1.Advances of Genetic Transformation of Tall Fescue
Wei-Fei WANG ; Lie-Bao HAN ; Hui-Ming ZENG ;
China Biotechnology 2006;0(08):-
With the rapid development of turfgrass industry in our country,more and more attentions are paid to the genetic breeding of turfgrass. Biotechnology has great application potential in varietals improvement of tall fescue,which is commonly grown as cool-season turf grass in the temperate region. The establishment of tall fescue regeneration and genetic transformation systems was summarized. Meanwhile,the historical and present situations of tall fescue breeding are introduced. The part of tall fescue regeneration was expounded from different explants and approaches. While,the part of genetic transformations was expounded from different means. At last,problems and prospects of genetic transformation of tall fescue are discussed.
2.Damage to the brachial plexus produced by pulsed radiofrequency and radiofrequency thermocoagulation in rats
Wei-Yi GONG ; Yu-Ming PENG ; Bao-Guo WANG ;
Chinese Journal of Anesthesiology 1994;0(06):-
Objective To evaluate the damage to the brochial plexus produced by pulsed radiofrequency (PRF)and radiofrequency thermocoagulation(RFTC).Methods Fifty-five male Wistar rats weighing 250-300 g were randomly divided into 3 groups:groupⅠPRF(n=25):groupⅡRFTC(n=25)and groupⅢnormal control(n=5).The animals were anesthetized with intraperitoneal chloral hydrate.The left brochial plexus was exposed and PRF or RFTC was applied to the left brochial plexus.The voltage and current of the minimal stimulation which elecited muscle twitching and the impedance before and after operation were recorded in group PRF and RFTC.The nerve function was scored according to Tarloo(0=flaccid paresis,5=normal gait)before and at 3d after operation.The animals were killed and the left brachial plexus was removed immediately and at 1, 7,14,30 d after operation(n=5 at each time point)for determination of histopathological changes using microscope.Results The impedance and Tarlov score were significantly decreased after operation as compared to the baseline values before operation in group RFTC and were also significantly lower than in group PRF. Microscopic examination showed that the myelinated nerve fibers exhibited Wallerian degeneration and axon regeneration and the cytochondria in cylindraxile were severely injured or disappeared in group RFTC.The myelinated nerve fibers and the cytochondria in cylindraxile were significantly less injured after operation in group PRF than in group RFTC and returned to normal at 7 d and 30 d respectively.Conclusion The injury to brachial plexus produced by PRF is slighter than that produced by RFTC.
4.Determination of Phenoxyacetic Acid and 2,4-Dichlorophenoxyacetic Acid Using Solid-phase Extraction and High Performance Liquid Chromatography
Jinchang LI ; Lu WANG ; Mingyou HAN ; Ming BAO ; Guang WANG ; Yuanhong WANG
Chinese Journal of Analytical Chemistry 2001;29(5):580-582
A high performance liquid chromatographic method for the determination of phenoxyacetic acid and 2,4-dichlorophroxyacetic acid in environnmental waters was described.Phenoxyacetic acid and 2, 4-dichioropheroxyacetic acid in sample were enriched on Sep-Pak C18 column,and methanol was employed as elutent.The shim-pack CLC ODS (150mm×6.0mm i.d.) column was used as separation column at 40°C and eluted with methanol-water (9:1,V/V)(pH 3.0) at flow rate of 1.0mL/min,the detection wavelength was 275 nm.The recoveries of the two compounds were>98%.The detection limit was 0.04mg/L.
5.A fMRI study of item memory and source memory
Ming-Quan WANG ; Kai WANG ; Yong-Qiang YU ; Hai-Bao WANG ; Cheng ZHANG ; Jin-Min WU ;
Chinese Journal of Neurology 2000;0(05):-
Objective To examine the neural basis of item memory and source memory with fMRI approach.Methods Eight male and eight female healthy fight-handed native Chinese speakers were involved in this study.The item memory and source memory task were conducted with 504 highly frequent Chinese double-character words in the Block-designed experiment.Participants underwent such a double- round procedure as fMRI scanning following study.The fMRI data collected from a GE 1.5T MRI system were analyzed to generate corresponding activation maps for females and males respectively(P20)using statistical parametric mapping software(SPM).Results For females,item memory task activated the bilateral dorsolateral prefrontal cortex(BA6,the number of activated voxel clusters was 62 or 11 in the left and the right,respectively),source memory more activated the left dorsolateral prefrontal cortex(BA6/46,the number was 59).For males,item memory activated the right dorsolateral prefrontal cortex(BA6/46,the number was 64),source memory activated the bilateral dorsolateral prefrontal cortex(BA6,9 and 40 in the left and the right).Conclusion On the neural basis of item or source memory,there exists dissociation,which is that right dorsolateral prefrontal areas are more activated by item memory while left dorsolateral prefrontal areas by source memory.For the difference of gender,it is suggested that left dorsolateral prefrontal areas(BA6/46)are more activated in females while right dorsolateral prefrontal cortex(BA6/46)more in males.
6.Comparing different image registration methods in kilovolt cone-beam computed tomography image-guided radiotherapy for liver cancer
Jianting WANG ; Ming LIU ; Fushan ZHAI ; Anfeng WANG ; Xiaohui CAO ; Chaoen BAO
Chinese Journal of Radiation Oncology 2015;(4):444-448
Objective To study the methods of matching kilovolt CBCT image with planning CT scan. Methods A total of 121 CBCT scans were matched with planning CT scan using a manual and four automatic match methods by four observers in the offline. In the manual match, the live contour was used as a surrogate for image registration. Four automatic match methods, including routine soft?tissue match, routine bone match, automatic liver match and vertebral body match, were performed using image registration sofeware. First, the stability of the sofeware was tested. Then, the reproducibility of the same automatic match method was evaluated by comparing different observers’ match results. After the manual match by four observers, the mean of the match results was used as a standard to compare with others. The differerces was test by McNemar method. Results In the uniform match factors, automatic match result would not change. The reproducibility of routine soft?tissue and bone match are best, automatic vertebral body match is better than automatic liver match. Howerver, the automatic liver match result is the most similar to manual match, the percentages of match result have an absolute error no more than 3 mm in left?right, superior?inferior and anterior?posterior directions are 84?? 3%, 77?? 7% and 92?? 6%. Conclusions Automatic liver match can be used in image?guided radiotherapy for liver cancer, however, it should be performed by experienced oncologist and technologist together in each fraction, after that, the matching result should be adjusted carefully according to live contour.
7.Epidemiology and clinical analysis of serious corneal ulcer in 133 cases
Ming-Fang, XIA ; Ping-Bao, WANG ; Hua, WANG ; Xiao-Bo, XIA ; Qian, TAN
International Eye Science 2014;(9):1663-1666
To investigate and analyze the epidemiology of serious corneal ulcer.
● METHODS: A total of 133 serious corneal ulcercases from June 2010 to December 2013 in Xiangya Hospital Affiliated to Central South University were retrospectively reviewed for risk factors, demographic characteristics and diagnosis.
●RESULTS: There were 91 male patients (68. 4%) in the 133 patients. A peak incidence age was from 41 to 60 years old (82 cases, 44. 4%). Most of patients were peasant (77 cases, 57. 9%) and the percentage of the low schooling patients was 85. 0% (113 cases). The number of patients who had been sick was 73 before hospitalization (54. 9%) in all cases. The peak incidence season was January (34. 6%). Average length of stay was 9. 84d. The number of emergency cases was 44 (33. 1%). Most of patient were not only corneal ulcer cases but the hypopyon (92 cases, 69. 2%). The figure of inpatients who received operations was 102 cases ( 76. 7%). And the most of operations was amniotic membrane transplantation (31 cases, 39. 2%). Additionally, this data was classified regard to gender differences.
● CONCLUSlON: The peasant of the middle age and elderly represents a distinct clinical group in the serious corneal ulcer in hospital. Severity, ocular trauma in agriculture and health awareness are the major predisposing factors.
8.Chili arsenic contamination in southwest China and its influencing factors
Ming-guo, WANG ; She-hong, LI ; Bo, LI ; Jian-ming, ZHU ; Tang-fu, XIAO ; Bao-shan, ZHENG
Chinese Journal of Endemiology 2010;29(6):645-648
Objective To determine the distribution and influencing factors(dehydration method, storage time and chili varieties) of arsenic contents in chilies from southwest China, and the relationship between arsenic content and selenium content in chilies. Methods There were 272 dried chili samples, 76 groups of fresh chili samples and its corresponding soil samples, which were collected from the markets and peasant households in 76 counties of 9 regions in southwest China, and 36 dried chilies from other regions in China and abroad as a comparison. Their dehydration methods and storage time were investigated. The chilies were classified by Bailey Criteria. Arsenic content and selenium content in chilies were determined with hydride generation atomic fluorescence spectrometry. Arsenic content in soils were determined with water bath hydride generation atomic fluorescence spectrum. Results Their ranges of arsenic content in dried chili and fresh chili were 0.2 - 16 637.3,0.2 - 295.8 μg/kg, respectively. The median of arsenic content in the dried chili was 106.9 μg/kg while it was 0.2 μg/kg (dry weight) in the fresh chili. The chilies median arsenic of different drying methods and storage time, in order were: the furnace-dried stored for more 1 than year( 197.3 μg/kg), the sun-dried stored for more than 1 year (130.7 μg/kg), the furnace-dried stored for less than 1 year(94.1 μg/kg), the sun-dried stored for less than 1 year (55.5 μg/kg). The arsenic content of different kinds of solar-dried chilies and roast chilies were different. In solar-dried chilies, the median of arsenic contents from a order of high to low sequences were cluster chili (101.5 μg/kg), cherry chili (95.6 μg/kg), corn chili (86.8 μg/kg), and long chili (47. 1 μg/kg); in roast chilies, the median of arsenic contents from a order of high to low sequences were cherry chili(275.5 μg/kg), cluster chili (173.0 μg/kg), corn chili( 164.3 μg/kg), and long chili( 136.8 μg/kg). The medians of chilies from other regions of China and Turkey were higher than that of southwest China, their median were 125.8,112.3 μg/kg, respectively;the medians of chilies from America, France, and other countries were lower than that of southwest China, their median were 29.4,54.1,85.3 μg/kg, respectively. There was no significant correlation between fresh chilies and its corresponding soil arsenic(r = 0.010, P > 0.05). There was a significant positive correlation between the amount of arsenic and selenium in chilies(r = 0.616, P < 0.05). Conclusions The arsenic of dried chilies from southwest China was higher than that of fresh chilies. The arsenic of chili was different with different dehydration methods and storage time. There was a significant positive correlation between the amount of arsenic and selenium in chilies.
9.Perspective and application of metabonomics in modern study of traditional Chinese medicine.
Kun-Ming QIN ; Bin WANG ; Lin-Wei CHEN ; Mao-Sen ZHANG ; Guang-Ming YANG ; Ya-Chun SHU ; Bao-Chang CAI
China Journal of Chinese Materia Medica 2014;39(16):3010-3017
Metabonomics is a new method to study on the metabolic network and the relationship between body and environment, which conforms to the way of traditional Chinese medicine (TCM) research. In the study process of modernization of traditional Chinese medicine, effectively conjunction with metabonomics method will facilitate the integration of TCM with modern biological science and technology, and promote the modernization of TCM. This paper introduce the application of metabonomics in the research of toxicity mechanism of TCM, compatibility mechanism of TCM formula, pharmacology effect of TCM and processing mechanism of TCM. This paper summarize the problems in the TCM metabonomics research and prospect its bright future.
Animals
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Drug Therapy
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Drugs, Chinese Herbal
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adverse effects
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analysis
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metabolism
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therapeutic use
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Humans
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Medicine, Chinese Traditional
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methods
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trends
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Metabolomics
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methods
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trends
10.Emodin stimulates glucose uptake by HepG2 hepatocyte through activation of PPAR?
Li-Juan YANG ; Hai-Yan YU ; Yi-Ming MU ; Bao-An WANG ; Jing-Tao DOU ; Ju-Ming LU ; Chang-Yu PAN
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
Objective To construct PPAR?and PPAR?response element (PPRE)-controlled luciferase expression vectors,and to determine whether the traditional Chinese medicine emodin activates PPAR?and improves the glucose uptake by HepG2 hepatocytes.Methods (1) PPAR?and PPRE luciferase expression vectors were constructed and were applied to screen more than 20 ingredients of the traditional Chinese medicine. (2) HepG2 cells were incubated with emodin which can activate PPAR?and PPRE luciferase activity,and the PPAR?mRNA expression level was evaluated by RT-PCR/Southern blot.(3) PPAR?and glucose transporter 2 (Glut2) proteins were determined by Western blot analysis in HepG2 cells treated with emodin.(4) The glucose uptake rate was measured using 2-deoxy-[~3H]-D-glucose in HepG2 cells after treatment with emodin.Results (1) Emodin stimulated luciferase activity controlled by PPRE in dose-dependent manner at concentrations of 0.04 to 180?mol/L in COS-7 cells.The highest value was about 4 folds of control in the cells treated with 90?mol/L emodin (P