1.Value of cerebrospinal fluid leukocyte aggregation score in distinguishing the causes of meningitis.
Rui-mei HU ; Ruo-peng SUN ; Shu-hua GUO ; Bao-min LI ; Ji-wen WANG
Chinese Journal of Pediatrics 2003;41(6):463-465
Adolescent
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C-Reactive Protein
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metabolism
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Cell Aggregation
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Child
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Child, Preschool
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Cytodiagnosis
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methods
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Female
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Humans
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Infant
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Leukocytes
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cytology
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Male
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Meningitis, Bacterial
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blood
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cerebrospinal fluid
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diagnosis
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Meningitis, Viral
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blood
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cerebrospinal fluid
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diagnosis
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Sensitivity and Specificity
2.The relationship between initial 131Ⅰ uptake by lung metastases from differentiated thyroid cancer and treatment outcomes
Jing, CUI ; Hua-min, GE ; Bao-ping, LIU ; Guang-jun, NIU ; Xin-li, XIE ; Wei, CHANG
Chinese Journal of Nuclear Medicine 2011;31(4):230-232
Objective To investigate the potential relationship between initial 131Ⅰ uptake by lung metastases from differentiated thyroid cancer (DTC) and treatment outcomes. Methods From 1997 to 2009, 41 patients with DTC lung metastases were treated in the authors' department. 131Ⅰ whole body scan (WBS), serum Tg levels and other imaging results were analyzed to evaluate the therapeutic effects. Complete response (CR) or partial response (PR) was considered to be effective. The x2 test and correlation analysis were performed using SPSS 11.5 software package. Results 131 Ⅰ treatment was effective in 63% (26/41) patients with DTC lung metastases, CR in 8 patients and PR in 18 patients. In other 37% ( 15/41 ) patients, 131Ⅰ treatment was ineffective, including one case died of distant metastases. Patients with initial presence of 131Ⅰ lung uptake had higher effective rate than those with 131Ⅰ lung uptake during the second or later 131Ⅰ treatment (76% (22/29)vs33% (4/12),x2 =4.911, P=0.027). Also, significantly higher effective rate was found in patients with lung metastases alone than those with extra-pulmonary metastases (75% (24/32) vs 22% (2/9), x2 = 6. 312, P =0.012). However, the effective rate in patients with diffuse metastases was not significantly different from that in patients with focal metastases (67% (12/18)vs 61% ( 14/23), x2 =0. 146, P=0.702). The positive rate of initial 131Ⅰ uptake by lung metastases was higher in patients with total thyroidectomy than those with partial thyroidectomy (83% (24/29) vs 42% (5/12) ). Those positive rates in patients with papilary DTC and patients with follicular DTC were 72% (23/32) and 6/9, respectively. The surgical mode was correlated with the initial 131Ⅰ uptake by lung metastases (r = 0.411, P < 0.05), but no correlation was found between the histological type and the initial 131Ⅰ uptake by lung metastases ( r = 0. 047, P > 0.05 ). Conclusion Initial uptake of 131 Ⅰ by lung metastases alone is a favorable prognostic factor for DTC patients treated by131Ⅰ, and total thyroidectomy may be beneficial for initial 131Ⅰ uptake by lung metastases.
3.The molecular mechanism of interaction of trivalent dimethylarsinous acid (DMA(III)) binding to rat hemoglobin.
Min ZHANG ; Wenwen WANG ; Huifang JIN ; Lingling BAO ; Hua NARANMANDURA ; Yingjie QIN ; Chunhui LI
Acta Pharmaceutica Sinica 2014;49(5):666-71
In our previous work, we found that trivalent dimethylarsinous acid (DMA(III)) have high affinity binding to cysteine residue 13 of rat hemoglobin. However, it is still unknown why arsenic intermediate metabolite DMA(III) has high binding affinity for Cysl3 but not for other cysteine residues 93, 140, 111 and 125. In order to better understand the molecular mechanism of DMA(III) with rat hemoglobin, we have done current study. So, SD rats were divided into control and arsenic-treated groups randomly. Arsenic species in lysate of red blood cells were analyzed by HPLC-ICP-MS, and then determined by a hybrid quadrupole TOF MS. In addition, trivalent DMA(III) binds to different cysteine residues in rat hemoglobin alpha and beta chains were also simulated by Molecular Docking. Only Cys13 in alpha chain is able to bind to DMA(III) from the experiment results. Cys13 of alpha chain in rat hemoglobin is a specific binding site for DMA(III), and we found that amino acids compose pockets structure and surround Cys13 (but not other cysteine residues), make DMA(III) much easy to bind cysteine 13. Taken together, the DMA(III) specific binding to Cys13 is related to spatial structure of Cys13.
4.The protective effect of Gabexate mesilate on D-galactosamine-lipopolysaccharide-induced acute liver failure in rats
Bao LIU ; Hua WANG ; Min SHAO ; Ai'Jun PAN ; Linlin ZHANG ; Shusheng ZHOU ; Lei ZHANG ; Kui JIN ;
Chinese Journal of Emergency Medicine 2006;0(07):-
Objective To investigate the protective effect of Gabexate mesilate(GM)on D-galactosamine- lipopolysaccharide-indneed acute liver failure in rats.Methods The model of acute liver failure in rats was produced by injection of D-galactosamine(D-GalN)and lipopolysaccharide(LPS).The alanine aminotransferase(ALT),aspartate aminotransferase(AST)in serum and malondiadehyde(MDA)content,superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)activities in liver homogenate were assayed by spectrophotometry.The levels of turnout necrosis factor-?(TNF-?),interleukin-?(IL-?)and interleukin-6(IL-6)were determined by ELISA method.Hepatic pathological examination was observed.Results 25 mg?kg~(-1),50mg?kg~(1),100 mg?kg~(-1) of GM significantly decreased the serum transaminase activities,the infiltration of inflammatory cells,and MDA content,hut didn't reduce SOD and GSH- PX activities in liver homogenate.GM significantly reduced TNF-?,IL-1?and IL-6 levels in serum.Conclusions GM showed significant protective effects on acute liver failure in rats.
5.Screening of HIV-1 neutralizing antibody mimotopes in slow progressor from phage display peptide library
Xiaoli ZHANG ; Xiaoxu HAN ; Di DAI ; Mingjia BAO ; Zining ZHANG ; Min ZHAO ; Hua NIAN ; Hong SHANG
Chinese Journal of Laboratory Medicine 2012;35(9):838-842
ObjectiveTo screen mimetic HIV-1 neutralizing epitopes from plasma with high level neutralizing antibody,and to provide useful information for further study of the interaction between antigen and antibody.MethodsIn order to gain neutralizing antibody recognized mimotopes, we detected neutralizing antibodieslevelsof 11HIV-1infectedslowprogressorsbyPBMC-basedneutralization assays.High-titer HIV-neutralizing antibodies from plasma of SPs was used as the ligand for biopanning by phage-displayed random peptide library.Positive phage clones was evaluated by ELISA,sequenced,and analyzed for homology to HIV-1 env by local BLAST to deduce the neutralizing peptide.ResultsTwenty-two clones were obtained consistent with requirement through three rounds biopanning.After comparison analysis,twelve clones include C8 were obtained as mimotopes of neutralizing antibody,C40 located in gp41Ⅱ cluster with the highest titer by inhibition ratio may be as neutralizing epitope.Conclusion By the use of IgG antibodies from SPs to screen the phage random polypeptide library,one can acquire multiple phage mimetic peptides of HIV related antigen epitope.(Chin J Lab Med,2012,35:838-842 )
6.Screening of benzodizepines and their metabolites in urine.
Bao-hua SHEN ; Min SHEN ; Xian-yi ZHUO
Journal of Forensic Medicine 2002;18(1):22-25
OBJECTIVE:
Screen seventeen benzodizepines and their metabolitesin urine by GC/ECD and GC/MS.
METHODS:
They were GC (GC/ECD, GC/MS) assay of benzodizepines and GC (GC/ECD, GC/MS) assay of benzophenones of acid-hydrolytic products of 1,4-benzodizepines.
RESULTS:
The methods were simple and sensitive. The recoverys were 60% to 90% of most benzodizepines, linear calibration curves were 20 ng/ml-200 ng/ml (r > 0.99), and detection limits were 0.5 ng/ml-10 ng/ml.
CONCLUSION
They methods were evaluated with human urine samples.
Anti-Anxiety Agents/urine*
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Benzodiazepines/urine*
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Benzophenones/urine*
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Chromatography, Gas
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Gas Chromatography-Mass Spectrometry
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Humans
9.The molecular mechanism of interaction of trivalent dimethylarsinous acid (DMA(III)) binding to rat hemoglobin.
Min ZHANG ; Wen-Wen WANG ; Hui-Fang JIN ; Ling-Ling BAO ; Hua NARANMANDURA ; Ying-Jie QIN ; Chun-Hui LI
Acta Pharmaceutica Sinica 2014;49(5):666-671
In our previous work, we found that trivalent dimethylarsinous acid (DMA(III)) have high affinity binding to cysteine residue 13 of rat hemoglobin. However, it is still unknown why arsenic intermediate metabolite DMA(III) has high binding affinity for Cysl3 but not for other cysteine residues 93, 140, 111 and 125. In order to better understand the molecular mechanism of DMA(III) with rat hemoglobin, we have done current study. So, SD rats were divided into control and arsenic-treated groups randomly. Arsenic species in lysate of red blood cells were analyzed by HPLC-ICP-MS, and then determined by a hybrid quadrupole TOF MS. In addition, trivalent DMA(III) binds to different cysteine residues in rat hemoglobin alpha and beta chains were also simulated by Molecular Docking. Only Cys13 in alpha chain is able to bind to DMA(III) from the experiment results. Cys13 of alpha chain in rat hemoglobin is a specific binding site for DMA(III), and we found that amino acids compose pockets structure and surround Cys13 (but not other cysteine residues), make DMA(III) much easy to bind cysteine 13. Taken together, the DMA(III) specific binding to Cys13 is related to spatial structure of Cys13.
Animals
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Arsenic
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metabolism
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Binding Sites
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Cacodylic Acid
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analogs & derivatives
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chemistry
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Chromatography, High Pressure Liquid
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Cysteine
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metabolism
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Hemoglobins
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metabolism
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Mass Spectrometry
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Peptide Fragments
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metabolism
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Rats
10.The 3D PACS image system based on pipeline framework.
Hui ZHANG ; Xu-dong BAO ; Song-yi LI ; Hua-zhong SHU ; Li-min LUO
Chinese Journal of Medical Instrumentation 2002;26(2):84-87
We put forward an image rendering system based on pipeline framework for processing and displaying medical images. Compared to original computer graphics algorithms divided into volume rendering and surface rendering, this framework can effectively comprehend methods of computer graphics and image processing, import some new concepts such as vertex buffer, pixel buffer and texture buffer. We implement Shaded Surface Display, Maximum Intensity Projection, Digitally Reconstructed Radiography, Multi planar Reformation, Curved Planar Reformation and Interactive Virtual Endoscopy in our new developed PACS image system.
Algorithms
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Computer Systems
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Image Processing, Computer-Assisted
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methods
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Imaging, Three-Dimensional
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Medical Records Systems, Computerized
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Radiology Information Systems
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Software