1.Evaluation of Measurement Uncertainty of 2 2 Clinical Chemistry Assays
Journal of Modern Laboratory Medicine 2014;(6):156-158
Objective To calculate the measurement uncertainty of clinical chemical analytes according to the internal quality control (IQC)and external quality assessment (EQA)data in clinical laboratory.Methods Collected the IQC data from January to June 2013 and EQA data between 2012~2014 of clinical chemistry in clinical laboratory.Calculated the measure-ment uncertainty and extended uncertainty according to the Nordtest criteria.Results It was effective to evaluate the uncer-tainty using IQC and EQA data.ALP ranked the highest extended uncertainty and Na+ ranked the lowest uncertainty.The range of uncertainty varies greatly,electrolyte 4.27~18.16,enzyme 8.12~24.88,small molecular 4.88~12.44,protein and lipids 4.78~13.1.Conclusion The evaluation of clinical chemistry uncertainty by IQC and EQA data is simple and practi-cal,which is beneficial for assurancing the measurement accuracy.
2.Asymmetric index analysis on the orthodontic-orthognathic treatment of facial asymmetry patients in skull positioning posterior-anterior radiographs.
West China Journal of Stomatology 2014;32(2):138-144
OBJECTIVETo analyze the asymmetry of measurements in facial asymmetry patients with skull positioning posterior-anterior radiographs after orthodontic-orthognathic treatment.
METHODSPosterior-anterior cephalometric radiography and cephalometric analysis were performed in forty-five patients with different degrees of facial asymmetry. A single sample t-test was conducted to compare the asymmetry of measurements before treatment and individual normal occlusion. The measurement and reference values in facial asymmetry patients who underwent orthodontic-orthognathic treatment were summated by analyzing the correlation coefficient. The paired t-test was employed to compare the difference between the two groups before and after the treatment.
RESULTSFourteen measurements were significantly different before the treatment and individual normal occlusion (P < 0.05). Me[X] exhibited a higher correlation index of Ag[X] in forty-five cases. Twenty-eight patients showed the main asymmetry in the mandibular body, whereas fifteen patients in the ramus. Two patients showed the main asymmetry in both mandibular body and ramus. Fifteen measurements were significantly different before and after the treatment (P < 0.05).
CONCLUSIONFacial asymmetry is mainly concentrated in one-third of the surface, primarily manifested in the mandibular body. Orthodontic-orthognathic treatment is preferred when Ag[X] is greater than the minimum reference value of 11.31%, Go[X] is greater than 9.79%, and Me[X] is greater than 5.2 mm.
Cephalometry ; Face ; abnormalities ; Facial Asymmetry ; congenital ; Humans ; Hyperplasia ; Mandible ; Skull
3.Promote multidisciplinary study on anthracycline-induced cardiotoxicity in children.
Shu-bao CHEN ; Mei-rong HUANG ; Jing-yan TANG
Chinese Journal of Pediatrics 2013;51(8):565-568
Anthracyclines
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administration & dosage
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adverse effects
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Antineoplastic Agents
;
administration & dosage
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adverse effects
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Biomarkers
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blood
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Cardiomyopathies
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chemically induced
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diagnosis
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prevention & control
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Cardiotonic Agents
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therapeutic use
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Child
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Child, Preschool
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Echocardiography
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Heart
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drug effects
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Heart Diseases
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chemically induced
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diagnosis
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prevention & control
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Humans
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Risk Factors
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Survival Analysis
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Troponin I
;
analysis
6.Experimental Studies on Acute Oral Toxicity, Skin Irritation and Skin Allergy of Thuja Essential Oil
Jin PENG ; Lu TANG ; Yan XIAO ; Yang CHEN ; Meihua BAO
China Pharmacist 2016;19(7):1420-1422
Objective:To evaluate the acute oral toxicity , skin irritation and skin allergy of Thuja essential oil ( TEO) , and pro-vide experimental basis for the clinical use of TEO .Methods:The acute oral toxicity was measured by Horn ’ s assay .Totally 40 KM mice were divided into four groups and intragastrically administered with TEO at different dose of 21.50, 10.00, 4.64 and 2.15 g · kg-1 .After the 14-day observation, the death number and toxic manifestations were recorded and observed , and LD50 was calculated by checking the Horn's form of LD50 .The skin irritation test was performed on healthy adult white rabbits .Totally 9 rabbits were divid-ed into 3 groups randomly , and TEO at the concentration of 100%, 50%and 25%was painted on the skin of the rabbits .Edible vege-table oil was used as the negative control .The erythema and edema of the treated skin were evaluated and scored .Delayed skin hyper-sensitivity reaction was used to investigate the allergy of TEO .Totally 30 white guinea pigs were randomly divided into 3 groups:TEO group, the negative control (edible vegetable oil) and the positive group (1%2, 4-dinitrochlorobenzene).After the intracutaneous in-duction stage and local induction stage , TEO was used to activate the hypersensitive reaction .The skin response was observed and scored after the 24-hour and 48-hour activation.Results:The mice in 21.50 g · kg-1 TEO treatment group were all dead , while only a part of the mice in 10.00 and 4.64 g · kg-1 TEO treatment groups were dead , and no mice died in 2.15 g · kg-1 TEO treatment group.According to the Horn's form of LD50 , LD50 of TEO was 9.26 g · kg -1 for male mice and 7.94 g · kg -1 for female mice.The results of skin irritation test indicated the strong irritation effects of TEO .However , the irritation of TEO was reduced after the dilution , and 25%TEO showed no irritation to the skin of rabbits .The results of delayed skin hypersensitivity reaction showed obvious erythema and edema induced by 2, 4-dinitrochlorobenzene , while no obvious erythema and edema were found in TEO treated guinea pigs , indi-cating non-allergic effect of TEO .Conclusion:TEO has strong skin irritation in rabbits , while no obvious oral toxicity in mice and skin allergy in guinea pigs .
7.Evaluation of the early outcomes of mini-open lumbar microdiscectomy
Xin-Sheng PENG ; Li-Yan CHEN ; Fo-Bao LI ;
Chinese Journal of Microsurgery 2006;0(06):-
Objective To evaluate the early outcomes of mini-open lumbar microdiscectomy. Methods There were 38 cases in each group of mini-open lumbar mierodiscectomy and conventional discecto- my.Operating time,blood loss,time of leaving the bed and length of hospital stay were compared in two groups.MacNab criteria were used to evaluate the outcomes.Results To compare the conventional discec- tomy group,microdiscectomy group spent similar operating time,but had less blood loss(P
8.The analysis and identification of chrysanthemums from the different producing areas by IR spectroscopy
Yan BAI ; Hongjuan BAO ; Dong WANG ; Zhihong CHEN ; Yuxia LOU
Chinese Traditional Patent Medicine 1992;0(12):-
AIM: To get the IR spectrums of chrysanthemums from the different producing areas,and to find out the characters of IR spectrums,and the data of IR spectroscopy of chrysanthemums from the different producing areas. METHODS: Using Fourier transform infrared spectroscopy. RESULTS: The FTIR spectrums、Second-order derivative spectrums、Two-dimensional spectrums of chrysanthemums from the different producing areas have their obvious IR characters. CONCLUSION: We can discriminate chrysanthemums from the different producing areas macroscopically and holistically by IR spectroscopy.IR spectroscopy can give us the digital descriptions of TCM,so it is a new analytical method to discriminate TCM.
9.Analysis on the different extracted parts of Flos Chrysanthemi from different regions by FT-IR
Yan BAI ; Hongjuan BAO ; Zhihong CHEN ; Dong WANG
Chinese Traditional Patent Medicine 1992;0(03):-
AIM: To get the IR spectrums of the different extracted parts of chrysanthemum, and to find out the characters of IR spectrums. METHODS: To use Fourier transform infrared spectroscopy. RESULTS: The FTIR spectrums of the different extracted parts of chrysanthemum had their obvious IR characters. CONCLUSION: IR spectroscopy can give us the digital descriptions of TCM, so it is a new and scientific analytical method to discriminate TCM.
10.Several Sterilizing Methods for Paraffin Oil:A Comparative Experiment
Yan REN ; Guohua CHEN ; Jianfang BAO ; Dongping HE
Chinese Journal of Nosocomiology 1994;0(01):-
OBJECTIVE To study effective and convenient method for paraffin oil sterilization.METHODS By using carrier qualitative germicidal test,to compare pressure steam sterilization,dry heat sterilization and cobalt-60(gamma)-ray radiation sterilization to test the sterilizing effect and operating procedure.RESULTS Pressure steam sterilization was unable to achieve 100% sterilizing effect,whether we extended the time or use the intermittent(sterilization).After dry heat or radiation sterilization processes,no microorganism was found.CONCLUSIONS Effect of sterilization with dry heat or radiation sterilization is trustable,but its packing,operation and equipment are requested strictly,and pressure steam sterilization may be not good for paraffin oil.