4.A comparison of the pharmacological actions of seven constituents isolated from fructus schizadrae (author's transl).
Bao TIAN-TONG ; Tu GUI-FANG ; Liu GENG-TAO ; Sun RUN-HUA ; Song ZHEN-YU
Acta Pharmaceutica Sinica 1979;14(1):1-7
Alanine Transaminase
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metabolism
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Animals
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Liver
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drug effects
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Male
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Mice
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Plant Extracts
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pharmacology
5.In vitro differentiaion of peripheral blood mononuclear cells into smooth muscle progenitor cells
wen-yi, YANG ; yi-wen, YAN ; jiang, HONG ; bao-gui, SUN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(03):-
Objective To optimize methods of culturing smooth muscle progenitor cells(SPCs) from mononuclear cells(MNCs) of peripheral blood. Methods Human MNCs isolated from buffy coat were seeded on M-199 with bovine pituitary extraction.On the eighth day outgrowth cells were stimulated with platelet-derived growth factor-BB(PDGF-BB).Fifteen days later,immunofluorescence,Western blot or RT-PCR was used to analyzed the expression of smooth muscle cell specific ?-actin(?-SMA),smooth muscle myosin heavy chain(SM MHC),Calponin,CD34,Tie-2 and Flk-1,and fluorescence activated cell sorter was employed to examine ?-SMA positive cells ratio. Results The cells stimulated by PDGF-BB for 15 d were positive for ?-SMA,SM MHC,Calponin,CD34 and Flk-1,but negative for Tie-2.The ?-SMA positive cells ratio was(90.57?5.63)%,significantly different from that of the control(P
6.Blood pressure changes post liver transplantation in 206 recipients.
Hai LIU ; Qiu-yan DAI ; Zhi-hai PENG ; Bao-gui SUN
Chinese Journal of Cardiology 2006;34(10):902-904
OBJECTIVETo study the blood pressure (BP) changes in the liver transplant recipients.
METHODSA total of 206 patients without preoperation hypertension received liver transplantation in our hospital from February 2001 to July 2005. The BP level and serum immunosuppressant concentration at preoperation and various time points post operation were determined.
RESULTSCompared with the preoperation, the average systolic and diastolic pressure was significantly increased at the 2 week, 1, 2, 4 and 6 months post operation. The mobility of hypertension increased significantly after liver transplantation, with the highest mobility (46.49%) at the 1st month post operation. There was no linear correlation between the immunosuppressant (FK506) concentration and the BP level at any time point.
CONCLUSIONThere was a high hypertension incidence after liver transplantation. Although the use of immunosuppressive drugs accompanied with the BP increase, there was no linear correlation between the immunosuppressant concentration and the BP level post operation.
Blood Pressure ; Female ; Follow-Up Studies ; Humans ; Hypertension ; etiology ; Immunosuppressive Agents ; adverse effects ; Liver Transplantation ; adverse effects ; Male ; Middle Aged ; Postoperative Complications ; physiopathology ; Retrospective Studies
7.Expression of anti-gp96 scFv fragment in Pichia pastoris and identification of its biological activity.
Mingming GUI ; Huiying WU ; Lu SUN ; Yaxing XU ; Bao ZHAO ; Xin LI ; Changfei LI ; Xidong WANG ; Songdong MENG
Chinese Journal of Biotechnology 2014;30(4):595-604
Secretory anti-gp96 scFv fragment was expressed in Pichia pastoris to obtain a small molecule antibody that specifically recognizes heat shock protein gp96. The gp96-scFv fragment gene was synthesized and cloned to Pichia pastoris expression plasmid pPICZa-A. Pichia pastoris X33 was electroporated with the linearized recombinant expression vector, and expression of gp96-scFv fragment was induced by methanol. The His-tagged recombinant protein was then purified by affinity chromatography and analyzed with SDS-PAGE and Western blotting assays. The biological activities of recombinant gp96-scFv fragment were determined by Western blotting, Immunofluorescence, ELISA and FACS assays. The gp96-scFv fragment was expressed successfully in Pichia pastoris. About 50 mg of recombinant protein could be purified from 1 liter of the Pichia pastoris culture supernatant. Its molecular weight was about 15 kDa. The gp96-scFv fragment could specifically bind to gp96 protein by Western blotting, immunofluorescence, ELISA and FACS analyses. Pichia pastoris-expressed gp96-scFv fragment specifically recognizes gp96 protein, which could be used for Western blotting, Immunofluorescence, ELISA and FACS analyses.
Blotting, Western
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Chromatography, Affinity
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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Membrane Glycoproteins
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immunology
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Pichia
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metabolism
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Plasmids
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Recombinant Proteins
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biosynthesis
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Single-Chain Antibodies
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biosynthesis
8.Safety and efficacy of cryothermal and radiofrequency catheter ablation in treatment of typical atrial flutter.
Fang WANG ; Cong-xin HUANG ; Gang CHEN ; Feng ZHANG ; Wei-dong MENG ; Bao-gui SUN
Chinese Medical Journal 2007;120(11):1007-1009
Aged
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Atrial Flutter
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surgery
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Catheter Ablation
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adverse effects
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methods
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Cryosurgery
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adverse effects
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methods
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Female
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Humans
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Male
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Middle Aged
9.Histopathological changes post cryoablation in canine myocardium.
Jin-zhi FENG ; Fang WANG ; Gang CHEN ; Wei-dong MENG ; Ji-xiang XUE ; Bao-gui SUN
Chinese Journal of Cardiology 2006;34(9):829-832
OBJECTIVEThe purpose of this study was to observe histopathological changes post cryoablation in canine myocardium, to characterize the specific ablation lesion post cryoablation.
METHODSCryothermal ablation was applied on myocardium (both epicardium and endocardium) of 14 mongrel dogs with different ablation parameters (-25 degrees C x 4 min, -50 degrees C x 4 min, -75 degrees C x 4 min, -75 degrees C x 2 min, -75 degrees C x 6 min, -75 degrees C x 8 min). Lesion dimensions and histopathologic changes were observed.
RESULTSThe discrete, sharply delimited lesions were detected in cryoablated myocardium. Histologically, cryoablation in all temperatures studied induced heterogeneous necrosis of the myocardium. Lesion dimensions are related to freezing time and temperature.
CONCLUSIONCryoablation is a feasible and preferably choice for clinical application due to its controllable myocardium lesions.
Animals ; Catheter Ablation ; Cryosurgery ; Dogs ; Endocardium ; pathology ; Myocardium ; pathology
10.Endothelial progenitor cells related gene expression changes before and early after revascularization in patients with acute myocardial infarction.
Qiu-yan DAI ; Yi-wen YAN ; Zhi ZHANG ; Bao-gui SUN
Chinese Journal of Cardiology 2006;34(7):620-624
OBJECTIVEThe purpose of this study was to observe the endothelial progenitor cells (EPCs) related gene expression changes before and early after revascularization in patients with acute myocardial infarction.
METHODSPeripheral blood samples were taken from patients with acute anterior myocardial infarction 6 hours and 7 days after PCI and stenting. Mononuclear cells (MNCs) were isolated by Ficoll-density centrifugation and cultured in M-199 medium. After 14 days culture, attaching cells incorporated DiI-acetylated low-density lipoprotein (EPCs) were collected and RNA was isolated by Trizol for microarray analysis on 24 genes associated with permissibility/vessel tone (angiotensin system: ACE, AGTR-1, AGTR-2; NO system: eNOS; prostacyclin system: COX-2; endothelin system: ET-1, ETA, ETB; superoxide anions system: SOD-1), angiogenesis (adhesion molecule: CDH5; growth factors and receptors: VEGFR1, VEGFR2, VEGF) and endothelial cell activation (adhesion molecules expression: ICAM1, ICAM2, ICAM3, PECAM-1, E-Selectin, L-Selection, VCAM1; change phenotype from antithrombotic to prothrombotic: tPA, uPA, PAI, vWF). VEGFR2, PECAM-1 and VE-cadherin positive cells were identified by flow cytometry.
RESULTEight gene expressions (AGTR-1, AGTR-2, COX-2, eNOS, ET-1, ETA, VEGF) were significantly downregulated 7 days post PCI compared to pre-PCI (P < 0.05). Flow cytometry results showed that VEGFR2 positive cells were also significantly reduced post PCI than that of before PCI (P < 0.05).
CONCLUSIONPCI down-regulated endothelial progenitor cells related gene expressions in patients with acute myocardial infarction.
Aged ; Aged, 80 and over ; Centrifugation, Density Gradient ; Endothelial Cells ; cytology ; Endothelium, Vascular ; cytology ; Female ; Gene Expression ; Humans ; Male ; Middle Aged ; Myocardial Infarction ; metabolism ; therapy ; Myocardial Reperfusion ; Myocardial Revascularization ; Oligonucleotide Array Sequence Analysis ; Receptor, Angiotensin, Type 1 ; biosynthesis ; genetics ; Receptor, Endothelin A ; biosynthesis ; genetics ; Stem Cells ; cytology ; Superoxide Dismutase ; biosynthesis ; genetics