In an attempt to evaluate the expression of hepatitis B surface antigen(HBs Ag), transforming growth factor-ct(TGF-alpha), and proliferating cell nuclear antigen(PCNA) in hepatocellular carcinoma(HCC), an immunohistochemical investigation(ABC method) was performed using 31 surgically resected HCCS. The authors examined the expression rate and patterns, histopathologic correlation, and inter-relationships among these expressions. The results were summarized as follows. 1) Among 25 seropositive HCCS, 15 cases showed tissue expression of HBs Ag(60.0%), being expressed as a predominantly cytoplasmic pattern. Its expression rate in low grade HCC was significantly high(76.9%), in contrast to a low rate in high grade HCC(41.7%)(P<0.05). Adjacent nonenoplatic tissue showed a higher expression rate(82.6%). 2) TGF-alpha was expressed in 23 of 31 cases of HCC(74.3%). The intensity and extent of its expression did not correlate tyros with histopathologic features. Bile duct epithelium, juxtaposed and/or entrapped liver cells, and cirrhotic nodules were variably expressed, of which intense peripheral reaction within the nodules was frequently noted. 3) PCNA was expressed throughout the neoplastic tissue of HCC. Its index was significantly high(34.4 13.6), being compared to low index index(3.5 2. 1) in the nonneoplastic tissue(P<0.005). High grade tumors revealed a higher index than the low grade tumors(P<0.05). Conclusively, this data confirms that PCNA index offers useful information about cell proliferation associated with histologic degrees of malignancy of HCC, albeit TGF-alpha is also involved in cellular proliferation of both liver cell and bile duct epithelium. Changes in incidence and cellular localization of HBs Ag expression between the neoplastic and nonneoplastic tissues suggest that an integrated viral genome could be functionally altered during hepatocarcinogenesis. A significant inter-relationship among these expressions was not observed.
Incidence ; Carcinoma, Hepatocellular

Cellular altered foci (CAF), hyperplastic nodules (HN) including regenerating and adenomatous nodules, and hepatocellular carcinomas (HCC) were induced in Sprague-Dawley rat liver by prolonged administration of N-diethylnitrosamine (DEN, 200 ppm). Immunohistochemical expression of connexin 32 (Cx 32), cyclin-dependent kinase 4 (CDK 4), and proliferating cell nuclear antigen (PCNA) was assessed for the evaluation of preneoplastic potential of CAF. Regardless the duration of DEN administration, basophilic cell foci were the most frequently observed lesion in both CAF and cellular expanding hyperplastic nodules. Eosinophilic cell foci, however, were concomitantly increased with adenomatous nodules in later experimental groups. Cx 32 showed perimembranous spot-like expression. Its number was 7.25 2.10 per cell in normal hepatocytes. CAF and adenomatous nodules showed markedly decreased Cx 32 spots. Moreover, its reduction was more prominent in HCC. PCNA-labelled hepatocytes were scattered in the most CAF, showing no significant difference between each CAF. PCNA labelling index (LI) in adenomatous nodule and HCC was markedly increased. CDK 4 was localized in the cytoplasm and/or nucleus of hepatocytes. Eosinophilic cell foci revealed more nuclear expression of CDK 4 than other types of CAF, of which expression incidence was comparable to that of adenomatous nodule. Nuclear CDK 4 expression in HN and HCC was increased, although significant difference between regenerating nodule and adenomatous nodule was not seen. In conclusion, the incidence of CDK 4 was concomitantly increased with PCNA LI, however, reciprocally decreased with Cx 32 in accordance with the advance of DEN-induced HCC in rat. Phenotypically altered foci manifested as CAF are early valuable preneoplastic marker lesion for evaluation. In addition, basophilic cell foci can be considered a discernible marker of cellular expansion within nodules. However, eosinophilic cell foci might be an indeterminate marker for the advance of DEN-induced HCC in rat.
Animals ; Basophils ; Carcinoma, Hepatocellular ; Cyclin-Dependent Kinase 4 ; Cytoplasm ; Eosinophils ; Hepatocytes ; Incidence ; Liver* ; Proliferating Cell Nuclear Antigen* ; Rats* ; Rats, Sprague-Dawley

Perivascular epithelioid cell tumor (PEComa) is a mesenchymal tumor consisting of distinctive perivascular epithelioid cells, and is commonly detected in the uterus. The liver is an uncommon site for primary PEComa. In this study, we report a case of primary hepatic PEComa in a 36-year-old woman. Upon gross examination, the tumor was a well-defined, brownish solid mass, measuring 6.5x5.2x4.5 cm. Microscopically, the tumor consisted largely of epithelioid cells and some spindle cells with a clear to eosinophilic cytoplasm and a rich network of delicate capillaries in the stroma. With the exception of their relatively large size and microscopically sinusoidal infiltrative growth pattern, all other histopathologic features of the tumor were consistent with their being benign. The tumor cells were positive for human melanoma black-45 and smooth muscle actin, and negative for cytokeratin-cocktail and c-kit.
Actins ; Adult ; Capillaries ; Cytoplasm ; Eosinophils ; Epithelioid Cells ; Female ; Humans ; Liver ; Melanoma ; Muscle, Smooth ; Perivascular Epithelioid Cell Neoplasms ; Uterus

Perivascular epithelioid cell tumor (PEComa) is a mesenchymal tumor consisting of distinctive perivascular epithelioid cells, and is commonly detected in the uterus. The liver is an uncommon site for primary PEComa. In this study, we report a case of primary hepatic PEComa in a 36-year-old woman. Upon gross examination, the tumor was a well-defined, brownish solid mass, measuring 6.5x5.2x4.5 cm. Microscopically, the tumor consisted largely of epithelioid cells and some spindle cells with a clear to eosinophilic cytoplasm and a rich network of delicate capillaries in the stroma. With the exception of their relatively large size and microscopically sinusoidal infiltrative growth pattern, all other histopathologic features of the tumor were consistent with their being benign. The tumor cells were positive for human melanoma black-45 and smooth muscle actin, and negative for cytokeratin-cocktail and c-kit.
Actins ; Adult ; Capillaries ; Cytoplasm ; Eosinophils ; Epithelioid Cells ; Female ; Humans ; Liver ; Melanoma ; Muscle, Smooth ; Perivascular Epithelioid Cell Neoplasms ; Uterus

Primary carcinoid tumor of the kidney is extremely rare ; only 29 previous reports have been described in the world literature, 3 cases of which were known to arise in horseshoe kidneys. Because of the scarcity of primary renal carcinoid tumor, its clinicopathologic features and prognosis are not well characterized, and its histogenesis is unknown. We present a case of primary carcinoid tumor arising in the horseshoe kidney of a 43-year-old man. The lesion is morphologically identical to those of gastrointestinal or respiratory tracts. In addition, this tumor contains clusters of intestinal epithelium with goblet cells and mature bony trabeculae. The cytoplasm of the tumor cells are immunoreactive for keratin, NSE, and synaptophysin, but are negative with Grimelius and Fontana-Masson stains. Membrane-bound cytoplasmic neurosecretory granules are observed by electron microscopic examination. This is the first case of primary renal carcinoid tumor arising in a horseshoe kidney documented in Korea. We describe the gross features, light and electron microscopic, and immunohistochemical findings of renal carcinoid and review the literature, with speculations on the possible histogenesis of this unusual neoplasm.

Intercellular adhesion molecule I(ICAM-1; CD 54), a 90 kD glycoprotein, counter-receptor for lymphocyte function-associated antigen-I(LFA-1) on T-cells, is critically important to a wide variety of adhesion-dependent leukocyte functions, including antigen presentation and target cell lysis. Induction of ICAM-1 on the keratinocytes(KCs) is an important regulator in initiation, maintenance, and resolution of cutaneous inflammation, which is modulated with cytokines produced by activated T-lymphocytes. This study was designed to further our understanding on modulation effects of ultraviolet B(UVB), gamma interferon(IFN-;v), and retinoic acid(all trans) upon expression of ICAM-1 on cultured human KCs, with emphasis on their correlation. Cell surface expression of ICAM-1 in cultured human KCs was analyzed with the use of indirect immunofluorescence and fluorescence activating cell sorting(FACS) by flow cytometry. The results of this study were as follows: 1) Expression of ICAM-1 was significantly induced with IFN-,-(20 U/ml)(p<0.005). 2) UVB irradiation of 30mJ/cm2 significantly suppressed ICAM-1 expression of KCs 24 hours after irradiation(p<0.05). However, at 72 hours after irradiation, ICAM-1 expression of KCs was considerably increased in comparison to that of initial phase (24 hours after irradiation). 3) High concentrations(10(-5)M) of retinoic acid reduced UVB-induced expression of ICAM-1 in late phase(72 hours after irradiation), although retinoic acid showed induction effect of ICAM- I expression of KCs. In summary, these results indicate that ICAM- I may contribute to the biphasic effect of UVB on delayed hypersensitivity in vivo. Also, retinoic acid, a vitamin A derivative, may have a cutaneous photoprotective effect through a regulation of UVB-induced ICAM-1 expression on the KCs.
Humans

Tinea faeiei is an unusual fungal infection which often is not recognized by clinieians. Because of light sensitivity, the frequent absence of typical clinical manifestation, and secondary changes due to steroid treatment, this condition is frequently misdiagnosed with other dermatoses. We described two patients who showed atypical facial lesions which were histologically diagnosed as tinea faciei. The case I was a 54 year old male who had multiple discoid plaques and nodules on the face. The other case was a 83 year old female who showed scaly plaques, nodules, pustules, and photosensitivity on the face. Histologic exarnination revealed fungus spores and mycelia within the inflamed hair follicles.
Aged, 80 and over ; Female ; Fungi ; Hair Follicle ; Humans ; Male ; Middle Aged ; Photophobia ; Skin Diseases ; Spores ; Tinea*

Clear Cell Sarcoma of the Kidney(CCSK) is a rare malignant childhood tumor which is distinguished from Wilms tumor by its pathologic features, clinical presentation and frequent occurrence of metastasis to bone. We report a case of CCSK from a 2 year-old girl in the right kidney, followed by metastasis to thoracic vertebrae and left temporal lobe. Histogenesis of this tumor is controversial, although some studies suggest primitive mesenchymal origin. This case was studied with the aids of immunohistochemistry and electron microscopy in an effort to verify the histogenesis of the tumor. Vimentin was reactive in tumor cell, but cytokeratin, GFAP, S-100 protein and desmin were not stained, which confirmed the previous reports by others. Ultrastructural observation of the tumor cells showed neither features of epithelial cell nor differentiated mesenchymal cells.
Child ; Male ; Female ; Humans ; Neoplasm Metastasis

The inflammatory pseudotumor of the lung is a non-neoplastic pulmonary mass lesion, composed of a variey of inflammatory cells including plasma cells, histiocytes(often xanthomatous), mast cells, lymphocytes, and spindle shaped mesenchymal cells. Although the pathogenetic etiology of this lesion is not estabilished, it has been claimed that it is associated with local inflammatory reaction. From 1984 to 1986, we experienced three cases of pathologically confirmed inflammatory pseudotumor of the lung. All three cases revealed similar gross and microscopic features, with only minor differences in components on microscopic level. All specimens were products of lobectomy. They showed a relatively well defined, yellowish white and solid mass, measuring about 5.0 cm in diameter. Histologically, the lesions, which were well demarcated from the uninvolved region, were characterized by dense infiltration of plasma cells and xanthomatous histiocytes admixed with lymphocytes, spindle shaped mesenchymal cells and polymorphonuclear leukocytes. At periphery, bronchi and bronchioles were entrapped by these cells. In focal areas, spindle shaped mesenchymal cells were aggregated, resulting in formation of thick bundles in which plasma cells were infiltrated. In case 1 and 2, myxoid change of stromal tissue was noted. Also noted were foci of osteoid metaplasia of the stromal layer in case 1. The authors report three cases of inflammatory pseudotumor of the lung, with comparative observation of each case, associated with literature review, with emphasis on the pathogenesis of this rather infrequent lesion. And some reviews were made on differential diagnosis between inflammatory pseudotumor and non-neoplastic or neoplastic lung mass including so called sclerosing hemangioma.
Diagnosis, Differential

The inflammatory pseudotumor of the lung is a non-neoplastic pulmonary mass lesion, composed of a variey of inflammatory cells including plasma cells, histiocytes(often xanthomatous), mast cells, lymphocytes, and spindle shaped mesenchymal cells. Although the pathogenetic etiology of this lesion is not estabilished, it has been claimed that it is associated with local inflammatory reaction. From 1984 to 1986, we experienced three cases of pathologically confirmed inflammatory pseudotumor of the lung. All three cases revealed similar gross and microscopic features, with only minor differences in components on microscopic level. All specimens were products of lobectomy. They showed a relatively well defined, yellowish white and solid mass, measuring about 5.0 cm in diameter. Histologically, the lesions, which were well demarcated from the uninvolved region, were characterized by dense infiltration of plasma cells and xanthomatous histiocytes admixed with lymphocytes, spindle shaped mesenchymal cells and polymorphonuclear leukocytes. At periphery, bronchi and bronchioles were entrapped by these cells. In focal areas, spindle shaped mesenchymal cells were aggregated, resulting in formation of thick bundles in which plasma cells were infiltrated. In case 1 and 2, myxoid change of stromal tissue was noted. Also noted were foci of osteoid metaplasia of the stromal layer in case 1. The authors report three cases of inflammatory pseudotumor of the lung, with comparative observation of each case, associated with literature review, with emphasis on the pathogenesis of this rather infrequent lesion. And some reviews were made on differential diagnosis between inflammatory pseudotumor and non-neoplastic or neoplastic lung mass including so called sclerosing hemangioma.
Diagnosis, Differential