OBJECTIVE: The aim of this study is to investigate the expression and clinical significance of 6-phosphofructo-1-kinase in nasopharyngeal carcinoma biopsy samples. METHOD: Sixty-one biopsy samples were detected, including 41 tissues samples from patients with nasopharyngeal carcinoma and 20 tissues samples from patients with chronic nasopharyngitis as control group. 6-phosphofructo 1 kinase mRNA expression was detected by RTPCR. RESULT: It was observed that the expression levels of 6 phosphofructo-1-kinase mRNA in nasopharyngeal carcinoma tissues were higher than in the chronic inflammatory tissues. And the expression levels in patients with lymph node metastasis were higher than without lymph nod metastasis. CONCLUSION 6-phosphofructo-1-kinase may be a marker in occurrence and metastasis of nasopharyngeal carcinoma.
Biomarkers, Tumor ; metabolism ; Carcinoma ; Female ; Humans ; Lymphatic Metastasis ; Male ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; metabolism ; pathology ; Phosphofructokinase-1 ; genetics ; metabolism ; RNA, Messenger ; metabolism

Aluminum ; blood ; Humans ; Spectrophotometry, Atomic ; methods

OBJECTIVE: To observe the expression changes of metadherin (MTDH) and matrix metalloproteinase-9 (MMP-9) in the laryngeal squamous cell carcinoma tissues and to investigate the significance. METHOD: The expression of MTDH and MMP-9 in 54 cases of laryngeal squamous cell carcinoma tissues(observation group) and 30 cases of para-carcinoma tissues (control group) was examined by immunohistochemical method, the correlation between them and their correlations with the clinicopathological parameters were analyzed. RESULT: The positive expression rates of MTDH in the observation group and control group were 64.8% (35/54) and 6.7% (2/30), respectively; the positive expression rates of MMP-9 in the observation group and control group were 70.4% (38/ 54) and 13.3% (4/30), respectively; and there was a statistically significant difference between two groups (all P < 0.01). In the laryngeal squamous cell carcinoma tissues, the expression of MTDH protein was related with degree of differentiation, lymph-node metastasis and TNM stage (all P < 0.05); and the expression of MMP-9 protein was related lymph-node metastasis and TNM stage (all P < 0.05). The expression of MTDH was positively correlated with MMP-9 in the laryngeal squamous cell carcinoma tissues (r = 0.371, P < 0.01). CONCLUSION The high expression of MTDH and MMP-9 was closely related to the occurrence, development and metastasis of laryngeal squamous cell carcinoma, joint detection of the two proteins was valuable for early diagnosis and prognosis of laryngeal squamous cell carcinoma.
Carcinoma, Squamous Cell ; diagnosis ; metabolism ; Case-Control Studies ; Cell Adhesion Molecules ; metabolism ; Cell Differentiation ; Head and Neck Neoplasms ; diagnosis ; metabolism ; Humans ; Laryngeal Neoplasms ; diagnosis ; metabolism ; Lymphatic Metastasis ; Matrix Metalloproteinase 9 ; metabolism ; Neoplasm Staging ; Prognosis ; Squamous Cell Carcinoma of Head and Neck

Virtual reality (VR) is a three-dimensional virtual technology which generates virtual world by computer simulation. VR provides users with the simulation of visual, auditory and tactile senses, so that users can observe things in the virtual space as in the real world. Electroencephalogram(EEG) is the direct reflection of the activation of neurons in the cerebral cortex. The combination of VR and EEG has become an important method of brain science research. However, the existing research lacks a systematic arrangement of combination of VR and EEG. In this paper, the related research of VR combined with EEG in the past 20 years were summarized, the corresponding experimental paradigm, research methods and results were introduced, and finally the future research directions of the combination of VR and EEG were prospected.

Objective We aimed to investigate the efficacy of laparoscopic total mesorectal excision (TME) for treatment of rectal cancer.Methods We studied 90 patients with rectal cancer admitted to our hospital between March 2012 and March 2014.Patients enrolled for the study were divided into an experimental and control group.The control group underwent a TME procedure,while laparoscopic TME was performed in the experimental group.Efficacy of procedures performed and stress response indices were compared between the two groups.Results The bleeding volume,postoperative anal exhaust time,and duration of hospitalization were significantly lower in the experimental group than that noted in the control group (P ＜ 0.05),as was the incidence of complications (P ＜ 0.05).Assessment of stress indicators showed that the postoperative day 1 (1d) and day 3 (3 d) values were increased in the control group,while the experimental group showed significantly lower values across the same time frame (P ＜ 0.05).Conclusion Laparoscopic TME can reduce the incidence of complications in patients with rectal cancer,and reduce the stress response,which is beneficial for postoperative recovery of patients,and must therefore be promoted as a feasible treament option in patients with rectal cancer.

OBJECTIVE: The purpose of this article is to improve the understanding of complications of pleomorphic adenocarcinoma, and the possibility of complicated with dermatomyositis. In order to explore whether there is a certain relationship between the two diseases. METHOD: Reported one patient in our hospital with the diagnosis of pleomorphic adenocarcinoma complicated dermatomyositis, and clarified the diagnosis and treatment of these two diseases during hospitalization. Reviewed the latest clinical literature relevant to pleomorphic adenocarcinoma and dermatomyositis. RESULT: The patients underwent superficial parotidectomy treatment. The postoperation pathologic and immunohistochemical examination confirmed the diagnosis of pleomorphic adenocarcinoma. Dermatomyositis improved better after the operation. CONCLUSION Pleomorphic adenocarcinoma has the possibility of complicated with dermatomyositis which may misdiagnosis in clinical practice. Summarize the latest diagnosis progress of pleomorphic adenocarcinoma and the surgical treatment of the tumor. Also discuss the standards of clinical diagnosis and treatment of dermatomyositis. Analysis there may be a relationship between the two diseases on autoimmune mechanism.
Adenoma, Pleomorphic ; complications ; surgery ; Aged ; Dermatomyositis ; complications ; Humans ; Male

OBJECTIVE:To mine the ADR signals of dasatinib and imatinib,and to provide reference for safe use of two drugs in clinic. METHODS:ROR and PRR method of disproportionality measures were used to mine the data in the reports about dasatinib and imatinib of 17 quarters from FDA ADE Reporting System during the fourth quarter of 2012-the fourth quarter of 2016. ADR description terms in reports were standardized with international medical terms dictionary. ADR with signal detected by both methods were screened again. RESULTS & CONCLUSIONS:Totally 505 ADR signals for dasatinib and 929 ADR signals for imatinib had been found by ROR and PRR. After re-screening,there were 351 ADR signals for dasatinib and 649 ADR signals for imatinib,including 153 ADR signals for both dasatinib and imatinib. ADR signals for both dasatinib and imatinib obtained in this study were in agreement with known safety information. ADR mainly occurred in gastrointestinal tract,blood and lymphatics,kidney and urinary system,cardiovascular and musculoskeletal tissue,etc. However,incomplete information in the instructions was also found,such as possible urinary system-related ADR signals caused by dasatinib were detected in this study is not mentioned in drug instruction;imatinib could cause ADR signals of left atrium and right atrium dilation,which were not included in their instructions. Common ADRs,such as headaches,metioned in drug instructions of imatinib,did not appear in the top 50 signal intensities in this study. In addition,the ADR of the two drugs also varied,such as 7 ADR signals as periorbital edema and ocular edoma of forimatinib were much stronger than dasatinib;5 ADR signals of dasatinib,such as pericardial effusion and pleural effusion were much stronger than imatinib,indicating clinical drug selection should be based on individual situation of patients.

OBJECTIVETo investigate the transcriptional regulation of pacemaker channel I(f) mediated by vasoactive peptide endothelin-1 (ET-1) in neonatal rat ventricular myocytes and its mechanism.

METHODSNeonatal rat ventricular myocytes were enzymatically isolated. I(f) current was recorded using the whole-cell patch-clamp technique. The expression of hyperpolarization-activated cyclic nucleotide-gated channel (HCN) isoforms HCN2 and HCN4 were measured by quantitative RT-PCR.

RESULTSET-1 increased the expression of HCN2 and HCN4 mRNA in a dose- and time-dependent manner. These effects were blocked by specific ETA receptor antagonist BQ-123 but not the ETB receptor antagonist BQ-788. The effects of ET-1 on HCN2 and HCN4 mRNA expression were not affected by the p38 mitogen-activated protein kinase (MAPK) inhibitor (SB-203580).

CONCLUSIONThese findings indicate that ET-1 stimulates the expression of pacemaker channel I(f) in cardiomyocytes via ETA receptor through a p38 MAPK-independent signaling pathway, which might be linked to the intrinsic arrhythmogenic potential of ET-1.

Animals ; Animals, Newborn ; Cyclic Nucleotide-Gated Cation Channels ; drug effects ; Endothelin-1 ; metabolism ; Imidazoles ; pharmacology ; Myocytes, Cardiac ; drug effects ; metabolism ; Oligopeptides ; pharmacology ; Patch-Clamp Techniques ; Piperidines ; pharmacology ; Pyridines ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Signal Transduction ; drug effects ; p38 Mitogen-Activated Protein Kinases ; metabolism

Objective Through the 243 cases of hemorrhagic fever with renal syndrome(HFRS) of multiple organ failure after more investigation to explore the impact of various factors and generate sequelae.HFRS caused MSOF rescue guidance,to develop a variety of measures to reduce complications.Methods 243 cases of various forms on a return visit to investigate the implementation of the relevant auxiliary examination,using a large sample count data U test and the sample mean T test,combined with the original medical records were analyzed.Results HFRS patients cured,the incidence of sequelae of 47.73％,the incidence of multiple system damage 39.15％,in each case up to three system damage sequelae,its incidence and disease,incentives,diagnosis time,circulatory failure correct time,the use of Losec and blockers,age,number of organ failure,organ failure,and the starting number of central nervous system dysfunction and other closely related.Conclusion Gastrointestinal dysfunction is the initial factor MSOF,two organ failure is the starting signal severe illness,central nervous system dysfunction is in critical condition and the prognosis is poor marks.

Objective:To investigate secondary factors, laboratory features, treatment options, and prognosis of pure red cell aplastic anemia (PRCA) .Methods:This was a multicenter retrospective clinical study. Patients aged above 18 years newly diagnosed with PRCA between June 1, 2010, and June 1, 2019, were recruited as the main study object. A comparative analysis of remission rate and overall survival rate was made according to different treatment schemes adopted by patients and different drug reduction rates.Results:A total of 67 patients with PRCA were included in this study and the secondary PRCA group accounted for 44.8% (30/67) . The most common secondary factors were thymoma ( n=10) and T-cell large lymphocytic leukemia (T-LGLL) ( n=6) . The overall response rate of PRCA was 85.7% and the 3-year overall survival rate of PRCA was (74.3±7.5) %. The remission rate of cyclosporine A alone was slightly higher than that of oral glucocorticoid alone or combined with glucocorticoid[90.0% (36/40) vs 75.0% (12/16) , P=0.147]. After patients applied with cyclosporine A treatment reached CR/PR and remained stable for 3-6 months, the dose of cyclosporine A was reduced by 25 mg each time. The cyclosporine A reduction interval of a 25 mg/d reduction in more than 1 month significantly prolonged the median disease-free survival compared with a 25 mg/d reduction in less than 1 month [not reached vs 15 (95% CI 7-23) months, P<0.001]. There were 62.5% (10/16) of patients who responded to the initial or incremental treatment regimen after relapse. Conclusion:PRCA has features of various secondary factors, high overall survival rate, and high remission rate. Treatment with cyclosporine A alone is preferred, and cyclosporine A should be slowly tapered to reduce the risk of later relapse after it takes effect and patients reach a steady state.