Objective To establish a method for the determination of tetrah yd ropalmatione in FuLe granules. Methods A HPLC method was used with DiamonsilTM C 18(250? 4.6 mn, 5 ? m) column and a mobile phase of methanol- 0.1 % phospho ric acid solution (adjusted the pH to 6.0 with triethylamine)(60 ∶ 40). Flow ra te was 1.0 mL/min, column tem perature 35 ℃ , the detection wavelength at 280nm and sample loop volume bei ng 20 ? L. Results The calibration curve was linear within the range of 0.102 ～ 1.632 ? g of tetrahydropalmatione, the average recovery was 98.78 % (RSD=2 .75 % ).Conclusion The method is simple and accurate.It can be used for the q uality control of Fule granules.

Objective: To study the morphologic differences and nutritional components of the natural and broken Masson pine pollen. Methods: Pure pollen was collected from Masson pine (Pinus Massoniana Lamb) and the dry pollen was broken by high speed low temperature air flow technique. Their morphologic changes were observed under scan electronic microscopy. The nutritional components of pollen were analyzed.Results: The pollen particle consists of two air bags and one main part, like a mushroom cap. The main morphologic change of broken pollen was that the air bags were separated from pollen particles, and the main part of pollen particles became enriched. The physical characteristics of pollen were changed in container and its fluidity was lost. The broken pollen had very strong adhesiveness. So it could not fly like natural pollen. In broken pollen, the rough fiber, cellulose and semi cellulose contents decreased significantly. Total energy, total lipid, and starch contents increased remarkably. The water soluble and fat soluble components also increased greatly. The total amino acids content of broken pollen was higher than natural pollen except Cys, Arg and Met, and free amino acids were also higher except Cys, Gly and Met. In broken pollen, the amount of lipids extracted by ether increased sharply, nearly 7 times more than natural pollen.Conclusion: It is considered that some nutritional components of natural pollen can be released after broken. Because most of the lipids in pollen are easily oxidized, broken pollen can not be stored for long time.

Injunction is a conception of transactional analysis. It is an injunctive message delivered from parent to children. Injunction is important for development of personality. We suppose that a person who received more injunctions may tend to have more psychological problems. If chronic disease patients have some injunctions, they may show some psychological characteristics which are not conductive for rehabilitation. This article analyzed the injunctions in chronic disease patients and some coping methods were also dicussed.

Adolescent ; Autonomic Nervous System ; physiopathology ; Blood Pressure ; physiology ; Blood Pressure Monitoring, Ambulatory ; methods ; Child ; Child, Preschool ; Circadian Rhythm ; Female ; Heart Rate ; physiology ; Humans ; Male ; Posture ; physiology ; Syncope, Vasovagal ; diagnosis ; etiology ; physiopathology ; Tilt-Table Test ; Vagus Nerve ; physiopathology

Child ; Humans ; Inflammation ; Neoplasms, Muscle Tissue ; Ureteral Neoplasms

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Calcaneus ; Fasciitis, Plantar ; therapy ; Female ; Humans ; Male ; Middle Aged ; Musculoskeletal Manipulations ; Pain, Intractable ; therapy ; Pelvis ; injuries

Objective To approach effect of Yiqihuoxue decoction on remnant stomach paralysis syndrome after subtotal gastrectomy. Methods A total of 44 patient of remnant stomach paralysis syndrome were randomly recruited into a control group and a treatment group, with 22 patients in each group. The control group was treated with TPN parenteral alimentation. On this basis, the treatment group was treated with Yiqihuoxue decoction and mental intervention. The clinical effects were observed in both groups after the treatment. Results The total effective rate was 54.55% and 100% in the control group and the treatment group respectively, showing statistic difference(P=0.00625 ＜0.01).Conclusion It was effective to treat remnant stomach paralysis syndrome with Yiqihuoxue decoction, TPN parenteral nutrition and mental intervention.

Anemia, Hemolytic ; chemically induced ; therapy ; Female ; Humans ; Paraquat ; poisoning ; Poisoning ; therapy ; Young Adult

Objective Recent studies show that the PI3K/Akt signaling pathway is a key regulatory signal in both bone for-mation and bone remodeling .The experiment aimed to investigate the effects of inhibiting the PI 3K/Akt signaling pathway on the prolif-eration and cell cycles of MC3T3-E1 cells. Methods We treated MC3T3 -E1 cells with PF-04691502 the inhibitor of the PI3K/Akt signaling pathway .Then we determined the proliferative activity of the cells by MTS assay , detected the cell cycles by flow cytometry , and measured the expression of cell cycle-related proteins by Western blot . Results After inhibition of the PI3K/Akt signaling path-way with PF-04691502 at 30, 150 and 750 nmol/L, the proliferation of MC3T3-E1 cells was reduced in a dose-dependent manner to 0.647 ±0.041, 0.423 ±0.011 and 0.159 ±0.004, respectively, as compared with 1 ±0.056 in the control group (P<0.01).Sim-ultaneously , the percentage of the cells in the sub-G1 phase was significantly higher in the 1 μmol/L group than in the control ([1.45 ±0.43]%vs [0.27 ±0.21]%, P<0.01) and the expression of cyclin D1 was decreased. Conclusion Inhibition of the PI3K/Akt signaling pathway can block MC 3T3-E1 cells in the sub-G1 phase and reduce the proliferation of the cells .

ObjectiveTo compare and evaluate the effectiveness of Airtraq laryngoscope combined with a Bougie and Airtraq laryngoscope alone for tracheal intubation in simulated difficult airway.Methods Four anesthetists and 4 clinical physicians of standardized training were enrolled in the study.The participants intubated the trachea of the ALS simulator manikin in 5 tongue edema scenarios simulating modified Cormack-Lehane grade 1,2a,2b,3,and 4 views and 1 cervical immobilization scenario.Results No significant difference in the rate of successful intubation was detected between two techniques(P＞ 0.05 ).In Cormack-Lehane grade 1,2a views,the duration of successful intubation in Airtraq laryngoscope alone [ ( 14.3 ± 1.3),( 17.1 ± 2.9) s] was shorter than that in Airtraq laryngoscope combined with a Bougie [ (26.6 ± 3.8),(36.4 ± 3.6) s ] with significant difference (P ＜ 0.01 ).Cormack-Lehane grade 2b,3,4 views,the duration of successful intubation in Airtraq laryngoscope alone[ (74.5 ± 6.5 ),(116.3 ± 9.8),(53.0 ± 6.1 )s] was longer than that in Airtraq laryngoscope combined with a Bougie [ (35.4 ± 4.3 ),(52.3 ± 5.0),(40.4 ± 3.8 ) s ] with significant difference (P ＜ 0.05).ConclusionAirtraq laryngoscope combined with a Bougie can be quickly intubated in simulated difficult airway compared with Airtraq laryngoscope alone.