1.Xanthone and Flavonoid Derivatives from the Leaves of Maclura tricuspidata with Antioxidant and Anti-tyrosinase Activity
Yang Hee JO ; Solip LEE ; Se Hwan RYU ; Sang Won YEON ; Ayman TURK ; Bang Yeon HWANG ; Mi Kyeong LEE
Natural Product Sciences 2021;27(4):234-239
Masclura tricuspidata, also called as Cudrania tricuspidata, is one of the most common Moraceae family plants in East Asia. Its trivial name follows mulberry due to the similar morphology. Investigation of the bioactive constituents of M. tricuspidata leaves yielded a new xanthone derivative along with twenty known compounds through various chromatographic techniques. A new compound was defined as mascluraxanthone (3), a prenylated xanthone glucoside on the basis of 1D and 2D NMR and MS data. Twenty known compounds were identified as four xanthone derivatives (1-2 and 4-5), two flavans (6-7), six flavanol derivatives (8-13), a flavonone (14) and seven flavonol derivatives (15-21). Among the isolated compounds, flavanol and flavonoid derivatives with 3′,4′-OH groups showed antioxidant and anti-tyrosinase activities. Conclusively, the leaves of M.tricuspidata are rich in aromatic compounds including xanthones and flavonoids. In addition, these constituents showed antioxidant and anti-tyrosinase potentials, which might be useful for oxidative stress related diseases.
2.Effect of Inducible Nitric Oxide Synthase Inhibitor on Apoptosis in Hypoxic-Ischemic Injury of the Neonatal Rat Brain: 1H Magnetic Resonance Spectroscopy Study.
Hyun Sook HWANG ; Ji Yeon BANG ; Pyung Hwan PARK
Korean Journal of Anesthesiology 2003;45(3):393-401
BACKGROUND: Inducible nitric oxide synthase (iNOS) is expressed during the late stage of ischemia and may play an important role in the delayed progression of ischemic brain injury. This study was conducted to investigate the effect of N-(3-[aminomethyl] benzyl] acetamidine (1,400 W), a selective inhibitor of iNOS on hypoxic ischemic injury in a neonatal rat model. METHODS: Seven-day old Sprague-Dawley rats were used. The right common carotid artery was ligated under halothane anesthesia. Three 3 hours after recovery, animals were exposed to 8% oxygen in 92% nitrogen. The treatment group (n = 14) received 7 intraperitoneal injection of 20 mg/kg of 1,400 W. The first dose was given 18 hours after the injury and interval between injections was 8 hours. The control group (n = 13) did not receive 1,400 W. The degree of ischemic damage and apoptosis were evaluated 3 days after injury by 1H magnetic resonance spectroscopy (MRS) and terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling (TUNEL) staining. The authors obtained Lipid/N-acetyl aspartate (Lip/NAA) and lipid/ creatine (Lip/Cr) ratios as apoptotic markers via 1H MRS, and counted the apoptotic cell number by TUNEL staining in the right hemisphere. Data were analyzed using the unpaired t-test. RESULTS: There were no significant differences in the Lip/NAA or Lip/Cr ratios of the 2 groups, and no significant differences were found in terms of the number of TUNEL positive cells between 2 groups, either. CONCLUSIONS: The iNOS inhibitor investigated did not show protective effects against delayed neuronal damage and apoptosis after the hypoxic-ischemic injury in newborn rats, at least during the 3 days following injury. Our results suggest that NO has different roles in cerebral hypoxia-ischemia according to the stage of neonatal cerebral development.
Anesthesia
;
Animals
;
Apoptosis*
;
Aspartic Acid
;
Brain Injuries
;
Brain*
;
Carotid Artery, Common
;
Cell Count
;
Creatine
;
Halothane
;
Humans
;
Hypoxia-Ischemia, Brain
;
In Situ Nick-End Labeling
;
Infant, Newborn
;
Injections, Intraperitoneal
;
Ischemia
;
Magnetic Resonance Spectroscopy*
;
Models, Animal
;
Neurons
;
Nitric Oxide Synthase Type II*
;
Nitrogen
;
Oxygen
;
Rats*
;
Rats, Sprague-Dawley
3.Chemical Constituents from Buddleja officinalis and Their Inhibitory Effects on Nitric Oxide Production.
Tae Wook PARK ; Chul LEE ; Jin Woo LEE ; Hari JANG ; Qinghao JIN ; Mi Kyeong LEE ; Bang Yeon HWANG
Natural Product Sciences 2016;22(2):129-133
Bioactivity-guided fractionation of a methanolic extract of Buddleja officinalis led to the isolation of two monoterpenes, crocusatin M (1), crocusatin C (2), a flavonoid, acacetin (3), three lignans, lariciresinol (4), pinoresinol (5), and syringaresinol (6), and two triterpenoidal saponins, mimengoside B (7) and songarosaponin A (8). The structures of isolates were identified based on 1D-, 2D-NMR, and MS data analysis. All isolates were tested for their inhibition on LPS-induced NO production in RAW 264.7 cells. As a result, mimengoside B (7) and songarosaponin A (8) showed a mild inhibitory activity of NO production.
Buddleja*
;
Lignans
;
Methanol
;
Monoterpenes
;
Nitric Oxide*
;
RAW 264.7 Cells
;
Saponins
;
Statistics as Topic
4.Ethanol extract of Angelica gigas inhibits croton oil-induced inflammation by suppressing the cyclooxygenase - prostaglandin pathway.
Sunhee SHIN ; Seong Soo JOO ; Dongsun PARK ; Jeong Hee JEON ; Tae Kyun KIM ; Jeong Seon KIM ; Sung Kyeong PARK ; Bang Yeon HWANG ; Yun Bae KIM
Journal of Veterinary Science 2010;11(1):43-50
The anti-inflammatory effects of an ethanol extract of Angelica gigas (EAG) were investigated in vitro and in vivo using croton oil-induced inflammation models. Croton oil (20 microgram/mL) up-regulated mRNA expression of cyclooxygenase (COX)-I and COX-II in the macrophage cell line, RAW 264.7, resulting in the release of high concentrations of prostaglandin E2 (PGE2). EAG (1~10 microgram/mL) markedly suppressed croton oil-induced COX-II mRNA expression and PGE2 production. Application of croton oil (5% in acetone) to mouse ears caused severe local erythema, edema and vascular leakage, which were significantly attenuated by oral pre-treatment with EAG (50~500 mg/kg). Croton oil dramatically increased blood levels of interleukin (IL)-6 and PGE2 without affecting tumor-necrosis factor (TNF)-alpha and nitric oxide (NO) levels. EAG pre-treatment remarkably lowered IL-6 and PGE2, but did not alter TNF-alpha or NO concentrations. These results indicate that EAG attenuates inflammatory responses in part by blocking the COX-PGE2 pathway. Therefore, EAG could be a promising candidate for the treatment of inflammatory diseases.
Angelica/*immunology
;
Animals
;
Cell Line
;
Cyclooxygenase 1/genetics/*immunology
;
Cyclooxygenase 2/genetics/*immunology
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Dinoprostone/genetics/immunology
;
Inflammation/drug therapy/enzymology/*immunology
;
Interleukin-6/blood
;
Macrophages
;
Male
;
Mice
;
Mice, Inbred ICR
;
Nitric Oxide/blood
;
Phytotherapy/*methods
;
Plant Extracts/*pharmacology/therapeutic use
;
Plant Roots/immunology
;
RNA, Messenger/chemistry/genetics
;
Reverse Transcriptase Polymerase Chain Reaction
;
Tumor Necrosis Factor-alpha/blood
5.A Systematic Review of Interventions with Siblings of Pediatric Cancer Patients.
Soo yeon HAN ; Ji Hye HWANG ; Cho hee KIM ; Hye young JANG ; Kyung Sook BANG
Child Health Nursing Research 2017;23(3):394-404
PURPOSE: The purpose of this study was to review interventions available to nurses caring for siblings of children with cancer. METHODS: Searches of CINAHL, MEDLINE, PubMed, EMBASE, and RISS identified ten intervention studies published from January, 2000 to November, 2016. RESULTS: Ten studies on interventions for siblings of pediatric cancer patients were identified as follows: 4 included camps, 4 included support groups, and 2 provided individual interventions. Theoretical frameworks were mainly cognitive behavioral theory and most studies were led by psychologists and multidisciplinary teams. The purpose of the interventions was primarily enhancing psychosocial adjustment. Siblings' fear of disease, self-esteem, and social support were improved significantly after the interventions. Findings were inconsistent with regard to depression, anxiety, behavioral problems, post-traumatic stress, health-related quality of life, and siblings' perceptions of the illness. CONCLUSION: Study findings showed the potential for enhancing emotional and behavioral outcomes in siblings of children with cancer. However, the number of studies was very small, and several methodological limitations were identified. In the future, more randomized controlled trials with larger samples are needed to extend the evidence base. Moreover, future research should identify sibling's characteristics and circumstances most likely to bring benefits to the siblings.
Anxiety
;
Child
;
Depression
;
Humans
;
Problem Behavior
;
Psychology
;
Quality of Life
;
Self-Help Groups
;
Siblings*
6.Falcarindiol from Angelica koreana Down-regulated IL-8 and Up-regulated IL-10 in Colon Epithelial Cells.
Sun Yup SHIM ; Seul gi LEE ; Mihye KIM ; Jin Woo LEE ; Bang Yeon HWANG ; Mina LEE
Natural Product Sciences 2017;23(2):103-107
Angelica koreana is an important medicinal plant for some locals in East Asia including Korea. A few reports have shown the efficacy of its phytochemical constituents. We have isolated and purified one compound falcarindiol (FAL) from the methanolic extract of A. koreana roots. At concentrations from to 1 µM to 25 µM, the FAL isolated from the roots of A. koreana exerted no significant cytotoxicity and down-regulated LPS-stimulated pro-inflammatory cytokine IL-8 in colon epithelial cells, while up-regulating anti-inflammatory cytokine IL-10. In addition, the FAL decreased the expression of LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 protein by Western blot analysis. Colon epithelial cells play pivotal roles in regulating the colon immune system and thus FAL is expected to be candidate agent as therapeutic potential for the treatment of inflammatory bowel disease (IBD) by modulating LPS-induced inflammation in colon epithelial cells.
Angelica*
;
Blotting, Western
;
Colon*
;
Epithelial Cells*
;
Far East
;
Immune System
;
Inflammation
;
Inflammatory Bowel Diseases
;
Interleukin-10*
;
Interleukin-8*
;
Korea
;
Methanol
;
Nitric Oxide Synthase Type II
;
Plants, Medicinal
;
Prostaglandin-Endoperoxide Synthases
7.Accuracy of Optic Disc Parameters Obtained by the Heidelberg Retina Tomograph in the Diagnosis of Glaucoma.
Jae Yeon JUN ; Jeong Hee HWANG ; Hyung Ju PARK ; Ki Bang UHM
Journal of the Korean Ophthalmological Society 2004;45(9):1503-1514
PURPOSE: To determine which optic disc topographic parameters, obtained by the Heidelberg Retina Tomograph (HRT), are most useful to distinguish normal eyes from those with early to moderate glaucomatous visual field defects. METHODS: From each of 92 normal subjects and 154 patients with primary open-angle glaucoma one randomly selected eye was studied. The Humphrey perimeter, program C 30-2 visual fields, and 13 HRT parameters (software 2.01, includes rim to disc area ratio) were utilized. Because some of the optic disc parameters depend on the optic disc area, these parameters were corrected for the effect of disc area. The total glaucoma group was divided into three subgroups according to the visual field defects: early (mean deviation [MD] >-6 dB, 65 eyes), moderate (MD -6 to -12 dB, 49 eyes), and severe (MD <-12 dB, 40 eyes) glaucoma groups. RESULTS: In eyes with early glaucoma (MD = -3.43 +/- 1.60 dB), the sensitivity, specificity, accuracy, and areas under the receiver operating characteristic curve were, respectively: rim area (69%, 71%, 70%, 0.802), rim to disc area ratio (69%, 72%, 71%, 0.799), cup area (72%, 70%, 71%, 0.789) and cup to disc area ratio (69%, 70%, 69%, 0.790). In eyes with moderate glaucoma (MD = -8.75 +/- 1.88 dB), they were, respectively: rim area (78%, 86%, 83%, 0.872), rim to disc area ratio (76%, 86%, 82%, 0.869), cup area (76%, 87%, 83%, 0.864) and cup to disc area ratio (78%, 84%, 82%, 0.862). There was considerable overlap in HRT parameters between the normal and early glaucoma groups. CONCLUSIONS: The diagnostic value of single optic disc parameter had its limitations in the diagnosis of early stage glaucoma.
Diagnosis*
;
Glaucoma*
;
Glaucoma, Open-Angle
;
Humans
;
Retina*
;
ROC Curve
;
Sensitivity and Specificity
;
Visual Fields
8.Antioxidant Activity and Phenolic Content of Different Parts of Lotus and Optimization of Extraction Condition using Response Surface Methodology
Jae Young JANG ; Jong Hoon AHN ; Yang Hee JO ; Bang Yeon HWANG ; Mi Kyeong LEE
Natural Product Sciences 2019;25(1):44-48
Nelumbo nucifera Gaertn. (Nymphaeaceae) is commonly called lotus and its leaves are widely been used as functional ingredients due to its antioxidant activity. For maximum efficacy, optimized extraction condition was established using response surface methodology. The high F-values, low p-values and insignificant p-value for lack-of-fit supported the fitness of the model and yielded the second-order polynomial regression for the antioxidant activity. The optimized extract was obtained by the extraction of 1 g of lotus leaves with 40 mL of 50% MeOH at 10.0℃, which exerted 70.1% antioxidant activity. Close correlation between phenolic content and antioxidant activity suggested phenolic compounds as active constituents of lotus leaves. In addition, comparison of different parts of lotus demonstrated the most potent antioxidant activity of flowers, followed by leaves and roots. Taken together, these results provide useful information about lotus leaves for the development as antioxidant ingredients. In addition, flowers and roots as well as leaves are suggested as good sources for antioxidant activity.
Flowers
;
Lotus
;
Nelumbo
;
Phenol
9.Anesthetic management of a patient with prune-belly syndrome : A case report.
Bo Young HWANG ; Yoo Kyung KIM ; Ji Hyun PARK ; Joon Young PARK ; Young Joo SEO ; Ji Yeon BANG ; Sung Moon JEONG ; Jai Hyun HWANG
Anesthesia and Pain Medicine 2009;4(1):50-54
Prune-belly syndrome is characterized by absent abdominal wall musculature with wrinkled overlying skin, urinary tract dilatation and cryptorchidism. Prune-belly syndrome is also associated with diseases of the respiratory, cardiovascular, skeletal, gastrointestinal and central nervous system. Because the congenital disease is uncommon, it is difficult to collect the information of anesthetic management of prune-belly syndrome. We report a case of 4 year-old-boy with prune-belly syndrome who underwent abdominoplasty and Mitrofanoff operation under general anesthesia.
Abdominal Wall
;
Abdominoplasty
;
Anesthesia, General
;
Central Nervous System
;
Cryptorchidism
;
Dilatation
;
Humans
;
Male
;
Prune Belly Syndrome
;
Skin
;
Urinary Tract
10.Optimization of Cultivation and Extraction Conditions of Pupae-Cordyceps for Cordycepin Production
Ayman TURK ; Beom Seok KIM ; Sung Min KO ; Sang Won YEON ; Se Hwan RYU ; Young-Guk KIM ; Bang Yeon HWANG ; Mi Kyeong LEE
Natural Product Sciences 2021;27(3):187-192
Cordycepin is a characteristic bioactive compound of Cordyceps militaris with various beneficial effects. Cordyceps grows on both grains and insects, and the content of cordycepin varies depending on the cultivation conditions. In this study, the effect of culture conditions on the cordycepin content was analyzed and the extraction conditions were optimized. Analysis of cordycepin content in Pupae-Cordyceps found that it was highly affected by temperature in culture conditions. In the case of mycelium, it grows well at 20 and 25 oC, but not at 30 oC. However, the content of cordycepin was highest at 30oC and less at 20 oC. The fruiting body also showed a similar tendency: growth was 20 oC > 25 oC > 30 oC, but the cordycepin content was 30 oC > 25 oC > 20 oC. The content of cordycepin decreased after the fruiting bodies were produced. Next, extraction conditions such as solvent and time were optimized for maximum cordycepin content using response surface methodology(RSM). There was a large difference in the content of cordycepin according to the content of ethanol and the extraction temperature. Through RSM, it was confirmed that the optimum condition for extraction of cordycepin was 48.9 oC using 49.0% ethanol, and 160.9 mg/g extract could be obtained under this condition. In conclusion, this study suggested the optimized conditions for the cultivation and extraction of Pupae-Cordyceps for maximizing the content of cordycepin, and this may be applied to the discovery of materials using cordycepin.