1.The Effect of Combination Use of 1,25(OH)_2D_3 and Celecoxib on Growth and Apoptosis in Breast Cancer Cell Line Hs578T
Xueqi HE ; Yangyi BAO ; Xin SUN ; Gang MENG ; Qin WANG
Journal of Medical Research 2006;0(08):-
Objective To study the effect of combined 1,25-dihydroxyvitamin D31,25(OH)2D3 and celecoxib on growth, call cycle and apoptosis in breast cancer cell line Hs578T.Methods We compared cell numbers by using MTT method , analyzed cell cycle percentage and apoptosis with flow cytometric.Results Both with 1,25(OH)2D3 and celecoxib could inhibite tumov growth,induc apoptosis in a dose-time-dependent manner. comparing with 1,25(OH)2D3 alone,combination wse of the two drugs had a additive effect but was inferior to cececoxib alone. The combination use of 10-8mol/L1,25(OH)2D3 and celecoxib group is more effective than the combination use of 10-7mol/L1,25(OH)2D3 and celecoxib group. Conclusion 1,25(OH)2D3 and celecoxib could be a new drug for the prevention and treatment of breast cancer.
2.Cancer stem cells:current status
Zhi-Gang SUN ; Sheng-Dong HUANG ; Bao-Ren ZHANG ;
Academic Journal of Second Military Medical University 1981;0(04):-
Recently,study on cencer stem cells has been a focus of study.Cancer stem cell is a small population of cencer cells possessing the properties of stem cells:self-renewal,differentiation and proliferation.To date,the existence of cancer stem cells has been proven in acute and chronic myeloid leukemia,breast cancer,brain tumors,liver cancer and colon cancer,etc..In this article we reviews the current progress on cancer stem cells,including the defination,existing evidence,research methods, and challenges in clinical application.
3.Mechanisms of cordycepin on improving renal interstitial fibrosis via regulating eIF2α/TGF-β/Smad signaling pathway.
Liu-bao GU ; Rong-wen BIAN ; Yue TU ; Hao HU ; Yi-gang WAN ; Wei SUN
China Journal of Chinese Materia Medica 2014;39(21):4096-4101
OBJECTIVETo investigate the effects and mechanisms of cordycepin,an effective component of cordyceps militaris, on renal interstitial fibrosis (RIF) and its related eIF2α/TGF-β/Smad signaling pathway.
METHODFirstly, 15 C57BL/6 mice were randomly divided into 3 groups,the control group (Group A), the model group (Group B) and the cordycepin-treated group (Group C). After renal interstitial fibrotic model was successfully established by unilateral ureteral obstruction (UUO), the mice in Group C were intraperitoneally administrated with cordycepin(5 mg x kg(-1) d(-1)) and the ones in Group A and B were administrated with physiological saline for 5 days. At the end of the study, the obstructed kidneys were collected and detected for the pathological changes of RIF, and the mRNA expressions of collagen type I (Col I) and α-smooth muscle actin (α-SMA) in the kidney by Northern blot. Secondly, after renal tubular epithelial (NRK-52E) cells cultured in vitro were exposed to transforming growth factor (TGF) -β with or without cordycepin, the mRNA expressions of Col I and collagen type IV( Col IV) by Northern blot, and the protein expressions of eukaryotic initiation factor 2α (eIF2α), phosphorylated eIF2α ( p-eIF2α), Smad2/3 and phosphorylated Smad2/3 (p-Smad2/3) were tested by Western blot.
RESULTIn vivo, cordycepin alleviated RIF in model mice, including improving fibrotic pathological characteristics and mRNA expressions of Col I and α-SMA. In vitro, cordycepin induced the high expression of p-elF2α, and inhibited the expressions of p-Smad2/3, Col I and Col IV induced by TGF-β in NRK-52E cells.
CONCLUSIONCordycepin attenuates RIF in vivo and in vitro, probably by inducing the phosphorylation of eIF2α, suppressing the expression of p-Smad2/3, a key signaling molecule in TGF-β/Smad signaling pathway, and reducing the expressions of collagens and α-SMA in the kidney.
Actins ; analysis ; Animals ; Deoxyadenosines ; pharmacology ; Fibrosis ; Kidney ; drug effects ; pathology ; Male ; Mice ; Mice, Inbred C57BL ; Phosphorylation ; Protein-Serine-Threonine Kinases ; physiology ; Signal Transduction ; drug effects ; Smad Proteins ; physiology ; Transforming Growth Factor beta ; antagonists & inhibitors ; physiology
4.Molecular mechanism of rhein on inhibiting autophagic protein expression in renal tubular epithelial cells via regulating mTOR signaling pathway activation.
Yue TU ; Wei SUN ; Liu-bao GU ; Yi-Gang WAN ; Hao HU ; Hong LIU
China Journal of Chinese Materia Medica 2014;39(21):4090-4095
OBJECTIVETo explore the effects and molecular mechanisms of rhein on reducing starvation-induced autophagic protein expression in renal tubular epithelial ( NRK-52E) cells.
METHODHank's balanced salt solution (HBSS) was used to induce NRK-52E cells to be in the state of starvation. After the intervention of HBSS for 0, 0.5,1, 2 and 6 hours, firstly, the protein expression of microtubule-associated protein 1 light chain 3(LC3 I/II), which is a key protein in autophagy, was detected. Secondly, the protein expressions of mammalian target of rapamycin (mTOR) and phosphorylated-mTOR Ser2448 (p-mTOR S2448) were examined. And then, after the co-treatment of rhein (5 mg x L(-1)) and HBSS (1 mL) without or with mTOR inhibitor, rapamycin (100 nmol x L(-1)), the protein expressions of LC3 I/II, mTOR and p-mTOR S2448 were tested, respectively.
RESULTHBSS could induce the up-regulation of LC3 II and the down-regulation of p-mTOR S2448 at protein expression level in NRK-52E cells. The co-treatment of rhein and HBSS could reversely regulate the protein expressions of LC3 II and p-mTOR S2448 in NRK-52E cells significantly. The co-treatment of rapamycin, rhein and HBSS could recover the level of LC3 II protein expression in HBSS-intervened NRK-52E cells.
CONCLUSIONHBSS induces autophagy in renal tubular epithelial cells by inhibiting mTOR signaling pathway activation. Rhein reduces the autophagic protein expression in renal tubular epithelial cells through regulating mTOR signaling pathway activation, which is the possible effects and molecular mechanisms.
Animals ; Anthraquinones ; pharmacology ; Autophagy ; drug effects ; Cells, Cultured ; Epithelial Cells ; drug effects ; metabolism ; Isotonic Solutions ; pharmacology ; Kidney Tubules ; drug effects ; metabolism ; Microtubule-Associated Proteins ; genetics ; Rats ; Signal Transduction ; drug effects ; TOR Serine-Threonine Kinases ; antagonists & inhibitors ; genetics ; physiology
5.The diagnosis and treatment of rotational unstable nonunion of femoral shaft fracture after interlocking nailing.
Gang ZHAO ; Bo-song ZHANG ; Lin SUN ; Xin-bao WU
Chinese Journal of Surgery 2009;47(16):1232-1235
OBJECTIVETo evaluate the principle and treatment of rotational unstable nonunion of the femoral shaft fracture after interlocking nailing.
METHODSFrom April 2003 to June 2007, 18 cases of rotational unstable nonunion of femoral shaft fractures after interlocking nailing were treated with exchanged reamed nailing and/or LCP fixation and iliac bone graft. The average age was 40 years old (from 22 to 52).
RESULTSAll patients were followed up for an average time of 28 months (from 12 to 58). All the fractures were united in an average time of 7 months (from 6 to 8). There were no perioperative complications occurred and no wound infection were observed. No loosening was found around the implant till the last follow-up.
CONCLUSIONSRotational instability is one of the reasons of nonunion of femoral shaft fracture after interlocking nailing. Reaming and nail exchanging or LCP fixation with iliac bone graft are the appropriate methods which can achieve a great clinic results.
Adult ; Female ; Femoral Fractures ; surgery ; Follow-Up Studies ; Fracture Fixation, Intramedullary ; methods ; Fractures, Ununited ; surgery ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Young Adult
7.Blood lactic acid level and APACHE II score on prognosis of critically ill elderly patients
Bin BAO ; Zhi-Gang LI ; Xiao-Lin SUN
Chinese Journal of Epidemiology 2012;33(4):428-430
Objective To analyze the relevance between blood lactic acid level and acutephysiology and chronic health evaluation Ⅱ (APACHE Ⅱ ) score in order to provide guideline for clinical treatment.Methods Retrospective analyses on 537 critically ill elderly patients who were hospitalized in the ICU with their blood lactic acid level tested and APACHE Ⅱ scores calculated.Results The overall death rate was 35.75% (192/537) with the APACHE Ⅱ score as (22.6 ± 12.8),and blood lactic acid level as (6.84 ± 2.01 ) mmol/L.The blood lactic acid level among deaths was obviously higher than in the control group,with significant differences (P<0.05).The level of blood lactic acid was positively related to APACHE Ⅱ score (r=0.572,P<0.05) while the death rate was both positively related to APACHE Ⅱ score (r=0.475,P<0.05) and the level of blood lactic acid (r=0.506,P<0.05).Conclusion There seemed a positive correlation between blood lactic acid level and the APACHE Ⅱscore.Both of them showed good relevancc with thc prognosis of the disease.
8.Molecular biology advance in the Rh blood group system.
Zhi-gang SUN ; Mei DING ; Bao-jie WANG
Journal of Forensic Medicine 2005;21(1):65-67
The Rh blood group system is one of the most complex of the known human blood group polymorphisms, including above 45 blood group antigens. Considerable progress has been made in our understanding of the molecular basis of Rh in the past 10 years. The bases of Rh inheritance, RH gene and its evolution, the structure and function of Rh complex, as well as nonerythroid Rh homolog, have been determined. Further improvements have been made in the technology of Rh genotyping. The review provides an update on the advance of Rh blood group to give information in the practice of forensic science.
Blood Group Antigens/genetics*
;
Blood Proteins/immunology*
;
DNA Fingerprinting
;
Forensic Medicine/methods*
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Genotype
;
Humans
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Molecular Biology
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Mutation
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Polymorphism, Genetic
;
Rh-Hr Blood-Group System/genetics*
9.RHCE genotyping in Chinese Han from north and Li from south.
Zhi-Gang SUN ; Mei DING ; Bao-Jie WANG ; Hong-Wu HUANG
Journal of Forensic Medicine 2006;22(4):271-274
OBJECTIVE:
To establish a method which processes RHCE genotyping with PCR.
METHODS:
Using PCR-SSP to detect RHCE genotype in 200 cases of Han population in north of China and Li population in south of China and detecting 5 samples of parentage testing at the same time.
RESULTS:
The results of RHCE genotyping in individuals of two populations are completely accorded with the results of serology typing. And the distribution of RH genotypic frequency in Han population in north China is: RHCCEE 1, RHCCEe 3, RHCCee 88, RHCcEE 4, RHCcEe 20, RHCcee 54, RHccEE 1, RHccEe 22, RHccee; The distribution of RH genotypic frequency in Li populatin in south China is RHCCEE 2, RHCCEe 2, RHCCee 106, RHCcEE 7, RHCcEe 62, RHCcee 10, RHccEE 3, RHccEe 8. The results of RHCE genotype detecting of parentage testing samples are accorded with the results of associated identification of 13 STR loci.
CONCLUSION
PCR-SSP technology can exactly detect RHCE genotype in individuals of Han population in north China and Li population in south China.
Alleles
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Asian People/genetics*
;
China/ethnology*
;
DNA/blood*
;
DNA Primers
;
Gene Frequency
;
Genetics, Population
;
Genotype
;
Humans
;
Polymerase Chain Reaction/methods*
;
Rh-Hr Blood-Group System/genetics*
10.Identification of Max binding protein as a novel binding protein of Nck1 and characterization of its role in inhibiting human liver cancer SK-HEP-1 cells.
Qi ZHOU ; Tao HUANG ; Ya-feng WANG ; Kun-sun ZHANG ; Dong CHEN ; Bao-gang PENG
Chinese Medical Journal 2012;125(18):3336-3339
BACKGROUNDThe tendency of tumor cells to disperse throughout the liver is a distinct feature of hepatocellular carcinoma (HCC). Nck family adaptor proteins function to regulate actin cytoskeletal reorganization that leads to cell motility. We previously found that Max binding protein (MNT) was differentially expressed in HCC, and interacted with Nck1 by 2-DE. MNT is a protein member of the Myc/Max/Mad network which plays roles in cell proliferation, differentiation, and death. We investigated the effects of MNT on migration of human liver cancer SK-HEP-1 cells to study the migration regulatory role of MNT in HCC cells.
METHODSInteraction between MNT and Nck1 was further validated in hepatoma cells by GST-pull down assay and immunoprecipitation. siRNAs specific to MNT (MNT siRNA) were used to knockdown MNT expression. Western blotting, transwell assay were used to determine the migration potential of cells.
RESULTSInteraction between MNT and Nck1 was validated in hepatoma cells. MNT knockdown promoted the migration of human liver cancer SK-HEP-1 cells (P < 0.01).
CONCLUSIONThe results suggest that MNT, via interaction with Nck1, inhibits hepatoma cell migration.
Adaptor Proteins, Signal Transducing ; genetics ; metabolism ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ; genetics ; metabolism ; Blotting, Western ; Cell Differentiation ; genetics ; physiology ; Cell Line, Tumor ; Cell Movement ; genetics ; physiology ; Humans ; Immunoprecipitation ; Liver Neoplasms ; Oncogene Proteins ; genetics ; metabolism ; Protein Binding ; genetics ; physiology ; Repressor Proteins ; genetics ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction