1.Clinjcopathological features of obesity-associated focal segmental glomerulosclerosis
Bao DONG ; Wen CHEN ; Hong CHENG
Chinese Journal of Nephrology 1997;0(06):-
Objective To elucidate clinical and pathological features of obesity-associated focalsegmental glomerulosclerosis(FSGS). Methods Clinicopathological data of thirteen obese cases (BMI3≥28kg/m2) with FSGS(OB-FSGS) and thirteen non-obese cases(BMI
4.Study on imaging parameters of multi-slice spiral CT long coverage angiography in intracranial and cervical arteries
Bao-Dong JIANG ; Xiao-Yuan FENG ; Cheng LIU ; Ke LI ; Fu-Hua YU ;
Chinese Journal of Radiology 2001;0(09):-
Objective To explore application of the bolus-triggered technique and optimal match between collimation and pitch of MSCTA in intracranial and cervical arteries.Methods(1)The small-dose tests were performed in 19 healthy volunteers and the theoretical threshold was obtained by the time-density curve.(2)forty healthy volunteers were divided randomly into two groups and the scanning parameters were as follows.Group A:collimation 1.0 mm,pitch 1.750. Group B:collimation 2.5 mm,pitch 0.625. Statistical significance was determined with the X~2 test and t test(?=0.05).Results(1)In 19 volunteers,CT value 4 seconds before the peak was 75 HU,the CT value at the beginning segment of the carotid artery and the C_1 segment of internal carotid artery was in accordance with the standard.(2)Image quality ofⅠ,Ⅱgrades artery structures of group B was superior to that of group A.Image quality ofⅢ,Ⅴ grades artery structures of group A was superior to that of group B.Conclusions(1)MSCTA in intracranial and cervical arteries can display systematically the cerebral and carotid arteries.(2)The bolus-triggered technique can improve the image quality of the target vessels.The image quality of the MSCTA of intracranial and cervical arteries is better with the threshold of 75 HU on the basis of 3.5 ml/s injection rate.(3)On the basis of the same other parameters,the optimal scanning parameters are a collimation of 1.0 mm with a pitch of 1.750.
5.Study on transdifferentiation of renal tubular epithelial cells in patients with chronic aristolochic acid nephropathy
Wen CHEN ; Yipu CHEN ; Jing ZHANG ; Hong CHENG ; Bao DONG ; Wanzhong ZOU
Chinese Journal of Nephrology 1997;0(01):-
Objective To investigate the relationship between tubular cells transdifferentiation and renal interstitial fibrosis in patients with chronic aristolochic acid nephropathy (CAAN). Methods Specimens from renal biopsies of 10 CAAN patients with serum creatinine level of (309. 41 ? 164. 44) ?mol/L were performed to examine the extent of renal interstitial fibrosis by Masson staining, the expression of collagen types Ⅰ and Ⅲ by sirius red staining, and the expression of cytokeratin(CK), ?-smooth muscle actin (a-SMA), vimentin (Vim) and transforming growth factor-? 1 (TGF-?1) by immunohistochemical staining. Quantitative analysis by computer image analytic system or semi-quantitative analysis were used to evaluate the data. Results There was positively significant correlation between interstitial collagen types Ⅰ and Ⅲ and serum creatinine level ( r =0. 890, P
6.Melanotic oncocytic metaplasia of the nasopharynx.
Bao-cheng DONG ; Hao TIAN ; Xin-qi JIA ; Gelin LI ; Cheng LU ; Yankun ZHANG ; Xiaoming ZHU ; Fang YU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(7):549-550
Aged
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Female
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Humans
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Male
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Melanosomes
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Metaplasia
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Middle Aged
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Nasopharynx
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cytology
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pathology
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Oxyphil Cells
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cytology
7.Results and analysis on the national key monitoring of endemic arsenism in Jilin Province in 2005 and 2006
Zhen-ming, LU ; Jian-dong, TONG ; Hai-tao, ZHANG ; Xiu-li, ZHANG ; Bao-cheng, LIANG ; Chun-yu, ZHANG
Chinese Journal of Endemiology 2009;28(5):551-553
Objective To explore the present condition of endemic arsenism, the implementation of control measures and the effect of the monitored county (Tongyu County) and the monitoring spot (Baiyintuhai Village) in 2006 and 2007. Methods According to the National Survey Scheme of Endemic Arsenism, the progress of anti-arsenic water in Tongyu, and the management and running of all engineering projects and the arsenic content in water were surveyed. The patients with endemic arsenism in Tongyu were generally surveyed. The arsenic content of the improved drinking water in Tongyu and the arsenic in urine of children aged 8-12 and adults over 18 years of age were determinted. The causes of resident death in the monitoring spot from the year of 2006 and 2007 were investigated. Arsenic content of drinking water and the urine of local residents was examined with "Model AFS-930 Double-Channel Atomic Fluorescence Spectrometer". Results There were 30 endemic arsenism areas, 157 areas with high arsenic content and all population of 57 576 in Tongyu. Six areas had improved water till 2006, where 20.0% of water had improved, and six water-improving projects were all running normally, benefiting a population of 1670. Eight high arsenic areas changed water, in a rate of 5.09%. Eight water-improving projects were functioning well, benefiting a population of 4350. Until 2007, 28 areas had improved water in Tongyu, accounting for 93.33%. These 28 projects were well running, covering a population of 7980. One hundred and fouty-eight high arsenic areas had changed water, reaching a rate of 94.27%. One hundred and fouty-eight projects changedg water were running normally, benefiting a population of 46 214. In the surveyed spots, arsenic content was between 0.004 mg/L and 0.005 mg/L in 2006 and between 0.010 mg/L and 0.021 mg/L in 2007, all in the normal range to the time being. The arsenic contents in urine of the children aged 8-12 in the monitoring spots were determinted, averaging at 0.024 mg/L in 15 samples, fluctuating between 0.005 mg/L and 0.048 mg/L in 2006. The average content in urine from adults was 0.019 mg/L in 53 samples, fluctuating between 0.005 mg/L and 0.087 mg/L in 2007. The arsenic contents in urine were all in the normal range in 2006 and 2007. In 2006 14 endemic arsenism patients, all in light symptoms, had been checked out, the morbidity being 6.19%. In 2007, 17 patients who were all in light symptoms were identified in a rate of 6.94%. There was no significant difference of morbidity between the two years(χ2=0.1059, P>0.05). Two patients died, unrelating with drinking high arsenic water in 2006 and 2007. Conclusions The prevention and control measures are well implemented in Tongyu. The water-improving projects are functioning well. The condition of endemic arsenism is slight and hasn't changed so much in these two years. The arsenic contents in urine of children and adults within the normal range, showing that improving water can control the occurrence and the development of endemic arsenism.
8.Endoscopic repair of nasal septal perforation with acellular dermal matrix and pedicled mucoperichondrial flap.
You-xiang MA ; An-zhou TAO ; Cheng LU ; Hao TIAN ; Bao-cheng DONG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2011;46(6):455-458
OBJECTIVETo introduce the method and evaluate the efficacy of endoscopic repair of nasal septal perforation with acellular dermal matrix and pedicled mucoperichondrial flap.
METHODSTwelve patients with perforation of nasal septum were encountered since February 2006 to October 2010. The most common symptoms and sings were nasal obstruction and crusting at the margin of the perforation. Eight of 12 patients were iatrogenic following surgery. The perforation typically located at anterior medial part of the nasal septum, with their sizes ranged approximately 1.0-2.3 cm in diameter. The incision was made at the anterior edge of the perforation from the left nasal cavity and continued to the nasal floor horizontally. It ended at the lateral nasal cavity. Then, another incision was made parallel to the first one, which was 1.5 cm from the posterior of the perforation. The two incisions was connected. The mucoperichondrium was stripped along with the incisions and the pedicle of mucoperichondrial flap kept on the nasal septum. Then, the flap was turned up to cover the perforation and fixed with apposition suture. Put the acellular dermal matrix graft on the perforation from the right nasal cavity and fixed it with apposition suture.
RESULTSThe healing of the acellular dermal matrix and mucoperichondrium was good in the first week postoperatively and there was no rejective reaction and contracture. The epithelization of the nasal septal perforation finished 4 weeks after surgery. Follow-up ranged from 3 months to 4 years. Eleven patients had successful outcomes with complete closure of their perforations. One patient failed the operation. All of them had no complications.
CONCLUSIONSUsing acellular dermal matrix graft and mucoperichondrial flap to repair the septal perforation is a simple method and the success rate is high. Therefore, it is an effective way to repair the perforation of nasal septum.
Adolescent ; Adult ; Aged ; Child ; Dermis ; transplantation ; Endoscopy ; Female ; Humans ; Male ; Middle Aged ; Nasal Mucosa ; transplantation ; Nasal Septal Perforation ; surgery ; Nasal Septum ; pathology ; surgery ; Otorhinolaryngologic Surgical Procedures ; methods ; Skin Transplantation ; Surgical Flaps ; Treatment Outcome ; Young Adult
9.A novel regulable sutureless aortic arch prosthesis:an experimental study
Qing-Qi HAN ; Zhi-Yun XU ; Bao-Ren ZHANG ; Sheng-Dong HUANG ; Lin HAN ; Fang-Lin LU ; Zhi-Gang SUN ; Dong ZHOU ; Hong-Cheng LIU ;
Academic Journal of Second Military Medical University 1999;0(12):-
Objective:To develop a novel regulable sutureless aortic arch prosthesis and to apply it in an animal experimental study.Methods:The arch skeleton of the prosthesis was made of tandem Z-shape NiTiNOL wire;the branch skeleton was made of laser-cut NiTiNOL tube;and the whole skeleton was coated with thin ePTFE film.The blood vessel was anastomosed by di- rect ligature,needing no manual suturing.The prosthesis was applied in swine aortic arch operations under the bypass condi- tion.The practicality for surgery and the feasibility of anastomosis of the prosthesis were assessed.Results:Aortic arch opera- tions were successfully performed in 6 of the 8 experimental animals.The prostheses were easy to use,and the mean bypass time was only 10 min.The blood loss of the anastomoses was less than 100 ml within 8 h postoperatively in 5 animals;one had more blood loss due to prosthesis mismatch.Conclusion:The novel regulable sutureless aortic arch prosthesis has satisfactory practicality for surgery and reliable anastomosis,making it promising in future clinical application.
10.Vitexicarpin, a flavonoid from Vitex trifolia L., induces apoptosis in K562 cells via mitochondria-controlled apoptotic pathway.
Hai-yan WANG ; Bing CAI ; Cheng-bin CUI ; Dong-yun ZHANG ; Bao-feng YANG
Acta Pharmaceutica Sinica 2005;40(1):27-31
AIMTo investigate the inhibitory effect of vitexicarpin on the proliferation of human cancer cells and its mechanism of action.
METHODSThe inhibitory effect of vitexicarpin on the proliferation of human cancer cells was evaluated by the SRB method and its apoptosis-inducing effect was demonstrated by morphological observation under light microscope, flow cytometric analysis and agarose gel electrophoresis. The proteins related to apoptosis were examined by Western blotting analysis.
RESULTSVitexicarpin significantly inhibited the proliferation of human cancer cells, A2780, HCT-15, HT-1080 and K562, with the IC50 values of (19.1 +/- 2.4) micromol x L(-1) for A2780(48 h), (0.66 +/- 0.10) micromol x L(-1) for HCT-15(48 h), (0.44 +/- 0.06) micromol x L(-1) for HT-1080 (48 h) and (0.28 +/- 0.14) micromol x L(-1) for K562 (24 h). The cells treated with vitexicarpin showed characteristic morphology typical for apoptosis and gave dose-dependent sub-G0/G1 peak in the flow cytometric analysis and DNA ladder on agarose gel electrophoresis. In Western blotting analysis, the cleavage of PARP and caspase-3, the release of cytochrome c from mitochondria into the cytosol, the decrease of Bcl-2 expression level, and the down-regulation of the ratio of Bcl-2/Bax expression level were examined in the K562 cells treated with vitexicarpin.
CONCLUSIONVitexicarpin induces apoptosis in K562 cells via mitochondria-controlled apoptotic pathway.
Antineoplastic Agents, Phytogenic ; pharmacology ; Apoptosis ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Female ; Flavonoids ; chemistry ; isolation & purification ; pharmacology ; Fruit ; chemistry ; Humans ; K562 Cells ; Mitochondria ; enzymology ; physiology ; Plants, Medicinal ; chemistry ; Vitex ; chemistry