Grasses infected by endophytes have an extraordinary impact on the ecology and economy of pasture and turf. In this review, we presented recent research progress on origin and evolution, biological and ecological impact, and perspective of the association in order to make full use of the widely distributed endophyte resources.

Objective: To discuss the clinical significance of plasma von Willebrand factor (vWF), vascular endothelial growth factor (VEGF), and matrix metalloproteinase-9 (MMP-9) in patients with cerebral infarction in the process of occurrence and development of cerebral infarction. Methods: Thirty patients with cerebral infarction were divided into 3 groups according to their size of infarction: large infarction, moderate infarction, and small infarction (ten patients in each group). Enzyme-linked immunoadsordent assay (ELISA) was used to detect the concentrations of plasma vWF, VEGF, and MMP-9 in patient with cerebral infarction and in 20 healthy controls on day 1, 3, 7, and 15. Results: The mean levels of vWF (216 ± 62) μg/L, VEGF (584 ± 151) ng/ L, and MMP-9 (287 ± 147) μg/L in the cerebral infarction groups were significantly higher than those in the control group [vWF (96 ± 12) μg/ L, VEGF (111 ± 17) ng /L, and MMP-9 (102 ± 14) μg/L; P < 0.01]. The changes of plasma concentrations of the above 3 factors were correlated positively with the volume of cerebral infarction at the same time point in early cerebral infarction (r = 0.496, r = 0.519, r = 0.472 respectively; all P < 0.01). Conclusion: The concentrations of plasma vWF, VEGF, and MMP-9 were associated with the volume of cerebral infarction.

Objective To focus on the causes and management of complications after laparoscopic cholecystctomy (LC). Methods A retrospective analysis of clinical records of 13 278 patients undergoing LC in this hospital from March 1991 to July 2003 was made. Results Complications occurred in 110 patients (0.83%), involving 19 cases of bile duct injuries (0.14%), 37 cases of bile leakage (0.28%), 31 cases of residual calculi in common bile duct (0.23%), 4 cases of intraabdominal hemorrhage (0.03%), 5 cases of gastrointestinal injury (0.04%), 3 cases of intraabdominal abscess (0.02%), 6 cases of incisional infection or hernia (0.05%) and 5 cases of serious subcutaneous emphysema (0.04%), respectively. Five patients died (0.04%). Conclusions The major complications after LC include bile duct injury, bile leakage and residual calculus. Most of complications are preventable and curable.

Objective To investigate the causes,preventive measures and treatment of digestive tract injury during laparoscopic cholecystectomy (LC). Methods From January 2000 to December 2007,totally 21 640 patients underwent LC in our hospital,among them,16 had digestive tract injury including injuries in the stomach and duodenum in 14 patients,in the colon in 1,and in the ileum in 1. Results Among the 16 cases,13 were detected during the operation,and thus laparoscopic repair were performed on 3 of the cases,and conversion to open surgery were carried out for the other 10 for repair of the injured tract (7 cases) or major resection of the stomach and Roux-en-Y anastomosis (3 cases). The other 3 patients were confirmed after LC;they received open surgery for repair of the ileum,colostomy or intra-abdominal cavity drainage. In the 16 cases,one patient died of duodenal leakage complicated with multiple organ dysfunction syndrome in 7 days after the operation;the other 15 patients were cured and discharged from hospital without severe complications. Conclusions Most of the digestive tract injury cases during LC are caused by pericholecystic inflammatory adhesion,which leads to insufficient exposure of the surgical field resulting in the tear or perforation of the digestive tract. Proper treatment in early stage is the key to the prognosis of the patients and prevention of severe complications.

Identification and classification of white blood cells are important for clinical diagnosis.Many researchers have been seeking the effective methods for white blood cells' automatic classification based on morphological characters.After cell segmentation,leukocytes' feature acquirement and selection,this paper accomplishes white blood cells' automatic classification using Sugeno-model fuzzy neural network and compares the result with that from classifier of BP network.

0.05).Conclusion It is feasible to implement the DWS pulse sequence in conventional MR system to investigate the diffusion property of water molecule,furthermore the results are not dependent on diffusion weighting.

The treatment of cerebral palsy is still a big medical problem.The pathogenesis of spasticity,as result of a variety of lesions of the cerebral cortex,brain stem,spinal cord,is caused by involvement of the inhibitory pyramidal and parapyramidal descending tracts terminating on the spinal facilitatory myotatic reflex.A lesion of the descending tracts disturbs this equilibrium leading to spasticity,which is cha-racterized by muscle resistance at rest that is velocity dependent and associated with an increase in tonic stretch reflexes resulting from hype-rexcitability of the stretch reflex.Spasticity caused abnomal posture,caused special movement,and higher multilation,which affect children's life severely.There are so many ways to lower hypermyotonia,such as:drugs,rehabilitation care,acupuncture and so on.Bioelectric stimulation therapy is a new ways in the zone.Its curative effect and the mechanism are still in explore,this article just to give an overview about bioelectric stimulation therapy in curing spastic cerebral palsy.

BACKGROUND:With the prevalence of total hip arthroplasty,the demand for revision has been increased due to the prosthesis life and various complications.OBJECTIVE:To retrospectively analyze the reasons and clinical effectiveness of revision,clinical application of revision prosthesis,and related revision techniques.METHODS:Clinical data of 85 patients undergoing total hip revision were analyzed retrospectively.Among which,there were 23 cases of biological T.O.P acetabular cup (allograft bone graft) plus polyethylene liner,51 cases of titanium mesh cup plus bone cement fixation,and 11 cases of common acetabular cup;common hip stem in 63 cases (including 47 cases of bone cement fixation);lengthened hip stem in 34 cases (16 cases of modular prosthesis and 18 cases of bone cement fixation).Subsequently,the hip function was assessed by Harris hip scores.RESULTS AND CONCLUSION:All cases were followed up,and the mean follow-up was 30.2 months.(1) The average Harris hip scores changed from preoperative 30.07 to postoperative 90.32.The wound healed well,no dislocation or infection,and the hip function was improved significantly.(2) To conclude,aseptic loosening of the prosthesis is the main cause of reversion occurred,followed by prosthesis dislocation.Acetabular wall bone defect and decrease in bone mass are the main problems in revision.Adequate preoperative preparation for possible and unexpected events,and reasonable selection of prosthesis according to the bone defects will be helpful.Noticeably,early arthroplasty reversion will obtain good clinical efficacy.

Traditional Chinese medicine (TCM) emphasizes treatment based on syndrome differentiation, which em-bodies the concept of individualized diagnosis and dynamic treatment on a holistic view. RCT was frequently used in TCM clinical study. However, it cannot reflect the concept of treatment based on syndrome differentiation. The use of adaptive treatment strategies (ATS) in clinical research can adjust therapy in patients according to the change of disease, so it can reflect personalized medicine and dynamic treatment. From this perspective, the process of treat-ment based on syndrome differentiation was a process of adaptive treatment decision. So the method for developing ATS can effectively evaluate the efficacy of different treatment strategies in TCM clinical study. ATS had been used in some clinical studies from published literatures abroad. However, the use of ATS in domestic clinical research was seldom been reported. This paper gave an outline of the concept of ATS. In addition, this paper focused on three methods which can take the delayed effects of current treatment action into consideration and can compare the over-all effect of different ATS. It was hoped to lay foundation for future scientific research of TCM in the implementation of SMART and provide a new idea.