1.The Random Amplified Polymorphic DNA Identification of 9 Taenia saginata Isolates from Four Provinces
Ke ZHANG ; Ming YANG ; Huaien BAO
Chinese Journal of Parasitology and Parasitic Diseases 1997;0(06):-
Objective To make molecular identification for 9 isolates of Taenia saginata from 4 provinces.Methods Genomic DNA was extracted from the segments of adult tapeworms collected from Taoyuan of Taiwan(TW1),Duyun of Guizhou(DY1,DY2),Congjiang of Guizhou(CJ1,CJ2,CJ3,CJ4),Dali of Yunnan(DL1) and Wushi of Xinjiang(XJ1) respectively.PCRs were carried out with 13 random primers.A phylogenetic tree of different geographical strains was constructed.Results 331 DNA fragments were amplified.The number of DNA fragments amplified by single primer was between 3 and 28.The average number of amplified DNA fragments by the 13 primers was 14.15.The average number of fragments from the 9 isolates of T.saginata was 14.08.Phylogenetic tree revealed that there were two branches in the tree,DY1,DY2,DL1 and TW1 occupied one branch,while CJ1,CJ2,CJ3,CJ4 and XJ1 occupied the other one.Conclusions By the RAPD analysis,the isolates DY1,DY2,DL1 and TW1 belong to Taenia saginata asiatica,and the isolates CJ1,CJ2,CJ3,CJ4 and XJ1 belong to T.saginata saginata.
3.The risk factors of severe acute pancreatitis complicated with multiple organ dysfunction syndrome(MODS)in the early stage
Bao LIU ; Aijun PAN ; Ming SHAO ; Linlin ZHANG ; Shusheng ZHOU
Chinese Journal of Emergency Medicine 2006;0(02):-
Objective To study the risk factors of severe acute pancreatitis(SAP) complicated with MODS at the early stage.Methods One hundred and seven SAP patients served as A group,who admitted from Janurary 1995 to December 1999.One hundred and thirty patients served as B group who admilted from Janurary 2000 to 2005.Age,sex,Ranson' score,APACHEⅡ ,CT score,biliary tract obstruction,hypoxia,lung infection,shock,abdomen compartment syndrome,hyperlipemia,pleural effusion,and mortality were analyzed.Results Twenty-five in the A group and 28 in B group two were complicated with MODS at the early stage.There existed difference in Ranson's score,APACHEⅡ,CT score between patients with and without MODS(P
4.Effect of oxidized low-density lipoprotein on endothelium-dependent relaxation in hyperlipidemic patients
Jibing QU ; Xueying BAO ; Ming ZHANG ; Hongwei DU
Journal of Jilin University(Medicine Edition) 2006;0(06):-
Objective To study the effecet of oxidized low-density lipoprotein(Ox-LDL) on endothelium-dependent relaxation and mechanism of susceptibility to atherosclerosis(AS) in hyperlipdemic male patients.(Methods)LDL isolated from 13 normal patients and 29 hyperlipidemic patients were modified by CuSO_4.The amount of malondialdehyde(MDA) was measured by TBARS.The amount of lysophosphatidylcholine(LPC) was determined by the Bartlett.Endothelium-dependent relaxation was produced by acetylcholine.Results After LDL from normal and hyperlipidemic patients were modified by CuSO_4,the amount of MDA was increased(P
5.Value of double stapling technique with curved cutter stapler in low colorectal anastomosis
bao-ren, YANG ; ji-wei, ZHANG ; ming, YANG ; long, CUI
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(10):-
Objective To evaluate the value of double stapling technique with curved cutter stapler in colorectal anastomosis,especially in low colorectal anastomosis. Methods The clinical data of 168 cases of rectal carcinomas treated with double stapling technique with curved cutter stapler were retrospectively reviewed.The intraoperative condition,postoperative complications and findings during follow up were analysed. Results During the operations,the processes of closure and anastomosis of all the patients were satisfactory,and no operative death occurred.After the operations,4 cases(2.4%) had anastomotic leakage,3 cases(1.8%) had anastomotic bleeding,and 2 cases(1.2%) had rectovaginal fistula.All the complications were cured.There was no anastomotic stricture. Conclusion Double stapling technique with curved cutter stapler may help to accomplish low colorectal anastomosis which is a difficult task for handed suture.
7.Perspective and application of metabonomics in modern study of traditional Chinese medicine.
Kun-Ming QIN ; Bin WANG ; Lin-Wei CHEN ; Mao-Sen ZHANG ; Guang-Ming YANG ; Ya-Chun SHU ; Bao-Chang CAI
China Journal of Chinese Materia Medica 2014;39(16):3010-3017
Metabonomics is a new method to study on the metabolic network and the relationship between body and environment, which conforms to the way of traditional Chinese medicine (TCM) research. In the study process of modernization of traditional Chinese medicine, effectively conjunction with metabonomics method will facilitate the integration of TCM with modern biological science and technology, and promote the modernization of TCM. This paper introduce the application of metabonomics in the research of toxicity mechanism of TCM, compatibility mechanism of TCM formula, pharmacology effect of TCM and processing mechanism of TCM. This paper summarize the problems in the TCM metabonomics research and prospect its bright future.
Animals
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Drug Therapy
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Drugs, Chinese Herbal
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adverse effects
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analysis
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metabolism
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therapeutic use
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Humans
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Medicine, Chinese Traditional
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methods
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trends
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Metabolomics
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methods
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trends
8.Influence of hyperbaric oxygen treatment on neural plasticity in experimental rats.
Ming-Ming MA ; Le ZHANG ; Bao-Qiong LIU
Journal of Central South University(Medical Sciences) 2008;33(12):1113-1118
OBJECTIVE:
To explore the influence of hyperbaric oxygen (HBO) treatment on neural plasticity and it's mechanism in experimental rats with cerebral ischemia.
METHODS:
Ninety-healthy male adult Sprague-Dawley rats (3 to approximately 4 month old) were randomly divided into a pseudo-operative group, a model group, and an HBO therapy group. The middle cerebral artery occlusion model was duplicated with suture methods, then we used beam walking test (BWT) to determine the motor skill of the rats and immunohistochemistry method to detect the distribution and location of microtubule-associated protein-2 (MAP-2) and glial fibrillary acidic protein (GFAP). Quantitative real-time PCR was used to detect the expression of Map-2 mRNA and GFAP mRNA.
RESULTS:
Immunohistochemistry showed that the fluorescence gray scale value of Map-2 in the HBO group was the highest in 3 groups at 1st week and 2nd week (P<0.05).The value of GFAP was lower than that of the model group but higher than that of the sham operated group (P<0.05). Real-time fluorescence-quantitative PCR indicated that the Map-2 mRNA of HBO group was the highest in 3 groups at 1st week and 2nd week (P<0.05); but the value of GFAP mRNA in the HBO group is lower than that of the model group,but higher than that of the sham operated group at 1st week and 2nd week (P<0.05).
CONCLUSION
After cerebral infarction, giving hyperbaric oxygenation treatment can improve the limbs motor function, and hyperbaric oxygenation treatment can increase the expression of Map-2 and decrease the expression of GFAP, which promote neural plasticity.
Animals
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Brain Infarction
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physiopathology
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therapy
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Glial Fibrillary Acidic Protein
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genetics
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metabolism
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Hyperbaric Oxygenation
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Infarction, Middle Cerebral Artery
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physiopathology
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therapy
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Male
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Microtubule-Associated Proteins
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genetics
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metabolism
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Neuronal Plasticity
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drug effects
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RNA, Messenger
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genetics
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metabolism
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Random Allocation
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Rats
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Rats, Sprague-Dawley
9.Effect of Tanreqing injection on immune activity of peripheral blood lymphocytes of patients with lung cancer.
Ming MA ; Xing-xiao YANG ; Jie ZHANG ; Lian-mei ZHAO ; Li-hua LIU ; Bao-en SHAN
China Journal of Chinese Materia Medica 2015;40(6):1207-1211
To investigate the effect of Tanreqing injection on immune activity of peripheral blood lymphocytes of patients with lung cancer. The peripheral blood lymphocytes of patients with lung cancer and healthy persons were separated by the density gradient centrifugation method for subsequent experiments, with those from healthy persons as the positive control. The effect of Tanreqing injection on stimulating the proliferation of lymphocytes with phytohemagglutinin (PHA) was determined by MTT method. The effect of Tanreqing injection on the lymphocyte secretions of IFN-γ and TNF-α and the subset ratio of lymphocytes cultured separately or with Tanreqing injection of different concentrations were examined by ELISA and flow cytometry (FCM) respectively. In addition, the LDH release assay was used to detect the cytotoxicity of cytotoxic T cells (CTL) and natural killer cells (NK). According to the findings, all of immunological indexes of lymphocytes from patients with lung cancer were weaker than that of healthy persons, but with the obvious increases in proliferation activity and IFN-γ and TNF-α secretions of lymphocytes co-cultured with Tanreqing Injection (P < 0.05). Among lymphocyte subsets co-cultured with Tanreqing Injection, CD3+, CD3+ CD4+ and CD3- CD16 + 56+ cell ratios notably increased, whereas CD4+ CD25+ Treg cell ratio obviously decreased (P < 0.05). In the meantime, Tanreqing injection can markedly promote the cytotoxicities of CTL and NK (P < 0.05). In conclusion, Tanreqing injection shows a significant effect in promoting the immune activity of lymphocytes from patients with lung cancer and their anti-tumor immunity.
Cell Proliferation
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drug effects
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Cells, Cultured
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Drugs, Chinese Herbal
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administration & dosage
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Humans
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Interferon-gamma
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genetics
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immunology
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Killer Cells, Natural
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drug effects
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immunology
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Lung Neoplasms
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drug therapy
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genetics
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immunology
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physiopathology
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T-Lymphocytes, Cytotoxic
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drug effects
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immunology
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Tumor Necrosis Factor-alpha
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genetics
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immunology
10.Study on RhBMP-2 induced osteoporosis rat BMSCs in vitro osteogenesis and VEGF expression.
Jun LI ; Yun WANG ; Xiao-ming BAO ; Peng-bin WEI ; Min ZHANG
China Journal of Orthopaedics and Traumatology 2015;28(5):446-449
OBJECTIVETo observe the impact of bone morphogenetic protein-2 (rhBMP-2) on bone marrow stromal cells (BMSCs) osteogenesis in vitro and vascular endothelial growth factors (VEGF) expression in bone osteoporotic to prevent and treat the osteoporosis.
METHODSTwenty 6-month-old female SD rats weighted (300±20) g underwent bilateral ovariectomized. At 3 months after operation, dual-energy X-ray absorptiometry was used to measure bone mineral density of rats,the values were compared with preoperative to ensure the model successfully, and the osteoporosis rats' BMSCs were cultured by bone marrow adherent cultured and the BMSCs morphology was observed under a phase contrast microscope upside down. The osteoporosis rats' BMSCs at the 2nd generation (p2) were randomly divided into experimental and control groups and were added complete medium (containing rhBMP-2) and osteogenic induced liquid, respectively. Two weeks later, the formation of cell calcium nodules were detected by Alizarin red staining method,alkaline phosphatase activity was measured by enzyme standard instrument and the expression of VEGF was detected by RT-PCT method.
RESULTS(1)Whole body bone mineral density of rats before and after operation were (0.179±0.007), (0.158±0.006) g/cm2,there was statistically significant (t=4.180,P< 0.05). (2)Alizarin red staining at 2 weeks after osteogenesis induced by BMSCs (P2) in the experimental group had more strong dyeing effect than the control group obviously. (3)Alkaline phosphatase activity at 2 weeks after osteogenesis induced by BMSCs (P2) of the experimental group (15.62±1.27) ug/gprot was significantly higher than that of the control group (8.62±0.93) ug/gprot,there was statistically significant (t=7.709, P<0.01). (4)The expression of VEGF at 2 weeks after osteogenesis induced by BMSCs (P2) of the experimental group 3.723±0.143 was significantly higher than that of the control group 0.950±0.072, there was statistically significant (t=29.462, P<0.01).
CONCLUSIONRhBMP-2 can improve the in-vitro osteogenesis ability of ovary osteoporosis rat BMSCs, promote the VEGF expression of osteogenesis factor. Regulating the VEGF expression may be one of the mechanisms of BMP-2 to participate in bone metabolism.
Animals ; Bone Density ; Bone Morphogenetic Protein 2 ; genetics ; metabolism ; Cells, Cultured ; Female ; Humans ; In Vitro Techniques ; Mesenchymal Stromal Cells ; cytology ; metabolism ; Osteogenesis ; Osteoporosis, Postmenopausal ; genetics ; metabolism ; physiopathology ; Rats ; Rats, Sprague-Dawley ; Vascular Endothelial Growth Factor A ; genetics ; metabolism