1.Effects of valproate acid combined with pirarubicin on childhood acute lymphoblastic leukemia cell line.
Bao-Lan SUN ; Li-Hui WU ; You-Jia WU
Chinese Journal of Hematology 2012;33(9):771-773
Apoptosis
;
drug effects
;
Cell Line, Tumor
;
Cell Proliferation
;
drug effects
;
Doxorubicin
;
administration & dosage
;
analogs & derivatives
;
pharmacology
;
Humans
;
Precursor Cell Lymphoblastic Leukemia-Lymphoma
;
drug therapy
;
pathology
;
Valproic Acid
;
administration & dosage
;
pharmacology
2.Observation on effect of qi-supplementing blood-activating and stasis-removing principle in treating patients with systemic lupus erythematosus of blood-stasis type.
Jia-li WU ; Xio-hui BAO ; Zhi-lin ZHOU
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(11):860-861
Adolescent
;
Adult
;
Anti-Inflammatory Agents
;
therapeutic use
;
Diagnosis, Differential
;
Dipyridamole
;
therapeutic use
;
Drug Therapy, Combination
;
Drugs, Chinese Herbal
;
therapeutic use
;
Female
;
Humans
;
Lupus Erythematosus, Systemic
;
drug therapy
;
Male
;
Medicine, Chinese Traditional
;
Middle Aged
;
Phytotherapy
;
Prednisone
;
therapeutic use
;
Tripterygium
;
chemistry
3.Protein arginine methyltransferase 1 methylates SF2/ASF at arginine
Hui JIA ; Chaohao DU ; Shilai BAO ; Huyong ZHENG
Chinese Journal of Cancer Biotherapy 1995;0(03):-
Objective:To investigate the arginine (Arg) sites in splicing factor 2/alternative splicing factor (SF2/ASF) methylated by protein arginine methyltransferase 1 (PRMT1). Methods:Wild-type and Arg93,Arg97,Arg109 mutant SF2/ASF plasmids were constructed,and GST-PRMT1,GST-SF2/ASF and arginine mutant GST-SF2/ASF fusion proteins were induced and purified. Methylation activity of PRMT1 on wild-type or mutant SF2/ASF protein and methylated sites of SF2/ASF were examined by methylation assay. The effect of SF2/ASF methylation on its subcellular localization was analyzed by immunofluorescence assay.Results:PRMT1 induced methylation of SF2/ASF at arginine,and PRMT1 did not methylate SF2/ASF when SF2/ASF was mutant at Arg93,Arg97 or Arg109,with Arg97 mutation showing the most profound inhibitory effect. Methylation of SF2/ASF did not affect its subcellular localization.Conclusion:SF2/ASF is a newly identified substrate of PRMT1; Arg93,Arg97 and Arg 109 are the three methylation sites in SF2/ASF,and Arg97 is the main methylation site. Methylation of SF2/ASF does not affect its subcellular localization.
4.Not Available.
Ze-Hao CHEN ; Jia-Kai HE ; Ran LI ; Yu-Hang JIANG ; Bao-Hui JIA
Chinese Acupuncture & Moxibustion 2023;43(12):1454-1456
5.In vitro effect of total flavones of Fructus Chorspondiatis on expression of collagen type I and type III mRNA and protein of cultured rat cardiac fibroblasts.
Jun-Ping BAO ; Ming JIN ; Yu-Min YANG ; Xiao-Hui GAO ; Liang SHU ; Hui-Hui XING ; Lei JIA
Acta Pharmaceutica Sinica 2014;49(1):136-141
This study aims to investigate the effect of total flavones of Fructus Chorspondiatis (TFFC) on the mRNA and protein expression of collagen type I and III of rat cardiac fibroblasts (CFs) induced by angiotensin II (Ang II), and explore its anti-myocardial fibrosis molecular mechanism. Neonatal rat CFs were prepared from Sprague-Dawley rats (1-3 d after birth). The expression of collagen type I and III mRNA and protein were measured by RT-PCR and Western blotting, respectively. The study showed that stimulation of neonatal rat CFs with 100 nmol.L-1 of Ang II for 72 h resulted in a significant increase of the expression of collagen type I and III mRNA and protein. The changes on the expression level were blocked by TFFC. The results demonstrated that TFFC can inhibit myocardial fibrosis induced by Ang II in rats, which is probably associated with the collagen type I and III mRNA and protein levels up-regulated by Ang II, and TFFC was shown to decrease the expression levels of collagen type I and III mRNA and protein.
Anacardiaceae
;
chemistry
;
Angiotensin II
;
pharmacology
;
Animals
;
Animals, Newborn
;
Cells, Cultured
;
Collagen Type I
;
genetics
;
metabolism
;
Collagen Type III
;
genetics
;
metabolism
;
Dose-Response Relationship, Drug
;
Drugs, Chinese Herbal
;
administration & dosage
;
isolation & purification
;
pharmacology
;
Fibroblasts
;
cytology
;
metabolism
;
Flavones
;
administration & dosage
;
isolation & purification
;
pharmacology
;
Fruit
;
chemistry
;
Myocardium
;
cytology
;
metabolism
;
Plants, Medicinal
;
chemistry
;
RNA, Messenger
;
metabolism
;
Rats
;
Rats, Sprague-Dawley
6.Comparative analysis of laser confocal microscopy and corneal smear in the diagnosis of fungal keratitis
Xiao-Hui, SUN ; Xin-Xin, LI ; Jia-Jun, ZHANG ; Bao-Shi, CHEN ; Li-Juan, LIU
International Eye Science 2017;17(6):1147-1149
AIM: To comparatively analyze the application of laser confocal microscopy and corneal smear in the diagnosis of fungal keratitis.METHODS: Totally 77 patients (77 eyes) diagnosed as fungal keratitis were selected.Laser confocal microscopy and corneal smear examination were performed to observe the characteristics of the images, and the detection rate of fungus were compared between the two methods.RESULTS: Of the 77 patients, 66 eyes (86%) were positive and 11 eyes were negative detected by laser confocal microscopy;51 eyes (66%) were positive and 26 eyes were negative detected by corneal smear examination, the difference was statistically significant compared between two group (P<0.05).CONCLUSION: Laser corneal confocal microscopy is relatively safe and sensitive.If combined the application of laser corneal confocal microscopy and corneal smear examination in clinical, the diagnosis rate of fungal keratitis may be improved.
7.Association between T245G polymorphisms in the osteoprotegerin gene and bone mineral density in elderly individuals
Lingxia CHEN ; Yide MIAO ; Jie LIU ; Yanan WEI ; Rong JIA ; Hui BAO ; Lin CHU
Chinese Journal of Tissue Engineering Research 2011;15(11):2069-2073
BACKGROUND: As a primary clinical predictor of fracture risk, bone mineral density (BMD) is partly genetically determined. Osteoprotegerin (OPG) is one important candidate gene in the pathogenesis of osteoporosis. OBJECTIVE: To investigate the association between T245G polymorphisms in the OPG gene and BMD. METHODS: A total of 281 elderly men and postmenopausal women, 182 males and 99 females, who received routine examinations at Peking University People's Hospital between September 2008 and April 2010 were included in this study. T245G polymorphisms in the OPG gene was detected by polymerase chain reaction-restriction fragment length polymorphism together with DNA sequencing. The BMD of the lumbar spine, Ward's triangle, and forearrm was determined by dual energy X-ray absorptiometry. Clinical variables and biochemical measurements were collected simultaneously. The association between T245G polymorphisms and each detection index was analyzed using analysis of variance. RESULTS AND CONCLUSION: The distribution of T245G genotype (alleles T, G) had no difference in elderly men or postmenopausal women (P > 0.05). The GG genotype and TG genotype had higher lumbar spine BMD and TT genotype had lower lumbar spine BMD (P < 0.05). There was no difference in BMD of the Ward's triangle or forearm among different genotypes (P > 0.05). Association between T245G polymorphism and BMD was not found in postmenopausal women. These findings indicate that OPG gene is related to lumbar spine BMD in elderly men.
8.Relationship between hearing threshold and peripheral neuropathy in type 2 diabetic patients
Juan SHEN ; Fang LIU ; Hui ZENG ; Jie YU ; Qiag LI ; Yuqian BAO ; Weipiag JIA
Chinese Journal of Endocrinology and Metabolism 2011;27(8):644-648
Objective In order to investigate the relationship between hearing loss and diabetic peripheral neuropathy( DPN ) via comparing the pure tone thresholds and vibration perception threshold(VPT) in type 2 diabetic patients and control person without diabetes. Methods 173 subjects including 138 type 2 diabetic patients(DM)and 35 non-diabetes controls were examined for VPT and hearing threshold. Nerve conducting velocity ( NCV )including sensory nerve conducting velocity( SCV )and motorial nerve conducting velocity( MCV )of diabetic patients were determined. The participants were divided into three groups: control group (n = 35 ), DM group without peripheral neuropathy( non-PN group, n = 74 ), and DM group complicated with peripheral neuropathy (PN group,n = 64 ). The clinical characteristics, biochemical parameters , the incidence of sensorineural hearing loss ( SNHL),pure tone threshold, and VPT were compared among three groups. At last, the relationship between hearing thresholds and NCV were analyzed. Results The incidence of hearing impairment of sensorineural type was 29.69% in PN group, which was significantly higher than that of non-PN group( 17.57% )and control group( 17. 14% ). There was significant differences in age, duration of diabetes, glycolated hemoglobin (HbA1c), glycolated serum albumin ( GA), Fasting blood glucose( FPG), 2h postprandial blood glucose( PPG), VPT, and hearing threshold among the three groups( all P<0. 05 ). The value of hearing threshold increased significantly( all P<0. 05 ) in 3 VPT subgroups with VPT≤ 15 V, VPT 16-25 V, and VPT >25 V. The Spearman correlation analysis showed median NCV was negatively correlated with hearing threshold on 1.00, 2.00, 4. 00, and 8. 00 kHz ( All P < 0. 05 ). The logistic regression analysis indicated that the age( regression coefficient =0. 088, P<0. 01 ) was the independent risk factor of SNHL, median nerve MCV ( regression coefficient = -0. 135, P = 0. 046 ) was the important influencing factor of SNHL. Conclusion Diabetic patients are more likely to suffer from impaired middle-frequency and high-frequency hearing, DPN in patients is often complicated with hearing impairment. Age and median nerve MCV were major risk factors of SNHL in diabetic patients.
9.Detection of mitochondrial membrane potential changes in Myelodysplastic syndrome by fluorescent probe JC-1
Guo-Hua XIA ; Bao-An CHEN ; Hui-Xia LU ; Ze-Ye SHAO ; Jia-Hua DING ; Chong GAO ;
Chinese Journal of Laboratory Medicine 2003;0(08):-
Objective To explore the function of fluorescent probe JC-1 in detecting the changes of mitochondrial membrane potential(△?m)in early apoptotic cells.Methods After 2-ME was used to induce MUTZ-1 cell apoptosis,cells were dyed with fluorescent probe JC-1,and then the changes of △?m in the early stage of apoptotic cells were analyzed by flow cytometry or detected under fluorescent microscope. Results The control cells with high △?m are those forming JC-1 aggregates in the inner membrane of mitochondria,thus showing orange-red fluorescence.2-ME caused decrease of △?m in MUTZ-1 cells,in which JC-1 maintains monomeric form,thus showing only green fluorescence.The decreases of △?m were in a time-dependent manner,which were significantly higher than those in control group(P
10.Research on spectral reflectance characteristics for Glycyrrhizae Radix.
Hui LI ; Cai-Xiang XIE ; Xiao-Jin LI ; Mei-Jia WEN ; Guang-Lin JIA ; Ming-Hui SHI ; Bao-Lin GUO ; Xiao-Guang JIA
China Journal of Chinese Materia Medica 2014;39(3):427-432
In order to study the spectral reflectance differences of Glycyrrhizae Radix under different growth conditions and lay the foundation for quantitative monitoring of Glycyrrhizae Radix remote sensing images, spectra of Glycyrrhiza species under different growth period and different varieties and different regions were measured by a portable spectrometer. The results showed that the reflectivity of annual G. uralensis was obviously higher than that of the two years plant in the visible light band own to the contents of crown layer chlorophyll. The reflectivity of two years G. pallidiflora was higher than that of G. uralensis in the near infrared band own to the leaf area index and the content of leaf water. The red edge spectrum of annual plant fluctuated largely than that of two years plant due to vegetation coverage and leaf area index. G. pallidiflora grew well than G. uralensis. Under different regions of the Glycyrrhiza species, spectral data analysis showed that within a certain range, the average annual precipitation and average annual evaporation were the major factors to affect the differences of Glycyrrhiza species spectral data under different regions owe to the leaf water content, the higher leaf water content, the lower spectral reflectance. The principal component analysis and continuum-removed method of the spectral data under different regions found that, within a certain range, the average annual precipitation and average annual evaporation were the major factors caused by the differences of Glycyrrhiza species spectral data under the different regions, Glycyrrhiza species spectral similarity related to the spatial distance.
Geography
;
Glycyrrhiza
;
chemistry
;
Principal Component Analysis
;
Spectrum Analysis