Objective To explore the influence of proton pump inhibitors (PPI)omeprazole on the platelet aggregation rate of clopidogrel in patients with acute coronary syndrome (ACS).Methods A total of 159 patients with ACS were selected,based on the standardized treatment,the patients were divided into the two groups according to the illness.65 cases in the treatment group were given omeprazole (20mg/d).94 cases in the control group were not given omeprazole.Both two groups were given aspirin(100mg/d) and clopidogrel (75mg/d).The platelet reactivity index(PRI) and clopidogrel active metabolites (clopi-H4)were detected five days later.Results After treatment for 5 days,the number of PRI ＞ 50％ in the treatment group and control group was 12 cases(18.5％) and 7 cases (7.4％) (x2 =4.431,P ＜ 0.05).The clopi-H4 levels in the treatment group and control group were 332.8 mmol/L (SD 371.8) and 600.3 mmol/L(SD 885.2) (t =-2.298,P ＜ 0.05).Conclusion In patients with ACS,combined omeprazole and clopidogrel can reduce the concentration of clopidogrel active metabolites clopi-H4,inhibition of clopi dogrel platelet aggregation.

Objective To observe the serum levels of Gal-3,Hs-CRP,IL-6 and TNF-α,and discuss the correlation between those indicators and the severity of coronary lesion and major adverse cardiac events( MACE). Methods Serum levels of Gal-3,Hs-CRP,IL-6 and TNF-α were detected in 126 patients with coronary heart disease and 54 patients with non-coronary heart disease. To analyze the correlation between those indicators and the severity of cor-onary lesion and MACE. Results Serum levels of Gal-3 and inflammatory factors of in the coronary heart disease group were significantly increased compared with the control group(P<0. 01),and serum levels of Gal-3 and in-flammatory factors in the multi-vessel disease group and the severe coronary disease stenosis group higer than that of the double-vessel disease group,the single-vessel disease group and the mild coronary disease stenosis group ( P<0. 05). Serum levels of Gal-3,inflammatory factors and Gensini scores were positively correlated(P<0. 01). Ser-um levels of Gal-3 , inflammatory factors were significantly higher with MACE compared to without MACE ( P <0. 05). Conclusion The serum levels of Gal-3 and Hs-CRP,IL-6,TNF-α,Gensini scores in patients with coronary heart disease are positively correlated,serum Gal-3 has great potential to become effective clinical indicators,which could be used to preliminary predict the severity of coronary artery disease and evaluate the short-term prognosis of patients with coronary heart disease.

Patients diagnosed to have acute coronary syndromes( ACS) were included in the study. All ACS patients were divided into non-diabetes group(40 cases) and diabetes group(80 cases) . Blood coagulation function was de-termined for all patients. In the diabetes group eighty patients were randomly divided into ticagrelor group (48 ca-ses) and clopidogrel group (32 cases) . After 5 days′treatment, platelet function was detected. Our study aimed to examine the effects of ticagrelor and clopidogrel on the platelet function.

Objective To observe the changes of serum cystatin C(Cys C) in patients with coronary heart disease(CHD) and normal kidney function ,and evaluate the predictive value of Cys C concentration on coronary arterial lesions .Methods Serum levels of Cys C were detected in 316 patients with coronary heart disease and normal renal function .The relationship between serum 1evels of Cys C and coronary heart disease was evaluated from three aspects :the number of diseased vessels ,the severity of diseased ves‐sels and the CHD Gensini scores .Results The Cys C level in CHD group was significantly higher than that of non‐CHD group [(1 .24 ± 0 .32)mg/L vs .(1 .12 ± 0 .27)mg/L ,P<0 .01] .Serum Cys C was significantly higher in the multi‐vessel disease group than that of the single‐vessel disease group[(1 .31 ± 0 .31)mg/L vs .(1 .20 ± 0 .32)mg/L ,P<0 .01] .Serum Cys C was significantly higher in the severe coronary artery stenosis group than in the mild stenosis group [(1 .29 ± 0 .36)mg/L vs .(1 .16 ± 0 .23)mg/L , P<0 .01] .Cys C was positively correlated with the Gensini scores of coronary arterial lesion (r=0 .195 ,P<0 .01) .Conclusion Cys C levels increased in patients with coronary heart disease and normal renal function .Cys C was positively correlated with the Gensini scores of coronary arterial lesion .Serum Cys C levels has certain predictive value on coronary arterial lesions in patients with CHD .

Objective To investigate the hospital mortality of rheumatic heart disease (RHD)and major cause of death. Methods One hundred and thirty patients with RHD prepared to take valve replacement from January 2006 to November 2009 were involved in this study. They were divided into two groups according to all clinical data: death group (10 cases ) and survival group (120 cases ). The clinical data and complications were collected, and the hospital mortality and their causes were analyzed with Logistic regression analysis. Results Univariate analysis: total bilirubin and indirect bilirubin levels in death group [(30.31 ± 19.10), (22.38 ± 17.34) μmol/L] were significantly higher than those in survival group [(19.47 ± 8.61),(12.92 ± 7.30) μmol/L] (P ＜ 0.01). The incidence of postoperative complications,history of cerebral infarction and cardiovascular complications in death group were significantly higher than those in survival group (P ＜0.01). The Logistic regression analysis showed that the postoperative complications was an independent risk factor for hospital mortality (P =0.002). Conclusions RHD with postoperative complications is identified as an independent predictor of hospital mortality. It is very important to enhance the treatment and care during hospitalization.

BACKGROUND:Hepatocyte growth factor(HGF) is a kind of multifunctional growth factor,which can accelerate cell growth,transition and genesis in various organs.In cardiovascular system,HGF has reported to have anti-apoptotic,anti-fibrotic,as well as repair endothelial cell injury effects,suppose that HGF has relationships with hypertension.OBJECTIVE:To investigate the effect of HGF on blood pressure,vascular endothelial system and renin-angiotensin system(RAS) in spontaneously hypertensive rats,further more,to discuss the mechanisms between HGF and hypertension.DESIGN,TIME AND SETTING:The randomized control experiment on animal was performed at the Department of Cardiology,First Affiliated Hospital of Anhui Medical University from March to July 2007.MATERIALS:The exogenous HGF power was purchased from America Peprotech Company.Rats with 14-weeks-old,weighting 200-250 g,were randomly divided into the experimental and spontaneous hypertension groups,WKY rats were serves as the control group,with 12 animals in each group.METHODS:Rats in the experimental group were administrated 5,10,15,20,and 25 ?g/kg HGF per 24 hours,the partes aequales sodium chloride was injected into the spontaneous hypertension and control groups.Five minutes after administration,systolic pressure and heart rates were measured.The rats were sacrificed and harvested 2 mL blood of right ventricle when blood pressure decrease the lowest,about 30 minutes after administration.MAIN OUTCOME MEASURES:The effect of HGF on systolic pressure and heart rates,and levels of serum nitrogen monoxidum(NO),plasma endothelin(ET),as well as angiotensinⅡ(Ag Ⅱ) were detected by colorimetry and radio-immunity methods.RESULTS:When injected 5?g/kg HGF,the blood pressure did not significantly change.After 10 ?g/kg HGF injection,the blood pressure began to decrease and reached a peak after 30 minutes,gradually recovered after 1 hour,and back to the normal after 5 hours.Injection of 20 ?g/kg has largest effect on rats,with reduced contractive pressure 40-50mmHg,but no significantly changes appeared by adding HGF dose.There were no significantly differences of heat rate and blood pressure between the two control groups.Compared with the spontaneous hypertension group,the levels of ET and AgⅡ were decreased,however,the level of NO was increased(P

Aim To evalute the effects of losartan on the cardiac function and secretion of nitric oxide(NO) and endothelin(ET) in patients with congestive heart failure(CHF) .Methods Sixty patients with class Ⅱ～Ⅳ CHF were randomised to receive a 12 weeks of routine therapy either with losartan(n ＝ 30)to be added from 25 mg to 50 mg daily or with enalapril(n ＝ 30) to be added from 2.5 mg to 5 mg daily. The cardiac systolic and diastolic funtion and the levels of NO and ET were observed before and after therapy respectively, with 30 normal control subjects seving as control. Results The abnormal cardiac systolic and diastolic function parameter were present in patients with CHF. The ET and NO levels in CHF patients were significantly higher than those in the control group(P＜0.05) . NYHA function class was improved in 85% of losartan-treated patients and in 84% of enalapril-treated patients after 12 weeks, as compared with cardiac function at baseline. ET and NO levels were significantly reduced(P＜0.01), NO/ ET ratio was increased (P＜0.05) after 12 weeks of therapy in two active groups. Conclusion The systolic and diastolic functions of patients with CHF are greatly improved by losartan treatment, which reverses the imbalance of NO/ET and it is suspected that this action may be another important mechanism of its treating heart failure.

Objective:This study evaluates the performance of chemiluminescence assay, which is designed to detect Hepatitis D Virus (HDV) Immunoglobulin G (IgG) antibodies.Methods:A comparative analysis was conducted among chemiluminescence anti-HDV IgG reagent, the magnetic particle-based domestic reagent A and domestic reagent B, and the Robo Gene HDV RNA kit, using 1909 HBsAg-positive plasma samples. This comparison aimed to delineate clinical specificity and detection accuracy. The anti-HDV IgG reagent precision was assessed at three different concentration levels following the Clinical Laboratory Standards Institute EP5-A2 guidelines. The specificity of the assay was validated using 200 HAV IgM positive, 545 HBsAg-positive but anti-HDV IgG-negative, 350 anti HCV positive plasma samples and 200 healthy human blood samples. Additionally, a concordance study was conducted with 545 HBsAg-positive and 37 anti-HDV IgG-positive plasma samples, comparing the anti-HDV IgG reagent against reagent A.Results:1 909 HBsAg-positive plasma samples were tested using 3 anti HDV IgG reagent and 1 HDV RNA reagent, 19 samples were identified as anti-HDV IgG-positive. The anti-HDV IgG demonstrated superior accuracy and specificity. The assay exhibited excellent precision, with intra-assay coefficient of variation (CV) values ranging from 1.57% to 4.30%, and inter-assay CV values between 1.71% and 4.67% for detecting samples at high, medium, and low concentration levels. Concordance with Reagent A showed consistent results in both positive and negative detections.Conclusion:In this study, the anti-HDV IgG reagent (chemiluminescence method) displayed outstanding specificity in detecting clinical samples and exhibited a high conformity rate with commercialized reagents, making it potentially suitable for screening anti-HDV IgG in HBsAg-positive samples.

As an important basis for lesion determination and diagnosis, medical image segmentation has become one of the most important and hot research fields in the biomedical field, among which medical image segmentation algorithms based on full convolutional neural network and U-Net neural network have attracted more and more attention by researchers. At present, there are few reports on the application of medical image segmentation algorithms in the diagnosis of rectal cancer, and the accuracy of the segmentation results of rectal cancer is not high. In this paper, a convolutional network model of encoding and decoding combined with image clipping and pre-processing is proposed. On the basis of U-Net, this model replaced the traditional convolution block with the residual block, which effectively avoided the problem of gradient disappearance. In addition, the image enlargement method is also used to improve the generalization ability of the model. The test results on the data set provided by the "Teddy Cup" Data Mining Challenge showed that the residual block-based improved U-Net model proposed in this paper, combined with image clipping and preprocessing, could greatly improve the segmentation accuracy of rectal cancer, and the Dice coefficient obtained reached 0.97 on the verification set.
Algorithms ; Delayed Emergence from Anesthesia ; Humans ; Image Processing, Computer-Assisted ; Rectal Neoplasms/diagnostic imaging* ; Tomography, X-Ray Computed

Objective:This study aims to evaluate the quality and explore the preliminary clinical applications of a domestically developed hepatitis D virus nucleic acid quantification reagent (abbreviated as"domestic HDV RNA reagent").Methods:The sensitivity and accuracy of the reagent were evaluated in accordance with the WHO HDV RNA international standard, employing the Bio-Rad CFX Opus 96 real-time fluorescence quantitative PCR analysis system. Serial dilutions of pseudo-viruses or cell culture-derived virus were used to determine the linear range of the domestic HDV RNA reagent. Specificity was assessed using positive samples of HAV, HBV, HCV infection, and HEV national reference materials. Precision was evaluated with samples at both high and low concentrations. In a comparative analysis, 30 HDV IgG positive samples were tested using both the domestic HDV RNA reagent and the RoboGene HDV RNA kit based on the ABI 7500 FAST DX system. The Pearson correlation coefficient (r) was used to examine the correlation between the two reagents.Results:The domestic HDV RNA reagent demonstrated a high sensitivity of up to 6 IU/ml, consistent with that of the comparator reagent. The calibration curve for WHO HDV RNA standards had a slope of -3.286, with an amplification efficiency of 101.6%. The linear detection range spanned from 10 to 10 8 IU/ml for eight HDV genotypes. The domestic HDV RNA reagent exhibited exceptional specificity, without cross-reactivity observed with HAV, HBV, HCV, or HEV. Accuracy assessments at five concentration levels met the required standards, with intra-assay precision coefficient of variation ( CV) ranging from 1.20% to 4.20%, and inter-assay precision CV from 1.20% to 7.90%. The detection results for HDV IgG positive samples were highly correlated with the comparator reagent ( r=0.984, P<0.001), achieving a diagnostic accuracy of 100% compared to sequencing results. Conclusion:In this study, the domestic HDV RNA reagent possesses excellent specificity, accuracy, precision, and a broad linear range, attaining a sensitivity level on par with international reagents of the same type.