Objective To observe the therapeutic effect of external application and oral use of Herba Seu Radix Violae Japonicae(HSRVJ) for the treatment of chronic osteomyelitis.Methods Sixty chronic osteomyelitis were randomized into the treatment group and the control group.The two groups received conservative routine antibiotic treatment.The treatment group received external application and oral use of HSRVJ additionally.Seven days constituted one treatment course,and the treatment lasted 6 courses.The changes of erythrocyte sedimentation(ESR) and C-reactive protein(CRP) were compared in the two groups before treatment and every 2 courses.Meanwhile,the clinical cure rate and the total effective rate were observed in the two groups.Results The cure rate was 46.67% and the total effective rate was 86.67% in the treatment group,and was 20.00% and 63.33% in the control group respectively.ESR and CRP decreased gradually every 2 courses in the two groups(P

Our previous studies have confirmed that morroniside has neuroprotective effects. However, the effects of morroniside on cardiac myocardium remain unknown. Rats were anaesthetized with 10% chloral hydrate (0.35~0.4 mL/kg) and an acute myocardial infarction (AMI) was induced by ligating the anterior descending coronary artery (LAD). Following AMI, morroniside was administered intragastrically for 3 consecutive days at doses of 45, 90 and 180 mg/kg, respectively. Lactate dehydrogenase (LDH) and cardiac troponin T (cTnT) activities in AMI rats in the serum were detected with commercial kits. The expression of IL-6, IL-1β and TNF-α in myocardium was detected by Western blotting analysis. We observed a significant decline in the Q(q) wave amplitude in morroniside-treated rats after 72 h. Additionally, treatment of morroniside decreased the levels of LDH and cTnT in AMI rats. We also observed that morroniside reduced the expression of IL-6, IL-1β and TNF-α in myocardium. Taken together, our findings demonstrate that morroniside had effective anti-inflammatory properties in AMI rats.
Animals ; Blotting, Western ; Chloral Hydrate ; Coronary Vessels ; Inflammation ; Interleukin-6 ; L-Lactate Dehydrogenase ; Myocardial Infarction* ; Myocardium ; Neuroprotective Agents ; Rats* ; Troponin T