Objectives To investigate the effect of carbon dioxide pneumoperitoneum on postoperative cognitive dysfunction and the level of serum NSE and S-100β protein in female patients undergoing gynecological laparoscopy. Methods 60 ASA physical status Ⅰ patients were divided two groups, group Ⅰ received no insufflation andconventional abdominal surgery ( n = 30) and group Ⅱ received abdominal insufflation and gynecological laparoscopy ( n =30). MMSE was recorded at several different time points, including one day before operation, 1, 6, 24, 48, 72h after operation, and before discharge. Serum S-100β protein and NSE was measured by ELISA before the beginning of operation ( or carbon dioxide pneumoperitoneum) and 1h after operation (or carbon dioxide pneumoperitoneum). Results MMSE values at 1,6,24,48,72h decreased significantly in group Ⅱ (24. 67 ± 1.47,25.97 ± 1.50,26. 77 ± 1.61,27.07 ± 1.87,27.37 ± 2. 06) after operation, compared with group Ⅰ (27.63 ± 1. 33,27.27 ± 0. 87,28.37 ± 0. 85,28.73 ±0. 78,29. 23 ±0. 86, P <0. 01 ). And the baseline value (29. 17 ±0. 76) of serum S-100β[(0. 114 ±0. 012,0. 086 ±0. 009) μg/L] protein and NSE [( 13. 720 ± 1. 330,12. 093 ±0. 697) μg/L] increased significantly at 1h after operation in group Ⅰ and Ⅱ compared with before operation [(0. 035 ±0. 030,0. 035 ±0.024;5.753±0.889,5.831 ±0.967)μg/L, P <0.01]. Serum S-100 β protein[(0. 114 ±0.012) μg/L] increased significantly at 1h after operation in group Ⅱ, compared with group Ⅰ [(0. 086 ±0. 009) μg/L,P < 0. 05], whereas NSE showed not difference [( 12. 093 ± 0. 697,13. 720 ± 1. 330) μg/L, P > 0. 05].Serum of S-100β protein and MMSE were significantly correlated w group Ⅰ and Ⅱ ( r = 0. 6412,0. 8126, P <0.01). Serum NSE was not correlated with the MMSE score in group Ⅰ ( r =0.4397, P >0.05),whereas NSE and MMSE had significant correlation in group Ⅱ ( r = 0. 7111, P <0. 01 ). Conclusions Carbon dioxide pneumoperitoneum in patients with gynecological surgery might affect postoperative cognitive function, and MMSE score was negatively correlated with serum S-100β and NSE proteins.

[Objective] Pharmacological actions of polysaccharides from Fructus Rosae Roxburghii (PFRR) were investigated to supply evidence for its source development and application. [Methods] With ginsenoside as the positive control, the effect of PFRR on the swimming time and the survival time under the conditions of normotensive hypoxia, hyperther-mia and hypothermia in mice were observed. Phagocytic function of macrophage and serum hemolysin level were also detected. [Results] In PFRR group, the swimming time and the survival time under the above conditions were both prolonged, phagocytic percentage and phagocytic index increased and time at 50% hemolytic concentration prolonged ( P

Dysprosium ; Endophthalmitis ; etiology ; Humans ; Quartz

Objective: To explore and obtain the isolation and culture methods of the highly purified primary rat brain microvascular endothelial cells (BMECs). Methods: The brains of 10 3-week-old Wistar rats were harvested by decapitation, removing the white matter and mincing into approximately 1 mm3. A highly purified brain microvascular fragments were obtained through enzymatic digestion twice, 20% bovine serum albumin (BSA) and 33% continuous Percoll density gradient centrifugation. They were incubated into gelatin-coated 35 mm dish plates and the endothelial medium containing 4 μg/ml puromycins was added. The medium was renewed after 2 days. The endothelial cell growth and its morphology were observed using an inverted microscope. The expression of BMECs markers factor VIII-related antigen (vWF) was detected by immunocytoehemical method and was identified. Results: The endothelial cells grew out around the adherent brain microvascular fragments after culturing for 24 hours, and the cells were spindle-shaped. A fusion was formed after 5 to 6 days. BMECs after the fusion showed a typical "cobblestone-like" appearance. Immunocytochemical analysis showed the vWF expression of BMECs was positive, while the glial fibrillary protein (GFAP) expression was negative. The purity of BMVECs reached more than 96%. Conclusion: This method may successfully obtain the highly purified rat primary BMECs.

OBJECTIVE:To observe clinical efficacy and safety of tanshinone ⅡA sulfonate combined with alprostadil in the treatment of acute cerebral infarction. METHODS:56 patients with acute cerebral infarction were randomly divided into treatment group(36 cases)and control group(20 cases). Control group was given tanshinone ⅡA sulfonate 60 mg intravenously,qd,for 10 days. Treatment was additionally given Alprostadil injection 10 μg intravenously,30 min drop lrote,qd,the two group for 10 days,on the basis of control group. Therapeutic efficacy and BI were observed at the end of a treatment course. RESULTS:The ef-fective rate of treatment group and control group were 91.67% and 75.00%,with statistical significance(P<0.05). BI score of 2 groups after treatment were better than before,and the treatment group was better than the control group after treatment,with statis-tical significance (P<0.05). No obvious ADR wad found in both groups. CONCLUSIONS:Tanshinone ⅡA sulfonate combined with alprostadil is effective in the treatment of acute cerebral infarction with good safety.

Objective To investigate the oxidative mechanism of homocysteine ( Hey) -induced apoptosis in endothelial progenitor cells( EPCs). Methods Total mononuclear cells were isolated from mouse bone marrow by Ficoll density gradient centrifugation and were cultured in vitro for 7 d. Adherent cells were harvested and identified by fluorescence microscopy. EPCs were cultured with Hey (0, 50, 100 and 500 μmol/L) for 12, 24 and 48 h, or pretreated with NAC (1 mmol/L), DPI( 10 μmol/L) or SB203580 (10 μmol/L) for 30 min, then cultured with 500 μmol/L Hey for 24 h. Apoptosis was detected by Annexin-V/PI flow cytometry, levels of reactive oxygen species (ROS) in cells were measured using H2DCF-DA as a fluorescence probe, NADPH oxidases were evaluated with lucigenin-enhanced chemilumine9cence, and NO in the supernatant was determined by nitrate reductase assay. Results Hey induced EPCs apoptosis, ROS accumulation, NADPH oxidase activation and decrease of NO in a time-dose dependent manner( P <0.05 or P < 0.01). Pretreatment with NAC, DPI and SB203580 could inhibit these effects (P < 0.05 or P < 0.01). Conclusion Hey could activate NADPH oxidase, induce ROS increase and NO decrease, and activate p38MAPK to enhance EPCs apoptosis.

Echinococcosis is a zoonotic parasitic disease caused by infection of Echinococcus granulosus and Echinococcus multilocularis in the human body,which may endanger the health and life of patients.Surgical treatment is presently the main method for the treatment of echinococcosis,with drug therapy as a subsidiary measure.The paper summarizes the advances in the treatment of echinococcosis.

Actins ; genetics ; Adolescent ; Cardiomyopathy, Dilated ; genetics ; Child ; Child, Preschool ; Desmin ; genetics ; Female ; Humans ; Infant ; Male ; Mutation

Objective To investigate the relationship among serum leptin level in patients with type 2 diabetes and sex , serum glucose, serum insulin, body mass index (BMI), waist-hip ratio (WHR) and total cholesterol and triglyceride.Methods 56 cases of type 2 diabetes and 22 normal controls were attended in this study. BMI,WHR, fasting serum leptin, fasting and 2h postprandial serum glucose and insulin, total cholesterol and triglyceride were determined.Results The average serum leptin level in women was significantly higher than that in men (P

Objective To study the effects of Levodopa on the abnormal behavior, nigral antioxidation system, mitochondrial respiration-chain function, neurotransmitter metabolism of Parkinson's disease (PD) model rat and its mechanism.Methods The rat models of PD were established through stereotaxic microinjection of 6-hydroxydopamine (6-OHDA). The rats in levodopa group were given levodopa 25 mg/(kg?d) through intragastric administration for 45 days. All the rats’behavior was tested before and after giving medicine. The activity of glutathione peroxidase(GSH-Px) and ROS, contents of malondialdedyde(MDA) and mitochondrial respiratory chain enzyme complex Ⅰ in nigra and the contents of dopamine(DA), homovanillic acid(HVA) and activity of monoamine oxidase-B(MAO-B) in caudate nucleus were assayed after treatment.Results (1) Compared with before treatment, the rotation rate after treatment decreased obviously( P