The H2O2 has been shown to have comprahensive actions on various ion channels of cell membrane. But it has different effects on different tissues. H2O2-induced a dose dependent decreasing L- type Ca2+ current on cultrued Lymnaea neurons. In sheep cardiac SR, H2O2 plays an important role in regulating directly on RyR Ca2+-release channel that caused an increasing of P0, but it was no effects on conductance. It indicated that H2O2-induced different activities on KCa channel with different tissues. In guinea pig ventricular myocytes, H2O2-induced increase in KATP channels. There are few data on the effects of H2O2 on whole cell Na+ currents and single Na+ channels.

Adult ; Aged ; Benzidines ; toxicity ; Coloring Agents ; Humans ; Male ; Middle Aged ; Occupational Exposure ; Urinary Bladder Neoplasms ; epidemiology

Accidents, Occupational ; Acrylamide ; poisoning ; Adolescent ; Critical Illness ; Humans ; Male ; Young Adult

Humans ; Hydrogen Sulfide ; poisoning ; Male ; Methylene Blue ; therapeutic use ; Poisoning ; therapy ; Young Adult

Prostate cancer is one of the common malignant tumors of the urinary system and mostly found in elderly men. Like most tumors, prostate cancer involves a variety of molecules in its occurrence and progression. More studies on the development of prostate cancer focus on the tumor markers, DNA damage repair related genes, and tumor invasion and metastasis related factors. This article presents an overview on the research progress in these three aspects.
Biomarkers, Tumor ; Biomedical Research ; DNA Repair ; Disease Progression ; Humans ; Male ; Neoplasm Invasiveness ; Prostatic Neoplasms ; genetics ; pathology

Animals ; Body Water ; Brain ; anatomy & histology ; Male ; Organ Size ; Rats ; Rats, Wistar ; Specific Gravity

Angiogenesis Inhibitors ; pharmacology ; Angiostatins ; pharmacology ; Animals ; Antigens, CD ; metabolism ; Cadherins ; metabolism ; Humans ; Matrix Metalloproteinase Inhibitors ; Matrix Metalloproteinases ; metabolism ; Microvessels ; metabolism ; Neoplasms ; blood supply ; pathology ; Neovascularization, Pathologic ; metabolism ; pathology ; Vascular Endothelial Growth Factor A ; metabolism

Adrenal Cortex Hormones ; therapeutic use ; Adrenergic beta-Agonists ; therapeutic use ; Anti-Asthmatic Agents ; therapeutic use ; Asthma ; drug therapy ; Humans ; Medication Adherence

Objective To investigate the expression of T help cell 1(Th1),T help cell 2(Th2)and related cytokines interleukin 12 (IL -12)and interleukin 4 (IL -4)in the lumbar disc herniation and their clinical significance. Methods 30 postoperative patients with lumbar disc herniation were included as observation group.The control group included 10 patients with lumbar fracture.Flow cytometry was used to distinguish the Th1 and Th2 cells.ELISA was used to measure the expression of interferon γ(IFN -γ),IL -12(the cytokines secreted by Th1 cells)and IL -4 (the cytokine secreted by Th2 cells).RT -PCR was used to detect the mRNA levels of STAT4 and STAT6.Results The distribution of Th1 /Th2 cells was higher in patients with lumbar disc herniation compared to the control group [(6.24 ±2.89)vs (25.12 ±3.20),t =16.26,P <0.05;(2.68 ±0.58)vs (8.16 ±1.20),t =3.84,P <0.05]. The levels of IFN -γand IL -12 in the study group were significantly higher than the control group,the differences were statistically significant[(23.47 ±5.61)vs (7.65 ±3.21),t =8.422,P <0.05;(1.52 ±0.87)vs (5.34 ± 1.39),t =8.135,P <0.05].The level of IL -4 was undetectable in the control group,however,IL -4 expressed in the study group.The expressions of IL -12,IFN -γand IL -4 in the study group were negatively correlated by Spearman correlation analysis (r =-0.57,P <0.05;r =-0.23,P <0.05).In addition,the mRNA level of STAT4 was higher in patients with lumbar disc herniation compared to the control group[(3.21 ±0.49)vs (1.12 ±0.24), t =13.15,P <0.05].Conclusion The expressions of Th1 cells,Th2 cells and related cytokines were participated in the pathogenesis of lumbar disc herniation.

Objective: To induce human dental mesenchymal cells to differentiate into odontoblasts in vitro.Methods:The cultured human dental mesenchymal cells were induced in two-dimensional culture model by bFGF(10 ng/ml)+IGF-1(100 ng/ml) or TGF-?1(5 ng/ml) for 4-7 d. Cell growth and morphology after induction were observed. The expression of human DSP protein and DSPP mRNA were detected by immunofluorescent staining and RT-PCR. Mineralization capability of the induced cells was evaluated using Von Kossa staining. Results:In both bFGF+IGF and TGF-?1 groups 20%-30% of the induced cells showed long single process.DSP protein and DSPP mRNA were observed in the induced cells.Mineralized nodules were found in the induction cultures.Conclusion: bFGF+IGF-1 or TGF-?1 can induce dental mesenchymal cells to differentiate into odontoblasts.