1.Isolation and molecular characterization of Brucella melitensis from seropositive goats in Peninsula Malaysia
Bamaiyi, P.H. ; Hassan, L. ; Khairani-Bejo, S. ; Zainal Abidin, M. ; Ramlan, M. ; Krishnan, N. ; Adzhar, A. ; Abdullah, N. ; Hamidah, N.H.M. ; Norsuhanna, M.M. ; Hashim, S.N.
Tropical Biomedicine 2012;29(4):513-518
A study was carried out to isolate Brucella melitensis using established
bacteriological and PCR techniques in Brucella seropositive goats in farms in Selangor, Negeri
Sembilan, Melaka and Pulau Pinang. Brucella melitensis was isolated from 7 of 134 reactors
with the highest isolation from the vaginal swabs (57.14%) followed by the spleen (28.57%),
uterine fluid (14.29%). No Brucella was isolated from the lymph nodes. PCR confirmed all the
seven isolates as B. melitensis and isolates were phylogenetically related to other isolates
from India, Iran, and Israel but most closely related to isolates from Singapore.
Result Analysis
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