined using the PCR-RFLP method. Relationship between genotypes and bladder cancer risks was investigated. Results There were statistical significance in the rs2736098 genotype frequencies and allele frequencies between cases and controls (χ2= 6.973, P = 0.031; χ2= 7.412, P = 0.006). Compared with the individuals with the GG genotype , the risk of bladder cancer increased 2.069 times with the AA genotype (OR = 2.069, 95%CI: 1.181-3.624, P = 0.011). And there were no significant associations between the rs11543198 genotype frequencies and allele frequencies between cases and controls (χ2 = 0.202, P = 0.904; χ2 = 0.188, P = 0.665). Rs2736098 and rs11543198 genotype distribution in bladder cancer pathologic grade and stage had no statistical significance (P > 0.05). Conclusion Rs2736098 polymorphism is associated with risk of bladder cancer and rs11543198 polymorphism was not associated with risk of bladder cancer.

Objective To analyze the expression level of kinesin family member 26B(KIF26B)in bladder cancer based on the pub-lic database,and to investigate the effect of silencing KIF26B on the proliferation,invasion and migration of bladder cancer cells.Methods The expression of KIF26B in bladder cancer and its relationship with survival and prognosis were analyzed based on GEO and UaLcan databases.Real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to detect the expression of KIF26B in bladder cancer cell lines(T24,J82)and normal bladder epithelial cells SV-HUV-1.si-KIF26B and si-NC fragments were transfected into T24 cells,and the effects of silencing KIF26B on the proliferation,invasion and migration of T24 cells were detected by methyl thiazolyl tetrazolium(MTT)assay,Transwell assay and scratch assay.Western blot was used to detect the expression levels of p-MEK,MEK,ERK and p-ERK proteins after the silencing of KIF26B.Results KIF26B was highly expressed in bladder cancer tis-sues,and the prognosis of patients with high expression of KIF26B was worse(P＜0.05).The expression level of KIF26B in bladder cancer cell lines T24 and J82 was significantly higher than that in normal bladder epithelial cells SV-HUV-1(P＜0.05).After the si-lencing of KIF26B gene,MTT,Transwell and scratch assay Results showed that the proliferation,invasion and migration ability of T24 cells were significantly decreased(P＜0.05);After silencing KIF26B,the expression of p-MEK and p-ERK proteins in T24 cells was down-regulated(P＜0.05),while MEK and ERK proteins had no significant changes(P＞0.05).Conclusion KIF26B is highly ex-pressed in bladder cancer tissues and cells,which is associated with poor prognosis of patients.Silencing KIF26B can inhibit the prolifera-tion,invasion and migration of bladder cancer cells,and the mechanism may play a role through the MEK/ERK pathway.

Objective To investigate the efficacy of negative pressure combined ureteroscopy combined with flexible ureteroscopy holmium laser lithotripsy in the treatment of renal calculi or upper ureteral calculi with a maximum diameter of 2-4cm.Methods The data of patients with renal calculi or upper ureteral calculi with a maximum diameter of 2-4cm who were hospitalized inAffiliated Hospital of Youjiang Medical University for Nationalities from March 2023 to March 2024 were collected and divided into observation group(n=40)and control group(n=40).The observation group was treated with negative pressure combined ureteroscopy combined with flexible ureteroscopy for holmium laser lithotripsy,and control group was treated with nephroscope for percutaneous nephrolithotomy(PCNL).The operation time,postoperative hospital stay,changes of blood routine white blood cell(WBC)before and after operation,changes of urine routine WBC before and after operation,postoperative visual analogue score(VAS),one-stage stone clearance rate and postoperative complications were compared between two groups.Results The postoperative hospitalization time and VAS of observation group were significantly lower than those of control group(P＜0.05).There was no significant difference in operation time,stone clearance rate,postoperative blood routine WBC and postoperative urine routine WBC between two groups(P＞0.05).However,the blood routine WBC of two groups after operation was higher than that before operation(P＜0.05).The overall stone clearance rate of two surgical methods was 86.25％,of which the stone clearance rate of observation group was 82.5％,and control group was 90％.There was no significant difference between two groups(P＞0.05).Conclusion It is safe and effective to treat renal calculi;or upper ureteral calculi with a maximum diameter of 2-4cm by negative pressure combined ureteroscopy combined with flexible ureteroscopy holmium laser lithotripsy.It is expected to partially replace PCNL and become another safe and reliable operation for the treatment of renal calculi or upper ureteral calculi with a maximum diameter of 2-4cm.