Animals ; Conditioning, Classical ; Evoked Potentials, Somatosensory ; Fear ; psychology ; Male ; Rats ; Rats, Sprague-Dawley ; Stress, Psychological

Coronary Vessel Anomalies ; diagnosis ; Electrocardiography ; Humans ; Infant ; Pulmonary Artery ; abnormalities

Nasal polyp (NP) is a common chronic inflammatory disease of the nasal cavity and sinuses.Although some authors have suggested that NP is related to inflammatory factors such as interleukin (IL)-1β,IL-5,IL-8,granulocyte-macrophage colony-stimulating factor (GM-CSF),tumor necrosis factor (TNF)-α,and IL-17,the mechanisms underlying the pathogenesis and progression of NP remain obscure.This study investigated the expression and distribution of IL-17 and syndecan-1 in NP,and explored the roles of these two molecules in the pathogenesis of eosinophilic chronic rhinosinusitis with nasal polyps (Eos CRSwNP) and non-Eos CRSwNP.Real-time PCR and immunohistochemistry were used to detect the expression of IL-17 and syndecan-1 in samples [NP,unciform process (UP) from patients with CRS,and middle turbinate (MT) from healthy controls undergoing pituitary tumor surgery].The results showed that the expression levels of IL-17 and syndecan-1 were upregulated in both NP and UP tissues,but both factors were higher in NP tissues than in UP tissues.There was no significant difference in IL-17 levels between the Eos CRSwNP and non-Eos CRSwNP samples,and syndecan-1 levels were increased in the non-Eos CRSwNP tissues as compared with those in Eos CRSwNP tissues.In all of the groups,there was a close correlation between the expression of IL-17 and syndecan-1 in nasal mucosa epithelial cells,glandular epithelial cells,and inflammatory cells,suggesting that IL-17 and syndecan-1 may play a role,and interact with each other,in the pathogenesis ofnon-Eos CRSwNP.

Objective To assess the relationship between fractional esterification rate of high density lipoprotein cholesterol(FERHDL)and coronary artery disease.Methods A total of 131 hospitalized patients underwent coronary angiography due to chest pain were included in the study and Datients were divided into CAD group(n=76)and non CAD group(n=55)according to coronary allgiograrn.Clinical and laboratory data including biochemical laboratory,FERHDL and lipid subclasses were analyzed.Results The FERHDL value of CAD group was significantly higher than that of the non CAD group(21.70±8.73 vs.18.65 4±6.26,P<0.05).There was an increased trend of FERHDL with numbers of diseased coronary arteries,significant difference was evidenced between non CAD group and 3-vessel group(18.65±6.26 vs.24.00±9.22,P<0.05).FERHDL was positively correlated with TG(r=0.647,P<0.001).LDLb-C(r=0.441,P<0.001)and negatively correlated with HDL-C(r=-0.708,P<0.001)and HDL2-C(r=-0.748,P<0.001).Conclusion Our data showed that the values of FERHDL were significantly increased in CAD patients and correlated with the severity of the CAD.

Objective To investigate the early diagnosis,treatment and prognosis of pyriform sinus perforation.Methods Fifteen patients were diagnosed to have pyriform sinus perforation,most of them were associated with pharyngeal pain or fever.Contrast X-ray and CT scan were used to confirm the perforation.Results Conservative treatment was used in 9 cases with wild symptoms and surgical intervention in 6 cases with severe symptoms and complications.Both methods achieved better clinical efficacy.By relevant examination,the perforation healed within 2-4 weeks and none had re-perforation for 1-30 months follow-up.Conclusions Pyriform sinus perforation with wild symptoms and within 12 hours can be treated conservatively.Large perforation(＞ 2 cm)last more than 12 hours or with any complications require exploration,surgical repair if possible and adequate drainage.Early diagnosis and effective treatment can reduce the incidence of complications and improve the survival rate of patients.

Objective To treat the patients with anterior spaces caused by periodontal diseases with aligners and evaluate the periodontal conditions before and after treatment.Methods Seven patients with anterior spaces ranged from 3.0 to 4.5 mm were randomly selected.All the patients were treated with aligner technique to close the spaces.Bleeding on probing(BOP)and probing depth(PD)were measured before treatment,1 month,3 months and 6 months after treatment.The CEJ-ABC distance was evaluated before treatment,1 month,and 6 months after treatment.Results No significant differences were found in periodontal evaluation and the CEJ-ABC distance between any two evaluated stages.Conclusions The aligners could be used in patients with anterior spaces caused by periodontal diseases.No periodontal tissue damages were found during the observation period.

OBJECTIVETo investigate the expression of miR-122 and its relationship with progression and development of acute liver failure in mice induced by D-GalN/LPS, and to explore new biomarker(s) for early diagnosis of acute liver failure.

METHODSBALB/C mice were randomly divided into four groups: the mice were given D-GalN (900 mg/kg body weight) and LPS (10 micog/kg body weight) intraperitoneally (i.p.) to construct the acute liver model; whereas the control groups were given D-GalN (900 mg/kg), LPS (10 microg/kg) and normal saline respectively. All biochemical and histological indexes were determined at 0, 1, 3, 5, 7 and 9 h respectively after administration. Real-time RT-PCR were used to detect the expression of miR-122 and pro-inflammatory cytokines, furthermore, the expression of miR-122 was verified by LNA (lock nucleic acid)-Northern-blot. ALT and AST levels were tested by biochemistry analyzer. Serum pro-inflammatory cytokine levels were tested by ELISA.

RESULTSThe mortality rate was about 80% at 24h after D-GalN/LPS treatment, but no mortality was observed in the other three control groups. Liver special miRNA miR-122 was highly expressed in liver tissue of normal mice (ct is approximately equal to 14), it was up-regulated significantly (P = 0.013) at first hour after treatment then down-regulated according to the development of acute liver failure, the change was more obvious at 9 h (ct is approximately equal to 15, P = 0.002). ALT and AST levels increased obviously at 3h after treatment and reached peak at 7 hours then they were declined sharply. It was found that the expression of miR-122 was faster and more durable than ALT. Pro-inflammatory cytokines related to acute liver failure including TNFa and IL-6 were all up-regulated in serum as well as liver tissue (P less than 0.05). Correlation analysis showed that miR-122 had a negative correlation with ALT (correlation coefficients -0.505) and positive correlations with TNFa and IL-6 (correlation coefficients were 0.493 and 0.674 respectively).

CONCLUSIONSLiver-specific miR-122 supposed be a new marker molecule for early diagnosis of liver cells injury in the acute liver failure.

Animals ; Galactosamine ; adverse effects ; Interleukin-6 ; metabolism ; Lipopolysaccharides ; adverse effects ; Liver Failure, Acute ; chemically induced ; metabolism ; pathology ; Male ; Mice ; Mice, Inbred BALB C ; MicroRNAs ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism

OBJECTIVETo retrospective analysis operating procedures and results of calcaneal fractures treated with plate fixation, identify problems and propose measures to reduce surgical complications.

METHODSFrom June 2008 to April 2009, 156 patients were reviewed, including 121 males and 35 females, ranging in age from 18 to 59 years, with an average of 41 years. Thirty-two patients had bilateral calcaneal fractures. Twenty-three patients were surgically treated on both calcaneus at the same time. Total 179 calcaneus were treated with operation. The operation records of all patients were analyzed in order to find the problems and deficiencies.

RESULTSAmong 179 calcaneal fractures, 31 feet (17.3%) had postoperative wound problems: wound infection and non-healing were found in 26 feet (14.5%), skin flap necrosis in 5 feet (2.8%). There were a lot of problems of plate internal fixation for the treatment of calcaneal fractures, including operation design, operative technique, as well as fracture reduction and fixation.

CONCLUSIONThe following methods are the only way to avoid operative complications for the treatment of calcaneal fractures: improved surgical technique, minimally invasive operation, simple and effective internal fixation.

Adolescent ; Adult ; Calcaneus ; injuries ; surgery ; Female ; Fracture Fixation, Internal ; methods ; Fractures, Bone ; surgery ; Humans ; Male ; Middle Aged ; Young Adult

OBJECTIVETo investigate the difference of galectin-3 and CD44v6 expression between benign and malignant thyroid nodules, and to evaluate their clinical value in distinguishing thyroid cancer from benign thyroid nodules.

METHODSThe expression of galectin-3 and CD44v6 was immunohistochemically detected by the ABC method in 143 benign and malignant thyroid nodule samples.

RESULTSExpression of these two markers in benign thyroid nodules: galectin-3 was negative in 10 cases of para-cancer normal tissue and 14 cases of benign nodules found in the other benign thyroid disease. It was weakly positive in 4 of 52 nodular goiter (7.7%). Also it was weakly positive in 2 of 22 follicular adenomas (9.1%). But all three eosinophilic follicular adenomas were diffusely or focally positive for galectin-3. CD44v6 was negative in 10 cases of para-cancer normal tissue, but positive in 4 of 14 nodular lesions found in benign thyroid diseases (28.6%). It was also positive in 16 of 52 nodular goiters (30.8%), and weakly positive in 7 of 22 follicular adenomas (31.8%). The two markers in malignant lesions: galectin-3 was positive in 50 of 52 thyroid adenocarcinoma (96.2%), CD44v6 was positive in 42 of 52 thyroid adenocarcinoma (80.8%). The positive rate of galectin-3 and CD44v6 expression in thyroid cancer was significantly higher than that in benign thyroid nodule and normal tissue (P < 0.001). The sensitivity, specificity and accuracy of galectin-3 combined with CD44v6 in differentiating benign from malignant thyroid nodule were 80.8%, 93.4%, 88.8%; they were 96.2%, 90.1%, 92.3% for Galectin-3 alone.

CONCLUSIONThe immunohistochemical expression of galectin-3 and CD44v6 by the ABC method is significantly higher in thyroid cancers than in benign thyroid nodules, especially galectin-3 in thyrocyte being helpful in differentiating benign thyroid nodule from thyroid cancer.

Adenocarcinoma, Papillary ; diagnosis ; metabolism ; Adenoma ; diagnosis ; metabolism ; Biomarkers, Tumor ; biosynthesis ; Diagnosis, Differential ; Female ; Galectin 3 ; biosynthesis ; Glycoproteins ; biosynthesis ; Humans ; Hyaluronan Receptors ; biosynthesis ; Immunohistochemistry ; Male ; Thyroid Neoplasms ; diagnosis ; metabolism ; Thyroid Nodule ; diagnosis ; metabolism

OBJECTIVETo establish a convenient realtime PCR which can detect microRNAs in the human hepatoma cell line, Huh7 cells.

METHODSTotal RNAs in Huh7 cells were extracted. MicroRNA 122, 24 and 146a were assayed by microRNA array, and then verified by Northern blot. Stem-loop RT-PCR and poly(A)-tailed RT-PCR were used to detect the above microRNAs. Data were analyzed with Quantity One software and 7500 system software.

RESULTSMicroarray signal intensity of microRNA 122, 24 and 146a in Huh7 cells was 2201.49, 410.20 and 4.70, whose relative expression was confirmed as 0.0383, 0.0249, 0.0001 through Northern blot. While the poly(A)-tailed RT-PCR might only measure microRNA 122, Stem-loop RT-PCR could detect microRNA 122, 24 and 146a, whose average dCt was 2.5, 5.8 and 12.1 in accordance with microRNA array and Northern blot.

CONCLUSIONStem-loop RT-PCR can specifically and sensitively quantity microRNA levels, regardless of their abundance.

Base Sequence ; Blotting, Northern ; Carcinoma, Hepatocellular ; genetics ; metabolism ; Cell Line, Tumor ; DNA Primers ; Gene Expression Regulation, Neoplastic ; Humans ; Liver Neoplasms ; genetics ; metabolism ; MicroRNAs ; analysis ; genetics ; metabolism ; Oligonucleotide Array Sequence Analysis ; Reverse Transcriptase Polymerase Chain Reaction ; methods ; Sensitivity and Specificity