1.Characteristic of urinary protein spectrum in patients with stage III diabetic nephropathy and its regression analysis with traditional Chinese medicine symptom.
Yi-Gang WAN ; Xian-Jie MENG ; Shan-Mei SHEN ; Xun-Yang LUO ; Liu-Bao GU ; Xi-Miao SHI ; Jian YAO
China Journal of Chinese Materia Medica 2013;38(23):4157-4163
To analyze the characteristic of urinary protein spectrum in patients with stage III diabetic nephropathy (DN) and its compliance with traditional Chinese medicine (TCM)symptom, for the sake of providing a basis for clarifying the rules of TCM syndrome differentiation in DN. Adopting the traditional epidemiological retrospective method, thirty-eight TCM syndromes and urinary protein with medium or low molecular weight, as well as urinary enzyme, including 24 h urinary protein (Upro), urinary albumin( UAlb), urinary retinal binding protein( URBP), urinary cystatin C (UCysC), urinary N-acetyl-beta-D-glucosaminidase (UNAG), were collected from 108 patients with stage III DN, and a multiple factor regression analysis between them was conducted. As the results, the levels of Upro, UAlb, URBP, UCysC, and UNAG were increased in 108 patients with stage III DN. Qi-Yin deficiency type was the major type. The level of UAlb in patients with Qi-Yin deficiency type was significantly higher than those without Qi-Yin deficiency type (P < 0.05). The elevation of Upro with the factors as swift digestion with rapid hungering, lassitude and lack of strength, weakness of waist and knees was complied, the elevation of UA1b with the factors as dry mouth with desire to drink, the elevation of URBP with the factors as numbness of extremities, shortness of breath, the elevation of UCysC with the factors as clear urine in large amounts, and the elevation of UNAG with the factors as frequent micturition, were complied respectively. In conclusion, for 108 stage III DN patients. The increase in urinary protein spectrum including UAlb, URBP, UCysC, and UNAG is the major characteristic. Shen and Pi are the major organs related to the appearance of urinary protein; Pi-Shen deficiency is the basic pathogenesis. The level of UAlb is taken as one of the objective syndrome factors for Qi-Yin deficiency type. The levels of UNAG and UCysC are possibly the objective syndrome factors for Shen-Qi deficiency type.
Diabetic Nephropathies
;
complications
;
diagnosis
;
urine
;
Female
;
Humans
;
Male
;
Medicine, Chinese Traditional
;
methods
;
Middle Aged
;
Proteinuria
;
complications
;
urine
;
Qi
;
Regression Analysis
;
Yin-Yang
2.Analysis on disease burden of occupational coal workers′ pneumoconiosis in Anhui Province
HU Qiong CHEN Bao chun HU Xun jia XIE Qiao mei ZHA Wan sheng SONG Yuan chao
China Occupational Medicine 2022;49(06):635-639
Objective
To analyze the disease burden caused by occupational coal workers' pneumoconiosis (CWP) in Anhui
Methods
Province. A total of 1 649 patients with occupational CWP diagnosed in Anhui Province from 1950 to 2019 were
selected as the study subjects using a retrospective study method. Age, gender, survival time, location, working age of disease
onset, age of death, stage and date of diagnosis of CWP, comorbidities at the time of investigation, hospitalization and outpatient
expenses in the past year, cost of absence from work, cost of absence from work of caregivers, and cost of nutrition and
transportation were investigated. The years of life lost, years lived with disability (YLDs), disability adjusted life years (DALYs)
Results
and economic losses were calculated. Among these patients, 1 405 cases survived and 244 cases died. In the age range
- - -
of 20.0 70.0 years, the YLDs of surviving patients were 2.12 22.20 (8.19±3.59) person years. The YLDs of patients with disease
vs - P
complications was higher than that of patients without complications [(8.55±3.95) (7.87±3.21) person years, <0.01]. The total
- -
DALYs of the patients was 14 031.59 person years, and the average per capita DALYs was 8.51 person years. Its YLDs
accounted for 82.0 % of the total DALYs. The total economic loss caused by CWP in the 1 405 surviving patients was 354.903 0
Conclusion
million yuan, and the average per capita economic loss was 252 600 yuan. The disease burden caused by CWP is
relatively high in Anhui Province. In addition to early detection, diagnosis and treatment, it is necessary to focus on prevention
and treatment of CWP complications to reduce the disease burden.
3.Efficacy of anti-CD19 CAR-T cell therapy in 10 refractory recurrent B cell malignancies.
Fang BAO ; Kai HU ; Wei WAN ; Lei TIAN ; Hong Mei JING
Chinese Journal of Hematology 2018;39(6):454-459
Objective: To investigate the outcomes of anti-CD19 CAR-T cell for relapsed and refractory B cell malignancies. Method: Ten patients with relapsed and refractory B cell acute lymphocytic leukemia (B-ALL) and non-Hodgkin's lymphoma (NHL), diagnosed in the Department of Hematology of Peking University third Hospital from December 2015 to July 2017, were treated with anti-CD19 CAR-T cell therapy, and the efficacy and safety were analyzed. Results: Efficacy was assessed on the 28th day after cell infusion, including 66.7% (4/6) of complete remission (CR) for patients with ALL, 16.7% (1/6) of partial remission (PR), and 83.3% (5/6) of overall response rate (ORR). For NHL patients, CR was 33.3% (1/3) and most of the lesions disappeared in a patient with mantle cell lymphoma, but residual lesion presented persistent state. After infusion of anti-CD19 CAR-T cells, the main side effect was cytokine release syndrome (CRS) and fever. One patient presented with aphasia and the other one had multiple organ failure, which were improved after treatment. No patients died of CRS. Conclusion: anti-CD19 CAR-T cell for relapsed and refractory B cells hematological malignancies is safe, and the most problematic side effect is CRS, which can be controlled by therapy.
Antigens, CD19
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B-Lymphocytes
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Cell- and Tissue-Based Therapy
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Humans
;
Leukemia, B-Cell
;
Receptors, Antigen, T-Cell
;
Recurrence
;
T-Lymphocytes
4.Clinical implication of urinary protein markers in diabetic nephropathy and interventional effects of Chinese herbal medicine.
Xi-Miao SHI ; Xian-Jie MENG ; Yi-Gang WAN ; Shan-Mei SHEN ; Xun-Yang LUO ; Liu-Bao GU ; Jian YAO
China Journal of Chinese Materia Medica 2014;39(14):2589-2594
In clinic, some urinary protein makers can dynamically and noninvasively reflect the degree of renal tubular injury in patients with diabetic nephropathy (DN). These urinary biomarkers of tubular damage are broadly divided into two categories. One is newfound, including kidney injury molecule-1 (Kim-1), neutrophil getatinase-associated lipocalin (NGAL), liver-type fatty acid-binding protein (L-FABP) and cystatin C (CysC); the other one is classical, including beta2 microglobulin (beta2-MG), retinal binding protein (RBP) and N-acetyl-beta-D-glucosaminidase (NAG). It is reported that, the increases in urinary protein markers are not only closely related to the damage of tubular epithelial cells in DN patients, but also can be ameliorated by the treatment with Chinese herbal compound preparations or Chinese herbal medicine. Recently, although urinary proteomics are used in the protein separation and identification, the traditional associated detection of urinary protein markers is more practical in clinic. At present, it is possible that the associated detection of urinary biomarkers of glomerular and tubular damages may be a feasible measure to reveal the clinical significance of urinary protein markers in DN patients and the interventional effects of Chinese herbal medicine.
Biomarkers
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urine
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Diabetic Nephropathies
;
complications
;
drug therapy
;
urine
;
Drugs, Chinese Herbal
;
pharmacology
;
therapeutic use
;
Humans
;
Medicine, Chinese Traditional
;
methods
;
Proteinuria
;
complications
5.Case-control study on two suturing methods for the repairing of complete rupture of the deltoid ligament.
Tao ZHANG ; Chun-you WAN ; Bao-tong MA ; Wei-guo XU ; Xiao-long MEI ; Peng JIA ; Lei LIU
China Journal of Orthopaedics and Traumatology 2016;29(5):408-414
OBJECTIVETo compare clinical outcomes between two suturing methods using non absorbable materials through drilling the bone and suturing anchors for the treatment of complete rupture of the deltoid ligament.
METHODSFrom January 2009 to January 2013, 58 hospitalized patients with ankle fracture combined with complete rupture of the deltoid ligament were treated with suturing using non absorbable materials through drilling the bone or suturing anchors. There were 29 patients who received suturing treatments using non absorbable materials through drilling the bone (Group A), including 18 males and 11 females, with an average age of (39.76 +/- 11.81) years old. According to the Lauge-Hansen classification, 12 patients had supination external rotation (SER) injuries with IV degree, 5 patients had pronation external rotation (PER) injuries with III degree, 10 patients had PER injuries with IV degrss, and 2 patients had pronation abduction injuries with III degree. There were 29 patients who received treatments with suturing using anchors (Group B), including 14 males and 15 females, with an average age of (41.79 +/- 13.28) years old. According to the Lauge-Hansen classification,9 patients had SER injuries with IV degree, 6 patients had PER injuries with III degree,13 patients had PER injuries with IV degree, and 1 patient had pronation abduction injuries with III degree. All the patients were treated with open reduction and internal fixation, as well as reconstruction of deltoid ligaments to restore the stability of the medial ankle structures. The clinical examination, imaging evaluation, American society for ankle surgery (AOFAS) ankle-hindfoot score and visual analogue scale (VAS) were used to evaluate the clinical results after operation, and the results of the two groups were compared and analyzed statistically.
RESULTSThe follow-up duration of the 58 patients ranged from 23 to 40 months,with an average of 27.3 months. All the patients had fracture union, and the mean healing time was 12.3 weeks (ranged, 10 to 17 weeks). There were no incision complications and ankle instability. There were no significant differences between two groups in AOFAS (P=0.666) and the VAS (P=0.905).
CONCLUSIONTreatments of complete rupture of the deltiod ligaments with the two suturing methods get similar good clinical effects, but the suturing using non absorbable materials through drilling the bone has several advantages such as reducing the financial burden of patients, saving social medical resources and avoiding the shortcoming in difficult removal of anchor suture.
Adolescent ; Adult ; Ankle Fractures ; surgery ; Ankle Injuries ; surgery ; Ankle Joint ; surgery ; Case-Control Studies ; Female ; Fracture Fixation, Internal ; Humans ; Lateral Ligament, Ankle ; injuries ; surgery ; Male ; Middle Aged ; Young Adult
6.Cleavage of HCV by HCV specific deoxyribozyme in vitro.
Xiao-yu WEN ; Wan-guo BAO ; Xiu-yun YANG ; Mei-mei TIAN ; Feng WANG ; Jun-qi NIU
Chinese Journal of Hepatology 2005;13(12):900-902
OBJECTIVETo study the cleavage activity of specific deoxyribozyme to hepatitis C virus in vitro.
METHODSThree deoxyribozymes were designed to cleave at sites 157, 168, 173 in HCV 5'-noncoding region with the active region of 5'-GGCTAGCTACAACGA-3' respectively. Plasmid pCMV/T7-NCRC -Delta Luc was completely linearized with restriction endonuclease Xba I. HCV RNA5'-NCRC was transcribed in vitro from the linearized products and radiolabelled with [alpha-32P] UTP. Under the conditions of 37 degrees C, pH7.5, Mg2+ 10 mmol/L, the three deoxyribozymes were mixed with substrate RNA individually for 120 minutes and then the reactions were terminated. The cleavaged products were separated with 8% denaturated polyacrylamide gel electrophoresis and displayed by autoradiography. DRz3 was mixed with the substrate RNA at different Mg2+ concentrations. The cleavage efficiency was analyzed with a gel document action analyzing systems.
RESULTSUnder the adopted conditions the three deoxyribozymes efficiently cleaved to the target RNA in vitro and the cleavage activity of DRz3 was increased with the increase of Mg2+ concentration.
CONCLUSIONThe designed deoxyribozymes can cleave 5'-NCR mRNA of HCV efficiently in vitro and it is dose-respondent to Mg2+ concentration.
DNA, Catalytic ; genetics ; DNA, Single-Stranded ; genetics ; Genetic Therapy ; Hepacivirus ; genetics ; Hepatitis C ; therapy ; Humans ; RNA, Messenger ; genetics
7.Effect of interferon neutralization antibody in the treatment of chronic hepatitis B with interferon.
Xian-rui CHEN ; Hui-liang WANG ; Mei-xiang XUAN ; Xin-gang MAO ; Jian-guo SUN ; Bao-mei WAN
Chinese Journal of Hepatology 2009;17(5):385-386
Adult
;
Antibodies
;
blood
;
Antiviral Agents
;
therapeutic use
;
DNA, Viral
;
blood
;
Female
;
Hepatitis B Surface Antigens
;
blood
;
Hepatitis B e Antigens
;
blood
;
Hepatitis B virus
;
drug effects
;
Hepatitis B, Chronic
;
blood
;
drug therapy
;
virology
;
Humans
;
Interferons
;
immunology
;
therapeutic use
;
Liver Function Tests
;
Male
;
Middle Aged
;
Young Adult
8.Implication of liver function in hemorrhagic fever patients with renal syndrome.
Bao-mei WAN ; He-lou LI ; Jing WANG ; Hui-liang WANG ; Qiang GUO
Chinese Journal of Hepatology 2005;13(5):387-388
Adolescent
;
Adult
;
Aged
;
Child
;
Female
;
Hemorrhagic Fever with Renal Syndrome
;
physiopathology
;
Humans
;
Liver
;
physiopathology
;
Liver Function Tests
;
Male
;
Middle Aged
;
Prognosis
10.Genistein Improves the Major Depression through Suppressing the Expression of miR-221/222 by Targeting Connexin 43.
Fang SHEN ; Wan li HUANG ; Bao ping XING ; Xiang FANG ; Mei FENG ; Chun ming JIANG
Psychiatry Investigation 2018;15(10):919-925
OBJECTIVE: Recent studies have indicated the possibility that genistein may improve depression via regulating the expression of miR-221/222. This study is to explore whether genistein could improve depression by altering miR-221/222 levels and investigate the possible mechanisms involved in the improvement effect of genistein. METHODS: The animal model of depression was established through unpredictable chronic mild stress. Nest building test and splash test were adapted to evaluate the effects of genistein on depressive symptoms in mice. qRT-PCR and western blot analysis were used to detect the expression of miR-221/222 and connexin 43 (Cx43) in the prefrontal cortex of the mice. In vitro, U87-MG astrocytes were treated with genistein and the expression of miR-221/222 and Cx43 was measured. The dual-luciferase reporter assay was used to verify whether Cx43 was a direct target of miR-221/222. RESULTS: The behavioral tests showed that genistein could significantly reduce depression symptoms of mice, and this remission was not affected by gender. Genistein in vivo and in vitro could reduce increased levels of miR-221 and miR-222 in the prefrontal cortex of depressed mice, while upregulate Cx43 expression. Dual-luciferase reporter assay suggested Cx43 was directly regulated by miR-221/222 in astrocytes. CONCLUSION: Genistein can play its antidepressant effect through down-regulating miR-221/222 by targeting Cx43.
Animals
;
Astrocytes
;
Behavior Rating Scale
;
Blotting, Western
;
Connexin 43*
;
Depression*
;
Genistein*
;
In Vitro Techniques
;
Mice
;
Models, Animal
;
Prefrontal Cortex