Objective To explore the clinical symptoms,signs,and analysis of misdiagnosis of blephaokera-toconjunctivitis(BKC).Methods The clinical data of 88 patients with BKC in our hospital from June 2014 to June 2016 were retrospectively analyzed.Based on the patients'symptoms,signs and BKC diagnostic criteria,the causes of misdiagnosis were analyzed.Results In 88 patients with BKC,59 cases were misdiagnosed,the misdiagnosis rate was 67.04%.The People's Hospital of Anshun misdiagnosed 10 cases(12.19%),among which resident misdiagnosed 6 cases(6.81%),attending physician misdiagnosed 2 cases (2.27%),deputy director and the above physician misdiagnosed 1 case (1.13%);outer court misdiagnosed 49 cases (55.68%),among which three level hospital misdiagnosed 8 cases(9.09%),II level hospital misdiagnosed 23 cases(26.13%),a level hospital and the following misdiagnosed 18 cases (20.45%);misdiagnosis disease including virus sex keratitis 14 cases(15.9%),chronic blepharitis in 13 cases(14.77%),the meibomian gland dysfunction(MGD)in 10 cases(11.36%),8 cases of dry eye(9.09%),6 cases of chronic conjunctivitis(6.81%),allergic conjunctivitis 5 cases (5.68%),marginal corneal ulcer in 3 cases(3.4%).Conclusion The clinical characteristics of BKC and viral keratitis,chronic blepharitis, MDGS and dry eye disease are similar and overlap,and clinicians are lack of knowledge and awareness of the disease, the clinical misdiagnosis and mistreatment is vulnerable,especially in primary care doctors.We should pay attention to the clinical patients'history,symptoms,slit lamp microscope examination of palpebral and conjunctival and corneal conditions,etc.thus to reduce misdiagnosis and avoid medical disputes.

Diabetic peripheral neuropathy (DPN) is the most common complication of diabetes mellitus in nervous system. However, the pathogenesis is still unkown. Therapeutic options are mainly pathogenetically oriented therapy and symptomatic therapy. With an increasing understanding of the pathogenesis of DPN, many new therapies have emerged. Comprehensive therapy targeting different pathogenesis will be frequently applied in the future. In this article, the common therapy and advance in treatment of DPN are reviewed.

Objective To study the effects of H_2S on the visceral pain induced by colorectal dis- tention (CRD) and the expression of substance P (SP)in rats.Methods Forty Wistar rats were randomly divided into control group,normal saline(i,p.) group,low( 15?mol/kg),moderate (45?mol/kg) and high(75?mol/kg) dose of NaSH(i.p.) groups.All rats.except control group,were subjected to CRD for 25 seconds and their behavioral responses to CRD were assessed by measuring the abdominal with drawal reflex(AWR).The expression of SP in the ileocecal junction,colon,spinal cord of thoracic waist (T6-L5) and brain was detected by immunohistochemistry.Results The visceral pain induced by CRD produced a capacity-dependent increase of AWR score in normal saline group,and a dose-dependent reduction of AWR score in three of NaSH groups.There was no significant differences of SP expression in the ileocecal junction,colon,spinal cord of thoracic waist and thalamencepbalon between the normal saline group and the NaSH low dose group.However,the decreased SP expression in the NaSH moder- ate and high dose groups were observed in comparison to those in the normal saline group(P

AIM:To be one of the primary cause injury to multiple sites of ocular of glaucoma which affects over 70 million people worldwide. We applied data mining techniques, linear and the matrix operations, efficiently calculated the network and estimated the possible function of the“node” genes of the retina and optic of glaucoma, in order to provide new thought and method on the pathogenesis of glaucoma.
METHODS: The data in this study is from Gene Expression Omnibus ( GEO ) which belong to Nation Center for Biotechnology Information ( NCBI) , the quality of the raw data CEL files was processed and analyzed by the Expression software which belong to Affymetrix Inc. , Santa Clare, CA, USA. Significant analysis method ( SAM) which base on the T test was used to identified the significant genes. Based on GRNInfer and Gvedit soft we set up gene networks of optic and retina of mice and further more enriched analysis which based on DAVID and MAS3. 0 online software were processed.
RESULTS:The analysis between the group of the optic nerve heads and retinas in different stage of glaucoma showed that the amount of significant different expressed genes in the optic never head group increased significantly comparing with the group of retina in the early stage of glaucoma, the analysis of the genes network construction show that:the node genes of optic nerve heads included Unc13c、Kif5a、TRPM1、PANX; and the node genes of retina include POU4F1, NEFL, BC03870, CALB2. Metabolic pathways enrichment analysis which based on MAS3. 0 online platform show that there was mainly the amyotrophic lateral sclerosis, tyrosine metabolism, melanogenesis, Nitrogen metabolism, Gap junction, Leukocyte transendothelial migration metabolism pathway enriched out in optic nerve head; and there was mainly amyotrophic lateral sclerosis, neurodegenerative disorders, prostate cancer, leukocyte transendothelial migration metabolism pathway enriched out in retina.
CONCLUSION:By understanding bioinformatics result, it seems optic were more sensitive than the retina to high intraocular pressure, and weather high expression of TYrp1 gene can be as a sensitive diagnostic item require more evidence back up. Functional enrich analysis of node gene showed that cytoskeleton reconstructed, molecular motor and nutrients transport function improve in optic; and in retina, the most prominent finding in retina was enrichment function modules were focus on regeneration, repairing and differentiation of cells, which remind that we should reinforce research on reparation of retina of primary glaucoma. Metabolic pathways enrichment analysis show that inflammatory response plays prominent place in optic and retina of primary glaucoma, because of the optic narrow and crowed anatomic shape, nutrient metabolism and substances transfer enrichment modules play an important role in optics of primary glaucoma.

Objective Studies have shown that low concentrations of nicotine can promote neovascularization and promote wound healing.This article aimed to investigate the influence of low concentration collagen membrane slow-release system on the hard palate trauma of rats.Methods Using poly(lactic acid-co-glycolic acid) (PLGA) copolymer as carrier materials, low concentration nicotine sustained-release particles were prepared by emulsion evaporation method (w/o/w), using collagen membrane as the brace and establish a low concentration collagen membrane system.48 Wistar rats were divided into experimental group and blank group, 3 mm diameter circular wound was made in the forepart palate.Low concentration of nicotine collagen membrane sustained-release particle system and blank collagen membrane (control) were sutured on the wound with 6-0 absorbable thread.Then, observed the wound healing of 0, 3, 7, 10 days and compared the healing differences between each groups.Results Under the electron microscope, the nicotine sustained-release particles were circular, similar size with rough surface, the average diameter were 3.0±0.2μm, the encapsulation efficiency and drug loading rate was 50.2% and 4.12% respectively.In vitro, nicotine sustained-release particles released much more nicotine on the first day, less on the second day, tends to stable and fluctuate within a certain range from the third day on, and declined sharply after about 10 days, nicotine concentration from 3rd to 10th day was fluctuate within 10-5-10-4mol/L.Postoperative wound healing, no significant difference in 3 days(P>0.05), after 7 days, the wound healing of experimental group significantly greater compared with the control (P=0.015).The wound was healed in 10 days after operative, there was no significant difference between two groups(P>0.05).The epithelial proliferation in the experimental group was significantly greater than that in the blank group, there were many fibroblasts, inflammatory cells and new capillaries, the epithelial process is short, the submucosa is loose, and a large number of collagen fibers are produced.The lamina propria is closely connected with the periosteum, and the wound is healed Conclusion Low concentration of nicotine sustained-release particles collagen membrane system may promote wound healing in the hard palate mucosa of rats.

Inoculant the examine-needed germ on a flat panel of synthetic medium, and placed paper slices, which had being saturated by different kinds of sugar, on the medium to carry out the experiment of microorganic auxanography. Its result was compared with that of the traditional method, the sugar-grain method. The comparison showed that the former was better than the latter for its more definite result and more convenient operation.

Objective To explore a good of treating posttraumatic deviated nose. Methods Clinical data of 136 patients with posttraumatic deviated nose were analyzed.Closed nasal bone replacement was employed in 34 patients with the disease history of 20-30 days,while open rhino- plasty approach was employed in 102 patients with the disease history over 6 months to correct their postt- raumatic deviated nose,and straightening the septum and ectomizing the inferior turbinate were done if necessary.Results The follow-up was over one year.In the 34 patients with the disease history of 20- 30 days,the outcome was excellent in 28 cases and good in 6.In the 102 patients with the disease history over 6 months,the outcome was excellent in 81 cases and good in 21.The deformity of nose was corrected satisfactorily.Normal nasal shape and good ventilation were obtained.Conclusion Posttraumatic devi- ated nose deformities are often caused by delayed and inaccurate treatment.Closed nasal bone replace- ment can be employed for the patients with trauma history less than one month,and open rhinoplasty ap- proach and straightening the septum and restoration of the nasal shape are employed for other patients.In this way good results can be obtained.

Fourty cases of postpartum heart failure occuring in Korean women in the Dept. of Int. Med., Pusan University Hospital are reported, with and analysis of clinical data including frequency, age, time of onset, clinical features, hemoglobin, liver function, electrocardiographic and chest X-ray findings. The following results were obtained. 1. The cases were mostly found in 20-29 years age group, 50% of the total cases. 2. Eighteen cases of fourty patients experienced the symptoms during the 2nd week after delivery and 12 cases complained the symptoms during 4th week. 3. Only six cases noted onset of heart disease following the 4th pregnancy, whereas sixteen patients experienced this phenomenon after the first gestation, eight patients after the 2nd gestation and ten patients after the 3rd pregnancy. 4. At the admission the patients were suffering from paroxysmal nocturnal dyspnea, orthopnea, exertional dyspnea and generalized edema. Physical findings revealed cardiomegaly, gallop rhythm, edema, pulmonary fine rales and systolic murmur in frequency. 5. Electrocardiograms were available at the time of presentation in thirty patients. Twenty four patients had left ventricular hypertrophy, 18 patients sinus tachycardia, 3 patients lower voltage. 6. The chest X-ray film showed the cardiomegaly in 15 cases, 83.3% of the obtained 18 cases, 12 cases pulmonary edema, 3 cases pericardial effusion. 7. The venous pressure was measured in 15 patients. In 5 cases the venous pressure was normal and in 10 increased above 150mmH2O. 8. With prompt treatment by rest, salt restriction, diuretics and digoxin the clinical symptoms improved in 31 cases but 9 patients died at the admission.
Busan ; Cardiomegaly ; Digoxin ; Diuretics ; Dyspnea ; Edema ; Electrocardiography ; Female ; Heart Diseases ; Heart Failure* ; Heart* ; Humans ; Hypertrophy, Left Ventricular ; Liver ; Pericardial Effusion ; Postpartum Period* ; Pregnancy ; Pulmonary Edema ; Respiratory Sounds ; Systolic Murmurs ; Tachycardia, Sinus ; Thorax ; Venous Pressure ; X-Ray Film

Objective To investigate the characteristics of craniocerebral injuries in the Beagle dogs caused by explosion shock wave and quasi-static pressure by simulating the condition of multiple cabins explosion in warship. Methods A total of 24 Beagle dogs were randomly divided into 2 groups, with 12 in each group, and the Beagle dogs of 2 groups were tested in the multiple cabins with the same size of the real warship. A 0. 65 kg TNT equivalent bare explosion was blasted in the cabins, which was defined as the target cabin (I group), and the neighboring one was defined as the adjacent cabin (II group). Parameters of shock waves were measured using pressure transducers. The survival status of dogs, basic vital signs, neurological function scores and the gross and morphological changes of the brains were observed after the explosion. Results There were two reflected shock waves after the explosion in the cabins, and the peak pressure of the shock wave in the adjacent cabin was about 0. 39 limes of that of the target cabin. Seven Beagle dogs died instantly after the explosion, and four died within 24 h. The mortality rale of the dogs was 45.83% (11/24). with that in the target cabin being 66. 67% (8/12) and that in the adjacent cabin being 25. 0% (3/12). The basic vital signs and neurological function of the survival dogs underwent greal changes immediately after the explosion, which basically recovered after 24 h. The brain issues of the dogs thai immediately died after explosion had obvious contusions, and cerebral hemorrhage and cerebral edema were found in the dogs died within 24 h after explosion. Abnormal morphological structures and neuronal structures were also found in the brain issues of survival Beagle dogs under light microscope, with some neurons showing nuclei condensation and deep staining, nuclear dissolution or disappearance of nucleus, and the cell borders being blurred. Conclusion In multiple cabin explosion, the peak pressure of the shock wave in the target cabin is significantly higher than that in the adjacent cabin. There are two reflected shock waves in the cabins, which increases the damage to the dogs. The mortality rate of the Beagle dogs in the target cabin is higher than that in the adjacent cabin; and the acute injuries of Beagle dogs immediately after the explosion are the most serious ones, with obvious pathological changes of the brain tissues.

【Objective】 To establish an enzyme-linked immunosorbent assay (ELISA) method for the determination of residual human coagulation factor Ⅺ in human prothrombin complex and validate the method. 【Methods】 Human factor Ⅺ was reacted with the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody was bound to the captured protein. Excess primary antibody was washed away and bound antibody was reacted with horseradish peroxidase conjugated streptavidin. TMB substrate was used for color development at 450 nm. The dilution reliability, accuracy, specificity, repeatability, intermediate precision, linearity, range and durability were verified. 【Results】 The verification results showed that the accuracy and specificity of this method met the experimental requirements, with an average recovery rate of 109.2% and RSD of 6.93%. The repeatability RSD was 6.78%, and the intermediate precision RSD was 6.75%, indicating good precision. The linear regression correlation coefficient of standard curve was 0.999 9, showing good accuracy and precision within the linear range. The durability was verified by the incubation time and the validity period of reagent kit opening. The results showed that the RSD of the incubation time change was 6.62%, indicating that the incubation time of this detection method was controlled between 28 to 32 minutes, and there was no significant impact on the results. The RSD of the detection results before and after the reagent kit was opened and stored under conditions for 7 days was 3.84%, indicating that the preservation of the reagent kit according to the conditions for 7 days after opening has no effect on the FⅪ detection results. Both indicated that the method had good durability. The dilution reliability results showed that there was a "hook" effect in the detection of FⅪ residue in human prothrombin complex, which could be solved by diluting 100 to 200 times. 【Conclusion】 This method can be used for the determination of FⅪ residues of human prothrombin complex in laboratory.