Objective To explore salvia miltiorrhizaligustrazine injection on cerebral basal smooth muscle cells under hypoxia ischemia and com-pare the efficacy of salvia miltiorrhiza, ligustrazine and salvia miltiorrhi-zaligustrazine injection.Methods Select cerebral basal smooth muscle cells to study.Use of sodium dithionite ( Na2 S2 O4 ) were incubated with the cells caused by oxygen and glucose deprivation injury.The experi-ment will be divided into control group ( fresh medium) , model group ( a lack of oxygen liquid) , salvia miltiorrhizaligustrazine low, middle, high-dose groups (0.01, 0.1, 1 μmol· L-1, 6 h), salvia miltiorrhiza group (0.1 μmol· L-1, 6 h), ligustrazine group(0.1 μmol· L-1, 6 h), salvia miltiorrhiza plus ligustrazine group ( 0.1 μmol · L-1 , 6 h ).Observe the general morphology of cerebral basal smooth muscle cells and morphological changes of apoptosis related, detection of cerebral basal smooth muscle cells survival, cerebral basal smooth muscle cells activity of extracellular lactate dehydrogenase ( LDH) and the activity of Bcl-2/Bax expression. Results Salvia miltiorrhizaligustrazine injection reduce hypoxia-reperfusion injury of cerebral basal smooth muscle cells in the general form.Compared with model group, it improve the survival rate of cerebral basal smooth muscle cells ( P<0.05 or 0.01 ) , increase the ratio of Bcl-2/Bax ( P<0.05 ) , and decrease the oxygen damage caused by extracellular LDH increased(P<0.05).Conclusion Salvia miltiorrhizaligustrazine in-jection has protective effect on the injury of cerebral basal smooth muscle cells induced by ischemia and hypoxia.Its protective effect superior to that with salvia miltiorrhiza andligustrazine.

OBJECTIVETo survey the distribution of influenza A subtypes in external environment and investigate the infectious status of highly pathogenic avian influenza (H5N1) in poultry-exposed population in Wuhan.

METHODSSeventy-eight external environmental samples (water, cage surface and fecal samples) were collected from 3 habitats of wild migratory birds and 5 urban live-poultry markets in 2010. In 13 avian influenza monitoring points, 249 serum samples were collected from people living around habitats of wild migratory birds or working in live poultry markets. Real-time RT-PCR method was adopted to detect influenza A virus from external environmental samples; and multiple RT-PCR method and specific H3, H5, H7 and H9 primers were then applied to analyze the subtypes of the positive samples. The levels of H5N1 antibody in poultry-exposed population were tested by horse hemagglutination inhibition test and two avian influenza inactivated antigens: A/Hubei/1/10 and A/Anhui/1/05.

RESULTSOf the 50 external environmental samples collected from live poultry markets, 17 samples were determined to be influenza A virus positive (positive rate 34.0%), including specific subtypes as follows: 4 samples of H5 single-positive subtype, 3 samples of H9 single-positive subtype, 4 samples of H3 and H5 mixed-positive subtype, 2 samples of H3 and H9 mixed-positive subtype, 2 samples of H5 and H9 mixed-positive subtype, 2 samples of H3, H5 and H9 mixed-positive subtype, but no H7 positive subtype was found. The 28 external environmental samples collected from habitats of wild migratory birds were all influenza A virus negative. Considering different types of external environmental samples, the influenza A virus positive rates in water, cage surface and fecal samples were 37.5% (6/16), 16.7% (5/30) and 18.8% (6/32), respectively. There were total 100 samples of serum whose A/Hubei/1/10 antigen inhibiting titers ≥ 40, accounting for 40.2%; while 36 samples of serum (14.5%) whose A/Anhui/1/05 antigen inhibiting titers ≥ 40 were found. The difference had statistical significance (χ(2) = 41.433, P < 0.05). Among the 249 serum samples collected from poultry-exposed population, 5 samples were H5N1 antibody positive against A/Hubei/1/10 antigen (inhibition titer ≥ 160), which came from 4 different live poultry markets, however, no positive serum sample against A/Anhui/1/05 antigen was found.

CONCLUSIONMultiple subtypes of avian influenza virus simultaneously prevailed in Wuhan urban poultry markets. Moreover, results from the distribution of avian influenza virus in external environment were consistent with the level of H5N1 antibody in poultry-exposed population.

Animals ; Antibodies, Viral ; blood ; Birds ; virology ; China ; Environment ; Humans ; Influenza A Virus, H5N1 Subtype ; immunology ; Occupational Exposure ; Poultry ; virology

The chitosan thermosensitive hydrogel is liquid at room temperature but gels rapidly when heated to body temperature. This hydrogel are wildly used for cell encapsulation, drug delivery or tissue-engineered scaffolds. The system can sustain the release of macromolecules over a period of several hours to a few days. However, with low-molecular-weight compounds, the release is generally completed within 24 h. To prepare a functional chitosan thermosensitive hydrogel for slow release both broad-spectrum antibiotic chlorhexidine and growth factor recombined human bone morphogenetic protein-2 (rhBMP-2), The beta-cyclodextrin was used to prepare an inclusion complex with chlorhexidine, and then the latter was incorporated into the chitosan thermosensitive hydrogel system. Simultaneously, rhBMP-2 was added into the hydrogel system. By HAAKE viscosity measuring instrument, we contrasted the viscoelastic properties of system with or without objective factors. And the in vitro release kinetics of chlorhexidine and rhBMP-2 was investigated by HPLC (high performance liquid chromatography) and ELISA (enzyme-linked immunosorbent assay) respectively. The results showed that the addition of chlorhexidine/beta-cyclodextrin inclusion complex to the thermosensitive solution did not change the gelling behavior of the thermosensitive system. Further, the in vitro release profiles demonstrated that the release rate of chlorhexidine and rhBMP-2 from hydrogel became slower, controlled delivery over at least 1 month. By first preparing chlorhexidine/beta-cyclodextrin inclusion complex, and then mixing the IC and rhBMP-2 into the chitosan thermosensitive hydrogel, a functional chitosan thermosensitive hydrogel system with ability of slow release both rhBMP-2 and chlorhexidine is successfully made.
Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins ; administration & dosage ; Chitosan ; chemistry ; Chlorhexidine ; administration & dosage ; Delayed-Action Preparations ; chemical synthesis ; Drug Carriers ; chemistry ; Drug Combinations ; Hydrogels ; chemistry ; Recombinant Proteins ; administration & dosage ; Temperature ; Transforming Growth Factor beta ; administration & dosage

This study was purposed to detect the expression of autophagy-related gene Beclin1 and microtubule-associated protein 1 light chain 3 (MAPLC3) in bone marrow mononuclear cells (BMMNC) isolated from acute leukemia (AL) patients, and to explore its significance. Transmission electron microscopy and RT-PCR were used to detect the autophagy activity and the expression level of Beclin1 and MAPLC3 mRNA in BMMNC isolated from 27 AL patients with de novo, refractory or relapse AL and completely remission and 31 normal persons respectively. The results showed that autophagy activity and expression levels of Beclin1 and MAPLC3 mRNA in BMMNC from de novo AL patients were 80%, 0.68 ± 0.18, 0.24 ± 0.06, respectively; those in BMMNC from refractory or relapse AL patients were 100%, 0.79 ± 0.09, 0.30 ± 0.07, respectively; those in BMMNC from CR patients were 40%, 0.52 ± 0.15, 0.16 ± 0.04, respectively, while those in BMMNC from normal persons were 20%, 0.57 ± 0.13, 0.16 ± 0.05, respectively. The autophagic activity and expression levels of Beclin1 and MAPLC3 mRNA in de novo and refractory or relapse AL patients were higher than those in normal persons, with statistical significance (p < 0.05), while the comparison between CR patients and normal control showed no statistical difference (p > 0.05). It is concluded that autophagy activity and Beclin1 and MAPLC3 mRNA expression level of in de novo and refractory or relapse patients are higher than those in normal control, and the up-regulation of autophagy activity and expression of Beclin1 and MAPLC3 mRNA in refractory or relapse patients is especially significant. This may be related to the genesis, development and drug resistance of AL.
Acute Disease ; Adolescent ; Adult ; Aged ; Apoptosis Regulatory Proteins ; metabolism ; Beclin-1 ; Bone Marrow Cells ; metabolism ; Child ; Female ; Humans ; Leukemia ; metabolism ; pathology ; Male ; Membrane Proteins ; metabolism ; Microtubule-Associated Proteins ; metabolism ; Middle Aged ; RNA, Messenger ; genetics ; Young Adult