1.A case of neonatal protein C deficiency.
Yu BAO ; Li-ping SHI ; Xiu-jing WU
Chinese Journal of Pediatrics 2011;49(5):390-391
2.Study on the Chemical constituents of a Traditional Mogolian Medicine HERBA CLEMATIS
Baoquan BAO ; Yahan WU ; Jianping CHEN ; Ren BU ; Na BAO ; Ping ZHANG
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(9):1929-1933
This study was aimed to isolate and characterize of chemical constituents in a traditional Mongolian medicine HERBA CLEMATIS. Normal and reverse phase coloumn chromatography, gel filtration chromatography sephadex LH-20, and preparative HPLC were used for isolation and purification compounds from the water extraction of the arial part ofC. aethusaefoliaTurcz. The planar structures and spatial configurations of isolated compounds were identified by high resolution MS, 1D and 2D NMR, and other spectrographic methods. The chemical research on the Mongolian medicine results 6 compounds, dihydrodehydrodiconiferyl alcohol (1), syringaresinol (2), pinoresinol (3), epi-pinoresinol (4), lirioresinol B dimethyl ether (5) and loliolide (6). All the compounds were isolated from this plant for the first time.
3.Detection of the anterior descending artery originating from the right coronary artery in tetralogy of Fallot by echoeardiography
Chunhua ZHENG ; Min BAO ; Shaobo WANG ; Xiangjun LIU ; Xiujie TANG ; Ping LU ; Hongyin LI ; Qingyu WU
Chinese Journal of Ultrasonography 2009;18(8):669-671
malities should be alerted in TOF.
4.Operation performance of membrane nitrification bioreactor for ammonia-containing wastewater.
Xiao-Ying WU ; Ping ZHENG ; Bao-Lan HU
Chinese Journal of Biotechnology 2005;21(2):279-283
The operation performance of membrane nitrification bioreactor to treat ammonia-containing wastewater as well as the capabilities of separation and filtration of polypropylene membrane modules were tested. The removal efficiency of ammonia was kept higher than 95% when the hydraulic retention time was set at 1 day, the influent concentration was increased up to 80 mmol (NH4+ -N) x L(-1) and the volume loading rate was increased up to 1.12 kg (NH4+ -N) x m(-3) x d(-1). The biomass in the reactor was accumulated from 5 g x L(-1) to 10 g x L(-1) within 50 days, which indicated that polypropylene membrane modules were efficient in retaining biomass. The biomass attached to the membrane also contributed to the conversion of ammonia and nitrite. When the hydraulic pressure was lower than 80cm, the increase of hydraulic pressure improved the permeation of membrane. However, when the hydraulic pressure was beyond 80cm, the increase of hydraulic pressure did not significantly improve the permeation of membrane. The permeate flux was the highest 2.51 (L x m (-2) x h(-1)) but the resistance was the lowest (2.63 x 10(-5)) m(-1) when the hydraulic pressure was about 20 cm. The results showed that the membrane nitrification bioreactor could be run normally without extra energy input.
Biodegradation, Environmental
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Bioreactors
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microbiology
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Membranes, Artificial
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Nitrobacter
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growth & development
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metabolism
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Polypropylenes
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Quaternary Ammonium Compounds
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metabolism
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Waste Disposal, Fluid
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methods
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Water Pollutants, Chemical
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metabolism
5.99mTc-TRODAT-1 SPECT imaging of dopamine transporter in early diagnosis of Parkinsons disease.
Weifeng LUO ; Shiyao BAO ; Jinchang WU ; Yu FU ; Chunfeng LIU ; Guofu SHAO ; Ping FANG
Chinese Journal of Nervous and Mental Diseases 2001;27(2):106-108
Objective To evaluate the value of 99m Tc-TRODAT-1 SPECT DAT imaging in the early diagnosing of Parkinsons disease (PD).Methods Eleven patients (9 PD and 2 possible PD) and eighteen healthy subjects matched by sex and age were studied with 99m Tc-TRODAT-1 SPECT DAT imaging. Striatum specific uptake of 99mTc-TRODAT-1 was calculated according to the ratio of DAT uptake in striatum (ST) and cerebellum (CB). Results In the hemi-Parkinsons disease group, the DAT specific uptake of 99mTc-TRODAT-1 was significantly lower (P<0.01) in contralateral than in ipsilateral striatum to the clinically symptomatic side. There was significant decrease (p<0.01) of striatum DAT uptake in patients with hemi-PD compared to the controls.Conclusions 99mTc-TRODAT-1 SPECT DAT imaging may help to confirm the diagnosis of PD at the early stage.
6.Temporary interventional pneumonectomy used as an emergency treatment for acute massive pulmonary embolism: the initial experimental results
Yong FAN ; Yang LIU ; Qi WU ; Ping LI ; Jing TIAN ; Guijun BAO ; Nengshu HE
Journal of Interventional Radiology 2009;18(12):916-919
Objective To evaluate regional airway obstruction with balloon catheter in stabilizing the vital signs in experimental animals suffered from acute massive pulmonary embolism. Methods Pulmonary embolism of right lung artery by using auto-blood clots or detachable latex balloons was established in 27 healthy sheep. When the blood oxygen saturation decreased by 25% compared to that before the procedure,the placement of balloon catheter in corresponding right main bronchus was carried out in 18 sheep (study group). Five sheep were used as control group. The blood oxygen saturation in the remaining four sheep did not reach the intervention level. The pulmonary arterial pressure, the peripheral arterial pressure, the central venous pressure, the heart rate, the blood oxygen saturation, the arterial partial pressure of oxygen as well as of carbon dioxide were invasively determined. Results The experimental model of acute massive pulmonary embolism was successfully established in 23 sheep. After the establishment of pulmonary embolism, increased heart rate, tachypnea, a decrease of ≥ 25% in blood oxygen saturation within 30 minutes and a rise in pulmonary arterial pressure were detected. After the placement of balloon catheter in study group, a reduction of the pulmonary arterial pressure and an elevation of the blood oxygen saturation as well as the arterial partial pressure of oxygen rose. were observed. The difference between study group and control group was statistically significant (P < 0.05). Conclusion The result of this animal experiment indicates that obstruction of airway with balloon can be served as a first aid for acute massive pulmonary embolism, which can stabilizes the animal's vital signs and, therefore, can help gain precious time for the follow-up thrombolysis treatment.
7.Effects of different doses of topiramate on expression of NCAM and GAP- 43 mRNA in hippocampus of rats with epilepsy
Danhong WU ; Ping ZHONG ; Guanshui BAO ; Hong ZHOU ; Jun SHEN ; Feifei HUANG
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(12):1459-1462
Objective To explore the effects of different doses of topiramate (TPM) on the expression of nerve cell adhesion molecule (NCAM) and growth-associated protein 43 (GAP- 43) mRNA in hippocampus of rats with epilepsy. MethodsForty-eight rats were randomly divided into normal control group, kainic acid (KA) group, 10 mg/kg TPM group, 40 mg/kg TPM group, 100 mg/kg TPM group and 400 mg/kg TPM group (n=8). The models of rats with epilepsy treated by different doses of TPM were established. The behavior of rats was observed, and the expression of NCAM and GAP- 43 mRNA in hippocampus of rats was determined by Real-time PCR. Results The expression of NCAM and GAP- 43 mRNA in KA group was significantly higher than that in normal control group (P<0.01), while there was no significant difference between 10 and 40 mg/kg TPM groups and KA group, that in 100 and 400 mg/kg TPM groups was significantly lower than that in KA group (P<0.01), and that in 400 mg/kg TPM group was significantly lower than that in 100 mg/kg TPM group (P<0.01). Conclusion KA can up-regulate the expression of NCAM and GAP- 43 mRNA in hippocampus of rats with epilepsy. Higher dose of TPM can inhibit the expression of NCAM and GAP- 43 mRNA, and the inhibitory effect is related with the dose of TPM.
8.Temporary interventional pneumonectomy used as an emergency treatment for acute massive pulmonary embolism:the initial experimental results
Yong FAN ; Yang LIU ; Qi WU ; Ping LI ; Jing TIAN ; Guijun BAO ; Nengshu HE
Journal of Interventional Radiology 2006;0(12):-
Objective To evaluate regional airway obstruction with balloon catheter in stabilizing the vital signs in experimental animals suffered from acute massive pulmonary embolism.Methods Pulmonary embolism of right lung artery by using auto-blood clots or detachable latex balloons was established in 27 healthy sheep.When the blood oxygen saturation decreased by 25%compared to that before the procedure, the placement of balloon catheter in corresponding right main bronchus was carried out in 18 sheep(study group).Five sheep were used as control group.The blood oxygen saturation in the remaining four sheep did not reach the intervention level.The pulmonary arterial pressure,the peripheral arterial pressure,the central venous pressure,the heart rate,the blood oxygen saturation,the arterial partial pressure of oxygen as well as of carbon dioxide were invasively determined.Results The experimental model of acute massive pulmonary embolism was successfully established in 23 sheep.After the establishment of pulmonary embolism,increased heart rate,tachypnea,a decrease of≥25%in blood oxygen saturation within 30 minutes and a rise in pulmonary arterial pressure were detected.After the placement of balloon catheter in study group,a reduction of the pulmonary arterial pressure and an elevation of the blood oxygen saturation as well as the arterial partial pressure of oxygen rose were observed.The difference between study group and control group was statistically significant(P
9.Cyclin D1b Splice Variant Promotes αvβ3-mediated EMT Induced by LPS in Breast Cancer Cells
Bao-Ping LUO ; Jing LUO ; Yi-Bing HU ; Xiao-Wei YAO ; Feng-Hua WU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2018;38(3):467-472
Epithelial-to-mesenchymal transition (EMT) plays a critical role in cancer metastasis,and is relevant to the inflammatory microenvironment.Lipopolysaccharide (LPS),a cell wall constituent of gram-negative bacteria,has been reported to induce EMT of cancer cells through TLR4 signal.We previously reported that LPS promoted metastasis of mesenchymallike breast cancer cells with high expression of cyclin D 1 b.However,the role of cyclin D1b in LPS-induced EMT has not been fully elucidated.In the present study,we described that cyclin D1b augmented EMT induced by LPS in MCF-7 breast cancer cells.Cyclin D1b markedly amplified integrin αvβ3 expression,which was further up-regulated under LPS stimulation.Our results showed ectopic expression of cyclin D1b promoted invasiveness of epithelial-like MCF-7 cells under LPS stimulation.Additionally,LPS-induced metastasis and EMT in MCF-7-D1b cells might depend on αvβ3 expression.Further exploration indicated that cyclin D1b cooperated with HoxD3,a transcription factor promoting αvβ3 expression,to promote LPS-induced EMT.Knockout of HoxD3 repressed LPS-induced EMT and αvβ3 over-expression in MCF-7 cells with high expression of cyclin D1b.Specifically,all these effects were in a cyclin D1a independent manner.Taken all together,LPS up-regulated integrin αvβ3 expression in MCF-7 cells with high expression of cyclin D 1b and induced EMT in breast cancer cells,which highlights that cyclin D1b may act as an endogenous pathway participating in exogenous signal inducing EMT in breast cancer cells.
10.Association of peripheral blood estradiol, progesterone and testosterone levels with irritable bowel syndrome.
Nan CUI ; Bao-ping WU ; Sai-zhu WU
Journal of Southern Medical University 2006;26(3):367-368
OBJECTIVETo investigate the relation of peripheral blood estradiol, progesterone and testosterone levels with irritable bowel syndrome (IBS).
METHODSForty-eight patients with IBS identified according to Rome II diagnostic criteria and 30 healthy subjects as controls were analyzed for peripheral blood sex hormone levels by radioimmunoassay and corresponding software.
RESULTSIn male patients with IBS, blood testosterone level was significantly lower than that of the control group (P<0.05), but blood estradiol and progesterone showed no significant differences between the two groups (P>0.05). In the female patients, blood estradiol level was significantly lower than that of the control group (P<0.05), whereas blood progesterone and testosterone levels had no significant differences between the two groups (P>0.05).
CONCLUSIONPeripheral blood testosterone level in male IBS patients and estradiol level in female patients are lower than those of healthy subjects, suggesting that IBS might be associated with blood sex hormone disorder.
Adult ; Aged ; Estradiol ; blood ; Female ; Humans ; Irritable Bowel Syndrome ; blood ; Male ; Middle Aged ; Progesterone ; blood ; Radioimmunoassay ; Testosterone ; blood