1.Advances of vasculogenic mimicry in tumors.
Chinese Journal of Pathology 2007;36(10):694-697
Angiogenesis Inhibitors
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pharmacology
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Angiostatins
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pharmacology
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Animals
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Antigens, CD
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metabolism
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Cadherins
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metabolism
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Humans
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Matrix Metalloproteinase Inhibitors
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Matrix Metalloproteinases
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metabolism
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Microvessels
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metabolism
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Neoplasms
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blood supply
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pathology
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Neovascularization, Pathologic
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metabolism
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pathology
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Vascular Endothelial Growth Factor A
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metabolism
2.Protective effect of arctigenin in GK rats combined with hypertension macroangiopathy.
Qin FENG ; Bao-cun SUN ; Wen-kai XIA
China Journal of Chinese Materia Medica 2015;40(5):957-962
To study the protective effect of Arctigenin in goto-kakizaki (GK) rats combined with hypertension macroangiopathy. Six-week-old GK rats were divided randomly according to blood glucose level into four groups: the model group and low, middle and high dose arctigenin groups (12.5, 25, 50 mg x kg(-1)), with Wistar rats as the normal group. All of GK rats were given high-glucose and high-fat diet. After 16 weeks, GK rats were orally administrated with 10 mg x kg(-1) x d(-1) N-Ω-nitro-L-arginine methyl ester for eight weeks. During the modeling, all of arctigenin groups were orally administrated with different dose of arctigenin twice a day; The model group and the normal group were given solvents. At the beginning, mid-term and end of the experiment, blood glucose was measured. At the end of the experiment, efforts were made to detect blood pressure, collect abdominal aortic blood after anesthesia, fix thoracic aorta after bloodletting to make paraffin sections, observe morphological characteristics and detect the expression of VEGF by immunohistochemistry. According to the results, the blood glucose rose in all GK rats, with no significant difference between the drug group and the model group. At the end of the experiment, the blood pressure significantly increased in GK rats, indicating that Arctigenin could notably reduce the blood pressure in GK rats in a dose-dependent manner. The blood routine test showed increases in both the total white blood cell count and differential blood count, MPV and PDW, abnormal blood platelet parameters and decrease in PLT in GK rats, suggesting that Arctigenin could remarkably reduce the total white blood cell count and differential blood count, MPV and PDW. The thoracic aortic morphological observation revealed obvious endangium lesions in GK rats, demonstrating that Arctigenin could ameliorate the lesion extent. VEGF immumohistochemical staining showed a higher VEGF expression in the model group but lower expression in Arctigenin groups. In conclusion, Arctigenin had a protective effect on aorta in GK rats. Its mechanism may be related to blood pressure lowering, anti-inflammation, improvement in blood platelet function and reduction of VEGF expression.
Animals
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Blood Glucose
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metabolism
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Blood Pressure
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drug effects
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Diabetes Mellitus, Type 2
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complications
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metabolism
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physiopathology
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Diabetic Angiopathies
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etiology
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metabolism
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physiopathology
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prevention & control
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Disease Models, Animal
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Drugs, Chinese Herbal
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administration & dosage
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Furans
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administration & dosage
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Humans
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Hypertension
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etiology
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metabolism
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physiopathology
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prevention & control
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Lignans
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administration & dosage
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Male
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Rats
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Rats, Wistar
5.Correlation between the expression of collgen IV, VEGF and vasculogenic mimicry.
Xi-shan HAO ; Bao-cun SUN ; Shi-wu ZHANG ; Xiu-lan ZHAO
Chinese Journal of Oncology 2003;25(6):524-526
OBJECTIVETo study the relationship between collgen (Col) IV, VEGF secreted by the tumor cells and vasculogenic mimicry (VM).
METHODS158 bi-phase differential malignant tumor specimens were alloted and made into tissue microarray. These tissue microarray sections were stained with CD31, periodic acid-Schiff (PAS) and Col IV. Subsequently, distributive trait of Col IV and the difference of VEGF expression were analyzed.
RESULTSThe basement membrane of VM was PAS and Col IV positive. The expression of VEGF in bi-phase differential malignant tumor with VM was less than that in those without VM. The difference of VEGF expression in malignant melanoma and alveolar rhabdomyosarcoma was significant (P < 0.05).
CONCLUSIONCollgen IV and periodic acid-Schiff positive material take part in constructing the basement membrane of vasculogenic mimicry. The difference of the VEGF expression proves that vasculogenic mimicry can sustain the tumor blood supply.
Collagen Type IV ; analysis ; Humans ; Immunohistochemistry ; Neoplasms ; blood supply ; chemistry ; pathology ; Periodic Acid-Schiff Reaction ; Vascular Endothelial Growth Factor A ; analysis
6.Pilot study of differentiation of bone marrow derived mesenchymal stem cells into endothelial cells induced by B16 melanoma cells in vitro.
Chun-Sheng NI ; Nan ZHAO ; Tao SUN ; Xiu-Lan ZHAO ; Xing-Hui WANG ; Qiang GU ; Bao-Cun SUN
Chinese Journal of Pathology 2009;38(6):402-407
OBJECTIVEBone-marrow derived mesenchymal stem cells (BMSC) have the potential to differentiate into endothelial cells. The aim of the study was to investigate the induction process of BMSC by B16 melanoma cells in vitro and to analyze the role of VEGF-a in the process.
METHODSA co-culture system containing BMSC and B16 melanoma cells based on transwell indirect model was established, and the induction process of BMSC by B16 melanoma cells was studied in vitro.
RESULTSBMSC were isolated from the bone marrow of C57 mice. BMSC expressed CD105, CD90, CD73, CD44 and CD166, and acquired expressin of endothelial phenotype markers including VEGFR-1, VEGFR-2 and Factor VIII after co-culture with B16 melanoma cells for 48 hours. The expression level of VEGFR-2 would be double and Factor VIII threefold more by extending the co-culture time to 72 hours. In the co-culture system, B16 melanoma cells also up-regulated the expression of VEGF-a.
CONCLUSIONSVEGF-a plays a significant role in the differentiation of BMSC into cells of endothelial phenotype, therefore, is important to tumor angiogenesis.
Animals ; Bone Marrow Cells ; cytology ; metabolism ; Cell Differentiation ; Cell Line, Tumor ; Coculture Techniques ; Endothelial Cells ; cytology ; metabolism ; Factor VIII ; metabolism ; Male ; Melanoma, Experimental ; metabolism ; pathology ; Mesenchymal Stromal Cells ; cytology ; metabolism ; Mice ; Mice, Inbred C57BL ; Pilot Projects ; Vascular Endothelial Growth Factor A ; metabolism ; Vascular Endothelial Growth Factor Receptor-1 ; metabolism ; Vascular Endothelial Growth Factor Receptor-2 ; metabolism
7.Correlation between matrix metalloproteinases-2 and tissue inhibitor of metalloproteinase-2 expression, metastatic potential and tumor angiogenesis in synovial sarcoma and its prognostic significance.
Bao-cun SUN ; Yan SUN ; Xiu-lan ZHAO ; Jian WANG ; Xin WANG ; Yan-xue LIU
Chinese Journal of Pathology 2006;35(3):155-158
OBJECTIVETo study the expression of matrix metalloproteinases (MMP)-2 and tissue inhibitor of metalloproteinase (TIMP)-2 in tumor cells of synovial sarcoma and its clinical significance.
METHODSExpression of MMP-2 and TIMP-2 in tumor cells of 72 cases of synovial sarcoma was studied by immunohistochemistry. The profile was correlated with clinicopathologic parameters, microvessel density (MVD) (analyzed by CD31 immunostaining) and survival rate.
RESULTS(1) There was a statistically significant negative correlation between expression of MMP-2 and TIMP-2 (r = -0.290 and P = 0.013). (2) The proportion of high MMP-2 expression to low TIMP-2 expression in patients with tumor metastasis was significantly higher than that in patients without metastasis (P = 0.010 and 0.002 respectively). (3) MVD of patients with high MMP-2 expression was higher than that in the low MMP-2 expression group (P = 0.005). MVD of patients with high TIMP-2 expression was lower than that in the low TIMP-2 expression group (P = 0.048). (4) Low TIMP-2 expression significantly correlated with poor prognosis of patients with synovial sarcoma, by univariate and multivariate survival analysis (P = 0.002 and 0.016 respectively).
CONCLUSIONSExpression of MMP-2 and TIMP-2 correlates with metastatic potential and tumor angiogenesis in synovial sarcoma. Low TIMP-2 expression often indicates poor prognosis and unfavorable clinical outcomes.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Female ; Follow-Up Studies ; Humans ; Immunohistochemistry ; statistics & numerical data ; Kaplan-Meier Estimate ; Male ; Matrix Metalloproteinase 2 ; metabolism ; Microcirculation ; Middle Aged ; Neoplasm Metastasis ; Neoplasm Staging ; Neovascularization, Pathologic ; enzymology ; pathology ; Prognosis ; Proportional Hazards Models ; Sarcoma, Synovial ; blood supply ; enzymology ; pathology ; Tissue Inhibitor of Metalloproteinase-2 ; metabolism
8.Diagnostic value of SYT-SSX fusion gene detection by fluorescence in-situ hybridization for synovial sarcoma.
Yan SUN ; Bao-Cun SUN ; Yan-Xue LIU ; Shi-Wu ZHANG ; Xiu-Lan ZHAO ; Jian WANG ; Xi-Shan HAO
Chinese Journal of Pathology 2008;37(10):660-664
OBJECTIVETo establish a method of SYT-SSX fusion gene detection by FISH and to explore its diagnostic value for synovial sarcoma.
METHODSThe presence of SYT-SSX fusion gene was determined by FISH using a tissue microarray containing 62 known synovial sarcomas, 60 non-synovial sarcomas and 133 equivocal synovial sarcomas. FISH results were compared with those of RT-PCR published previously.
RESULTSOverall, 96.9% (247/255) of the cases were successfully analyzed by FISH. The sensitivity of FISH for known synovial sarcomas was 96.7% (58/60), and the specificity for the non-synovial sarcoma was 100% (59/59). Moreover, SYT-SSX gene fusion was detected in 78.1% (100/128) of the equivocal synovial sarcomas. The concordance rate between FISH and RT-PCR was 83.6% (102/122) in those equivocal synovial sarcomas, and overall 79.7% (106/133) of these cases were confirmed as synovial sarcomas either by RT-PCR or by FISH.
CONCLUSIONSThe sensitivity and specificity of FISH detection of SYT-SSX fusion gene are high. FISH and RT-PCR are complementary to each other in the confirmation of synovial sarcomas, particularly those questionable cases.
Biomarkers, Tumor ; analysis ; genetics ; Humans ; In Situ Hybridization, Fluorescence ; methods ; Nucleic Acid Hybridization ; methods ; Oncogene Proteins, Fusion ; genetics ; isolation & purification ; Pathology, Molecular ; methods ; Reverse Transcriptase Polymerase Chain Reaction ; Sarcoma, Synovial ; diagnosis ; genetics ; Soft Tissue Neoplasms ; diagnosis ; genetics
9.Relationship between the expression of hypoxia-inducible factor-1α and epidermal growth factor receptor and micro vessel density and their clinical significance
Peng LIN ; Wei WANG ; Wen-Juan CAI ; Chun-Rong HAN ; Yan SUN ; Man LI ; Bao-Cun SUN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2009;44(6):480-485
Objective Analyze the relationship between the expression of hypoxia-inducible factor-1α( HIF-1α)and epidermal growth factor receptor(EGFR) and CD105 micro vessel density (MVD) and their value in evaluating biologic behavior and prognosis in laryngeal cancer. Methods Ninty-one cases of laryngeal cancer were analyzed about their clinical and pathology data. In tumor tissue the expression of HIF-1α and EGFR was detected by immunohistochemistry and MVD was marked by CD105. Results The expression of HIF-1α was correlated with size, TNM stage, T stage, lymph node metastasis and histological grade( all P < 0. 05). The expression of EGFR was correlated with TNM stage, lymph node metastasis, histological grade and relapse(all P <0. 05). MVD was correlated with type, TNM stage, T stage, lymph node metastasis, metastasis and histological grade ( all P < 0. 05 ). The expression of HIF-1α and EGFR was correlated with MVD ( F value was 7. 644 and 5. 197 respectively, P value was 0. 001and 0. 025 respectively). The correlation between the expression of HIF-1α and EGFR was significant statistically( r = 0.238,P= 0.007). The survival rate of patients of 3 years and 5 years were 56.1% and 44.2% respectively. Survival analysis by Log Rank showed that prognosis of laryngeal cancer patients was correlated with type, TNM stage and the expression of both HIF-1αand EGFR. While Cox multiple factors analysis demonstrated that TNM stage and expression of EGFR were independent prognostic factor of laryngeal cancer. (P value was 0. 049 and 0. 041 respectively,RR was 1. 300 and 2. 417 respectively). Conclusions HIF-1α and EGFR are key molecular event during development and progression of laryngeal cancer, which act in regulating tumor angiogenesis as well, and show intimate relationship with biological behavior and prognosis of laryngeal cancer.
10.Expression of SLP-2 protein in esophageal squamous cell carcinoma is associated with cancer invasion.
Wen-feng CAO ; Li-yong ZHANG ; Bin ZHANG ; Yue-qi WANG ; Zhi-hua LIU ; Bao-cun SUN
Chinese Journal of Oncology 2010;32(11):830-833
OBJECTIVETo study the expression of stomatin-like protein-2 (SLP-2) in esophageal squamous cell carcinoma (ESCC), and analyze the correlation between SLP-2 expression and clinicopathological features.
METHODSThe expression of SLP-2 protein in ESCC tissues (18 and 220 cases respectively) was detected by Western blot and IHC. The association between SLP-2 expression and clinicopathological features was analyzed.
RESULTSCompared with normal epithelium, 13 cases of ESCC tissues showed a higher expression of SLP-2 on the protein level (72.2%, 13/18). IHC analysis on tissue microarray revealed that the expression rate of SLP-2 protein in ESCC was 54.1% and in normal esophageal mucosa was 3.6%, showing a significant difference (P < 0.001). SLP-2 high-level expression correlates with the extent of ESCC invasion (P = 0.033), but not with other clinicopathologic characteristics (P > 0.05).
CONCLUSIONSLP-2 as a novel cancer-related gene may play an important role in tumorigenesis of ESCC. The overexpression of SLP-2 may be closely associated with the invasion of esophageal cancer.
Adult ; Aged ; Aged, 80 and over ; Blood Proteins ; metabolism ; physiology ; Blotting, Western ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Esophageal Neoplasms ; metabolism ; pathology ; Female ; Humans ; Immunohistochemistry ; Lymphatic Metastasis ; Male ; Membrane Proteins ; metabolism ; physiology ; Middle Aged ; Neoplasm Invasiveness