1.Study of Quality Standard for Shuangbai Quyu Powder
Zengxuan SONG ; Yuan CHEN ; Bin CHEN ; Bao YANG ; Hongmei LAN
China Pharmacist 2015;18(10):1819-1821
Objective:To study and promote the quality standard for Shuangbai Quyu powder. Methods:A microscopic identifi-cation method was performed to identify Phellodendri amurensis cortex, Rhei radix et rhizoma, Platycladi cacumen and Andrographis herba. TLC was performed to identify Phellodendri amurensis cortex, Andrographis herba and Rhei radix et rhizoma. HPLC was used to determine the content of berberine hydrochloride in Phellodendri amurensis cortex. and the lower limit was determined. Results: The microscopic identification method was simple. The spots in TLC were distinct without any interference from the negative coutrol. Ber-berine hydrochloride had a good linear range of 0. 010-0. 400 μg(r=0. 999 9). The average recovery was 99. 51%, and RSD was 0. 31% (n=6). Conclusion:The method can be used for the quality control of Shuangbai Quyu powder effectively,which is beneficial to the quality standard promotion of hospital preparations.
2.In situ lymphoma.
Ding-bao CHEN ; Lin DAI ; Song-lin LIAO
Chinese Journal of Pathology 2009;38(11):790-792
CD5 Antigens
;
metabolism
;
Chromosomes, Human, Pair 11
;
Chromosomes, Human, Pair 14
;
Chromosomes, Human, Pair 18
;
Cyclin D1
;
metabolism
;
Diagnosis, Differential
;
Humans
;
Ki-67 Antigen
;
metabolism
;
Lymphoma, B-Cell, Marginal Zone
;
genetics
;
metabolism
;
pathology
;
Lymphoma, Follicular
;
genetics
;
metabolism
;
pathology
;
radiotherapy
;
Lymphoma, Mantle-Cell
;
genetics
;
metabolism
;
pathology
;
Proto-Oncogene Proteins c-bcl-2
;
metabolism
;
Pseudolymphoma
;
metabolism
;
pathology
;
Translocation, Genetic
3.Richter syndrome: report of a case.
Ding-bao CHEN ; Qiu-jing SONG ; Dan-hua SHEN
Chinese Journal of Pathology 2010;39(7):487-488
Aged
;
Antibodies, Monoclonal, Murine-Derived
;
therapeutic use
;
Antigens, CD20
;
metabolism
;
Antineoplastic Agents
;
therapeutic use
;
Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
;
CD5 Antigens
;
metabolism
;
Cyclophosphamide
;
therapeutic use
;
Doxorubicin
;
therapeutic use
;
Humans
;
Leukemia, Lymphocytic, Chronic, B-Cell
;
drug therapy
;
metabolism
;
pathology
;
Lymphoma, Large B-Cell, Diffuse
;
drug therapy
;
metabolism
;
pathology
;
Male
;
Prednisone
;
therapeutic use
;
Receptors, IgE
;
metabolism
;
Rituximab
;
Vincristine
;
therapeutic use
4.Exploration of Rutual Recognition of Results of Protein and Lipid ExamInation among Various Clinical Laboratories
Dong LI ; Anyu BAO ; Lin SONG ; Zhen CHEN
Journal of Modern Laboratory Medicine 2015;(1):159-163
Objective To explore the possibility and reliability of mutual recognition of renal indexs among 20 clinical labora-tories entitled with state key clinical laboratory,and to supply reference for future national mutual recognition of laboratory examination results.Methods Determined the concentrations of TP,ALB,TC,TG and Roche multiple calibrators and sub-mitted the results.The results were analyzed for robust Z score,percentage difference after calibration,bias at medical deci-sion level to observe the possibility and reliability.Results The bias of TG was out of the least allowable bias,thus they were not appropriate to mutual recognition.Conclusion It remains immature in the mutual recognition of lipids’determina-tions and much work needs to be done in field of internal quality control of the laboratory.
5.Studies on the expression of type Ⅲ collagen in abdominal aortic aneurysm tissues and normal aortic tissues
Song NI ; Heng GUAN ; Yuehong ZHENG ; Bao LIU ; Hua CHEN ;
Chinese Journal of General Surgery 2001;0(09):-
Objective To investigate the expression of type Ⅲ collagen in abdominal aortic aneurysm tissues and normal aortic tissues. Methods RT PCR and immunohistochemistry were applied to detect the expression of type Ⅲ collagen in abdominal aortic aneurysm (AAA) tissues( n =5) and normal aortic (NA) tissues( n =3) . Results Expression of type Ⅲ collagen was increased in AAA group compared with normal group with AAA/NA= 7 251( P
6.Anaplastic large cell lymphoma of mixed sarcomatoid and giant-cell rich variant occurring in female external genitalia: report of a case.
Ding-bao CHEN ; Qiu-jing SONG ; Dong-mei BAO ; Dan-hua SHEN
Chinese Journal of Pathology 2006;35(12):759-760
Adult
;
Diagnosis, Differential
;
Female
;
Follow-Up Studies
;
Giant Cell Tumors
;
metabolism
;
pathology
;
surgery
;
Humans
;
Immunohistochemistry
;
Ki-1 Antigen
;
metabolism
;
Lymphoma, Large-Cell, Anaplastic
;
metabolism
;
pathology
;
surgery
;
Melanoma
;
pathology
;
Mucin-1
;
metabolism
;
Perineum
;
pathology
;
surgery
;
Protein-Tyrosine Kinases
;
metabolism
;
Receptor Protein-Tyrosine Kinases
;
Sarcoma
;
metabolism
;
pathology
;
surgery
7.Metastases of breast cancer to female genital tract: report of 2 cases.
Ding-bao CHEN ; Li-hua QIAN ; Qiu-jing SONG ; Dan-hua SHEN
Chinese Journal of Pathology 2006;35(3):190-191
Breast Neoplasms
;
metabolism
;
pathology
;
surgery
;
Carcinoma, Ductal, Breast
;
metabolism
;
secondary
;
surgery
;
Carrier Proteins
;
metabolism
;
Diagnosis, Differential
;
Endometrial Neoplasms
;
metabolism
;
secondary
;
Female
;
Glycoproteins
;
metabolism
;
Humans
;
Immunohistochemistry
;
Keratin-7
;
metabolism
;
Mastectomy, Modified Radical
;
Middle Aged
;
Ovarian Neoplasms
;
metabolism
;
secondary
8.Study on traceability system of genuine medicinal materials.
Bao-Sheng LIAO ; Jing-Yuan SONG ; Cai-Xiang XIE ; Jian-Ping HAN ; Shi-Lin CHEN
China Journal of Chinese Materia Medica 2014;39(20):3881-3888
Genuine medicinal materials with special characteristics of Traditional Chinese Medicine (TCM), is recognized as high quality medicine. Both ancient records and modern research considered that the origin is an important reason for the formation of genuine medicinal materials. However, blindly transplanting of genuine medicinal materials has led to the quality decline and counterfeit medicines appeared in production or sale progress, which may increase the risk of accidents in TCM. Frequent accidents emerged in Chinese herbal affects its export. What's more, it is a great threat to the medication safety in TCM clinical. There is an urgent need to implement traceability systems of TCM, which could provide convenient information record and traceability of TCM circulation. This paper reviews a variety of technical methods for genuine medicinal materials traceability, and proposed the establishment of genuine medicinal materials traceability system based on two-dimensional code and network database.
Databases, Factual
;
Drugs, Chinese Herbal
;
chemistry
;
economics
;
standards
;
Medicine, Chinese Traditional
9.Chemical constituents from Chenopodium ambrosioides.
Kun SONG ; Hong-Qing WANG ; Chao LIU ; Jie KANG ; Bao-Ming LI ; Ruo-Yun CHEN
China Journal of Chinese Materia Medica 2014;39(2):254-257
Twelve compounds were isolated from the herb of Chenopodium ambrosioides, and their structures were identified by spectroscopic methods as kaempferol-7-O-alpha-L-rhamnopyranoside (1), kaempferol-3,7-di-O-alpha-L-rhamnopyranoside (2), patuletin (3), quercetin-7-O-alpha-L-rhamnopyranoside (4), grasshopper ketone (5), 4-hydroxy-4-methyl-2-cyclohexen-1-one (6), syringaresinol (7), benzyl beta-D-glucopyranoside (8), dendranthemoside B (9), N-trans-feruloyl tyramine (10), N-trans-feruloyl 4'-O-methyldopamine (11), and 4-hydroxy-N-[2-(4-hydroxyphenyl) ethyl] benzamide (12). Among them,compounds 3, 6-8,10, and 12 were isolated from the genus Chenopodium for the first time, and compounds 2-12 were isolated from this plant for the first time.
Chenopodium ambrosioides
;
chemistry
;
Drugs, Chinese Herbal
;
chemistry
;
isolation & purification
10.Laparoscopic-assisted vaginoplasty by using sigmoid colon and peritoneum for congenital complete colpatresia
Bihui BAO ; Zhonglan XU ; Juan SONG ; Chuntao LIU ; Yong CHEN ; Huicheng XU ; Zhiqing LIANG ; Shuai TANG
Journal of Regional Anatomy and Operative Surgery 2016;25(4):266-269,270
Objective To study the desired therapeutic effect of vaginoplasty using sigmoid colon or peritoneum for entire vaginal atre-sia.Methods From May 2015 to October 2015,8 patients with complete vaginal atresia in our hospital performed vaginoplasty using sigmoid colon or peritoneum successfully.The operation methods,prognosis and therapeutic effect and quality of sexual life of two groups were com-pared and analyzed to investigate a perfect operative method and ideal nursing.Results Laparoscopic vaginoplasty using the peritoneum compared with that using sigmoid colon has advantages of shorter time of operation,less bleeding,less trauma and quicker recovery.However, artificial vagina using sigmoid colon was much more similar to a natural vagina in morphological and physiological characteristics,merely ac-companied by excessive mucus discharge during the postoperative period.At lower risk of vaginal adhesion and stenosis,laparoscopically as-sisted vaginoplasty using sigmoid colon graft also puts high technical requirements.Two patients normally developed uterus menstruate regular-ly.Four patients without uterus and 2 patients with rudimentary uterus experienced good sexual intercourse after vaginoplasty.Conclusion Patients with complete colpatresia who have normal womb should creat a patent and functional vagina until adolescence to restore its physio-logical and reproductive function.Anatomical reconstructive treatment of vaginal agenesis for patients with absent or hypoplastic uterus can be postponed till the late teens or in the adult.Both laparoscopic sigmoid vaginoplasty and peritoneal vaginoplasty achieve a minimal-invasive, cosmetic,natural lubricous, smooth artificial vagina for patient’ s sex life satisfactory.