1.Study of Quality Standard for Shuangbai Quyu Powder
Zengxuan SONG ; Yuan CHEN ; Bin CHEN ; Bao YANG ; Hongmei LAN
China Pharmacist 2015;18(10):1819-1821
Objective:To study and promote the quality standard for Shuangbai Quyu powder. Methods:A microscopic identifi-cation method was performed to identify Phellodendri amurensis cortex, Rhei radix et rhizoma, Platycladi cacumen and Andrographis herba. TLC was performed to identify Phellodendri amurensis cortex, Andrographis herba and Rhei radix et rhizoma. HPLC was used to determine the content of berberine hydrochloride in Phellodendri amurensis cortex. and the lower limit was determined. Results: The microscopic identification method was simple. The spots in TLC were distinct without any interference from the negative coutrol. Ber-berine hydrochloride had a good linear range of 0. 010-0. 400 μg(r=0. 999 9). The average recovery was 99. 51%, and RSD was 0. 31% (n=6). Conclusion:The method can be used for the quality control of Shuangbai Quyu powder effectively,which is beneficial to the quality standard promotion of hospital preparations.
2.In situ lymphoma.
Ding-bao CHEN ; Lin DAI ; Song-lin LIAO
Chinese Journal of Pathology 2009;38(11):790-792
CD5 Antigens
;
metabolism
;
Chromosomes, Human, Pair 11
;
Chromosomes, Human, Pair 14
;
Chromosomes, Human, Pair 18
;
Cyclin D1
;
metabolism
;
Diagnosis, Differential
;
Humans
;
Ki-67 Antigen
;
metabolism
;
Lymphoma, B-Cell, Marginal Zone
;
genetics
;
metabolism
;
pathology
;
Lymphoma, Follicular
;
genetics
;
metabolism
;
pathology
;
radiotherapy
;
Lymphoma, Mantle-Cell
;
genetics
;
metabolism
;
pathology
;
Proto-Oncogene Proteins c-bcl-2
;
metabolism
;
Pseudolymphoma
;
metabolism
;
pathology
;
Translocation, Genetic
3.Exploration of Rutual Recognition of Results of Protein and Lipid ExamInation among Various Clinical Laboratories
Dong LI ; Anyu BAO ; Lin SONG ; Zhen CHEN
Journal of Modern Laboratory Medicine 2015;(1):159-163
Objective To explore the possibility and reliability of mutual recognition of renal indexs among 20 clinical labora-tories entitled with state key clinical laboratory,and to supply reference for future national mutual recognition of laboratory examination results.Methods Determined the concentrations of TP,ALB,TC,TG and Roche multiple calibrators and sub-mitted the results.The results were analyzed for robust Z score,percentage difference after calibration,bias at medical deci-sion level to observe the possibility and reliability.Results The bias of TG was out of the least allowable bias,thus they were not appropriate to mutual recognition.Conclusion It remains immature in the mutual recognition of lipids’determina-tions and much work needs to be done in field of internal quality control of the laboratory.
4.Richter syndrome: report of a case.
Ding-bao CHEN ; Qiu-jing SONG ; Dan-hua SHEN
Chinese Journal of Pathology 2010;39(7):487-488
Aged
;
Antibodies, Monoclonal, Murine-Derived
;
therapeutic use
;
Antigens, CD20
;
metabolism
;
Antineoplastic Agents
;
therapeutic use
;
Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
;
CD5 Antigens
;
metabolism
;
Cyclophosphamide
;
therapeutic use
;
Doxorubicin
;
therapeutic use
;
Humans
;
Leukemia, Lymphocytic, Chronic, B-Cell
;
drug therapy
;
metabolism
;
pathology
;
Lymphoma, Large B-Cell, Diffuse
;
drug therapy
;
metabolism
;
pathology
;
Male
;
Prednisone
;
therapeutic use
;
Receptors, IgE
;
metabolism
;
Rituximab
;
Vincristine
;
therapeutic use
5.Studies on the expression of type Ⅲ collagen in abdominal aortic aneurysm tissues and normal aortic tissues
Song NI ; Heng GUAN ; Yuehong ZHENG ; Bao LIU ; Hua CHEN ;
Chinese Journal of General Surgery 2001;0(09):-
Objective To investigate the expression of type Ⅲ collagen in abdominal aortic aneurysm tissues and normal aortic tissues. Methods RT PCR and immunohistochemistry were applied to detect the expression of type Ⅲ collagen in abdominal aortic aneurysm (AAA) tissues( n =5) and normal aortic (NA) tissues( n =3) . Results Expression of type Ⅲ collagen was increased in AAA group compared with normal group with AAA/NA= 7 251( P
6.Anaplastic large cell lymphoma of mixed sarcomatoid and giant-cell rich variant occurring in female external genitalia: report of a case.
Ding-bao CHEN ; Qiu-jing SONG ; Dong-mei BAO ; Dan-hua SHEN
Chinese Journal of Pathology 2006;35(12):759-760
Adult
;
Diagnosis, Differential
;
Female
;
Follow-Up Studies
;
Giant Cell Tumors
;
metabolism
;
pathology
;
surgery
;
Humans
;
Immunohistochemistry
;
Ki-1 Antigen
;
metabolism
;
Lymphoma, Large-Cell, Anaplastic
;
metabolism
;
pathology
;
surgery
;
Melanoma
;
pathology
;
Mucin-1
;
metabolism
;
Perineum
;
pathology
;
surgery
;
Protein-Tyrosine Kinases
;
metabolism
;
Receptor Protein-Tyrosine Kinases
;
Sarcoma
;
metabolism
;
pathology
;
surgery
7.Chemical constituents from Chenopodium ambrosioides.
Kun SONG ; Hong-Qing WANG ; Chao LIU ; Jie KANG ; Bao-Ming LI ; Ruo-Yun CHEN
China Journal of Chinese Materia Medica 2014;39(2):254-257
Twelve compounds were isolated from the herb of Chenopodium ambrosioides, and their structures were identified by spectroscopic methods as kaempferol-7-O-alpha-L-rhamnopyranoside (1), kaempferol-3,7-di-O-alpha-L-rhamnopyranoside (2), patuletin (3), quercetin-7-O-alpha-L-rhamnopyranoside (4), grasshopper ketone (5), 4-hydroxy-4-methyl-2-cyclohexen-1-one (6), syringaresinol (7), benzyl beta-D-glucopyranoside (8), dendranthemoside B (9), N-trans-feruloyl tyramine (10), N-trans-feruloyl 4'-O-methyldopamine (11), and 4-hydroxy-N-[2-(4-hydroxyphenyl) ethyl] benzamide (12). Among them,compounds 3, 6-8,10, and 12 were isolated from the genus Chenopodium for the first time, and compounds 2-12 were isolated from this plant for the first time.
Chenopodium ambrosioides
;
chemistry
;
Drugs, Chinese Herbal
;
chemistry
;
isolation & purification
8.Short-term results of cutting balloon and intravascular brachytherapy for the treatment of coronary in-stent restenosis
Fei YE ; Shao-Liang CHEN ; Bao-Xiang DUAN ; Jin HUANG ; Zhi-Zhong LIU ; Jie SONG ;
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
0.05); the levels of LL, LI, RRS in CBA group and CBA+IBT group were significantly lower than those in control group(P
9.Surface landmark of internal jugular vein and carotid artery in subtemporal para pharyngeal region
Jiuyu SONG ; Zequan HUA ; Li ZHANG ; Haihong BAO ; Lina WANG ; Zhihong CHEN
Journal of Practical Stomatology 2000;0(05):-
Objective: To study surface landmark of the blood vessles i n subtemporal parapharyngeal region. Methods:6 corpse heads were dissected, the anatomical character and the ralationship between surface landma rks and blood vessels was observed. Results:The distance between inferior medial point of meatus acusticus externus and internal jugular vein fo ramen was (11.5?2.0) mm, that between medial margin of condyle process and in ternal artery foramen was (10.0?1.3) mm. Internal carotid artery was (12.0? 3.0) mm away from posterior margin of mandibular horn, internal jugular vein wa s (5.0?2.0) mm in front of the transverse process of the first cervical verte brae (S1).Conclusions:The inferior point of meatus acustics exte rnus, anterior point of condyle process, mandibular horn and transverse process of S1 can be used as surface landmarks for internol jugular vein and carotid art ery.
10.Association of genetic polymorphisms in the DNA repair gene XRCC1 and XPD with risk of prostate cancer
Jie LIU ; Bao SONG ; Hong WANG ; Jun TIAN ; Zhen CHEN ; Huan SHI ; Zhehai WANG
Chinese Journal of Urology 2009;30(12):834-837
Objective To explore the relationship between DNA repair gene XRCC1 and XPD polymorphisms and individual susceptibility to prostate cancer. Methods PCR-restriction fragment length polymorphism assay was used to analyze the XRCC1 (C26304T and G28152A) and XPD A35931C polymorphisms in 358 prostate cancer patients and 312 healthy controls. Unconditional logistic regression analysis was performed to calculate odd ratio (OR) and 95% confidence interval (CD for estimating the correlation between different genotypes and prostate cancer risks. Results Forty-seven(13.1%) cases present XRCC1 28152AA genotype in prostate cancer group, while 24 cases in the control group (7. 1%), individuals with this genotype had a significantly increased risk for prostate cancer (OR 1. 924, 95%CI=1.126 - 3. 288, P=0. 017). There was no significant difference between two groups at XRCC1 C26304T and XPD A35931C sites. Combined analysis of the three sites polymorphisms showed that individuals with XRCC1 28152 AA and XPD 35931AC+CC genotype had a higher risk of prostate cancer than those with three wild genotypes (OR = 3. 087,95%CI 1. 081 - 8.813;OR = 3. 376,95%CI 1.067-10.683;OR 3. 216,95%CI=1. 439-7.188,P = 0. 004). Analysis stratified by age of onset, PSA, Gleason score and T stage revealed that XRCC1 28152AA and XPD 35931 AC+CC high-risk genotype was especially associated with early age at onset of prostate cancer (P<0. 05). Conclusions The XRCC1 and XPD genotypes may be contributed to the risk of developing prostate cancer, particularly for younger patients.